A new hemiterpenoid, （＋）-（2R,3S）-2-chloro-3-hydroxy-3-methyl-T-buty-rolactone was isolatexi from the leaves of Prinsepia utilis Royle. Its structure was clucidatexi by spectroscopic methods and X-ray crystallogra...A new hemiterpenoid, （＋）-（2R,3S）-2-chloro-3-hydroxy-3-methyl-T-buty-rolactone was isolatexi from the leaves of Prinsepia utilis Royle. Its structure was clucidatexi by spectroscopic methods and X-ray crystallographic analysis.展开更多
The purpose of this study is to isolate the active fraction from the crude extract of Prinsepia utilis Royle and to determine its antibacterial activity against the standard and drug-resistant bacteria strains in vitr...The purpose of this study is to isolate the active fraction from the crude extract of Prinsepia utilis Royle and to determine its antibacterial activity against the standard and drug-resistant bacteria strains in vitro. The Prinsepia utilis Royle was extracted in three portions by using petroleum ether, ethanol and water by the continuation recirculate extraction. The antibacterial activity of the extracts were evaluated by the determination of Minimum Inhibitory Concentration （MIC） and Minimum Bactericidal Concentration （MBC） values. The results showed that the ethanol extract and aqueous extract of petroleum ether showed the weakest inhibition. MIC values of the aqueous Prinsepia utilis Royle were found that they had significant effects against bacteria, while ethanol extracts against three standard bacteria （S. aureus ATCC25923, E. coli 44102, Salmonella 50041） were 25, 25, 50 mg·mL^-1 and 50, 100, 100 mg·mL^-1, MIC values of three drug-resistant bacteria （S. aureus GL17, E. coli EYAC08-56, Salmonella STQD2G.） were 50, 50, 50 mg·mL^-1 and 100, 100, 200 mg·mL^-1, respectively. MBC values of two extracts against three standard strains were 25, 50, 100mg·mL^-1 and 50, 100, 200 mg·mL^-1, MBC values of three drug-resistant bacteria were 100, 100, 100mg·mL^-1 and 200, 200, 200 mg·mL^-1, respectively.展开更多
Seven fractions were isolated from the ethyl acetate extract of Prinsepia utilis Royle by silica gel column chromatography. The antibacterial activities determined by disc diffusion method in vitro, indicated that the...Seven fractions were isolated from the ethyl acetate extract of Prinsepia utilis Royle by silica gel column chromatography. The antibacterial activities determined by disc diffusion method in vitro, indicated that the first and fourth fractions showed better antibacterial activity than the other fractions, while the sixth and seventh fractions did not showed any antibacterial activity. The diameters of the inhibition zone of first and fourth fractions were greater than 10 mm against three standard strains (Staphylococcus aureus ATCC25923, Escherichia coli ATCC44102 and Salmonella sp. CMCC(B)50041) at the concentration of 20 mg/ml. The first fraction was then repeatedly recrystallized in acetone to yield a white snowflake-like compound A, the inhibition zones of which were 14.03 mm, 11.54 mm and 12 mm, respectively. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were measured by broth dilution method at the concentrations ranging from 20 to 0.313 mg/mL. The MIC and MBC values of the first, fourth fractions and compound A were lower than that of oregano oil (positive control) against S. aureus ATCC25923.展开更多
