From the fungus Trichoderma sp.,we isolated seven novel 18-residue peptaibols,neoatroviridins E-K(1−7),and six new 14-residue peptaibols,harzianins NPDG J-O(8−13).Additionally,four previously characterized 18-residue ...From the fungus Trichoderma sp.,we isolated seven novel 18-residue peptaibols,neoatroviridins E-K(1−7),and six new 14-residue peptaibols,harzianins NPDG J-O(8−13).Additionally,four previously characterized 18-residue peptaibols neoatroviridins A-D(14−17)were also identified.The structural configurations of the newly identified peptaibols(1−13)were determined by comprehensive nuclear magnetic resonance(NMR)and high-resolution electrospray ionization tandem mass spectrometry(HR-ESI-MS/MS)data.Their absolute configurations were further determined using Marfey’s method.Notably,compounds 12 and 13 represent the first 14-residue peptaibols containing an acidic amino acid residue.In antimicrobial assessments,all 18-residue peptaibols(1−7,14−17)exhibited moderate inhibitory activities against Staphylococcus aureus 209P,with minimum inhibitory concentration(MIC)values ranging from 8−32μg·mL^(−1).Moreover,compound 9 exhibited moderate inhibitory effect on Candida albicans FIM709,with a MIC value of 16μg·mL^(−1).展开更多
The use of microorganisms for biological purposes has become an effective alternative to control plant pathogens. Trichoderma koningii Smf2 was chosen from eight Trichoderma strains for its thermostatic metabolites wi...The use of microorganisms for biological purposes has become an effective alternative to control plant pathogens. Trichoderma koningii Smf2 was chosen from eight Trichoderma strains for its thermostatic metabolites with antibiotic activity against Ralstonia solancearum Smith. Exclusion chromatography (LH20) was used twice to partially purify targeted metabolites combined with biological test. LC/ESI-MS, a powerful tool for rapid identification and sequence determination of peptides, identified these metabolites as three peptaibols named Trichokonin Ⅵ,Ⅶ and Ⅷ, and their sequences were confirmed with NMR.展开更多
Antibiotic resistance is spreading at a faster rate than new antibiotic agents applied for clinical remedies.It is an urgent need to discover potential compounds to combat multidrug-resistant(MDR)bacteria.Marine fungi...Antibiotic resistance is spreading at a faster rate than new antibiotic agents applied for clinical remedies.It is an urgent need to discover potential compounds to combat multidrug-resistant(MDR)bacteria.Marine fungi offer a promising avenue for mining antibiotic-like molecules with chemical diversity.To discover structurally novel and antibiotic metabolites,we screened the in-house marine fungus genome library and found a fungus Stephanonectria keithii LZD-10-1 containing a non-ribosomal peptide synthetase(NRPS)cluster with 18 modules to synthesize a new subfamily of peptaibols with effective eradication against MDR pathogens.Targeting isolation of the cultured fungus afforded six new peptaibols,which exhibit the ability to kill MDR bacteria by targeting bacterial membrane phospholipids,especially phosphatidylglycerol(PG),leading to the dysfunction of bacterial membranes.Furthermore,their efficacies against methicillin-resistant Staphylococcus aureus(MRSA)in both Galleria mellonella and mouse wound infection models were observed.This study underscores the significance of employing genome-guided approaches to identify untapped marine fungi as potential sources for novel antibiotic candidates with unique scaffolds.展开更多
Peptaibols are a large family of linear,amphipathic polypeptides consisting of 5-20 amino acid residues gener-ated from the fungal nonribosomal peptide synthetase(NRPS)pathway.With a relatively high content of non-pro...Peptaibols are a large family of linear,amphipathic polypeptides consisting of 5-20 amino acid residues gener-ated from the fungal nonribosomal peptide synthetase(NRPS)pathway.With a relatively high content of non-proteinogenic amino acids such as𝛼-aminoisobutyrate(Aib)and isovaline(Iva)in the skeleton,peptaibols exhibit a wide range of biological activities,including anti-microbial,cytotoxic,and neuroleptic effects.With five peptai-bols brought to market for use as biocontrol agents,this class of peptides has received increasing attention from both biochemists and pharmacologists.In this review,we summarized the progress made in structural character-ization,elucidation of biosynthetic pathways,and investigation of biosynthesis elucidation and bioactivities,to promote further efforts to develop peptaibols as pharmaceuticals.展开更多
