摘要
木文用电镜放射自显影术研究了重核裂片^(147)Pm在体内亚细胞水平滞留动态。发现^(147)Pm的内污染沉积早期在血细胞和血管内皮细胞中,尤以在红细胞的沉积为多。^(147)Pm呈选择性滞留于肝细胞亚微结构中,可较多的掺入枯否细胞、肝细胞核和核仁内,在胞质中的糙面内质网、线粒体、微体和糖元中亦有滞留,且主要沉积于糙面内质网和线粒体,在高尔基复合体与糙面内质网所芽生的转移小泡融合处,亦有^(147)Pm滞留的放射自显影象。而在肾小体足细胞的线粒体和溶酶体中,有浓集的放射自显影径迹呈现。在肾近端小管曲部上皮细胞核中和胞质的线粒体和微体中,亦有较多的^(147)Pm滞留。 随着观察时间的延续,^(147)Pm在骨有机质亚微结构中呈现选择性、持续性的沉积增多。可浓集于破骨细胞和成骨细胞核中。此外在骨组织细胞间质中的胶原纤维部位,亦有较多的^(147)Pm沉积,难以排除。
This study investigated the subcellular localization of radionuclide
^(147)Pm in tissue cells by electron microscopic autoradiography. The early risk of inter-
nal contaminated accumulation of ^(147)Pm was in blood cells and endothelial cells, espe-
cially in red blood cells where more numerous of autoradiographic tracks appeared. Then
^(147)Pm was selectively deposited in ultrastructure of liver cells, such as in nucleus,
nucleous, rough endoplasmic reticulum, mitochondria and microbodies. In organelles
^(147)Pm was mainly accumulated in rough endoplasmic reticulum and mitochondria. Au-
toradiographic tracks especially localized in combined point between Golgi complex and
transitive vesicle of rough endoplasmic reticulum. Autoradiographic study also demon-
strated, where dense tracks appeared in mitochondria and lysosome of podal cells within
renal corpuscle. In nucleus as well as in mitochondria and microbodies within epicyte
of kidney near-convoluted tubule where also more numerous tracks of ^(147)Pm accumula-
ted. With the protongation of observing time, ^(147)Pm was selectively and steadily depo-
sited in subcellular level of organic component bone. Electron microscopic autoradiogra-
phy showed, that substential amounts of ^(147)Pm was taken up into the nuclear fraction
of osteoclasts and osteoblasts. In addition, where more numerous tracks of ^(147)Pm was
deposited in collagenous fibre within interstitial of bone cells and was hardly excreted.
出处
《辐射研究与辐射工艺学报》
CAS
CSCD
北大核心
1992年第1期12-19,共8页
Journal of Radiation Research and Radiation Processing
基金
国家自然科学基金
关键词
Pm147
核裂片
组织细胞
污染
机体
Electron microscopic autoradiography
Accumulation
^(147)Pm
Subcellu1ar level
