The direct evidence of the location of the mosquito-larvicidal gene of Bacillus sphaericus10 (isolated from Jiangsu Province of China) on megaplasmid pFW1 was given by molecularcloning. The clone (pFL109) containing t...The direct evidence of the location of the mosquito-larvicidal gene of Bacillus sphaericus10 (isolated from Jiangsu Province of China) on megaplasmid pFW1 was given by molecularcloning. The clone (pFL109) containing the 1.4 kb HindⅢ DNA fragment from pFW1expressed the mosquito-larvicidal toxin protein (43 kD). The location of the gene codingfor the 43 kD toxin protein within the Xhol B fragment on the restriction map of pFW1 wasconfirmed by Southern blotting using the 1.4 kb HindⅢ DNA fragment as a probe. The non-toxic strains of Bacillus sphaericus were revealed to be 43 kD toxin gene dele-tion mutants by Southern and Western analyses. The 1.4 kb HindⅢ fragment of pFL109 can be used as a probe for differentiating thenon-toxic strains of Bacillus sphaericus from the toxic ones.展开更多
文摘The direct evidence of the location of the mosquito-larvicidal gene of Bacillus sphaericus10 (isolated from Jiangsu Province of China) on megaplasmid pFW1 was given by molecularcloning. The clone (pFL109) containing the 1.4 kb HindⅢ DNA fragment from pFW1expressed the mosquito-larvicidal toxin protein (43 kD). The location of the gene codingfor the 43 kD toxin protein within the Xhol B fragment on the restriction map of pFW1 wasconfirmed by Southern blotting using the 1.4 kb HindⅢ DNA fragment as a probe. The non-toxic strains of Bacillus sphaericus were revealed to be 43 kD toxin gene dele-tion mutants by Southern and Western analyses. The 1.4 kb HindⅢ fragment of pFL109 can be used as a probe for differentiating thenon-toxic strains of Bacillus sphaericus from the toxic ones.
