Two phenylethanoid glycosides（PhGs）, plantamajoside and acteoside, were isolated and purified from the aerial parts ofPlantago asiatica for the first time by high performance centrifugal partition chromatography（HP...Two phenylethanoid glycosides（PhGs）, plantamajoside and acteoside, were isolated and purified from the aerial parts ofPlantago asiatica for the first time by high performance centrifugal partition chromatography（HPCPC） with ethyl acetate, n-butanol, ethanol and water（0.5：0.5：0.1：1, volume ratio） as solvent system. A total of 45.6 mg of plantamajoside and 293.8 mg of acteoside were purified from 1341 mg of the n-butanol extract of P. asiatica, with a purity of 〉93.3% as determined by HPLC. The HPCPC fractions were analyzed by HPLC-DAD and the structures were identified by their retention time, UV, electrospray ionization multi stage tandem mass spectrometry（ESI-MSn） in the negative ion mode, and confirmed by NMR experiments. The characteristic fragment ions of ESI-MS of the two PhGs isolated from Plantago asiatica were discussed, which are specific and useful for the identification of the structures of PhGs.展开更多
Objective:This study aimed to elucidate the active components and mechanisms of Plantago asiatica L.(PAL)in the treatment of gout.Materials and Methods:Based on its polarity,PAL was partitioned into three parts,P1,P2,...Objective:This study aimed to elucidate the active components and mechanisms of Plantago asiatica L.(PAL)in the treatment of gout.Materials and Methods:Based on its polarity,PAL was partitioned into three parts,P1,P2,and P3,through gradient elution using AB-8 macroporous resin columns.The primary constituents of these fractions include iridoids,phenylethanoid glucosides,and flavonoids.Subsequently,a comprehensive in vitro and in vivo screening and evaluation model was developed considering the fundamental pathological processes of gout,including the inhibition of uric acid(UA)production,augmentation of UA excretion,and anti-inflammatory effects.This model was used to analyze the anti-gout activity of P1,P2,P3,and the overall extracts derived from PAL.Results:All the PAL parts exhibited specific therapeutic properties as part of gout therapy.Specifically,the P2 part demonstrated the ability to diminish interleukin-1 beta levels,lower UA concentrations,suppress joint inflammation in rats,and restore abnormal parameters associated with gout,such as creatinine and urea nitrogen.These findings highlight the significant effect of P2 on gout treatment.Conclusions:Following a thorough assessment of the outcomes derived from diverse anti-gout experiments,P2 exhibited superior anti-gout properties compared to the remaining parts.P2 demonstrated a restorative effect on the aberrant parameters associated with gout,thereby comprehensively elucidating the therapeutic mechanism of P2 against gout.This study provides substantial evidence and serves as a reference for future investigations regarding the material foundation of PAL in gout treatment.展开更多
Two phenylethanoid glycosides （PhGs） were isolated and purified from the aerial parts of Plantago asiatica for the first time by high performance centrifugal partition chromatography （HPCPC） using ethyl acetate-n-...Two phenylethanoid glycosides （PhGs） were isolated and purified from the aerial parts of Plantago asiatica for the first time by high performance centrifugal partition chromatography （HPCPC） using ethyl acetate-n-butanol-ethanol-water （0.5：0.5：0.1：1, v/v/v/v）. A total of 45.6 mg of compound 1 and 293.8 mg of compound 2 were purified from 1341 mg of the n-butanol extract of P. asiatica. The structures of the two PhGs were tentatively identified as plantamajoside and aeteoside or isoacteoside by eleetrospray ionization multi stage tandem mass spectrometry （ESI-MS^n） in the negative ion mode.展开更多
Box dimension and information dimension based on individual and leaf number were applied to analyze the distribution patterns of Plantato asiatica and P. viriginica in suburbs of Jinhua. The results showed that both t...Box dimension and information dimension based on individual and leaf number were applied to analyze the distribution patterns of Plantato asiatica and P. viriginica in suburbs of Jinhua. The results showed that both the box dimension and the information dimention of P. virginica were obviously higher than those of P. asiatica in the high human-distributed environment. P. asiatica was mostly distributed in the environment with compacted soil. The information dimension on the basis of leaf probability in the boxes showed that the populations of P. virginica and P. asiatica in low density could exploit environmental resources effectively.展开更多
