Double-stranded oligomeric nucleotide encoding PEP-1 peptides was synthesized, pro- karyotic expression pET15b-pep-1-p27mt recombinant constructed, E. coli BL21 (DE3)pLysS trans- formed and induced with IPTG to highly...Double-stranded oligomeric nucleotide encoding PEP-1 peptides was synthesized, pro- karyotic expression pET15b-pep-1-p27mt recombinant constructed, E. coli BL21 (DE3)pLysS trans- formed and induced with IPTG to highly express fusion protein PEP-1-P27mt. Fusion protein with an N-terminal His-tag could be purified by Ni2+-resin affinity chromatography and identified by SDS-PAGE and Western blotting. Cultured EC9706 cells treated with PEP-1-P27mt revealed that PEP-1-P27mt was transduced into cells after 15 min and reached maximal intracellular concentra- tions in 2 h. PEP-1-P27mt of 1 μmol/L final concentration could most strongly suppress the growth. It was suggested that PEP-1 can carry P27mt across membrane, which provides a new biological pro- tocol for using cyclin dependent kinase inhibitors p27mt in suppressing the growth of tumor cells.展开更多
本文研究了小麦（Triticum aestivum L.）和大豆（Glycine max L.）的不同光合器官中的PEP羧化酶及其相关的酶类。在这些器官中均含有PEP羧化酶,其中以大豆的荚壳和种皮及小麦的内稃中PEP羧化酶活性最高。不同绿色器官在光下或暗中均能...本文研究了小麦（Triticum aestivum L.）和大豆（Glycine max L.）的不同光合器官中的PEP羧化酶及其相关的酶类。在这些器官中均含有PEP羧化酶,其中以大豆的荚壳和种皮及小麦的内稃中PEP羧化酶活性最高。不同绿色器官在光下或暗中均能固定CO2,但是在黑暗条件下,小麦的内稃和大豆的荚壳及种皮所固定的CO2远高于叶片。此外,参与暗固定的NAD-苹果酸酶和NAD-苹果酸脱氢酶的活性,在这些器官中也最高,说明这些器官中PEP羧化酶的CO2 β-羧化作用主要在于固定呼吸作用所释放的CO2,只有少量的CO2是通过C4光合途径被固定。展开更多
钍基熔盐堆(Thorium Molten Salt Reactor-Liquid Fuel,TMSR-LF1)回路管道最高运行温度达650℃,高温服役下的管道蠕变-疲劳损伤分析及评定至关重要。目前仅ASME-BPVC-III-5-HBB规范中有适用于高温核一级管道的蠕变-疲劳损伤暂行评定方法...钍基熔盐堆(Thorium Molten Salt Reactor-Liquid Fuel,TMSR-LF1)回路管道最高运行温度达650℃,高温服役下的管道蠕变-疲劳损伤分析及评定至关重要。目前仅ASME-BPVC-III-5-HBB规范中有适用于高温核一级管道的蠕变-疲劳损伤暂行评定方法,但该方法对于复杂管道系统使用起来过于繁琐。本文旨在使用管道分析软件PepS软件实现高温核一级复杂管系的分析与结构完整性评估。首先结合管道结构在多种载荷组合作用下的截面应力状态解析解,进行管道截面应力分析及应力线性化,并将结果与有限元数值解进行对比分析,两者的误差结果基本一致。随后,利用PepS软件对TMSR-LF1回路管道进行了力学分析和结构完整性评估,结其蠕变疲劳损伤结果位于包络线以内,满足蠕变疲劳极限的要求。该研究将管道分析软件与ASME评定规范进行了有效衔接,明确了评定方法,实现了高温核一级复杂管系的蠕变疲劳评估。展开更多
基金a grant from Department of Education of Hubei Province, China (No. Q200524001).
文摘Double-stranded oligomeric nucleotide encoding PEP-1 peptides was synthesized, pro- karyotic expression pET15b-pep-1-p27mt recombinant constructed, E. coli BL21 (DE3)pLysS trans- formed and induced with IPTG to highly express fusion protein PEP-1-P27mt. Fusion protein with an N-terminal His-tag could be purified by Ni2+-resin affinity chromatography and identified by SDS-PAGE and Western blotting. Cultured EC9706 cells treated with PEP-1-P27mt revealed that PEP-1-P27mt was transduced into cells after 15 min and reached maximal intracellular concentra- tions in 2 h. PEP-1-P27mt of 1 μmol/L final concentration could most strongly suppress the growth. It was suggested that PEP-1 can carry P27mt across membrane, which provides a new biological pro- tocol for using cyclin dependent kinase inhibitors p27mt in suppressing the growth of tumor cells.
文摘钍基熔盐堆(Thorium Molten Salt Reactor-Liquid Fuel,TMSR-LF1)回路管道最高运行温度达650℃,高温服役下的管道蠕变-疲劳损伤分析及评定至关重要。目前仅ASME-BPVC-III-5-HBB规范中有适用于高温核一级管道的蠕变-疲劳损伤暂行评定方法,但该方法对于复杂管道系统使用起来过于繁琐。本文旨在使用管道分析软件PepS软件实现高温核一级复杂管系的分析与结构完整性评估。首先结合管道结构在多种载荷组合作用下的截面应力状态解析解,进行管道截面应力分析及应力线性化,并将结果与有限元数值解进行对比分析,两者的误差结果基本一致。随后,利用PepS软件对TMSR-LF1回路管道进行了力学分析和结构完整性评估,结其蠕变疲劳损伤结果位于包络线以内,满足蠕变疲劳极限的要求。该研究将管道分析软件与ASME评定规范进行了有效衔接,明确了评定方法,实现了高温核一级复杂管系的蠕变疲劳评估。
