Microglia,the resident monocyte of the central nervous system,play a crucial role in the response to spinal cord injury.However,the precise mechanism remains unclear.To investigate the molecular mechanisms by which mi...Microglia,the resident monocyte of the central nervous system,play a crucial role in the response to spinal cord injury.However,the precise mechanism remains unclear.To investigate the molecular mechanisms by which microglia regulate the neuroinflammatory response to spinal cord injury,we performed single-cell RNA sequencing dataset analysis,focusing on changes in microglial subpopulations.We found that the MG1 subpopulation emerged in the acute/subacute phase of spinal cord injury and expressed genes related to cell pyroptosis,sphingomyelin metabolism,and neuroinflammation at high levels.Subsequently,we established a mouse model of contusive injury and performed intrathecal injection of siRNA and molecular inhibitors to validate the role of ceramide synthase 5 in the neuroinflammatory responses and pyroptosis after spinal cord injury.Finally,we established a PC12-BV2 cell co-culture system and found that ceramide synthase 5 and pyroptosis-associated proteins were highly expressed to induce the apoptosis of neuron cells.Inhibiting ceramide synthase 5 expression in a mouse model of spinal cord injury effectively reduced pyroptosis.Furthermore,ceramide synthase 5-induced pyroptosis was dependent on activation of the NLRP3 signaling pathway.Inhibiting ceramide synthase 5 expression in microglia in vivo reduced neuronal apoptosis and promoted recovery of neurological function.Pla2g7 formed a“bridge”between sphingolipid metabolism and ceramide synthase 5-mediated cell death by inhibiting the NLRP3 signaling pathway.Collectively,these findings suggest that inhibiting ceramide synthase 5 expression in microglia after spinal cord injury effectively suppressed microglial pyroptosis mediated by NLRP3,thereby exerting neuroprotective effects.展开更多
Objective:To investigate the potential targets and mechanisms of Draconis Sanguis(DS),a valuable traditional Chinese medicine derived from the resin of the palm tree Daemonorops draco Bl(D.Sanguis,Xue Jie),in the trea...Objective:To investigate the potential targets and mechanisms of Draconis Sanguis(DS),a valuable traditional Chinese medicine derived from the resin of the palm tree Daemonorops draco Bl(D.Sanguis,Xue Jie),in the treatment of myocardial infarction(MI).Methods:We explored the potential mechanisms of DS in the treatment of MI using network pharmacology,bioinformatic techniques,and transcriptomic analysis,followed by validation through in vivo and in vitro experiments.Results:Network pharmacology and bioinformatic analyses identified five genes(Fpr1,Glul,Mme,Mmp9,and Pla2g7)as potential targets for MI treatment.Moreover,DS significantly ameliorated cardiac function,inflammatory responses,and MI-induced myocardial fibrosis in vivo.Transcriptomic and bioinformatic analyses identified Pla2g7 as the most critical target in the DS treatment of MI.Molecular docking revealed that the key active ingredient in DS has a strong affinity for this gene.Furthermore,DS reduced the expression of Pla2g7(P=.0009),NLRP3(P=.003),interleukin-18(P<.001),and interleukin-1b(P=.004)mRNAs in vivo.Conclusions:The results indicate that DS can downregulate the expression of Pla2g7 and reduce the inflammatory response.This demonstrates the potential therapeutic target of DS and the mechanism underlying its cardioprotective effects.展开更多
目的:探究清肾汤联合他克莫司胶囊、泼尼松对脾肾两虚湿热内蕴型膜性肾病患者的治疗效果,特别是对血尿、血脂、肾功能和磷脂酶A2受体(PLA2R)滴度水平的影响。方法:选取2022年1月—2024年3月徐州市中医院收治的82例确诊为脾肾两虚湿热内...目的:探究清肾汤联合他克莫司胶囊、泼尼松对脾肾两虚湿热内蕴型膜性肾病患者的治疗效果,特别是对血尿、血脂、肾功能和磷脂酶A2受体(PLA2R)滴度水平的影响。方法:选取2022年1月—2024年3月徐州市中医院收治的82例确诊为脾肾两虚湿热内蕴型膜性肾病患者,采用倾向性评分匹配分为对照组和研究组,每组41例。对照组接受他克莫司胶囊、泼尼松治疗,研究组在此基础上加用清肾汤治疗,疗程为3个月。比较两组在治疗前后临床疗效、镜下血尿检出率、血脂指标[甘油三酯(TG)、总胆固醇(TC)]、肾功能指标[白蛋白(Alb)、24 h尿蛋白(24 h Upro)、肌酐(Scr)、尿素氮(BUN)、尿视黄醇结合蛋白(RBP)]及PLA2R滴度水平的变化,并评估不良反应发生率。结果:治疗3个月后,研究组总有效率为95.12%(39/41)高于对照组的80.49%(33/41),差异有统计学意义(P<0.05)。研究组血尿检出率为7.32%(3/41),低于对照组的21.95%(9/41),差异有统计学意义(P<0.05)。两组TG、TC水平均下降(P<0.05),且研究组降幅更大(P<0.05)。两组Alb水平均升高(P<0.05),24 h Upro、Scr、BUN、RBP、PLA2R滴度水平均降低(P<0.05),且研究组改善情况优于对照组(P<0.05)。两组患者不良反应发生率无明显差异(P>0.05)。结论:清肾汤联合他克莫司胶囊、泼尼松治疗可显著改善脾肾两虚湿热内蕴型膜性肾病患者的临床症状、血尿、血脂和肾功能,降低PLA2R滴度水平,且安全性良好。本研究支持清肾汤作为膜性肾病患者的有效辅助治疗方案。展开更多
基金supported by grants from the National Key Research and Development Program of China,No.2017YFA0105400(to LR)the Key Research and Development Program of Guangdong Province,No.2019B020236002(to LR)the National Natural Science Foundation of China,Nos.81972111(to LZ),81772349(to BL).