Four new indole alkaloids,plasiaticines A-D(1-4),together with two known ones,were isolated from the seeds of Plantago asiatica.The structures of the new compounds were elucidated on the basis of comprehensive analysi...Four new indole alkaloids,plasiaticines A-D(1-4),together with two known ones,were isolated from the seeds of Plantago asiatica.The structures of the new compounds were elucidated on the basis of comprehensive analysis of spectroscopic data.All compounds were tested for their cytotoxic activity,and all compounds except 4 were tested for their acetylcholinesterase(AChE)inhibitory activities.展开更多
The present study aimed at evaluation of prophylactic efficacy and possible mechanisms of asiaticoside （AS） based standardized extract of Centella asiatica （L.） Urban leaves （INDCA） in animal models of migraine....The present study aimed at evaluation of prophylactic efficacy and possible mechanisms of asiaticoside （AS） based standardized extract of Centella asiatica （L.） Urban leaves （INDCA） in animal models of migraine. The effects of oral and intranasal （i.n.） pretreatment of INDCA （acute and 7-days subacute） were evaluated against nitroglycerine （NTG, 10 mg·kg^-1, i.p.） and bradykinin （BK, 10 μg, intra-arterial） induced hyperalgesia in rats. Tail flick latencies （from 0 to 240 rain） post-NTG treatment and the number of vocalizations post-BK treatment were recorded as a measure of hyperalgesia. Separate groups of rats for negative （Normal） and positive （sumatriptan, 42 mg.kg ^-1, s.c.） controls were included. The interaction of 1NDCA with selective 5-HT1A, 5-HT1B, and 5-HTI D receptor antagonists （NAN-190, Isamoltane hemifumarate, and BRL-15572 respectively） against NTG-induced hyperalgesia was also evaluated. Acute and sub-acute pre-treatment of INDCA [10 and 30 mg.kg^-1 （oral） and 100 μg/rat （i.n.） showed significant anti-nociception activity, and reversal of the NTG-induced hypera｜gesia and brain 5-HT concentration decline. Oral pre-treatment with INDCA （30 mg·kg ^-1, 7 d） showed significant reduction in the number of vocalization. The anti-nociceptive effects of INDCA were blocked by 5-HTIA and 5-HT1B but not 5-HT1D receptor antagonists. In conclusion, 1NDCA demonstrated promising anti-nociceptive effects in animal models of migraine, probably through 5-HT1A/1B medicated action.展开更多
Understanding polysaccharide-gut microbe interactions is critical for elucidating their health benefits for the host.This study aimed to investigate the interaction between polysaccharides from Plantago asiatica L.see...Understanding polysaccharide-gut microbe interactions is critical for elucidating their health benefits for the host.This study aimed to investigate the interaction between polysaccharides from Plantago asiatica L.seeds(PLCP)and Bacteroides by using antibiotics induced pseudo-sterile mice that gavaged with Bacteroides strains for 5 days and then switched to 7 days of PLCP,based on the changes of gut microbiota,short-chain fatty acids(SCFAs)and serum biochemical parameters.Seven Strains from different species with good PLCP utilization ability successfully survived in the gut of these mice.The additional PLCP tended to significantly and differently impact the relative abundance of Bacteroides only in B.thetaiotaomicron C1 and B.salyersiae C13 treated mice(0.05<P<0.1).LEfSe analysis further suggested strain-specific interaction consequence with PLCP on microbiota.Notably,B.koreensis C16 and B.salyersiae C13 significantly elevated total colonic SCFAs levels,respectively,mainly due to the increase in acetic acid,which were not affected by further PLCP treatment.PLCP significantly decreased propionic-and increased butyric acid levels in mice recieving B.koreensis C16,while the former was also seen in B.fragilis C11 and the latter in B.salyersiae C13 treated ones.Moreover,only B.salyersiae C13+PLCP treatment significantly elevated serum total protein,albumin,and HDL-C levels in mice.These findings establish a theoretical framework for elucidating the mechanisms underlying PLCP-Bacteroides in-teractions,providing fundamental insights for future mechanistic studies and therapeutic development targeting gut microbiota.展开更多
Antimicrobial agents such as bavistin, cefotaxime and kanamycin were evaluated for their effects on the rapid shoot regeneration from nodal explants of Centella asiatica (L.). Filter sterilized bavistin (250 mg/L) was...Antimicrobial agents such as bavistin, cefotaxime and kanamycin were evaluated for their effects on the rapid shoot regeneration from nodal explants of Centella asiatica (L.). Filter sterilized bavistin (250 mg/L) was augmented alone and in combination with cytokinins such as BAP and TDZ into the media to trace the effect on regeneration. On this basis, the potential use of bavistin (150 mg/L) along with BAP (2.0 mg/L) was evaluated which showed the maximum shoot number (6.6) and shoot length (4.4 cm) respectively. Cefotaxime at the concentration of 100 μM/L was found to