Platycodin D(PD), a triterpenoid saponin isolated from Platycodonis Radix, is a famous Chinese herbal medicine that has been shown to have anti-proliferative effects in several cancer cell lines. The aim of this study...Platycodin D(PD), a triterpenoid saponin isolated from Platycodonis Radix, is a famous Chinese herbal medicine that has been shown to have anti-proliferative effects in several cancer cell lines. The aim of this study was to determine the changes in cellular proteins after the treatment of hepatocellular carcinoma HepG2 cells with PD using proteomics approaches. The cell viability was determined using the MTT assay. The proteome was analyzed by two-dimensional difference gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Western blot analysis was used to confirm the expression of changed proteins. Our results showed that PD inhibited the proliferation of HepG2 cells in concentration- and time-dependent manners. Sixteen proteins were identified to be up-regulated in PD-treated HepG2 cells, including ATP5 H, OXCT1, KRT9, CCDC40, ERP29, RCN1, ZNF175, HNRNPH1, HSP27, PA2G4, PHB, BANF1, TPM3, ECH1, LGALS1, and MYL6. Three proteins(i.e., RPS12, EMG1, and KRT1) decreased in HepG2 cells after treatment with PD. The changes in HSP27 and PHB were further confirmed by Western blotting. In conclusion, our results shed new lights on the mechanisms of action for the anti-cancer activity of PD.展开更多
Objective:To study the transmembrane transport of chemical components of Chinese herbs and to explore the function of platycodin D (PD) on biomembranes.Methods:Interaction between PD and the dipalmitoylphosphatidylcho...Objective:To study the transmembrane transport of chemical components of Chinese herbs and to explore the function of platycodin D (PD) on biomembranes.Methods:Interaction between PD and the dipalmitoylphosphatidylcholine (DPPC) bilayer was reproduced by molecular dynamics simulation with the Martini force field.A model validation and methodological study were first performed,and were based on simulation investigations of transmembrane transport for three herbal compounds with distinct hydrophilic properties.Results:PD increased the mobility of the DPPC bilayer since its aglycone strongly interacted with the hydrophobic layer,which broke the structure of the gate layer,and weakened the ordered performance of hydrophobic tails.Conclusion:The Martini force field was successfully applied to the study of the interaction between herbal compounds and a biological membrane.By combining the dynamics equilibrium morphology,the distribution of drugs inside and outside the biomembrane,and the interaction sites of drugs on the DPPC bilayer,factors influencing transmembrane transport of drugs were elucidated and the function of platycodin D in a biological membrane was reproduced.展开更多
目的 研究传统加工与趁鲜加工桔梗生饮片与蜜炙饮片成分的变化,为桔梗产地加工炮制一体化工艺提供科学依据。方法 用传统方法和趁鲜切制法对桔梗进行加工,制成传统饮片、鲜切饮片、传统蜜炙饮片、鲜切蜜炙饮片。将桔梗皂苷D、总皂苷、...目的 研究传统加工与趁鲜加工桔梗生饮片与蜜炙饮片成分的变化,为桔梗产地加工炮制一体化工艺提供科学依据。方法 用传统方法和趁鲜切制法对桔梗进行加工,制成传统饮片、鲜切饮片、传统蜜炙饮片、鲜切蜜炙饮片。将桔梗皂苷D、总皂苷、醇浸出物、总多糖的含量作为考察指标,采用熵权法、灰色关联度法和优劣解距离法(technique for order preference by similarity to ideal solution,TOPSIS)综合分析不同加工方法对桔梗质量的影响。结果 鲜切饮片桔梗皂苷D、总皂苷、浸出物、总多糖含量大于传统饮片。桔梗生品饮片的指标权重从高到低依次是桔梗皂苷D、醇浸出物、总皂苷、总多糖,蜜炙桔梗的指标权重从高到低依次是总多糖、桔梗皂苷D、总皂苷、醇浸出物。鲜切饮片的相对关联度和相对贴合度都高于传统饮片,表明桔梗鲜切加工方式得到的饮片质量更优。结论 产地加工炮制一体化可以提高桔梗饮片质量,减少有效成分的流失,缩短加工时长。展开更多
目的:建立测定桔梗样品中桔梗皂苷 E 含量的方法。方法:采用 HPLC-ELSD 法,C_(18)柱(3.9mm×300mm,4μm),以乙腈-水为流动相梯度洗脱,流速1.0mL·min^(-1),ELSD 漂移管温度112℃,载气(N_2)流速3.2L·min^(-1)。结果:不同产...目的:建立测定桔梗样品中桔梗皂苷 E 含量的方法。方法:采用 HPLC-ELSD 法,C_(18)柱(3.9mm×300mm,4μm),以乙腈-水为流动相梯度洗脱,流速1.0mL·min^(-1),ELSD 漂移管温度112℃,载气(N_2)流速3.2L·min^(-1)。结果:不同产地的桔梗中均含有桔梗皂苷 E,但含量差别较大,其中以安徽青阳产含量最高(0.26%)。结论:本方法稳定、可靠,桔梗皂苷 E 可作为评价桔梗质量的指标之一。展开更多
