摘要
Leaf spot disease caused by Alternaria tenuissima seriously affects the yields of medicinal roots and cut flowers of Paeonia lactiflora.To mine the leaf spot resistance genes in P.lactiflora,we employed rapid amplification of cDNA ends(RACE)to clone PlPR1 from the leaves of P.lactiflora.The results of bioinformatics analysis showed that the coding region of PlPR1 was 522 bp in length,and the encoded protein contained a typical CAP domain.Overexpression of PlPR1 in tobacco significantly decreased the disease index,delayed the disease progression,and attenuated the hypersensitive response induced by pathogen invasion in the transgenic plants.In conclusion,PlPR1 enhanced the plant resistance to the pathogen A.tenuissima by regulating the immune responses of plants.The findings provided a candidate target gene and a theoretical basis for breeding P.lactiflora varieties with disease resistance.
由细极链格孢(Alternaria tenuissima)引起的芍药叶斑病严重影响芍药药用根部与切花产量。为挖掘芍药抗叶斑病相关基因,本研究通过cDNA末端快速克隆技术(RACE)从芍药叶片中克隆获得PlPR1基因。生物信息学分析显示,其编码区(CDS)全长522 bp,编码的蛋白含有典型CAP超家族结构域。通过构建PlPR1过表达载体并转化烟草,接种病原菌后的表型分析表明:转基因植株的病情指数显著降低,病害扩展速率延缓,且病原菌侵染诱发的超敏反应(HR)明显减弱。上述结果证实,PlPR1通过调控植物免疫反应增强了对细极链格孢菌的抗性。本研究为芍药抗病育种提供了候选基因靶点和理论依据。
基金
山东省自然科学基金项目(ZR2021MC007)。
