Objective:To investigate the effects of pectic polysaccharides extracted from Rauwolfia verticillata(Lour.) Baill.var.hainanensis Tsiang on an experimental murine colitis model.Methods:Experimental colitis was induced...Objective:To investigate the effects of pectic polysaccharides extracted from Rauwolfia verticillata(Lour.) Baill.var.hainanensis Tsiang on an experimental murine colitis model.Methods:Experimental colitis was induced by dextran sulfate sodium(DSS),and mice were divided into 4 groups:control.DSS alone.DSS plus SASP,DSS plus pectic polysaccharides.The disease activity index(DAI) and histological score were observed.The tumor necrosis factor(TNF)-α and interleukin(IL)-17 levels were measured by enzyme-linked immunosorbent assay.I κ B and NF-κB p65 expression were assessed by western blot analysis.Myeloperoxidase(MPO) activity was determined by using MPO assay kit.Re.sults:Administration of pectic polysaccharides significantly reduced the severity of DSS-induced colitis as assessed by DAT and histological score,and resulted in down regulation of MPO activity and NF-κB p65 expression and subsequent degradation of IκB protein,strikingly reduced the production of TNF-α and IL-17.Conclusions:Pectic polysaccharides extracted from Rauvolfia verticillata(Lour.)Baill.var.hainanensis Tsiang exerts beneficial effects in experimental colitis and may therefore provide a useful therapeutic approach for the treatment of UC.展开更多
AIM: To investigate H+, K+-ATPase inhibition, anti-H pylori , antioxidant, and the in vivo antiulcer potential of a pectic polysaccharide from Swallow root (Decalepis hamiltonii; SRPP). METHODS: SRPP, with known sugar...AIM: To investigate H+, K+-ATPase inhibition, anti-H pylori , antioxidant, and the in vivo antiulcer potential of a pectic polysaccharide from Swallow root (Decalepis hamiltonii; SRPP). METHODS: SRPP, with known sugar composition [rhamnose: arabinose: xylose: galactose in the ratio of 16:50:2:32 (w/w), with 141 mg/g of uronic acid] was examined for anti-ulcer potency in vivo against swim/ ethanol stress-induction in animal models. Ulcer index, antioxidant/antioxidant enzymes, H+, K+-ATPase and gastric mucin levels were determined to assess the anti- ulcer potency. Anti-H pylori activity was also determined by viable colony count and electron microscopic studies. RESULTS: SRPP, containing phenolics at 0.12 g GAE/g, prevented stress-induced gastric ulcers in animal models by 80%-85%. Down regulation of gastric mucin 2-3 fold, antioxidant/antioxidant enzymes and upregulation of 3 fold of H+, K+-ATPase in ulcerous animals were normalized upon treatment with SRPP. Histopathological analysis revealed protection to the disrupted gastric mucosal layer and epithelial glands. SRPP also inhibited H+, K+-ATPase in vitro, at an IC50 of 77 μg/mL as opposed to that of 19.3 μg/mL of Lansoprazole and H pylori growth at Minimum Inhibitory Concentration (MIC) of 150 μg/mL. In addition, free radical scavenging (IC50-40 μg/mL) and reducing power (3200 U/g) activities were also observed. CONCLUSION: SRPP, with defined sugar composition and phenolics, exhibited multi-potent free radical scavenging, antioxidant, anti-H pylori, inhibition of H+, K+-ATPase and gastric mucosal protective activities. In addition, SRPP is non-toxic as opposed to other known anti-ulcer drugs, and therefore may be employed as a potential alternative for ulcer management.展开更多
Background and objectives:High-pressure processing(HPP)is a promising assistive method to extract pectic polysaccharides with high rhamnogalacturonan I(RG-I)domain and berries are sources of such pectic polysaccharide...Background and objectives:High-pressure processing(HPP)is a promising assistive method to extract pectic polysaccharides with high rhamnogalacturonan I(RG-I)domain and berries are sources of such pectic polysaccharides.This study extracts pectic polysaccharides from goji berry,raspberry,and cranberry,examines how HPP influences the pectic polysaccharide structure of three berries,and provides a basis for the extraction and modification of pectic polysaccharides with specific structure and bioactivity.Materials and methods:An orthogonal test was performed to optimize the