be effective to obtain the maximum shoot number formation (5.8) with the regeneration frequency (90%). Kanamycin at the concentration of 80 μM/L induced maximum shoot regeneration (5.12). Kanamycin at 100 μM/L or at higher concentrations reduced the shoot regeneration. The best rooting response was noticed when in vitro regenerated microshoots were transferred to the rooting medim which was supplemented with IBA (2.0 mg/L). This combination generates 90% of regeneration frequency and maximum number of roots per shoot (14.2) and high root length (4.2 cm). The rooted plants were acclimatized and transferred to field for survivalance. The addition of antibiotics was found to be more effective and safer for using since their effects on regeneration were found to be negligible.展开更多
In this study we designed a novel,cost‐efficient and green method for the synthesis of copper nanoparticles(Cu NPs)supported on manganese dioxide(MnO2)NPs,using Centella asiatica L.leaf extract as a naturally‐source...In this study we designed a novel,cost‐efficient and green method for the synthesis of copper nanoparticles(Cu NPs)supported on manganese dioxide(MnO2)NPs,using Centella asiatica L.leaf extract as a naturally‐sourced reducing agent,without stabilizers or surfactants.This synthetic process is environmentally‐friendly and avoids the use of toxic reducing agents.Phenolic hydroxyl groups in the leaf extract are believed to reduce Cu2+in solution to generate Cu NPs that are subsequently stabilized on the MnO2NP surfaces.The resulting Cu/MnO2nanocomposite was fully characterized using X‐ray diffraction,transmission electron microscopy,field emission scanning electron microscopy,energy‐dispersive X‐ray spectroscopy and Fourier transform infrared spectroscopy.This material was found to function as a highly active,efficient and recyclable heterogeneous catalyst for the reduction of Congo red,rhodamine B and methylene blue as well as nitro compounds such as2,4‐dinitrophenylhydrazine and4‐nitrophenol in the presence of NaBH4in aqueous media at ambient temperature.The high stability of the Cu/MnO2nanocomposite also allows the catalyst to be separated and reused several times without any significant loss of activity.?2018,Dalian Institute of Chemical Physics,Chinese Academy of Sciences.Published by Elsevier B.V.All rights reserved.展开更多
Objective:To investigate the antifertility properties ofCentella asiatica L. ethanolic extract and identify the sperm proteomic changes in treated male rats.Methods: A total of 32 male Sprague-Dawley rats were divided...Objective:To investigate the antifertility properties ofCentella asiatica L. ethanolic extract and identify the sperm proteomic changes in treated male rats.Methods: A total of 32 male Sprague-Dawley rats were divided into two groups: control group (n=16) received distilled water and treatment group (n=16) which received 300 mg/kg ofCentella asiatica L. extract for 42 d. After the treatment period, the number of implantation sites was recorded and the sperm proteomic changes were analysed by 2D gel electrophoresis. In addition, the expression of protein spots was quantified by MALDI-TOF analysis.Results:Centella asiatica L. extract resulted in low number of implantation sites in the treatment group (100.00±2.82) compared to the control group (183.00±2.14). The percentage of infertile male rats in the treatment group was higher (43.75%) compared to the control group (18.75%). Proteomic analysis showed the expression of protein spots identified in the treatment group decreased with 234 spots compared to the control group with 282 spots.Conclusions:The results revealed that there was antifertility activity in the male rats with the administration ofCentella asiatica L. ethanol extract and the identified proteins could provide understanding on the adverse effect ofCentella asiaticain male reproductive system.展开更多
Centella asiatica (L.) is commonly known as Thankuni plant and has ethnobotanical importance in Bangladesh. Present experiment was conceded to investigate the wilt disease of C. asiatica, vegetative growth and molecul...Centella asiatica (L.) is commonly known as Thankuni plant and has ethnobotanical importance in Bangladesh. Present experiment was conceded to investigate the wilt disease of C. asiatica, vegetative growth and molecular characterization of pathogenic fungi. Pathogenic fungus, Fusarium equiseti was identified as a causal agent of wilt disease in C. asiatica. The effect of culture media on the mycelial growth of F. equiseti showed the highest (89.25 mm) on potato dextrose agar (PDA) medium followed by carrot agar (CA) medium and the lowest growth (40.25 mm) was measured in HA medium. The optimal temperature and pH for mycelial growth of F. equiseti were 30°C and 7, respectively. The genetic variation of the selected species