Silkworm pupae oil(SPO)was regarded as a good alternative to alpha-linolenic acid supplements,which contain a variety of triacylglyceride with high nutritional value.To investigate the connections between triacylglyce...Silkworm pupae oil(SPO)was regarded as a good alternative to alpha-linolenic acid supplements,which contain a variety of triacylglyceride with high nutritional value.To investigate the connections between triacylglyceride composition and lipid gastrointestinal digestive absorption profiles,isolation and bioavailability of triacylglyceride monomers from silkworm pupae oil was analyzed.In this study,two triacylglyceride monomers rich inα-linolenic acid were separated by preparative liquid chromatography,namely LnLnLn(1,2,3-α-linolenic acid triacylglyceride)and LnLnL(1,2-α-linolenic acid,3-linoleic acid triacylglyceride).The in vitro digestion in the pH-stat model results showed the digestive rate of LnLnLn was 0.4101±0.0089 s^(-1),which was significantly higher than that of SPO(0.2494±0.0108 s^(-1))(p<0.05).The in vitro absorption in the Caco-2 cells model results showed the absorptivity of LnLnLn and LnLnL were 6.40±0.19%and 6.06±0.54%,which were significantly higher than SPO(5.43±0.51%)(p<0.05).In addition,the mRNA expression levels of lipid metabolism-related proteins provide evidence for the absorption superiority of LnLnLn and LnLnL.The present work provides novel structural lipids for rapid and efficient supplementation ofα-linolenic acid.展开更多
目的探讨深低温体外循环(Extracorporeal c ircu lation,ECC)温度管理策略。方法选取15例夹层动脉瘤患者在深低温停循环下行主动脉弓部手术,ECC复温过程中采用平衡变温方法进行温度管理,用pH稳态管理酸碱平衡,合理应用药物。结果动脉血...目的探讨深低温体外循环(Extracorporeal c ircu lation,ECC)温度管理策略。方法选取15例夹层动脉瘤患者在深低温停循环下行主动脉弓部手术,ECC复温过程中采用平衡变温方法进行温度管理,用pH稳态管理酸碱平衡,合理应用药物。结果动脉血气指标PaCO2及pH值等维持在正常范围内,全组无神经系统等重要脏器并发症,无术后明显体温降低。结论深低温下采用pH稳态血气管理、平衡复温、合理应用药物等综合措施,能取得满意的临床效果。展开更多
基金supported by the Jiangsu Agricultural Science and Technology Innovation Fund(CX(20)2029)the Postgraduate Research&Practice Innovation Program of Jiangsu Province(KYCX22_3854).
文摘Silkworm pupae oil(SPO)was regarded as a good alternative to alpha-linolenic acid supplements,which contain a variety of triacylglyceride with high nutritional value.To investigate the connections between triacylglyceride composition and lipid gastrointestinal digestive absorption profiles,isolation and bioavailability of triacylglyceride monomers from silkworm pupae oil was analyzed.In this study,two triacylglyceride monomers rich inα-linolenic acid were separated by preparative liquid chromatography,namely LnLnLn(1,2,3-α-linolenic acid triacylglyceride)and LnLnL(1,2-α-linolenic acid,3-linoleic acid triacylglyceride).The in vitro digestion in the pH-stat model results showed the digestive rate of LnLnLn was 0.4101±0.0089 s^(-1),which was significantly higher than that of SPO(0.2494±0.0108 s^(-1))(p<0.05).The in vitro absorption in the Caco-2 cells model results showed the absorptivity of LnLnLn and LnLnL were 6.40±0.19%and 6.06±0.54%,which were significantly higher than SPO(5.43±0.51%)(p<0.05).In addition,the mRNA expression levels of lipid metabolism-related proteins provide evidence for the absorption superiority of LnLnLn and LnLnL.The present work provides novel structural lipids for rapid and efficient supplementation ofα-linolenic acid.
文摘目的探讨深低温体外循环(Extracorporeal c ircu lation,ECC)温度管理策略。方法选取15例夹层动脉瘤患者在深低温停循环下行主动脉弓部手术,ECC复温过程中采用平衡变温方法进行温度管理,用pH稳态管理酸碱平衡,合理应用药物。结果动脉血气指标PaCO2及pH值等维持在正常范围内,全组无神经系统等重要脏器并发症,无术后明显体温降低。结论深低温下采用pH稳态血气管理、平衡复温、合理应用药物等综合措施,能取得满意的临床效果。