HPP-assisted alkali method to extract the high yield and high RG-I content pectic polysaccharides from three berries.Structural information of pectic polysaccharides extracted by the HPP method and conventional methods were compared from the perspectives of monosaccharide composition,molecular weight,Fourier transform infrared spectroscopy,andnuclearmagneticresonancespectroscopy.Results:For raspberry,the optimal conditions consisted of a pressure of 50o MPa,a pH of 13,and a pressure-holding time of 12 min,while the optimal conditions for goji berry and cranberry were both 40o MPa,pH 13,and 15 min.Under the optimal conditions,the yields for goji berry,raspberry,and cranberry were 10.49%,16.63%,and 17.52%,respectively,and RG-l contents were 81.85%,83.30%,and 63.22%,respectively.HPP showed an effect to degrade homogalacturonan backbones and side chains and increase the RG-I content to some extent.Conclusion:HPP-assisted alkali method was revealed to be an efficient method to extract high RG-I content pectic polysaccharides,especially for cranberry,and was a potential method to modify pectic polysaccharide structure in a certain way.展开更多
As a kind of green additive,pectic oligosaccharide(POS) may regulate some physiological functions of animals,such as gut health,antioxidant capacity,immunity and lipid metabolism.This study aimed to identify whether P...As a kind of green additive,pectic oligosaccharide(POS) may regulate some physiological functions of animals,such as gut health,antioxidant capacity,immunity and lipid metabolism.This study aimed to identify whether POS administration can improve maternal reproduction,and to determine the possible metabolism.A total of 48 pregnant Wistar rats randomly allotted into 2 groups,and each group was fed a diet supplemented with 0 or 800 mg/kg of POS.Pectic oligosaccharide administration increased rat born number(P <0.05),did not affect rat embryo number on d 7 of gestation,but increased rat fetus number on d 14 of gestation(P <0.05).On d 14 of gestation,POS treatment improved Lactobacillus and Bifidobacterium populations and volatile fatty acid concentrations of cecal digesta(P <0.05),hormone(progesterone and nitric oxide) and cytokine(interleukin 2) concentrations of serum(P <0.05),and antioxidant capacity of serum(increased total antioxidant capacity and decreased malondialdehyde) and placenta(increased total superoxide dismutase,decreased malondialdehyde)(P <0.05) in pregnant rats.These results suggest that POS administration improved rat reproduction via decreasing fetus loss in middle gestation.This was due to the increased volatile fatty acid concentrations in rat gut improving hormone and inflammatory-cytokine productions,and antioxidant capacity.展开更多
The aim of this study was to continuously hydrolyze mango peel pectin,an agricultural waste,into pectic oligosaccharides(POS)with antibacterial activity.A packed-bed reactor(PBR)with immobilized polygalacturonase was ...The aim of this study was to continuously hydrolyze mango peel pectin,an agricultural waste,into pectic oligosaccharides(POS)with antibacterial activity.A packed-bed reactor(PBR)with immobilized polygalacturonase was designed,and the effects of the process conditions(flow rate,substrate concentration,and enzyme amount)were evaluated.The optimal conditions were obtained using response surface analysis,and a stable POS production with a yield of 94.56%was achieved.The PBR retained 82%of its initial activity after continuous operation for 72 h.The POS products exhibited good antibacterial activity against E.coli,S.aureus,B.subtilis,and S.typhimurium.The destructive effects of POS on the membrane system integrity were observed via scanning electron microscopy and the leakage of intracellular nucleic acids.Thus,the PBR is a powerful tool for continuous POS production,and hence,it can be applied as an alternative to chemical preservatives in the food industry.展开更多