of fungi, the internal transcribed spacer (ITS) region was amplified using ITS4 and ITS5 primers and sequenced. The PCR product of the ITS region of F. equiseti was 535 bp. Phylogenetic tree of thirty-seven strains of Fusarium sp. based on the nucleotide sequences of the ITS region using the neighbor-joining method with 1000 bootstrapping indicated that 98% - 100% identity with MN886590.1 JUF0046 (F. equiseti). ITS sequences are generally constant, or show little variation within species, but vary between species in a genus. The ITS region is relatively short and can be easily amplified by PCR using universal single primer pairs. Genetic distance exhibited high level of similarity with identical ITS sequences. To date, no published research articles are found on the molecular identification of F. equiseti, the causal agent of fusarium wilt disease of C. asiatica in Bangladesh.展开更多
This work aims to screen mutant rice lines tolerant to Striga asiatica.Two rainfed sensitive rice varieties B22 and F154 were used.Plants survival rates of the two parents were significantly lower respectively(9.74a a...This work aims to screen mutant rice lines tolerant to Striga asiatica.Two rainfed sensitive rice varieties B22 and F154 were used.Plants survival rates of the two parents were significantly lower respectively(9.74a and 11.83a)than those of mutant lines(55.36c to 74.36b);Striga plants emergence/pot were significantly higher for the parents(13.96c and14.89c)compared to the mutants(0.12a to 1.5b);the infection rate of parents(7.37b;7.86b)was higher compared to the mutants(2.27a to 2.74a);fertility rate/plant of parents was lower(20.98%b;22.29%b)but much higher than mutants(72.19%b to 78.35%b);the average panicle number/plant of parents was significantly lower(0.5a;1a)than those of mutants(1.5b to 2.4bc)and the 100 g grain weight of parents are lower(2.35a;2.56a)than those of mutants(3.19b to 3.23b).The culture of those mutant lines may increase rice production and contribute to enhancing food security in Madagascar.展开更多
[Objectives]To study the anti-fatigue effect of water extract from Centella asiatica(L.)Urban on mice.[Methods]After intragastric administration of low,medium and high concentration of water extract from Centella asia...[Objectives]To study the anti-fatigue effect of water extract from Centella asiatica(L.)Urban on mice.[Methods]After intragastric administration of low,medium and high concentration of water extract from Centella asiatica(L.)Urban for 14 d,the rotarod time and the content of serum lactic acid in mice were determined,respectively.[Results]Compared with the control group,the rotarod time of mice in the low and medium concentration groups was significantly prolonged(P<0.05),but there was no significant difference between the control group and the high concentration group;the content of serum lactic acid in the medium concentration group was significantly lower than that in the control group(P<0.01),but there was no significant difference between low concentration group and high concentration group and the control group.[Conclusions]A certain concentration of water extract from Centella asiatica(L.)Urban had a good anti-fatigue effect.展开更多
A new triterpene and a saponin, named 2α,3β23-trihydroxyurs-20-en-28-oic acid （1） and 2α,3β,23-trihydroxyurs-20-en-28-oic acid O-α-L-rhanmopyranosyl-（1→4）-O-β-D-glucopyranosyl-（1→6）-O-β-D-glucopyranosyl...A new triterpene and a saponin, named 2α,3β23-trihydroxyurs-20-en-28-oic acid （1） and 2α,3β,23-trihydroxyurs-20-en-28-oic acid O-α-L-rhanmopyranosyl-（1→4）-O-β-D-glucopyranosyl-（1→6）-O-β-D-glucopyranosyl ester （2）, have been isolated from the aerial part of CenteUa asiatica. Their structures were elucidated by spectral methods, including 2D-NMR spectra.展开更多
Background:Bedsores are damage or loss of skin integrity due to prolonged pressure which there are several ways to treat and prevent them.The aim of the study was to determine the effect of Plantago major L.and Rosa d...Background:Bedsores are damage or loss of skin integrity due to prolonged pressure which there are several ways to treat and prevent them.The aim of the study was to determine the effect of Plantago major L.and Rosa damascene herbal ointment for bedsore healing in hospitalized patients.Methods:This clinical trial study was accomplished with 100 patients divided into control(50 patients)and intervention(50 patients)groups.The control group had received routine treatment(dressing with Comfeel),but the intervention group only had received herbal ointment and then both groups were compared with each other through the pressure ulcer scale for healing(PUSH)tool.Results:The mean and standard deviation of recovery time in the Comfeel group was 27.40±12.467 and in the herbal ointment group was 21.57±12.695.In the pre-intervention evaluation,the mean and standard deviation of the PUSH score in the herbal ointment group was 10.44±0.323 while in the last evaluation in the same group it was 0.68±0.288 but in the Comfeel group,in the pre-intervention evaluation,the mean and standard deviation of the PUSH score was 10.68±0.317,while in the last evaluation it was 1.98±0.472.The highest frequency of bedsores in the study units was in the sacral region(49%)and the lowest frequency was in the sole of the foot with 1%.Conclusion:The results showed that Plantago major L.and Rosa damascene herbal ointment was effective in accelerating recovery time and wound healing.展开更多