To research the hair-care efficacy of prinsepia utilis oil.We evaluated the haircare efficacies of prinsepia utilis oil through methods such as differential scanning calorimetry(DSC),measuring difficulty level and mec...To research the hair-care efficacy of prinsepia utilis oil.We evaluated the haircare efficacies of prinsepia utilis oil through methods such as differential scanning calorimetry(DSC),measuring difficulty level and mechanical resistance against combing of wet and dry hair,scanning electron microscopy(SEM).According to the analysis of differential scanning calorimetry(DSC),the testing sample has repairing effect on the hairα-keratin damage induced by heated blowing.According to the pictures of scanning electron microscopy(SEM),The test results indicate the repairing or resisting thermal damage effects induced by hair drier of prinsepia utilis oil.By comparison with the blank control group,the strength and work of dry comb and wet comb were significantly reduced in the samples tested with 2%prinsepia utilis oil concentration(P﹤0.05).This indicates the testing sample has the efficacy to improve the easiness of combing and anti-tangle.And friction work is significantly reduced This indicates the smoothing efficacy of the testing sample.and the hair scales status is better;this indicates hair scales repairing efficacy of testing sample.Conclusion:PRINSEPIA UTILIS OIL has the effect of repairing hair scales,resisting thermal damage caused by hair dryer,easy to comb,anti-tangle,smoothing.展开更多
目的建立蕤仁Prinsepiae nux饮片多指标定量检测方法,结合化学模式识别及CRITIC(criteria importance though intercrieria correlation)-逼近理想解排序(technique for order preferenceby similarity to ideal solution,TOPSIS)法评...目的建立蕤仁Prinsepiae nux饮片多指标定量检测方法,结合化学模式识别及CRITIC(criteria importance though intercrieria correlation)-逼近理想解排序(technique for order preferenceby similarity to ideal solution,TOPSIS)法评价不同产地蕤仁饮片质量。方法采用高效液色谱法,以槲皮素、β-谷甾醇为内参物,分别计算没食子酸、原儿茶酸、香草酸、山柰酚与槲皮素,熊果酸、齐墩果酸、委陵菜酸、胡萝卜苷与β-谷甾醇的相对校正因子,对蕤仁饮片中10个成分进行定量检测;同时检测醇溶性浸出物、总灰分和酸不溶性灰分含量。采用化学模式识别以及CRITIC-TOPSIS法评价不同产地蕤仁饮片质量。结果外标法方法学验证结果良好;各相对校正因子重复性良好;外标法与一测多评(quantitative analysis of multicomponents by single marker,QAMS)法计算结果无明显差异(P>0.05)。主成分分析(principal component analysis,PCA)结果显示不同产地16批蕤仁饮片分成3类;正交偏最小二乘法-判别分析(orthogonal partial least squares discriminant analysis,OPLS-DA)结果显示没食子酸、槲皮素、β-谷甾醇、熊果酸、委陵菜酸、齐墩果酸和山柰酚变量重要性投影值均>1,是区分不同产地蕤仁饮片质量的差异性标志物;CRITIC-TOPSIS分析结果显示,法16批蕤仁饮片综合质量评价指标的相对贴近度为0.4072~0.5935,其中陕西、甘肃和山西产蕤仁饮片排名靠前,样品质量较优。结论通过建立蕤仁饮片中多指标定量,结合化学模式识别及CRITIC-TOPSIS分析评价了不同产地蕤仁饮片质量,为蕤仁饮片质量标准的研究及其质量标志物的研究提供参考。展开更多
文摘A new hemiterpenoid, （＋）-（2R,3S）-2-chloro-3-hydroxy-3-methyl-T-buty-rolactone was isolatexi from the leaves of Prinsepia utilis Royle. Its structure was clucidatexi by spectroscopic methods and X-ray crystallographic analysis.
基金Supported by Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT0848)
文摘The purpose of this study is to isolate the active fraction from the crude extract of Prinsepia utilis Royle and to determine its antibacterial activity against the standard and drug-resistant bacteria strains in vitro. The Prinsepia utilis Royle was extracted in three portions by using petroleum ether, ethanol and water by the continuation recirculate extraction. The antibacterial activity of the extracts were evaluated by the determination of Minimum Inhibitory Concentration （MIC） and Minimum Bactericidal Concentration （MBC） values. The results showed that the ethanol extract and aqueous extract of petroleum ether showed the weakest inhibition. MIC values of the aqueous Prinsepia utilis Royle were found that they had significant effects against bacteria, while ethanol extracts against three standard bacteria （S. aureus ATCC25923, E. coli 44102, Salmonella 50041） were 25, 25, 50 mg·mL^-1 and 50, 100, 100 mg·mL^-1, MIC values of three drug-resistant bacteria （S. aureus GL17, E. coli EYAC08-56, Salmonella STQD2G.） were 50, 50, 50 mg·mL^-1 and 100, 100, 200 mg·mL^-1, respectively. MBC values of two extracts against three standard strains were 25, 50, 100mg·mL^-1 and 50, 100, 200 mg·mL^-1, MBC values of three drug-resistant bacteria were 100, 100, 100mg·mL^-1 and 200, 200, 200 mg·mL^-1, respectively.
