The present study aims to make an evaluation of some secondary metabolites and determination of the antioxidant potential of <i>P. aquilinum</i> plant extracts obtained by means of a simple and rapid TLC m...The present study aims to make an evaluation of some secondary metabolites and determination of the antioxidant potential of <i>P. aquilinum</i> plant extracts obtained by means of a simple and rapid TLC method. The latter revealed the presence of terpenes, sterols, steroids, flavonoids, polyphenols, saponins, sugars and amino acids. The evaluation of the potential antioxidant was assessed on phenolic and flavonoid compounds. These compounds’ dosages revealed different levels, but the highest antioxidant activity was found in the hydro-ethanol extract followed by the aqueous extract. Among the two families of evaluated antioxidants, phenolic compounds were found to be higher in the hydro-ethanolic extract (75.18 mgEAG/gM<sub>S</sub>), followed by the aqueous extract (66.78 mgEAG/gM<sub>S</sub>) and lower in the ethanolic extract (12.39 mgEAG/gM<sub>S</sub>). Whereas flavonoids, less significantly elevated, showed values of 2.58 mgECa/gM<sub>S</sub> for the hydro-ethanolic extract, 2.24 mgECa/gM<sub>S</sub> for the aqueous extract and 1.58 mgECa/gM<sub>S</sub> for the ethanolic extract. However, the antiradical activity was also evaluated. Contrary to the antioxidant activity, the most important antiradical activity was observed on the hydroethanolic extract with a rate of 3.61 mg/mL, then a weak activity on the aqueous and ethanolic extracts respectively 6.18 mg/mL and 15.81 mg/mL, then less important on the aqueous and hydro-ethanolic extracts respectively at levels of 6.18 mg/mL and 3.61 mg/mL.展开更多
[Objectives]To explore the antidepressant-like effects of PAFL and its potential mechanisms.[Methods]This study used PAFL in mice under various conditions and analyzed the results.The antidepressant-like effects of fl...[Objectives]To explore the antidepressant-like effects of PAFL and its potential mechanisms.[Methods]This study used PAFL in mice under various conditions and analyzed the results.The antidepressant-like effects of flavonoids from Pteridium aquilinum(PAFL)and its possible action mechanism were discussed.[Results]PAFL(5 mg/kg,i.p.)exhibited markedly antidepressant-like activities,which could be reversed by treatment with p-chlorophenylalanine(an inhibitor of 5-HT synthesis),haloperidol(a non-selective D 2 receptor antagonist)and N-methyl-D-aspartic acid(an agonist at the glutamate site).Meanwhile,PAFL also effectively increased the hippocampus 5-HT,DA,and Glu levels of mice exposed to TST and FST.The LPS-induced antidepressant behavioral experiment showed that PAFL(1 and 5 mg/kg,i.p.)and fluoxetine(20 mg/kg,i.p.)effectively reduced the immobility time,and increase activity time in OFT.PAFL pretreatment significantly reduced IL-6 and TNF-αserum levels and improve the changes of hippocampal SOD and MDA oxidative stress indicators.Furthermore,PAFL preconditioning could up-regulate BDNF and TrκB contents in hippocampus and down-regulate IκB-αand NF-κB phosphorylation.[Conclusions]The antidepressant-like effects of PAFL might be mediated by the 5-HT,DAergic and Gluergic systems,and the mechanisms of anti-depressant effects of PAFL might be via the alterations of animal behaviors,hippocampus inflammation and neurotrophy,which might be attributed by the BDNF/TrκB/NF-κB signaling pathway.展开更多