基金Supported by the National Natural Science Foundation of China(No.30873364)the Natural Science Foundation of Jilin Province of China(Nos.20071102 and 2008-167)
文摘Two phenylethanoid glycosides（PhGs）, plantamajoside and acteoside, were isolated and purified from the aerial parts ofPlantago asiatica for the first time by high performance centrifugal partition chromatography（HPCPC） with ethyl acetate, n-butanol, ethanol and water（0.5：0.5：0.1：1, volume ratio） as solvent system. A total of 45.6 mg of plantamajoside and 293.8 mg of acteoside were purified from 1341 mg of the n-butanol extract of P. asiatica, with a purity of 〉93.3% as determined by HPLC. The HPCPC fractions were analyzed by HPLC-DAD and the structures were identified by their retention time, UV, electrospray ionization multi stage tandem mass spectrometry（ESI-MSn） in the negative ion mode, and confirmed by NMR experiments. The characteristic fragment ions of ESI-MS of the two PhGs isolated from Plantago asiatica were discussed, which are specific and useful for the identification of the structures of PhGs.
基金the support of the National Natural Science Foundation of China(No.8187296 Authors 9)the Science and Youth Innovation Promotion Association of the CAS(Grant No.2019227)。
文摘Objective:This study aimed to elucidate the active components and mechanisms of Plantago asiatica L.(PAL)in the treatment of gout.Materials and Methods:Based on its polarity,PAL was partitioned into three parts,P1,P2,and P3,through gradient elution using AB-8 macroporous resin columns.The primary constituents of these fractions include iridoids,phenylethanoid glucosides,and flavonoids.Subsequently,a comprehensive in vitro and in vivo screening and evaluation model was developed considering the fundamental pathological processes of gout,including the inhibition of uric acid(UA)production,augmentation of UA excretion,and anti-inflammatory effects.This model was used to analyze the anti-gout activity of P1,P2,P3,and the overall extracts derived from PAL.Results:All the PAL parts exhibited specific therapeutic properties as part of gout therapy.Specifically,the P2 part demonstrated the ability to diminish interleukin-1 beta levels,lower UA concentrations,suppress joint inflammation in rats,and restore abnormal parameters associated with gout,such as creatinine and urea nitrogen.These findings highlight the significant effect of P2 on gout treatment.Conclusions:Following a thorough assessment of the outcomes derived from diverse anti-gout experiments,P2 exhibited superior anti-gout properties compared to the remaining parts.P2 demonstrated a restorative effect on the aberrant parameters associated with gout,thereby comprehensively elucidating the therapeutic mechanism of P2 against gout.This study provides substantial evidence and serves as a reference for future investigations regarding the material foundation of PAL in gout treatment.
基金supported by funding from Jilin Province (No.20060904-1 and 20071102).
文摘Two phenylethanoid glycosides （PhGs） were isolated and purified from the aerial parts of Plantago asiatica for the first time by high performance centrifugal partition chromatography （HPCPC） using ethyl acetate-n-butanol-ethanol-water （0.5：0.5：0.1：1, v/v/v/v）. A total of 45.6 mg of compound 1 and 293.8 mg of compound 2 were purified from 1341 mg of the n-butanol extract of P. asiatica. The structures of the two PhGs were tentatively identified as plantamajoside and aeteoside or isoacteoside by eleetrospray ionization multi stage tandem mass spectrometry （ESI-MS^n） in the negative ion mode.
文摘Box dimension and information dimension based on individual and leaf number were applied to analyze the distribution patterns of Plantato asiatica and P. viriginica in suburbs of Jinhua. The results showed that both the box dimension and the information dimention of P. virginica were obviously higher than those of P. asiatica in the high human-distributed environment. P. asiatica was mostly distributed in the environment with compacted soil. The information dimension on the basis of leaf probability in the boxes showed that the populations of P. virginica and P. asiatica in low density could exploit environmental resources effectively.
