摘要
目的:构建变异链球菌RgpAc基因敲除突变株,为进一步研究变异链球菌RgpAc基因功能做准备。方法:通过PCR扩增RgpAc基因上下游序列,分别插入到pFW5载体的2个多克隆酶切位点中,获得重组质粒。采用同源重组的方式将重组质粒转化到变异链球菌UA159中,获得RgpAc基因敲除突变株。结果:聚合酶链式反应(PCR)显示,突变株RgpAc基因已被载体完全替换。结论:成功构建变异链球菌RgpAc基因敲除突变株。
AIM： To construct a RgpAc knockout strain of Streptococcus mutans. METHODS： The up- stream and downstream sequences of RgpAc gene were amplified by polymerase chain reaction （PCR） , and were then inserted into 2 multiple clone enzyme cutting sites of pFW5 vector. The reconstructed plasmid was transformed into Streptococcus mutans UA159 by homologous recombination to get the RgpAc gene knock-out mutant. RESULTS： PCR and fluorescence quantitative PCR results showed that the RgpAc gene was replaced. CONCLUSION： Streptococcus mutans RgpAc knockout mutant strain was successfully constructed.
出处
《牙体牙髓牙周病学杂志》
CAS
北大核心
2013年第6期380-383,共4页
Chinese Journal of Conservative Dentistry
基金
国家自然科学基金项目(30672327
81100747)