[Objectives]To explore the protective effects of flavonoids from Pteridium aquilinum(PAFL)on carbon tetracholoride(CCl_(4))-induced acute liver injury in mice and its potential mechanism.[Methods]All mice were randoml...[Objectives]To explore the protective effects of flavonoids from Pteridium aquilinum(PAFL)on carbon tetracholoride(CCl_(4))-induced acute liver injury in mice and its potential mechanism.[Methods]All mice were randomly divided into four groups(n=10 in each),normal group,CCl_(4)group,CCl_(4)+PAFL groups[treated with PAFL(50 or 200 mg/kg)].Animal treatment was continued for 7 consecutive days.The blood was collected after injection of CCl_(4)for 24 h,and the liver tissue was removed from the mice and stored at-80℃.[Results]The PAFL(50 and 200 mg/kg)significantly inhibited the increase of aspartate aminotransferase(AST)and alanine aminotransferase(ALT)levels in serum caused by CCl_(4)treatment.PAFL administration not only increased the activity of antioxidant enzymes superoxide dismutase(SOD),Glutathione(GSH)and catalase(CAT)in mice,but also reduced the level of malondialdehyde(MDA).Meanwhile,PAFL administration decreased the expression of nuclear factor-kappa B(NF-κB)and Cyclooxygenase-2(COX-2)proteins and inhibited the release of pro-inflammatory factors tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β)and interleukin 6(IL-6).In addition,PAFL(200 mg/kg)treatment down-regulated extracellular regulated protein kinases(ERK)and c-Jun N-terminal kinase(JNK)protein levels in liver tissue.[Conclusions]These findings clearly indicate that the protective effects of PAFL on CCl_(4)-induced acute liver injury is related to its antioxidant and anti-inflammatory activity,which may be mediated by NF-κB and MAPKs signaling pathways.展开更多
本研究使用Illumina Hi Seq平台通过转录组测序对蕨菜可食用期的茎(NJ)和蜷缩叶(JY)以及不可食用期的老茎(LJ)和老叶(LY)样本进行序列分析,获得了26.56 Gb数据,76427个unigene,序列平均长度为1013 bp,其中42177(55.19%)个unigene在Nr、N...本研究使用Illumina Hi Seq平台通过转录组测序对蕨菜可食用期的茎(NJ)和蜷缩叶(JY)以及不可食用期的老茎(LJ)和老叶(LY)样本进行序列分析,获得了26.56 Gb数据,76427个unigene,序列平均长度为1013 bp,其中42177(55.19%)个unigene在Nr、Nt、Swiss-Prot、KEGG、KOG、InterPro和GO数据库获得注释信息。重点分析了蕨菜中与萜类生物合成相关途径unigene133个,主要参与萜类骨架生物合成、单萜化合物生物合成、倍半萜化合物生物合成、二萜和四萜化合物生物合成。其中,NJ与JY比对,差异显著表达基因47个,NJ与LJ比对,差异显著表达基因35个,NJ与LY比对,差异显著表达基因43个。这些基因为进一步深入挖掘蕨菜功能基因、研究萜类化合物等天然活性成分和天然毒素等生物合成与代谢调控分子机制提供依据。展开更多
采用树脂及正反相硅胶色谱柱从蕨菜 (Pteridiumaquilinumvar .latiusculum)中分离纯化出 9个化合物 ,通过波谱分析将它们分别鉴定为胡萝卜苷、山奈酚 3 O (6″ O 反 对羟基苯丙烯酰基 ) β D 葡萄糖苷、腺嘌呤核苷、蕨素 3 O β D ...采用树脂及正反相硅胶色谱柱从蕨菜 (Pteridiumaquilinumvar .latiusculum)中分离纯化出 9个化合物 ,通过波谱分析将它们分别鉴定为胡萝卜苷、山奈酚 3 O (6″ O 反 对羟基苯丙烯酰基 ) β D 葡萄糖苷、腺嘌呤核苷、蕨素 3 O β D 葡萄糖苷、牛膝甾酮、芦丁、蕨素A、坡那甾酮A和槲皮素。其中腺嘌呤核苷和牛膝甾酮为首次从该种植物中分离得到。展开更多
考察干燥因素对蕨菜真空干燥生产率及其品质的影响,用二次正交旋转组合设计的试验方法,探讨干燥温度、真空度、物料层厚度对蕨菜营养成分维生素C含量的影响规律。利用单、双因素分析法分析各因素与试验指标的关系,确定各因素在二次非线...考察干燥因素对蕨菜真空干燥生产率及其品质的影响,用二次正交旋转组合设计的试验方法,探讨干燥温度、真空度、物料层厚度对蕨菜营养成分维生素C含量的影响规律。利用单、双因素分析法分析各因素与试验指标的关系,确定各因素在二次非线形模型中的主次顺序。试验结果分析表明,真空度对蕨菜制品的维生素C含量影响最大,干燥温度次之,物料层厚度的影响最小。通过优化计算,确定了温度在58.7℃、真空度为0.072 M Pa、物料厚度为12 mm时,蕨菜的维生素C含量高。展开更多