基金supported by the Science and Technology Development Fund,Macao S.A.R(FDCT)(070/2013/A)the Research Fund of University of Macao(SRG026-ICMS13-LJJ and MRG008-LJJ2014-ICMS)+1 种基金the Administration of Traditional Chinese Medicine of Zhejiang Province(No.2012 ZA028)Program of Xinmiao Talents in Zhejiang Province(No.2010 R410024)
文摘Platycodin D(PD), a triterpenoid saponin isolated from Platycodonis Radix, is a famous Chinese herbal medicine that has been shown to have anti-proliferative effects in several cancer cell lines. The aim of this study was to determine the changes in cellular proteins after the treatment of hepatocellular carcinoma HepG2 cells with PD using proteomics approaches. The cell viability was determined using the MTT assay. The proteome was analyzed by two-dimensional difference gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Western blot analysis was used to confirm the expression of changed proteins. Our results showed that PD inhibited the proliferation of HepG2 cells in concentration- and time-dependent manners. Sixteen proteins were identified to be up-regulated in PD-treated HepG2 cells, including ATP5 H, OXCT1, KRT9, CCDC40, ERP29, RCN1, ZNF175, HNRNPH1, HSP27, PA2G4, PHB, BANF1, TPM3, ECH1, LGALS1, and MYL6. Three proteins(i.e., RPS12, EMG1, and KRT1) decreased in HepG2 cells after treatment with PD. The changes in HSP27 and PHB were further confirmed by Western blotting. In conclusion, our results shed new lights on the mechanisms of action for the anti-cancer activity of PD.
基金The experiment was financially supported by the National Natural Science Foundation of China(81473364)Beijing Natural Science Foundation(7162122)Excellent Talents Training Subsidy Scheme of Beijing(2013D009999000003).
文摘Objective:To study the transmembrane transport of chemical components of Chinese herbs and to explore the function of platycodin D (PD) on biomembranes.Methods:Interaction between PD and the dipalmitoylphosphatidylcholine (DPPC) bilayer was reproduced by molecular dynamics simulation with the Martini force field.A model validation and methodological study were first performed,and were based on simulation investigations of transmembrane transport for three herbal compounds with distinct hydrophilic properties.Results:PD increased the mobility of the DPPC bilayer since its aglycone strongly interacted with the hydrophobic layer,which broke the structure of the gate layer,and weakened the ordered performance of hydrophobic tails.Conclusion:The Martini force field was successfully applied to the study of the interaction between herbal compounds and a biological membrane.By combining the dynamics equilibrium morphology,the distribution of drugs inside and outside the biomembrane,and the interaction sites of drugs on the DPPC bilayer,factors influencing transmembrane transport of drugs were elucidated and the function of platycodin D in a biological membrane was reproduced.
文摘目的 研究传统加工与趁鲜加工桔梗生饮片与蜜炙饮片成分的变化,为桔梗产地加工炮制一体化工艺提供科学依据。方法 用传统方法和趁鲜切制法对桔梗进行加工,制成传统饮片、鲜切饮片、传统蜜炙饮片、鲜切蜜炙饮片。将桔梗皂苷D、总皂苷、醇浸出物、总多糖的含量作为考察指标,采用熵权法、灰色关联度法和优劣解距离法(technique for order preference by similarity to ideal solution,TOPSIS)综合分析不同加工方法对桔梗质量的影响。结果 鲜切饮片桔梗皂苷D、总皂苷、浸出物、总多糖含量大于传统饮片。桔梗生品饮片的指标权重从高到低依次是桔梗皂苷D、醇浸出物、总皂苷、总多糖,蜜炙桔梗的指标权重从高到低依次是总多糖、桔梗皂苷D、总皂苷、醇浸出物。鲜切饮片的相对关联度和相对贴合度都高于传统饮片,表明桔梗鲜切加工方式得到的饮片质量更优。结论 产地加工炮制一体化可以提高桔梗饮片质量,减少有效成分的流失,缩短加工时长。
文摘目的:建立测定桔梗样品中桔梗皂苷 E 含量的方法。方法:采用 HPLC-ELSD 法,C_(18)柱(3.9mm×300mm,4μm),以乙腈-水为流动相梯度洗脱,流速1.0mL·min^(-1),ELSD 漂移管温度112℃,载气(N_2)流速3.2L·min^(-1)。结果:不同产地的桔梗中均含有桔梗皂苷 E,但含量差别较大,其中以安徽青阳产含量最高(0.26%)。结论:本方法稳定、可靠,桔梗皂苷 E 可作为评价桔梗质量的指标之一。