In the last years, sugar beet pectins have been the subject of several investigations involving extraction methodologies, chemical composition and functional properties. The structure of pectins, which depends on the ...In the last years, sugar beet pectins have been the subject of several investigations involving extraction methodologies, chemical composition and functional properties. The structure of pectins, which depends on the extraction method, is decisive in their capacity to induce apoptosis on several cancer cell lines like colon, prostate and breast. In this work, sugar beet pectin extraction was performed in the following steps： lipid extraction with hexane, removal of soluble complex carbohydrates and proteins, and enzymatic treatment with amyloglucosidase, protease, and pectinase. The enzymatic treatment was carried out with Rohapect DA6L under the following conditions： 50℃, pH 4.0, 2% enzyme/substrate （E/S） ratio, 15 h, and a solid to liquid ratio of 1 ： 10. The pectic extract showed a degree of polymerization （DP） profile of 55.8% with DP≥7; 4.9% with DP6; 5.8% between DP2 and DP6 ; 4.7% with DP2; and 28.8% with DP1. The pectic extract was examined for its antiproliferative activity on the MCF-7 breast cancer cell line. At a concentration range of 12.5-25 mg/mL the pectic extract killed 80.6% of the cells, exhibiting a higher antiproliferative activity than 4-hydroxytamoxifen （4- OHT）, a classical anticancer drug, which killed 56.5% of the cells.展开更多
The present study was carried out to evaluate the effects of different concentrations of pectic oligosaccharide(POS)on the physicochemical properties of Lactobacillus bulgaricus(L.bulgaricus)loaded-synbiotic hydrogel ...The present study was carried out to evaluate the effects of different concentrations of pectic oligosaccharide(POS)on the physicochemical properties of Lactobacillus bulgaricus(L.bulgaricus)loaded-synbiotic hydrogel beads and the survival of L.bulgaricus.Low methoxyl(LM)pectin-resistant starch(RS)hydrogel beads,named RS beads,were coated/filled with different concentrations of POS:0.8%(RS-POS0.8 beads),1.0%(RS-POS1.0 beads),and 1.2%(RS-POS1.2 beads).POS treatment resulted in smooth beads with less surface dent by hydrogen bonding between RS beads and POS,which was confirmed by SEM and XRD analyses.The encapsulation efficiency of RS beads and RS-POS beads ranged from 90.40%to 92.03%.In simulated gastric fluid(SGF)and bile salt solution,free L.bulgaricus completely died but L.bulgaricus encapsulated in RS-POS1.2 beads only slightly decreased.This indicates that RS-POS1.2 beads exhibited higher protective effects on L.bulgaricus under SGF condition and bile salts.Furthermore,considering the relatively higher storage stability of L.bulgaricus loaded in RS-POS1.2 beads,the present results suggest that RS beads coated/filled with POS could be used for synbiotic encapsulation,protection,and delivery of probiotics.展开更多
In this study,the improvement effects of oral administration of polysaccharides isolated from Saururus chinensis on a DSS-induced ulcerative colitis mouse model were evaluated.To achieve this,S.chinensis-derived crude...In this study,the improvement effects of oral administration of polysaccharides isolated from Saururus chinensis on a DSS-induced ulcerative colitis mouse model were evaluated.To achieve this,S.chinensis-derived crude polysaccharides(SCP)were extracted using hot water extraction and ethanol precipitation.SCP is composed primarily of eight monosaccharides,including galacturonic acid(46.6%),galactose(10.4%),arabinose(5.6%),and rhamnose(3.8%),that are typical sugar components of pectic polysaccharides.Oral administration of SCP recovered body weight loss and significantly alleviated various ulcerative colitis symptoms such as stool consistency,blood in stool,and blood in the anus.Additionally,the DSS-treated group that exhibited a decrease in colon length,and SCP treatment significantly reduced colon length shortening in a dose-dependent manner.Levels of inflammatory factors such as interleukin-6,tumor necrosis factor-α,and myeloperoxidase were lower in the SCP-treated groups than they were in the DSS-treated group.In contrast,Alcian blue-periodic acid Schiff staining revealed that SCP treatment effectively maintained the amount of mucus and goblet cells in the intestinal lumen compared to that in the DSS-treated group.The short-chain fatty acid content decreased by DSS administration was recovered through oral administration of SCP,and the amount of butyric acid was significantly improved.Consequently,the aforementioned results suggest that SCP isolated from S.chinensis is a potent candidate for use as a new health functional food and pharmaceutical ingredient for colitis treatment.展开更多
