PII proteins are a protein family important to signal transduction in bacteria and plants. PII plays a critical role in regulation of carbon and nitrogen metabolism in cyanobacteria. Through conformation change and co...PII proteins are a protein family important to signal transduction in bacteria and plants. PII plays a critical role in regulation of carbon and nitrogen metabolism in cyanobacteria. Through conformation change and covalent modification, which are regulated by 2-oxoglutarate, PII interacts with different target proteins in response to changes of cellular energy status and carbon and nitrogen sources in cyanobacteria and regulates cellular metabolism. This article reports recent progress in PII research in cyanobacteria and discusses the mechanism of PII regulation of cellular metabolism .展开更多
The interaction between PII and NifA in A. brasilense Sp7 was investigated by using the yeast two-hybrid system. Our experimental results showed that PII directly interacted with the entire NifA protein and its N-term...The interaction between PII and NifA in A. brasilense Sp7 was investigated by using the yeast two-hybrid system. Our experimental results showed that PII directly interacted with the entire NifA protein and its N-terminal domain,but did not interact with the central domain and the C-terminal domain of NifA. No interaction happened if glnB coding for PII was frame-shift mutated. Pz, a homolog of PII, had no interation with NifA.展开更多
基金Supported by the National Science Foundation of China (Grant No.30630006)
文摘PII proteins are a protein family important to signal transduction in bacteria and plants. PII plays a critical role in regulation of carbon and nitrogen metabolism in cyanobacteria. Through conformation change and covalent modification, which are regulated by 2-oxoglutarate, PII interacts with different target proteins in response to changes of cellular energy status and carbon and nitrogen sources in cyanobacteria and regulates cellular metabolism. This article reports recent progress in PII research in cyanobacteria and discusses the mechanism of PII regulation of cellular metabolism .
基金This work was supported by the National 73Project (Grant No. 001CB108904) and the National Natural Science Foundation of China (Grant No.30070407).
文摘The interaction between PII and NifA in A. brasilense Sp7 was investigated by using the yeast two-hybrid system. Our experimental results showed that PII directly interacted with the entire NifA protein and its N-terminal domain,but did not interact with the central domain and the C-terminal domain of NifA. No interaction happened if glnB coding for PII was frame-shift mutated. Pz, a homolog of PII, had no interation with NifA.