Hypocrea/Trichoderma is a genus of soil-borne or wood-decaying fungi containing members important to mankind as producers of industrial enzymes and biocontrol agents against plant pathogens, but also as opportunistic ...Hypocrea/Trichoderma is a genus of soil-borne or wood-decaying fungi containing members important to mankind as producers of industrial enzymes and biocontrol agents against plant pathogens, but also as opportunistic pathogens of immuno- compromised humans and animals, while others can cause damage to cultivated mushroom. With the recent advent of a reliable, BarCode-aided identification system for all known taxa of Trichoderma and Hypocrea, it became now possible to study some of the biological fundamentals of the diversity in this fungal genus in more detail. In this article, we will therefore review recent progress in (1) the understanding of the geographic distribution of individual taxa; (2) mechanisms of speciation leading to development of mushroom diseases and facultative human mycoses; and (3) the possible correlation of specific traits of secondary metabolism and molecular phylogeny.展开更多
Plant viruses are the most infectious agents in commercially important crops worldwide. Plant viral diseases are important because both decreased yielding and quality of fruits, flowers or vegetables lead to million-d...Plant viruses are the most infectious agents in commercially important crops worldwide. Plant viral diseases are important because both decreased yielding and quality of fruits, flowers or vegetables lead to million-dollar losses in production. At present there are no reports which suggest a direct control of plant virus. A new strategy for plant virus control has been raised since 13 years ago—the use of peptides. Peptides could offer a direct interaction by affinity selection against viral proteins involved in infection cycle, like capsid or movement protein (e.g.) and affect viral replication. Peptidomics, as a new tool to study peptides, led us screening and selecting the best peptide with antiviral activity, and re-designing it to enhance the biological effect as well as the potential of bioactivity of those peptides secreted by microbes present in soil. In this paper we review current aspects in the use of peptides and peptidomics as a strategy to study new methods that lead a direct control against plant viral diseases.展开更多
基金supported by the National Key Research and Development Program of China(No.2018YFA0903200//2018YFA0903201)the National Natural Science Foundation of China(Nos.81925037 and 81973213)+6 种基金National High-level Personnel of Special Support Program(No.2017RA2259)the 111 Project of Ministry of Education of the People's Republic of China(No.B13038)Local Innovative and Research Teams Project of Guangdong Pearl River Talents Program(No.2017BT01Y036)Guangdong International Science and Technology Cooperation Base(No.2021A0505020015)Innovative and Research Teams Project of Guangdong Higher Education Institution(No.2021KCXTD001)K.C.Wong Education Foundation(No.Guo-Dong Chen,2021,China)the Science and Technology Planning Project of Guangzhou(No.202201020048)。
文摘From the fungus Trichoderma sp.,we isolated seven novel 18-residue peptaibols,neoatroviridins E-K(1−7),and six new 14-residue peptaibols,harzianins NPDG J-O(8−13).Additionally,four previously characterized 18-residue peptaibols neoatroviridins A-D(14−17)were also identified.The structural configurations of the newly identified peptaibols(1−13)were determined by comprehensive nuclear magnetic resonance(NMR)and high-resolution electrospray ionization tandem mass spectrometry(HR-ESI-MS/MS)data.Their absolute configurations were further determined using Marfey’s method.Notably,compounds 12 and 13 represent the first 14-residue peptaibols containing an acidic amino acid residue.In antimicrobial assessments,all 18-residue peptaibols(1−7,14−17)exhibited moderate inhibitory activities against Staphylococcus aureus 209P,with minimum inhibitory concentration(MIC)values ranging from 8−32μg·mL^(−1).Moreover,compound 9 exhibited moderate inhibitory effect on Candida albicans FIM709,with a MIC value of 16μg·mL^(−1).
文摘The use of microorganisms for biological purposes has become an effective alternative to control plant pathogens. Trichoderma koningii Smf2 was chosen from eight Trichoderma strains for its thermostatic metabolites with antibiotic activity against Ralstonia solancearum Smith. Exclusion chromatography (LH20) was used twice to partially purify targeted metabolites combined with biological test. LC/ESI-MS, a powerful tool for rapid identification and sequence determination of peptides, identified these metabolites as three peptaibols named Trichokonin Ⅵ,Ⅶ and Ⅷ, and their sequences were confirmed with NMR.
