Effects of pretreatment of mouse with low doses of internally deposited147Pm on the mutagenicity of both the bone marrow lymphocytes and the spermatoblasts induced by subsequent high dose of internally deposited 147Pm...Effects of pretreatment of mouse with low doses of internally deposited147Pm on the mutagenicity of both the bone marrow lymphocytes and the spermatoblasts induced by subsequent high dose of internally deposited 147Pm were studied.Kunndng male mice were injected from the tail vein with low doses of "’Pm(NO.).Of 0.37, 3.7 and 37Bq/g body weight. Three dabs later the mice were injected witha high dose of 147Pm(NO.)3 of 18.5 kBq/g body weight. The animals were sacrificedat 24h after the high dose injection. The results show that the bone marrow lymphocytes and the spermatoblasts pre-exposed in fido to low doses of 147Pm can producean adaptive response. The radioadapted cells had a resistance to the mutagenicity induced by the subsequent high dose of 14’Pm, that is, the proportion of both the bonemarrow lymphocytes containing ndcronuclei and the sperm shape abnormalities wassignificantly lower than those for the pure high dose group (P <0.01). In addition, theinduction of the adaptive response of the cells possesses the different dose ranges forthe different biological parameters. The 147Pm dose ranges of the adaptive responseare 0.37-3.7 and 0.37-37 Bq/g body weight for the frequency of micronucleated bonemarrow lymphocytes and abnormal sperrnst respectively.展开更多
The early risk of internal contaminated accumulation of 147Pm is in blood cells and endothelial cells, especially in red blood cells. Then 147Pm is selectively deposited in ultrastructure of liver cells, such as in nu...The early risk of internal contaminated accumulation of 147Pm is in blood cells and endothelial cells, especially in red blood cells. Then 147Pm is selectively deposited in ultrastructure of liver cells, such as in nucleus, nucleolus, rough endoplasmic reticulum, mitochondria and microbodies. Dense tracks also appear in mitochondria and lysosome of pedal cells within renal corpuscle, and so does in nucleus as well as in mitochondria and microbodies of epicyte of kidney near-convoluted tubule. With the prolongation of observing time, 147Pm is selectively and steadily deposited in subcellular level of organic component for bone. Substantial amount of 147Pm is taken up into the nuclear fraction of osteoclasts and osteoblasts. Particularly, in organelles 147Pm is mainly accumulated in rough endoplasmic reticulum and in mitochondria.Autoradiographic tracks especially localize in combined point between Golgi complex and transitive vesicle of rough endoplasmic reticulum. In addition, numerous 147Pm deposited in collagenous fibre within interstitial of bone cells is hardly excreted.展开更多
文摘Effects of pretreatment of mouse with low doses of internally deposited147Pm on the mutagenicity of both the bone marrow lymphocytes and the spermatoblasts induced by subsequent high dose of internally deposited 147Pm were studied.Kunndng male mice were injected from the tail vein with low doses of "’Pm(NO.).Of 0.37, 3.7 and 37Bq/g body weight. Three dabs later the mice were injected witha high dose of 147Pm(NO.)3 of 18.5 kBq/g body weight. The animals were sacrificedat 24h after the high dose injection. The results show that the bone marrow lymphocytes and the spermatoblasts pre-exposed in fido to low doses of 147Pm can producean adaptive response. The radioadapted cells had a resistance to the mutagenicity induced by the subsequent high dose of 14’Pm, that is, the proportion of both the bonemarrow lymphocytes containing ndcronuclei and the sperm shape abnormalities wassignificantly lower than those for the pure high dose group (P <0.01). In addition, theinduction of the adaptive response of the cells possesses the different dose ranges forthe different biological parameters. The 147Pm dose ranges of the adaptive responseare 0.37-3.7 and 0.37-37 Bq/g body weight for the frequency of micronucleated bonemarrow lymphocytes and abnormal sperrnst respectively.
文摘The early risk of internal contaminated accumulation of 147Pm is in blood cells and endothelial cells, especially in red blood cells. Then 147Pm is selectively deposited in ultrastructure of liver cells, such as in nucleus, nucleolus, rough endoplasmic reticulum, mitochondria and microbodies. Dense tracks also appear in mitochondria and lysosome of pedal cells within renal corpuscle, and so does in nucleus as well as in mitochondria and microbodies of epicyte of kidney near-convoluted tubule. With the prolongation of observing time, 147Pm is selectively and steadily deposited in subcellular level of organic component for bone. Substantial amount of 147Pm is taken up into the nuclear fraction of osteoclasts and osteoblasts. Particularly, in organelles 147Pm is mainly accumulated in rough endoplasmic reticulum and in mitochondria.Autoradiographic tracks especially localize in combined point between Golgi complex and transitive vesicle of rough endoplasmic reticulum. In addition, numerous 147Pm deposited in collagenous fibre within interstitial of bone cells is hardly excreted.
