Abstract： Aim To investigate the role of CKLF1 in SH-SYSY cell migration and its molecular regulatory mecha- nism. Methods SH-SYSY cells were stimulated with CKLF1 for 0.5, 2, 8 and 24 h, respectively. The migration ...Abstract： Aim To investigate the role of CKLF1 in SH-SYSY cell migration and its molecular regulatory mecha- nism. Methods SH-SYSY cells were stimulated with CKLF1 for 0.5, 2, 8 and 24 h, respectively. The migration distance and the percentage of migration cells were recorded by CELLocate analysis. The phosphorylation of focal adhesion kinase （FAK） at Tyr-397 site was detected by Western blot analysis. By chemotaxis assays, we confirmed the chemotaxis of CKLF1. Furthermore, FAK inhibitor PF-573228 and PLCγ inhibitor U73122 were used for the research of molecular regulatory mechanisms involved. Results CKLF1 promoted cell migration and induced a strong increase in the phosphorylation level of FAK - pY397, which were significantly attenuated by the presence of U73122 （a specific inhibitor for PLCγ）. In addition, the chemotaxis of CKLF1 was obviously blocked by the FAK inhibitor PF-573228. Conclusions CKLF1 induces SH-SYSY cell migration via PLCT/FAK signaling path- way.展开更多
针对触摸屏监控系统不能满足大中型立体仓库对数据进行存储和处理的功能需求,在Visual Studio 2019集成开发环境中,采用C#语言开发一套立体仓库上位机控制系统。以多线程的方式实时读取库位信息;以S7-1200 PLC作为主控制器,设计产品的...针对触摸屏监控系统不能满足大中型立体仓库对数据进行存储和处理的功能需求,在Visual Studio 2019集成开发环境中,采用C#语言开发一套立体仓库上位机控制系统。以多线程的方式实时读取库位信息;以S7-1200 PLC作为主控制器,设计产品的自动入库和自动出库程序流程,采用SCL语言设计了库位先进先出的控制程序。C#和S7-1200 PLC之间采用S7通信的方式控制立体仓库的出入库操作和库位信息采集,3年的现场运行情况表明,整个系统能在上位机上对立体仓库进行手动控制和自动控制,能精确快速地进行入库出库操作,运行平稳,上位机上能正确实时显示库位信息,达到了预期的结果。展开更多
文摘Abstract： Aim To investigate the role of CKLF1 in SH-SYSY cell migration and its molecular regulatory mecha- nism. Methods SH-SYSY cells were stimulated with CKLF1 for 0.5, 2, 8 and 24 h, respectively. The migration distance and the percentage of migration cells were recorded by CELLocate analysis. The phosphorylation of focal adhesion kinase （FAK） at Tyr-397 site was detected by Western blot analysis. By chemotaxis assays, we confirmed the chemotaxis of CKLF1. Furthermore, FAK inhibitor PF-573228 and PLCγ inhibitor U73122 were used for the research of molecular regulatory mechanisms involved. Results CKLF1 promoted cell migration and induced a strong increase in the phosphorylation level of FAK - pY397, which were significantly attenuated by the presence of U73122 （a specific inhibitor for PLCγ）. In addition, the chemotaxis of CKLF1 was obviously blocked by the FAK inhibitor PF-573228. Conclusions CKLF1 induces SH-SYSY cell migration via PLCT/FAK signaling path- way.
文摘针对触摸屏监控系统不能满足大中型立体仓库对数据进行存储和处理的功能需求,在Visual Studio 2019集成开发环境中,采用C#语言开发一套立体仓库上位机控制系统。以多线程的方式实时读取库位信息;以S7-1200 PLC作为主控制器,设计产品的自动入库和自动出库程序流程,采用SCL语言设计了库位先进先出的控制程序。C#和S7-1200 PLC之间采用S7通信的方式控制立体仓库的出入库操作和库位信息采集,3年的现场运行情况表明,整个系统能在上位机上对立体仓库进行手动控制和自动控制,能精确快速地进行入库出库操作,运行平稳,上位机上能正确实时显示库位信息,达到了预期的结果。
