目的研究敲减PTGS2对成纤维细胞全基因组表达谱的影响,在基因水平上探索防治瘢痕疙瘩的新途径。方法运用RNAi干扰正常皮肤成纤维细胞前列腺素内过氧化物合酶2(PTGS2)基因的表达,利用real time RT-PCR验证siRNA沉默效果;应用全基因组芯...目的研究敲减PTGS2对成纤维细胞全基因组表达谱的影响,在基因水平上探索防治瘢痕疙瘩的新途径。方法运用RNAi干扰正常皮肤成纤维细胞前列腺素内过氧化物合酶2(PTGS2)基因的表达,利用real time RT-PCR验证siRNA沉默效果;应用全基因组芯片检测基因表达谱变化。结果成纤维细胞的PTGS2基因经siRNA干扰后,其mRNA表达水平明显下调;全基因组芯片表达谱检测到的差异表达基因,按1.5倍差异共189个(115个上调,74个下调),按2倍差异共14个(9个上调,5个下调);基因表达谱的变化与瘢痕疙瘩基因表达谱的变化相吻合,可能促使正常皮肤成纤维细胞向瘢痕疙瘩方向进展。结论检测到与PTGS2基因相关的、在瘢痕疙瘩形成中可能共同发挥作用的相关基因,证明PTGS2与瘢痕疙瘩的发病机制有着密切的关系,为治疗瘢痕疙瘩提供了一个潜在的候选靶点。展开更多
[Objectives]To predict core targets and pathways of flavonoids from Scutellaria baicalensis against PD via network pharmacology.[Methods]Network pharmacology was employed to predict targets of six flavonoids(baicalein...[Objectives]To predict core targets and pathways of flavonoids from Scutellaria baicalensis against PD via network pharmacology.[Methods]Network pharmacology was employed to predict targets of six flavonoids(baicalein,baicalin,chrysin,wogonin,wogonoside,oroxylin A)from S.baicalensis.PD-related targets were screened from DrugBank,DisGeNET,GeneCards,and NCBI databases.Compound-target-disease networks and protein-protein interaction(PPI)networks were constructed.Functional enrichment analysis(GO/KEGG)was performed via Metascape.Molecular docking(Autodock Vina)validated ligand-target binding affinities.[Results]Intersection analysis identified 18 pivotal targets from 148 compound targets and 18 PD-associated targets.PPI network analysis revealed PTGS 2,ESR 1,TNF,and ABCB 1 as core targets(degree>6).KEGG enrichment highlighted ovarian steroidogenesis(hsa04913)and ABC transporters.Molecular docking confirmed robust binding between flavonoids and PTGS 2(binding energy<-5 kcal/mol;baicalin:-13.2).[Conclusions]Flavonoids synergistically target PTGS 2/ESR 1-mediated prostaglandin synthesis and hormonal pathways.展开更多
文摘目的研究敲减PTGS2对成纤维细胞全基因组表达谱的影响,在基因水平上探索防治瘢痕疙瘩的新途径。方法运用RNAi干扰正常皮肤成纤维细胞前列腺素内过氧化物合酶2(PTGS2)基因的表达,利用real time RT-PCR验证siRNA沉默效果;应用全基因组芯片检测基因表达谱变化。结果成纤维细胞的PTGS2基因经siRNA干扰后,其mRNA表达水平明显下调;全基因组芯片表达谱检测到的差异表达基因,按1.5倍差异共189个(115个上调,74个下调),按2倍差异共14个(9个上调,5个下调);基因表达谱的变化与瘢痕疙瘩基因表达谱的变化相吻合,可能促使正常皮肤成纤维细胞向瘢痕疙瘩方向进展。结论检测到与PTGS2基因相关的、在瘢痕疙瘩形成中可能共同发挥作用的相关基因,证明PTGS2与瘢痕疙瘩的发病机制有着密切的关系,为治疗瘢痕疙瘩提供了一个潜在的候选靶点。
基金Supported by Key Research Project of Wuhan Health and Wellness Commission(WZ20A10)Hubei Shizhen Talent Program Research Project(Notice No.256[2024]of Hubei Provincial Health Commission).
文摘[Objectives]To predict core targets and pathways of flavonoids from Scutellaria baicalensis against PD via network pharmacology.[Methods]Network pharmacology was employed to predict targets of six flavonoids(baicalein,baicalin,chrysin,wogonin,wogonoside,oroxylin A)from S.baicalensis.PD-related targets were screened from DrugBank,DisGeNET,GeneCards,and NCBI databases.Compound-target-disease networks and protein-protein interaction(PPI)networks were constructed.Functional enrichment analysis(GO/KEGG)was performed via Metascape.Molecular docking(Autodock Vina)validated ligand-target binding affinities.[Results]Intersection analysis identified 18 pivotal targets from 148 compound targets and 18 PD-associated targets.PPI network analysis revealed PTGS 2,ESR 1,TNF,and ABCB 1 as core targets(degree>6).KEGG enrichment highlighted ovarian steroidogenesis(hsa04913)and ABC transporters.Molecular docking confirmed robust binding between flavonoids and PTGS 2(binding energy<-5 kcal/mol;baicalin:-13.2).[Conclusions]Flavonoids synergistically target PTGS 2/ESR 1-mediated prostaglandin synthesis and hormonal pathways.