文摘The present study aims to make an evaluation of some secondary metabolites and determination of the antioxidant potential of <i>P. aquilinum</i> plant extracts obtained by means of a simple and rapid TLC method. The latter revealed the presence of terpenes, sterols, steroids, flavonoids, polyphenols, saponins, sugars and amino acids. The evaluation of the potential antioxidant was assessed on phenolic and flavonoid compounds. These compounds’ dosages revealed different levels, but the highest antioxidant activity was found in the hydro-ethanol extract followed by the aqueous extract. Among the two families of evaluated antioxidants, phenolic compounds were found to be higher in the hydro-ethanolic extract (75.18 mgEAG/gM<sub>S</sub>), followed by the aqueous extract (66.78 mgEAG/gM<sub>S</sub>) and lower in the ethanolic extract (12.39 mgEAG/gM<sub>S</sub>). Whereas flavonoids, less significantly elevated, showed values of 2.58 mgECa/gM<sub>S</sub> for the hydro-ethanolic extract, 2.24 mgECa/gM<sub>S</sub> for the aqueous extract and 1.58 mgECa/gM<sub>S</sub> for the ethanolic extract. However, the antiradical activity was also evaluated. Contrary to the antioxidant activity, the most important antiradical activity was observed on the hydroethanolic extract with a rate of 3.61 mg/mL, then a weak activity on the aqueous and ethanolic extracts respectively 6.18 mg/mL and 15.81 mg/mL, then less important on the aqueous and hydro-ethanolic extracts respectively at levels of 6.18 mg/mL and 3.61 mg/mL.
基金Supported by the Innovation Project of Jilin Academy of Agricultural Sciences(cxgc2017zy011)Major Project of Jilin Province Science and Technology Department(20170204046ny).
文摘[Objectives]To explore the antidepressant-like effects of PAFL and its potential mechanisms.[Methods]This study used PAFL in mice under various conditions and analyzed the results.The antidepressant-like effects of flavonoids from Pteridium aquilinum(PAFL)and its possible action mechanism were discussed.[Results]PAFL(5 mg/kg,i.p.)exhibited markedly antidepressant-like activities,which could be reversed by treatment with p-chlorophenylalanine(an inhibitor of 5-HT synthesis),haloperidol(a non-selective D 2 receptor antagonist)and N-methyl-D-aspartic acid(an agonist at the glutamate site).Meanwhile,PAFL also effectively increased the hippocampus 5-HT,DA,and Glu levels of mice exposed to TST and FST.The LPS-induced antidepressant behavioral experiment showed that PAFL(1 and 5 mg/kg,i.p.)and fluoxetine(20 mg/kg,i.p.)effectively reduced the immobility time,and increase activity time in OFT.PAFL pretreatment significantly reduced IL-6 and TNF-αserum levels and improve the changes of hippocampal SOD and MDA oxidative stress indicators.Furthermore,PAFL preconditioning could up-regulate BDNF and TrκB contents in hippocampus and down-regulate IκB-αand NF-κB phosphorylation.[Conclusions]The antidepressant-like effects of PAFL might be mediated by the 5-HT,DAergic and Gluergic systems,and the mechanisms of anti-depressant effects of PAFL might be via the alterations of animal behaviors,hippocampus inflammation and neurotrophy,which might be attributed by the BDNF/TrκB/NF-κB signaling pathway.