利用傅里叶近红外光谱(FT-NIRS)测定了苹果的硬度。通过使用几种基于遗传算法和间隔偏最小二乘法的特征波长选取方法,包括动态向后间隔偏最小二乘(dynamic backward version of interval PLS,dynamic biPLS)、动态向后间隔偏最小二乘结...利用傅里叶近红外光谱(FT-NIRS)测定了苹果的硬度。通过使用几种基于遗传算法和间隔偏最小二乘法的特征波长选取方法,包括动态向后间隔偏最小二乘(dynamic backward version of interval PLS,dynamic biPLS)、动态向后间隔偏最小二乘结合遗传算法(dynamic biPLS & GA-PLS)和反复的遗传算法(iterative GA-PLS),分析了苹果硬度的特征波长。结果表明,运用遗传算法和间隔偏最小二乘选择特征波长后,不但可以降低模型的复杂度,同时能够达到提高模型预测精度的效果。在此基础上,研究分析了苹果硬度特征波长的物理化学意义。由于果胶是在苹果成熟过程中一种和硬度有很大关联的物质,通过比较苹果硬度的特征波长和果胶的特征吸收峰,发现两者具有有很好的一致性。因此,采用遗传算法和间隔偏最小二乘法得到的苹果硬度的特征波长能够反映果胶的吸收信息,从而解释了近红外技术检测苹果硬度的机理。展开更多
基金supported by National Natural Science Foundation of China(Grant No.81360603)Natural Science Foundation of Hainan Province(Grant No.813215)
文摘Objective:To investigate the effects of pectic polysaccharides extracted from Rauwolfia verticillata(Lour.) Baill.var.hainanensis Tsiang on an experimental murine colitis model.Methods:Experimental colitis was induced by dextran sulfate sodium(DSS),and mice were divided into 4 groups:control.DSS alone.DSS plus SASP,DSS plus pectic polysaccharides.The disease activity index(DAI) and histological score were observed.The tumor necrosis factor(TNF)-α and interleukin(IL)-17 levels were measured by enzyme-linked immunosorbent assay.I κ B and NF-κB p65 expression were assessed by western blot analysis.Myeloperoxidase(MPO) activity was determined by using MPO assay kit.Re.sults:Administration of pectic polysaccharides significantly reduced the severity of DSS-induced colitis as assessed by DAT and histological score,and resulted in down regulation of MPO activity and NF-κB p65 expression and subsequent degradation of IκB protein,strikingly reduced the production of TNF-α and IL-17.Conclusions:Pectic polysaccharides extracted from Rauvolfia verticillata(Lour.)Baill.var.hainanensis Tsiang exerts beneficial effects in experimental colitis and may therefore provide a useful therapeutic approach for the treatment of UC.
文摘AIM: To investigate H+, K+-ATPase inhibition, anti-H pylori , antioxidant, and the in vivo antiulcer potential of a pectic polysaccharide from Swallow root (Decalepis hamiltonii; SRPP). METHODS: SRPP, with known sugar composition [rhamnose: arabinose: xylose: galactose in the ratio of 16:50:2:32 (w/w), with 141 mg/g of uronic acid] was examined for anti-ulcer potency in vivo against swim/ ethanol stress-induction in animal models. Ulcer index, antioxidant/antioxidant enzymes, H+, K+-ATPase and gastric mucin levels were determined to assess the anti- ulcer potency. Anti-H pylori activity was also determined by viable colony count and electron microscopic studies. RESULTS: SRPP, containing phenolics at 0.12 g GAE/g, prevented stress-induced gastric ulcers in animal models by 80%-85%. Down regulation of gastric mucin 2-3 fold, antioxidant/antioxidant enzymes and upregulation of 3 fold of H+, K+-ATPase in ulcerous animals were normalized upon treatment with SRPP. Histopathological analysis revealed protection to the disrupted gastric mucosal layer and epithelial glands. SRPP also inhibited H+, K+-ATPase in vitro, at an IC50 of 77 μg/mL as opposed to that of 19.3 μg/mL of Lansoprazole and H pylori growth at Minimum Inhibitory Concentration (MIC) of 150 μg/mL. In addition, free radical scavenging (IC50-40 μg/mL) and reducing power (3200 U/g) activities were also observed. CONCLUSION: SRPP, with defined sugar composition and phenolics, exhibited multi-potent free radical scavenging, antioxidant, anti-H pylori, inhibition of H+, K+-ATPase and gastric mucosal protective activities. In addition, SRPP is non-toxic as opposed to other known anti-ulcer drugs, and therefore may be employed as a potential alternative for ulcer management.
基金the Project of National Natural Science Foundation of China(32202071)Zhejiang Basic Public Welfare Research Project(LQ22C200003)+1 种基金Zhejiang Province Key R&D Project(2021C02001)the Key Research and Development Program of Ningxia Province(2022BBF02006),China.
