Leaf spot disease caused by Alternaria tenuissima seriously affects the yields of medicinal roots and cut flowers of Paeonia lactiflora.To mine the leaf spot resistance genes in P.lactiflora,we employed rapid amplific...Leaf spot disease caused by Alternaria tenuissima seriously affects the yields of medicinal roots and cut flowers of Paeonia lactiflora.To mine the leaf spot resistance genes in P.lactiflora,we employed rapid amplification of cDNA ends(RACE)to clone PlPR1 from the leaves of P.lactiflora.The results of bioinformatics analysis showed that the coding region of PlPR1 was 522 bp in length,and the encoded protein contained a typical CAP domain.Overexpression of PlPR1 in tobacco significantly decreased the disease index,delayed the disease progression,and attenuated the hypersensitive response induced by pathogen invasion in the transgenic plants.In conclusion,PlPR1 enhanced the plant resistance to the pathogen A.tenuissima by regulating the immune responses of plants.The findings provided a candidate target gene and a theoretical basis for breeding P.lactiflora varieties with disease resistance.展开更多
文摘Leaf spot disease caused by Alternaria tenuissima seriously affects the yields of medicinal roots and cut flowers of Paeonia lactiflora.To mine the leaf spot resistance genes in P.lactiflora,we employed rapid amplification of cDNA ends(RACE)to clone PlPR1 from the leaves of P.lactiflora.The results of bioinformatics analysis showed that the coding region of PlPR1 was 522 bp in length,and the encoded protein contained a typical CAP domain.Overexpression of PlPR1 in tobacco significantly decreased the disease index,delayed the disease progression,and attenuated the hypersensitive response induced by pathogen invasion in the transgenic plants.In conclusion,PlPR1 enhanced the plant resistance to the pathogen A.tenuissima by regulating the immune responses of plants.The findings provided a candidate target gene and a theoretical basis for breeding P.lactiflora varieties with disease resistance.