文摘Seven fractions were isolated from the ethyl acetate extract of Prinsepia utilis Royle by silica gel column chromatography. The antibacterial activities determined by disc diffusion method in vitro, indicated that the first and fourth fractions showed better antibacterial activity than the other fractions, while the sixth and seventh fractions did not showed any antibacterial activity. The diameters of the inhibition zone of first and fourth fractions were greater than 10 mm against three standard strains (Staphylococcus aureus ATCC25923, Escherichia coli ATCC44102 and Salmonella sp. CMCC(B)50041) at the concentration of 20 mg/ml. The first fraction was then repeatedly recrystallized in acetone to yield a white snowflake-like compound A, the inhibition zones of which were 14.03 mm, 11.54 mm and 12 mm, respectively. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were measured by broth dilution method at the concentrations ranging from 20 to 0.313 mg/mL. The MIC and MBC values of the first, fourth fractions and compound A were lower than that of oregano oil (positive control) against S. aureus ATCC25923.
文摘To research the hair-care efficacy of prinsepia utilis oil.We evaluated the haircare efficacies of prinsepia utilis oil through methods such as differential scanning calorimetry(DSC),measuring difficulty level and mechanical resistance against combing of wet and dry hair,scanning electron microscopy(SEM).According to the analysis of differential scanning calorimetry(DSC),the testing sample has repairing effect on the hairα-keratin damage induced by heated blowing.According to the pictures of scanning electron microscopy(SEM),The test results indicate the repairing or resisting thermal damage effects induced by hair drier of prinsepia utilis oil.By comparison with the blank control group,the strength and work of dry comb and wet comb were significantly reduced in the samples tested with 2%prinsepia utilis oil concentration(P﹤0.05).This indicates the testing sample has the efficacy to improve the easiness of combing and anti-tangle.And friction work is significantly reduced This indicates the smoothing efficacy of the testing sample.and the hair scales status is better;this indicates hair scales repairing efficacy of testing sample.Conclusion:PRINSEPIA UTILIS OIL has the effect of repairing hair scales,resisting thermal damage caused by hair dryer,easy to comb,anti-tangle,smoothing.
文摘目的建立蕤仁Prinsepiae nux饮片多指标定量检测方法,结合化学模式识别及CRITIC(criteria importance though intercrieria correlation)-逼近理想解排序(technique for order preferenceby similarity to ideal solution,TOPSIS)法评价不同产地蕤仁饮片质量。方法采用高效液色谱法,以槲皮素、β-谷甾醇为内参物,分别计算没食子酸、原儿茶酸、香草酸、山柰酚与槲皮素,熊果酸、齐墩果酸、委陵菜酸、胡萝卜苷与β-谷甾醇的相对校正因子,对蕤仁饮片中10个成分进行定量检测;同时检测醇溶性浸出物、总灰分和酸不溶性灰分含量。采用化学模式识别以及CRITIC-TOPSIS法评价不同产地蕤仁饮片质量。结果外标法方法学验证结果良好;各相对校正因子重复性良好;外标法与一测多评(quantitative analysis of multicomponents by single marker,QAMS)法计算结果无明显差异(P>0.05)。主成分分析(principal component analysis,PCA)结果显示不同产地16批蕤仁饮片分成3类;正交偏最小二乘法-判别分析(orthogonal partial least squares discriminant analysis,OPLS-DA)结果显示没食子酸、槲皮素、β-谷甾醇、熊果酸、委陵菜酸、齐墩果酸和山柰酚变量重要性投影值均>1,是区分不同产地蕤仁饮片质量的差异性标志物;CRITIC-TOPSIS分析结果显示,法16批蕤仁饮片综合质量评价指标的相对贴近度为0.4072~0.5935,其中陕西、甘肃和山西产蕤仁饮片排名靠前,样品质量较优。结论通过建立蕤仁饮片中多指标定量,结合化学模式识别及CRITIC-TOPSIS分析评价了不同产地蕤仁饮片质量,为蕤仁饮片质量标准的研究及其质量标志物的研究提供参考。