文摘Microglia,the resident monocyte of the central nervous system,play a crucial role in the response to spinal cord injury.However,the precise mechanism remains unclear.To investigate the molecular mechanisms by which microglia regulate the neuroinflammatory response to spinal cord injury,we performed single-cell RNA sequencing dataset analysis,focusing on changes in microglial subpopulations.We found that the MG1 subpopulation emerged in the acute/subacute phase of spinal cord injury and expressed genes related to cell pyroptosis,sphingomyelin metabolism,and neuroinflammation at high levels.Subsequently,we established a mouse model of contusive injury and performed intrathecal injection of siRNA and molecular inhibitors to validate the role of ceramide synthase 5 in the neuroinflammatory responses and pyroptosis after spinal cord injury.Finally,we established a PC12-BV2 cell co-culture system and found that ceramide synthase 5 and pyroptosis-associated proteins were highly expressed to induce the apoptosis of neuron cells.Inhibiting ceramide synthase 5 expression in a mouse model of spinal cord injury effectively reduced pyroptosis.Furthermore,ceramide synthase 5-induced pyroptosis was dependent on activation of the NLRP3 signaling pathway.Inhibiting ceramide synthase 5 expression in microglia in vivo reduced neuronal apoptosis and promoted recovery of neurological function.Pla2g7 formed a“bridge”between sphingolipid metabolism and ceramide synthase 5-mediated cell death by inhibiting the NLRP3 signaling pathway.Collectively,these findings suggest that inhibiting ceramide synthase 5 expression in microglia after spinal cord injury effectively suppressed microglial pyroptosis mediated by NLRP3,thereby exerting neuroprotective effects.
基金the National Natural Science Foundation of China(82222075).
文摘Objective:To investigate the potential targets and mechanisms of Draconis Sanguis(DS),a valuable traditional Chinese medicine derived from the resin of the palm tree Daemonorops draco Bl(D.Sanguis,Xue Jie),in the treatment of myocardial infarction(MI).Methods:We explored the potential mechanisms of DS in the treatment of MI using network pharmacology,bioinformatic techniques,and transcriptomic analysis,followed by validation through in vivo and in vitro experiments.Results:Network pharmacology and bioinformatic analyses identified five genes(Fpr1,Glul,Mme,Mmp9,and Pla2g7)as potential targets for MI treatment.Moreover,DS significantly ameliorated cardiac function,inflammatory responses,and MI-induced myocardial fibrosis in vivo.Transcriptomic and bioinformatic analyses identified Pla2g7 as the most critical target in the DS treatment of MI.Molecular docking revealed that the key active ingredient in DS has a strong affinity for this gene.Furthermore,DS reduced the expression of Pla2g7(P=.0009),NLRP3(P=.003),interleukin-18(P<.001),and interleukin-1b(P=.004)mRNAs in vivo.Conclusions:The results indicate that DS can downregulate the expression of Pla2g7 and reduce the inflammatory response.This demonstrates the potential therapeutic target of DS and the mechanism underlying its cardioprotective effects.
文摘目的:探究清肾汤联合他克莫司胶囊、泼尼松对脾肾两虚湿热内蕴型膜性肾病患者的治疗效果,特别是对血尿、血脂、肾功能和磷脂酶A2受体(PLA2R)滴度水平的影响。方法:选取2022年1月—2024年3月徐州市中医院收治的82例确诊为脾肾两虚湿热内蕴型膜性肾病患者,采用倾向性评分匹配分为对照组和研究组,每组41例。对照组接受他克莫司胶囊、泼尼松治疗,研究组在此基础上加用清肾汤治疗,疗程为3个月。比较两组在治疗前后临床疗效、镜下血尿检出率、血脂指标[甘油三酯(TG)、总胆固醇(TC)]、肾功能指标[白蛋白(Alb)、24 h尿蛋白(24 h Upro)、肌酐(Scr)、尿素氮(BUN)、尿视黄醇结合蛋白(RBP)]及PLA2R滴度水平的变化,并评估不良反应发生率。结果:治疗3个月后,研究组总有效率为95.12%(39/41)高于对照组的80.49%(33/41),差异有统计学意义(P<0.05)。研究组血尿检出率为7.32%(3/41),低于对照组的21.95%(9/41),差异有统计学意义(P<0.05)。两组TG、TC水平均下降(P<0.05),且研究组降幅更大(P<0.05)。两组Alb水平均升高(P<0.05),24 h Upro、Scr、BUN、RBP、PLA2R滴度水平均降低(P<0.05),且研究组改善情况优于对照组(P<0.05)。两组患者不良反应发生率无明显差异(P>0.05)。结论:清肾汤联合他克莫司胶囊、泼尼松治疗可显著改善脾肾两虚湿热内蕴型膜性肾病患者的临床症状、血尿、血脂和肾功能,降低PLA2R滴度水平,且安全性良好。本研究支持清肾汤作为膜性肾病患者的有效辅助治疗方案。
