摘要
为探究青刺尖(Prinsepia utilis Royle)主要抗氧化活性成分,阐明其药效物质基础,本研究以1,1-二苯基-2-三硝基苯肼自由基(1,1-diphenyl-2-picryl-hydrazyl radical,DPPH·)清除率为指标,采用高效液相色谱(high performance liquid chromatography,HPLC)建立11批青刺尖提取物指纹图谱,并结合皮尔逊、斯皮尔曼和偏最小二乘回归分析探讨了指纹图谱共有峰与抗氧化作用间的谱-效关系,筛选出对抗氧化活性贡献较大的组分进行质谱定性和高效液相色谱定量分析,进一步通过体外DPPH·、ABTS^(+)·、·OH清除能力和人皮肤成纤维细胞(human foreskin fibroblasts,HFF)抗衰活性检测,评价其抗氧化及抗衰活性。结果从11批青刺尖提取物指纹图谱中标定13个色谱峰,并识别出其中6个化学成分,其中芦丁、水仙苷、碟豆素、夏佛塔苷和香蒲新苷等化合物为青刺尖发挥抗氧化功效的潜在物质成分,定量分析结果表明各化学成分的含量存在一定差异,碟豆素含量最高(738.123μg/mL),其次是芦丁(538.612μg/mL)、水仙苷(385.881μg/mL)和香蒲新苷(337.168μg/mL),且各单体化合物均表现出较好的自由基清除活性和良好的抗衰效果,其中芦丁和水仙苷能有效清除DPPH·、ABTS^(+)·及·OH,其中芦丁对DPPH·和ABTS^(+)·的清除率分别为92.84%和92.44%,对·OH清除作用的IC_(50)值为0.45 mg/mL;水仙苷对DPPH·和ABTS^(+)·的清除率为37.71%和82.01%,·OH清除作用的IC_(50)值为1.01 mg/mL。夏佛塔苷、香蒲新苷(P<0.01)及芦丁(P<0.05)可显著抑制SA-β-gal活性,降低衰老相关β-半乳糖苷酶阳性细胞比例,显示出良好的抗衰效果;细胞氧化应激模型中,香蒲新苷、蝶豆素、水仙苷及夏佛塔苷、芦丁能显著下调MMP-1、MMP-3和MMP-9的表达(P<0.001),促进皮肤胶原蛋白合成并抑制其降解,具有潜在的抗衰老能力。综上表明青刺尖的抗氧化功效可能是由夏佛塔苷、香蒲新苷、芦丁、水仙苷及碟豆素等多种化学成分共同作用产生的结果。
To investigate the antioxidant chemical constituents of Prinsepia utilis Royle and reveal its pharmacodynamic substance basis.HPLC was used to establish the fingerprints of different batches of Prinsepia utilis Royle extracts(PRE).The DPPH scavenging activity was used as an indicator of drug efficacy.Pearson correlation,Spearman analysis,and partial least squares regression(PLSR)analysis were used to study the spectral efficiency relationship between the common peaks of the HPLC fingerprint spectra and the antioxidant efficacy substances of PRE.This was followed by LC-MS and HPLC analyses to identify the primary antioxidant substances.We assessed the antioxidant and anti-aging effectiveness of these active substances through various methods:scavenging 1,1-diphenyl-2-picrylhydrazyl radicals(DPPH·),2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid radicals)(ABTS^(+)·),OH radicals(·OH),and anti-aging activity in HFF cells.Results showed that thirteen common peaks were identified and six chemical components were identified from the fingerprints of 11 batches of PRE.Among them,rutin,narcissoside,clitorin,shaftoside and typhaneoside may be potential antioxidant substances in the 11 extracts of PRE,and the contents of the above five compounds exhibited significant differences.In which,the content of clitorin was the highest(738.123μg/mL),followed by rutin(538.612μg/mL),narcissoside(385.881μg/mL),and typhaneoside(337.168μg/mL).Rutin and narcissoside demonstrates significant efficacy in scavenging DPPH·,ABTS^(+)·,and·OH radicals,with the scavenging rates and IC_(50) values recorded at DPPH·92.84%(Rutin)and 37.71%(narcissoside),ABTS^(+)·92.44%(Rutin)and 82.01%(narcissoside),·OH 0.45 mg/mL(Rutin)and 1.01 mg/mL(narcissoside),respectively.Additionally,schaftoside,typhaneoside,and rutin significantly inhibited SA-β-gal activity,reduced the proportion of senescence-associatedβ-galactosidase-positive cells,and exhibits notable anti-aging effects(P<0.01).In the hydrogen peroxide(H2O2)-induced cellular oxidative stress model,typhaneoside,clitorin,narcissoside,and shaftoside,along with rutin significantly downregulated the expression of MMP-1,MMP-3,and MMP-9(P<0.001),promoted skin collagen synthesis,and inhibited its degradation,demonstrating potential anti-aging capabilities.This altogether indicates that the antioxidant effect of Prinsepia utilis Royle is the result of the combined effect of multiple chemical components such as shaftoside,typhaneoside,rutin,narcissoside and clitorin.
作者
桂鹏燕
杨秀霞
杨兆祥
曲丽萍
GUI Pengyan;YANG Xiuxia;YANG Zhaoxiang;QU Liping(Yunnan Characteristic Plant Extraction Laboratory Co.,Ltd.,Kunming 650106,China;Yunnan Botanee Bio-technology Group Co.,Ltd.,Kunming 650106,China)
出处
《食品工业科技》
北大核心
2026年第4期348-359,共12页
Science and Technology of Food Industry
基金
云南特色植物提取实验室自主研究项目(2022YKZY001,2023YKZY004)。
关键词
青刺尖
抗氧化
谱-效关系
Pearson
相关性
偏最小二乘回归
Prinsepia utilis Royle
antioxidative
spectrum-effect relationship
Pearson correlation
partial least squares regression