文摘Background and objectives:High-pressure processing(HPP)is a promising assistive method to extract pectic polysaccharides with high rhamnogalacturonan I(RG-I)domain and berries are sources of such pectic polysaccharides.This study extracts pectic polysaccharides from goji berry,raspberry,and cranberry,examines how HPP influences the pectic polysaccharide structure of three berries,and provides a basis for the extraction and modification of pectic polysaccharides with specific structure and bioactivity.Materials and methods:An orthogonal test was performed to optimize the HPP-assisted alkali method to extract the high yield and high RG-I content pectic polysaccharides from three berries.Structural information of pectic polysaccharides extracted by the HPP method and conventional methods were compared from the perspectives of monosaccharide composition,molecular weight,Fourier transform infrared spectroscopy,andnuclearmagneticresonancespectroscopy.Results:For raspberry,the optimal conditions consisted of a pressure of 50o MPa,a pH of 13,and a pressure-holding time of 12 min,while the optimal conditions for goji berry and cranberry were both 40o MPa,pH 13,and 15 min.Under the optimal conditions,the yields for goji berry,raspberry,and cranberry were 10.49%,16.63%,and 17.52%,respectively,and RG-l contents were 81.85%,83.30%,and 63.22%,respectively.HPP showed an effect to degrade homogalacturonan backbones and side chains and increase the RG-I content to some extent.Conclusion:HPP-assisted alkali method was revealed to be an efficient method to extract high RG-I content pectic polysaccharides,especially for cranberry,and was a potential method to modify pectic polysaccharide structure in a certain way.
基金supported by the grant from the China Agricultural Research System(CARS-35)the grant from Science and Technology Support Project of Sichuan Province(2016NYZ0052 and 2016NZ0006)the fund from the research program of“Sheng Yang”students’association(B2016010)
文摘As a kind of green additive,pectic oligosaccharide(POS) may regulate some physiological functions of animals,such as gut health,antioxidant capacity,immunity and lipid metabolism.This study aimed to identify whether POS administration can improve maternal reproduction,and to determine the possible metabolism.A total of 48 pregnant Wistar rats randomly allotted into 2 groups,and each group was fed a diet supplemented with 0 or 800 mg/kg of POS.Pectic oligosaccharide administration increased rat born number(P <0.05),did not affect rat embryo number on d 7 of gestation,but increased rat fetus number on d 14 of gestation(P <0.05).On d 14 of gestation,POS treatment improved Lactobacillus and Bifidobacterium populations and volatile fatty acid concentrations of cecal digesta(P <0.05),hormone(progesterone and nitric oxide) and cytokine(interleukin 2) concentrations of serum(P <0.05),and antioxidant capacity of serum(increased total antioxidant capacity and decreased malondialdehyde) and placenta(increased total superoxide dismutase,decreased malondialdehyde)(P <0.05) in pregnant rats.These results suggest that POS administration improved rat reproduction via decreasing fetus loss in middle gestation.This was due to the increased volatile fatty acid concentrations in rat gut improving hormone and inflammatory-cytokine productions,and antioxidant capacity.
基金supported by the National Natural Science Foundation of China(No.32072164)Natural Science Foundation of Jiangsu Province(No.BK20210458)+1 种基金the National Key Research and Development Program of China(No.2020YFC1606804)the Postdoctoral Research Funding Plan of China(No.2019M651710).
文摘The aim of this study was to continuously hydrolyze mango peel pectin,an agricultural waste,into pectic oligosaccharides(POS)with antibacterial activity.A packed-bed reactor(PBR)with immobilized polygalacturonase was designed,and the effects of the process conditions(flow rate,substrate concentration,and enzyme amount)were evaluated.The optimal conditions were obtained using response surface analysis,and a stable POS production with a yield of 94.56%was achieved.The PBR retained 82%of its initial activity after continuous operation for 72 h.The POS products exhibited good antibacterial activity against E.coli,S.aureus,B.subtilis,and S.typhimurium.The destructive effects of POS on the membrane system integrity were observed via scanning electron microscopy and the leakage of intracellular nucleic acids.Thus,the PBR is a powerful tool for continuous POS production,and hence,it can be applied as an alternative to chemical preservatives in the food industry.