基金supported financially by the grants from the Chinese Academy of Sciences(KSCX2-EW-Q-10 and KSCX1-YW-R-24)the NSFC(No.20802082 and 30830115)+1 种基金the Major State Basic Research Development Program of China(No.2009CB522303 and 2009CB940900)the project of recruited top talent of sciences and technology of Yunnan Province(2006PY01-47).
文摘Four new indole alkaloids,plasiaticines A-D(1-4),together with two known ones,were isolated from the seeds of Plantago asiatica.The structures of the new compounds were elucidated on the basis of comprehensive analysis of spectroscopic data.All compounds were tested for their cytotoxic activity,and all compounds except 4 were tested for their acetylcholinesterase(AChE)inhibitory activities.
文摘The present study aimed at evaluation of prophylactic efficacy and possible mechanisms of asiaticoside （AS） based standardized extract of Centella asiatica （L.） Urban leaves （INDCA） in animal models of migraine. The effects of oral and intranasal （i.n.） pretreatment of INDCA （acute and 7-days subacute） were evaluated against nitroglycerine （NTG, 10 mg·kg^-1, i.p.） and bradykinin （BK, 10 μg, intra-arterial） induced hyperalgesia in rats. Tail flick latencies （from 0 to 240 rain） post-NTG treatment and the number of vocalizations post-BK treatment were recorded as a measure of hyperalgesia. Separate groups of rats for negative （Normal） and positive （sumatriptan, 42 mg.kg ^-1, s.c.） controls were included. The interaction of 1NDCA with selective 5-HT1A, 5-HT1B, and 5-HTI D receptor antagonists （NAN-190, Isamoltane hemifumarate, and BRL-15572 respectively） against NTG-induced hyperalgesia was also evaluated. Acute and sub-acute pre-treatment of INDCA [10 and 30 mg.kg^-1 （oral） and 100 μg/rat （i.n.） showed significant anti-nociception activity, and reversal of the NTG-induced hypera｜gesia and brain 5-HT concentration decline. Oral pre-treatment with INDCA （30 mg·kg ^-1, 7 d） showed significant reduction in the number of vocalization. The anti-nociceptive effects of INDCA were blocked by 5-HTIA and 5-HT1B but not 5-HT1D receptor antagonists. In conclusion, 1NDCA demonstrated promising anti-nociceptive effects in animal models of migraine, probably through 5-HT1A/1B medicated action.
基金support from National Natural Science Fund for Excellent Young Scholars(32222065)National Key Research and Development Program of China(2023YFF1103600)+3 种基金Key Research and Development Projects of Jiangxi Province(20244AFH82001)Key Research and Development Project of Jiangxi Province(20223BBF61023)National Key Research and Development Program of China(2023YFF1105400)The Central Guidance Fund for Local Science and Technology Development in Jiangxi Province(20252ZDD020002)were gratefully acknowledged.
文摘Understanding polysaccharide-gut microbe interactions is critical for elucidating their health benefits for the host.This study aimed to investigate the interaction between polysaccharides from Plantago asiatica L.seeds(PLCP)and Bacteroides by using antibiotics induced pseudo-sterile mice that gavaged with Bacteroides strains for 5 days and then switched to 7 days of PLCP,based on the changes of gut microbiota,short-chain fatty acids(SCFAs)and serum biochemical parameters.Seven Strains from different species with good PLCP utilization ability successfully survived in the gut of these mice.The additional PLCP tended to significantly and differently impact the relative abundance of Bacteroides only in B.thetaiotaomicron C1 and B.salyersiae C13 treated mice(0.05<P<0.1).LEfSe analysis further suggested strain-specific interaction consequence with PLCP on microbiota.Notably,B.koreensis C16 and B.salyersiae C13 significantly elevated total colonic SCFAs levels,respectively,mainly due to the increase in acetic acid,which were not affected by further PLCP treatment.PLCP significantly decreased propionic-and increased butyric acid levels in mice recieving B.koreensis C16,while the former was also seen in B.fragilis C11 and the latter in B.salyersiae C13 treated ones.Moreover,only B.salyersiae C13+PLCP treatment significantly elevated serum total protein,albumin,and HDL-C levels in mice.These findings establish a theoretical framework for elucidating the mechanisms underlying PLCP-Bacteroides in-teractions,providing fundamental insights for future mechanistic studies and therapeutic development targeting gut microbiota.
