摘要
【目的】为揭示PTGS2基因多态性与母猪繁殖性状的关联。【方法】采用PCR产物直接测序法对401头大白母猪PTGS2基因第3内含子的SNP位点进行检测,结合1 786窝母猪繁殖性能记录,采用最小二乘模型分析了各多态位点基因型及其单倍型组合对5个繁殖性状的影响。【结果】大白猪PTGS2基因第3内含子区域存在3个紧密连锁的SNP位点:G227A、A392C和T409C,分别以等位基因G、A、T及其纯合子(GG、AA和TT)、GAT单倍型及GAT/GAT组合的频率最高,各位点杂合度在0.320 9～0.353 6之间,遗传多样性较为丰富;G227A位点的GA基因型、A392C位点的AC基因型、T409C位点的TC基因型及GAT单倍型具有显著提高母猪总产仔数、产活仔数和仔猪21日龄成活数的效应(P<0.05或P<0.01),AA、CC和CC基因型及GCC/GCC单倍型组合具有显著提高仔猪21日龄窝重的效应(P<0.05或P<0.01)。【结论】研究结果丰富了猪PTGS2基因的SNP标记,初步证实PTGS2基因对母猪繁殖性状有显著影响,但其具体效应可能会因品种(或猪群)及具体性状的不同而有一定差异。
[Purpose]The aim of the present study was to reveal the association of the polymorphisms of prostaglandin-endoperoxide synthase 2(PTGS2)gene with sow reproductive traits.[Method]The single nucleotide polymorphisms(SNPs)within the intron 3 of PTGS2 gene were detected by PCR product directed sequencing in401Large White(LW)sows.Combing the data of1786litters reproductive performance,the effects of each locus and haplotype combinations for multiple loci on five reproductive traits were analyzed by least square model.[Results]Three closely linked SNPs,G227A,A392C and T409C were identified within the intron3of PTGS2gene in LW pigs.The highest frequency was presented for the alleles G,A,T,the homozygous genotypes GG,AA,TT,haplotype GAT and the combination GAT/GAT;the relative abundant genetic diversity was showed on the three loci,and the heterozygosities of the loci were ranged from0.3209 to 0.3536 in LW pigs.Sows with genotype GA at G227A,AC at A392C,TC at T409C locus,and haplotype GAT had higher total number born(TNB),number of piglets born alive(NBA)and number of alive piglets at21st day than those of the other genotypes and haplotypes significantly(P<0.05or P<0.01);the genotype AA,CC,CC of the three loci and haplotype combination GCC/GCC had significant increasing effects on the litter weights at 21st day(P<0.05or P<0.01).[Conclusion]The results further enriched the SNP markers of porcine PTGS2gene,and preliminary confirmed that the PTGS2gene was significantly associated with reproductive traits of sows,but the specific effects might be variety on different traits and in different breeds or populations.
作者
高玉森
董新星
张燕林
兰国湘
计春律
严达伟
苟潇
李明丽
鲁绍雄
GAO Yusen;DONG Xinxing;ZHANG Yanlin;LAN Guoxiang;JI Chunlyu;YAN Dawei;GOU Xiao;LI Mingli;LU Shaoxiong(College of Animal Science and Technology,Yunnan Agricultural University,Kunming 650201,China;Yunnan Key Laboratory of Animal Nutrition and Feed,Kunming 650201,China;Yunnan Huijia Breeding Co.Ltd.,Kunming 650300,China)
出处
《云南农业大学学报（自然科学版）》
CSCD
北大核心
2018年第6期1068-1074,共7页
Journal of Yunnan Agricultural University:Natural Science
基金
NSFC-云南联合基金项目(U1402266)
云南省生猪产业技术体系建设项目(2018KJTX0013)
关键词
大白猪
PTGS2基因
内含子3
单核苷酸多态性
繁殖性状
Large White pig
prostaglandin-endoperoxide synthase2(PTGS2)gene
intron3
single nucleotide polymorphism(SNP)
reproductive trait