基金the Innovation Project of Jilin Academy of Agricultural Sciences(CXGC2017ZY011)Major Project of Jilin Provincial Department of Science and Technology(20170204046NY)。
文摘[Objectives]To explore the protective effects of flavonoids from Pteridium aquilinum(PAFL)on carbon tetracholoride(CCl_(4))-induced acute liver injury in mice and its potential mechanism.[Methods]All mice were randomly divided into four groups(n=10 in each),normal group,CCl_(4)group,CCl_(4)+PAFL groups[treated with PAFL(50 or 200 mg/kg)].Animal treatment was continued for 7 consecutive days.The blood was collected after injection of CCl_(4)for 24 h,and the liver tissue was removed from the mice and stored at-80℃.[Results]The PAFL(50 and 200 mg/kg)significantly inhibited the increase of aspartate aminotransferase(AST)and alanine aminotransferase(ALT)levels in serum caused by CCl_(4)treatment.PAFL administration not only increased the activity of antioxidant enzymes superoxide dismutase(SOD),Glutathione(GSH)and catalase(CAT)in mice,but also reduced the level of malondialdehyde(MDA).Meanwhile,PAFL administration decreased the expression of nuclear factor-kappa B(NF-κB)and Cyclooxygenase-2(COX-2)proteins and inhibited the release of pro-inflammatory factors tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β)and interleukin 6(IL-6).In addition,PAFL(200 mg/kg)treatment down-regulated extracellular regulated protein kinases(ERK)and c-Jun N-terminal kinase(JNK)protein levels in liver tissue.[Conclusions]These findings clearly indicate that the protective effects of PAFL on CCl_(4)-induced acute liver injury is related to its antioxidant and anti-inflammatory activity,which may be mediated by NF-κB and MAPKs signaling pathways.
文摘本研究使用Illumina Hi Seq平台通过转录组测序对蕨菜可食用期的茎(NJ)和蜷缩叶(JY)以及不可食用期的老茎(LJ)和老叶(LY)样本进行序列分析,获得了26.56 Gb数据,76427个unigene,序列平均长度为1013 bp,其中42177(55.19%)个unigene在Nr、Nt、Swiss-Prot、KEGG、KOG、InterPro和GO数据库获得注释信息。重点分析了蕨菜中与萜类生物合成相关途径unigene133个,主要参与萜类骨架生物合成、单萜化合物生物合成、倍半萜化合物生物合成、二萜和四萜化合物生物合成。其中,NJ与JY比对,差异显著表达基因47个,NJ与LJ比对,差异显著表达基因35个,NJ与LY比对,差异显著表达基因43个。这些基因为进一步深入挖掘蕨菜功能基因、研究萜类化合物等天然活性成分和天然毒素等生物合成与代谢调控分子机制提供依据。
文摘采用树脂及正反相硅胶色谱柱从蕨菜 (Pteridiumaquilinumvar .latiusculum)中分离纯化出 9个化合物 ,通过波谱分析将它们分别鉴定为胡萝卜苷、山奈酚 3 O (6″ O 反 对羟基苯丙烯酰基 ) β D 葡萄糖苷、腺嘌呤核苷、蕨素 3 O β D 葡萄糖苷、牛膝甾酮、芦丁、蕨素A、坡那甾酮A和槲皮素。其中腺嘌呤核苷和牛膝甾酮为首次从该种植物中分离得到。
文摘考察干燥因素对蕨菜真空干燥生产率及其品质的影响,用二次正交旋转组合设计的试验方法,探讨干燥温度、真空度、物料层厚度对蕨菜营养成分维生素C含量的影响规律。利用单、双因素分析法分析各因素与试验指标的关系,确定各因素在二次非线形模型中的主次顺序。试验结果分析表明,真空度对蕨菜制品的维生素C含量影响最大,干燥温度次之,物料层厚度的影响最小。通过优化计算,确定了温度在58.7℃、真空度为0.072 M Pa、物料厚度为12 mm时,蕨菜的维生素C含量高。