文摘Antimicrobial agents such as bavistin, cefotaxime and kanamycin were evaluated for their effects on the rapid shoot regeneration from nodal explants of Centella asiatica (L.). Filter sterilized bavistin (250 mg/L) was augmented alone and in combination with cytokinins such as BAP and TDZ into the media to trace the effect on regeneration. On this basis, the potential use of bavistin (150 mg/L) along with BAP (2.0 mg/L) was evaluated which showed the maximum shoot number (6.6) and shoot length (4.4 cm) respectively. Cefotaxime at the concentration of 100 μM/L was found to be effective to obtain the maximum shoot number formation (5.8) with the regeneration frequency (90%). Kanamycin at the concentration of 80 μM/L induced maximum shoot regeneration (5.12). Kanamycin at 100 μM/L or at higher concentrations reduced the shoot regeneration. The best rooting response was noticed when in vitro regenerated microshoots were transferred to the rooting medim which was supplemented with IBA (2.0 mg/L). This combination generates 90% of regeneration frequency and maximum number of roots per shoot (14.2) and high root length (4.2 cm). The rooted plants were acclimatized and transferred to field for survivalance. The addition of antibiotics was found to be more effective and safer for using since their effects on regeneration were found to be negligible.
文摘In this study we designed a novel,cost‐efficient and green method for the synthesis of copper nanoparticles(Cu NPs)supported on manganese dioxide(MnO2)NPs,using Centella asiatica L.leaf extract as a naturally‐sourced reducing agent,without stabilizers or surfactants.This synthetic process is environmentally‐friendly and avoids the use of toxic reducing agents.Phenolic hydroxyl groups in the leaf extract are believed to reduce Cu2+in solution to generate Cu NPs that are subsequently stabilized on the MnO2NP surfaces.The resulting Cu/MnO2nanocomposite was fully characterized using X‐ray diffraction,transmission electron microscopy,field emission scanning electron microscopy,energy‐dispersive X‐ray spectroscopy and Fourier transform infrared spectroscopy.This material was found to function as a highly active,efficient and recyclable heterogeneous catalyst for the reduction of Congo red,rhodamine B and methylene blue as well as nitro compounds such as2,4‐dinitrophenylhydrazine and4‐nitrophenol in the presence of NaBH4in aqueous media at ambient temperature.The high stability of the Cu/MnO2nanocomposite also allows the catalyst to be separated and reused several times without any significant loss of activity.?2018,Dalian Institute of Chemical Physics,Chinese Academy of Sciences.Published by Elsevier B.V.All rights reserved.
文摘Objective:To investigate the antifertility properties ofCentella asiatica L. ethanolic extract and identify the sperm proteomic changes in treated male rats.Methods: A total of 32 male Sprague-Dawley rats were divided into two groups: control group (n=16) received distilled water and treatment group (n=16) which received 300 mg/kg ofCentella asiatica L. extract for 42 d. After the treatment period, the number of implantation sites was recorded and the sperm proteomic changes were analysed by 2D gel electrophoresis. In addition, the expression of protein spots was quantified by MALDI-TOF analysis.Results:Centella asiatica L. extract resulted in low number of implantation sites in the treatment group (100.00±2.82) compared to the control group (183.00±2.14). The percentage of infertile male rats in the treatment group was higher (43.75%) compared to the control group (18.75%). Proteomic analysis showed the expression of protein spots identified in the treatment group decreased with 234 spots compared to the control group with 282 spots.Conclusions:The results revealed that there was antifertility activity in the male rats with the administration ofCentella asiatica L. ethanol extract and the identified proteins could provide understanding on the adverse effect ofCentella asiaticain male reproductive system.
文摘Centella asiatica (L.) is commonly known as Thankuni plant and has ethnobotanical importance in Bangladesh. Present experiment was conceded to investigate the wilt disease of C. asiatica, vegetative growth and molecular characterization of pathogenic fungi. Pathogenic fungus, Fusarium equiseti was identified as a causal agent of wilt disease in C. asiatica. The effect of culture media on the mycelial growth of F. equiseti showed the highest (89.25 mm) on potato dextrose agar (PDA) medium followed by carrot agar (CA) medium and the lowest growth (40.25 mm) was measured in HA medium. The optimal temperature and pH for mycelial growth of F. equiseti were 30°C and 7, respectively. The genetic variation of the selected species of fungi, the internal transcribed spacer (ITS) region was amplified using ITS4 and ITS5 primers and sequenced. The PCR product of the ITS region of F. equiseti was 535 bp. Phylogenetic tree of thirty-seven strains of Fusarium sp. based on the nucleotide sequences of the ITS region using the neighbor-joining method with 1000 bootstrapping indicated that 98% - 100% identity with MN886590.1 JUF0046 (F. equiseti). ITS sequences are generally constant, or show little variation within species, but vary between species in a genus. The ITS region is relatively short and can be easily amplified by PCR using universal single primer pairs. Genetic distance exhibited high level of similarity with identical ITS sequences. To date, no published research articles are found on the molecular identification of F. equiseti, the causal agent of fusarium wilt disease of C. asiatica in Bangladesh.