文摘In the last years, sugar beet pectins have been the subject of several investigations involving extraction methodologies, chemical composition and functional properties. The structure of pectins, which depends on the extraction method, is decisive in their capacity to induce apoptosis on several cancer cell lines like colon, prostate and breast. In this work, sugar beet pectin extraction was performed in the following steps： lipid extraction with hexane, removal of soluble complex carbohydrates and proteins, and enzymatic treatment with amyloglucosidase, protease, and pectinase. The enzymatic treatment was carried out with Rohapect DA6L under the following conditions： 50℃, pH 4.0, 2% enzyme/substrate （E/S） ratio, 15 h, and a solid to liquid ratio of 1 ： 10. The pectic extract showed a degree of polymerization （DP） profile of 55.8% with DP≥7; 4.9% with DP6; 5.8% between DP2 and DP6 ; 4.7% with DP2; and 28.8% with DP1. The pectic extract was examined for its antiproliferative activity on the MCF-7 breast cancer cell line. At a concentration range of 12.5-25 mg/mL the pectic extract killed 80.6% of the cells, exhibiting a higher antiproliferative activity than 4-hydroxytamoxifen （4- OHT）, a classical anticancer drug, which killed 56.5% of the cells.
基金supported by Basic Science Research Program through the National Research Foundation of Korea(NRF)funded by the Ministry of Education(No.2018R1D1A1B07048439).
文摘The present study was carried out to evaluate the effects of different concentrations of pectic oligosaccharide(POS)on the physicochemical properties of Lactobacillus bulgaricus(L.bulgaricus)loaded-synbiotic hydrogel beads and the survival of L.bulgaricus.Low methoxyl(LM)pectin-resistant starch(RS)hydrogel beads,named RS beads,were coated/filled with different concentrations of POS:0.8%(RS-POS0.8 beads),1.0%(RS-POS1.0 beads),and 1.2%(RS-POS1.2 beads).POS treatment resulted in smooth beads with less surface dent by hydrogen bonding between RS beads and POS,which was confirmed by SEM and XRD analyses.The encapsulation efficiency of RS beads and RS-POS beads ranged from 90.40%to 92.03%.In simulated gastric fluid(SGF)and bile salt solution,free L.bulgaricus completely died but L.bulgaricus encapsulated in RS-POS1.2 beads only slightly decreased.This indicates that RS-POS1.2 beads exhibited higher protective effects on L.bulgaricus under SGF condition and bile salts.Furthermore,considering the relatively higher storage stability of L.bulgaricus loaded in RS-POS1.2 beads,the present results suggest that RS beads coated/filled with POS could be used for synbiotic encapsulation,protection,and delivery of probiotics.
基金supported by the PharmacoBio Assistantship 2019supported by the Basic Science Research Program through the National Research Foundation of Korea(NRF)funded by the Ministry of Education(NRF-2020R1F1A1070996).
文摘In this study,the improvement effects of oral administration of polysaccharides isolated from Saururus chinensis on a DSS-induced ulcerative colitis mouse model were evaluated.To achieve this,S.chinensis-derived crude polysaccharides(SCP)were extracted using hot water extraction and ethanol precipitation.SCP is composed primarily of eight monosaccharides,including galacturonic acid(46.6%),galactose(10.4%),arabinose(5.6%),and rhamnose(3.8%),that are typical sugar components of pectic polysaccharides.Oral administration of SCP recovered body weight loss and significantly alleviated various ulcerative colitis symptoms such as stool consistency,blood in stool,and blood in the anus.Additionally,the DSS-treated group that exhibited a decrease in colon length,and SCP treatment significantly reduced colon length shortening in a dose-dependent manner.Levels of inflammatory factors such as interleukin-6,tumor necrosis factor-α,and myeloperoxidase were lower in the SCP-treated groups than they were in the DSS-treated group.In contrast,Alcian blue-periodic acid Schiff staining revealed that SCP treatment effectively maintained the amount of mucus and goblet cells in the intestinal lumen compared to that in the DSS-treated group.The short-chain fatty acid content decreased by DSS administration was recovered through oral administration of SCP,and the amount of butyric acid was significantly improved.Consequently,the aforementioned results suggest that SCP isolated from S.chinensis is a potent candidate for use as a new health functional food and pharmaceutical ingredient for colitis treatment.