基金supported by National Key Research and Development Program of China(2022YFD1801600,2022YFC2804900)National Natural Science Foundation of China(81991525,32230106)China Postdoctoral Science Foundation(2022M710266).
文摘Antibiotic resistance is spreading at a faster rate than new antibiotic agents applied for clinical remedies.It is an urgent need to discover potential compounds to combat multidrug-resistant(MDR)bacteria.Marine fungi offer a promising avenue for mining antibiotic-like molecules with chemical diversity.To discover structurally novel and antibiotic metabolites,we screened the in-house marine fungus genome library and found a fungus Stephanonectria keithii LZD-10-1 containing a non-ribosomal peptide synthetase(NRPS)cluster with 18 modules to synthesize a new subfamily of peptaibols with effective eradication against MDR pathogens.Targeting isolation of the cultured fungus afforded six new peptaibols,which exhibit the ability to kill MDR bacteria by targeting bacterial membrane phospholipids,especially phosphatidylglycerol(PG),leading to the dysfunction of bacterial membranes.Furthermore,their efficacies against methicillin-resistant Staphylococcus aureus(MRSA)in both Galleria mellonella and mouse wound infection models were observed.This study underscores the significance of employing genome-guided approaches to identify untapped marine fungi as potential sources for novel antibiotic candidates with unique scaffolds.
基金This work was funded by National Natural Science Foundation of China(Nos.41976105,81991522)Taishan Scholar Youth Expert Pro-gram in Shandong Province(No.tsqn 202103153)+4 种基金Major Basic Re-search Programs of Natural Science Foundation of Shandong Province(No.ZR2019ZD18)the Fundamental Research Funds for the Cen-tral Universities(No.201941001)the NSFC-Shandong Joint Fund(No.U1906212)the Marine S&T Fund of Shandong Province for Pi-lot National Laboratory for Marine Science and Technology(Qingdao)(No.2018SDKJ0401-2)the Youth Innovation Plan of Shandong province(No.2019KJM004).
文摘Peptaibols are a large family of linear,amphipathic polypeptides consisting of 5-20 amino acid residues gener-ated from the fungal nonribosomal peptide synthetase(NRPS)pathway.With a relatively high content of non-proteinogenic amino acids such as𝛼-aminoisobutyrate(Aib)and isovaline(Iva)in the skeleton,peptaibols exhibit a wide range of biological activities,including anti-microbial,cytotoxic,and neuroleptic effects.With five peptai-bols brought to market for use as biocontrol agents,this class of peptides has received increasing attention from both biochemists and pharmacologists.In this review,we summarized the progress made in structural character-ization,elucidation of biosynthetic pathways,and investigation of biosynthesis elucidation and bioactivities,to promote further efforts to develop peptaibols as pharmaceuticals.
基金Project (Nos.FWF P-12748-MOB,FWF P-16601-CPK,and FWFP-17895-ISD) supported by the Austrian Science Fund Grants
文摘Hypocrea/Trichoderma is a genus of soil-borne or wood-decaying fungi containing members important to mankind as producers of industrial enzymes and biocontrol agents against plant pathogens, but also as opportunistic pathogens of immuno- compromised humans and animals, while others can cause damage to cultivated mushroom. With the recent advent of a reliable, BarCode-aided identification system for all known taxa of Trichoderma and Hypocrea, it became now possible to study some of the biological fundamentals of the diversity in this fungal genus in more detail. In this article, we will therefore review recent progress in (1) the understanding of the geographic distribution of individual taxa; (2) mechanisms of speciation leading to development of mushroom diseases and facultative human mycoses; and (3) the possible correlation of specific traits of secondary metabolism and molecular phylogeny.
文摘Plant viruses are the most infectious agents in commercially important crops worldwide. Plant viral diseases are important because both decreased yielding and quality of fruits, flowers or vegetables lead to million-dollar losses in production. At present there are no reports which suggest a direct control of plant virus. A new strategy for plant virus control has been raised since 13 years ago—the use of peptides. Peptides could offer a direct interaction by affinity selection against viral proteins involved in infection cycle, like capsid or movement protein (e.g.) and affect viral replication. Peptidomics, as a new tool to study peptides, led us screening and selecting the best peptide with antiviral activity, and re-designing it to enhance the biological effect as well as the potential of bioactivity of those peptides secreted by microbes present in soil. In this paper we review current aspects in the use of peptides and peptidomics as a strategy to study new methods that lead a direct control against plant viral diseases.