文摘This work aims to screen mutant rice lines tolerant to Striga asiatica.Two rainfed sensitive rice varieties B22 and F154 were used.Plants survival rates of the two parents were significantly lower respectively(9.74a and 11.83a)than those of mutant lines(55.36c to 74.36b);Striga plants emergence/pot were significantly higher for the parents(13.96c and14.89c)compared to the mutants(0.12a to 1.5b);the infection rate of parents(7.37b;7.86b)was higher compared to the mutants(2.27a to 2.74a);fertility rate/plant of parents was lower(20.98%b;22.29%b)but much higher than mutants(72.19%b to 78.35%b);the average panicle number/plant of parents was significantly lower(0.5a;1a)than those of mutants(1.5b to 2.4bc)and the 100 g grain weight of parents are lower(2.35a;2.56a)than those of mutants(3.19b to 3.23b).The culture of those mutant lines may increase rice production and contribute to enhancing food security in Madagascar.
基金Supported by The Innovation and Entrepreneurship Training Program for College Students in Guangxi Autonomous Region(S202110599035)Guangxi Key R&D Program(GuiKe AB18221095)High-level Talent Research Project of Youjiang Medical University for Nationalities(01002018079).
文摘[Objectives]To study the anti-fatigue effect of water extract from Centella asiatica(L.)Urban on mice.[Methods]After intragastric administration of low,medium and high concentration of water extract from Centella asiatica(L.)Urban for 14 d,the rotarod time and the content of serum lactic acid in mice were determined,respectively.[Results]Compared with the control group,the rotarod time of mice in the low and medium concentration groups was significantly prolonged(P<0.05),but there was no significant difference between the control group and the high concentration group;the content of serum lactic acid in the medium concentration group was significantly lower than that in the control group(P<0.01),but there was no significant difference between low concentration group and high concentration group and the control group.[Conclusions]A certain concentration of water extract from Centella asiatica(L.)Urban had a good anti-fatigue effect.
文摘A new triterpene and a saponin, named 2α,3β23-trihydroxyurs-20-en-28-oic acid （1） and 2α,3β,23-trihydroxyurs-20-en-28-oic acid O-α-L-rhanmopyranosyl-（1→4）-O-β-D-glucopyranosyl-（1→6）-O-β-D-glucopyranosyl ester （2）, have been isolated from the aerial part of CenteUa asiatica. Their structures were elucidated by spectral methods, including 2D-NMR spectra.
基金All the ethical considerations based on the International Ethical Protocols were considered by the authors and the work was approved by the ethics committee of the Khomein of Medical Sciences University(Approval code:IR.KHOMEIN.REC.1397.003)This study has also been registered in the Iranian Registry of Clinical Trials(IRCT20100130003227N13)on May 24,2019.
文摘Background:Bedsores are damage or loss of skin integrity due to prolonged pressure which there are several ways to treat and prevent them.The aim of the study was to determine the effect of Plantago major L.and Rosa damascene herbal ointment for bedsore healing in hospitalized patients.Methods:This clinical trial study was accomplished with 100 patients divided into control(50 patients)and intervention(50 patients)groups.The control group had received routine treatment(dressing with Comfeel),but the intervention group only had received herbal ointment and then both groups were compared with each other through the pressure ulcer scale for healing(PUSH)tool.Results:The mean and standard deviation of recovery time in the Comfeel group was 27.40±12.467 and in the herbal ointment group was 21.57±12.695.In the pre-intervention evaluation,the mean and standard deviation of the PUSH score in the herbal ointment group was 10.44±0.323 while in the last evaluation in the same group it was 0.68±0.288 but in the Comfeel group,in the pre-intervention evaluation,the mean and standard deviation of the PUSH score was 10.68±0.317,while in the last evaluation it was 1.98±0.472.The highest frequency of bedsores in the study units was in the sacral region(49%)and the lowest frequency was in the sole of the foot with 1%.Conclusion:The results showed that Plantago major L.and Rosa damascene herbal ointment was effective in accelerating recovery time and wound healing.
