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Influence of ultrafine cement on cement-soil in peat soil environment of Dianchi Lake
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作者 CAO Jing SUN Huafeng +5 位作者 HUANG Siyang KONG Cheng LIU Fangyi LIU Fuhua TIAN Lin ZHU Weiming 《土木与环境工程学报(中英文)》 北大核心 2025年第2期1-12,共12页
Treatment of peat soil foundation in Yunnan surrounding Dianchi and Erhai Lakes poses complex problems for engineering projects.It is insufficient to rely on ordinary cement to reinforce peat soil.In order to make the... Treatment of peat soil foundation in Yunnan surrounding Dianchi and Erhai Lakes poses complex problems for engineering projects.It is insufficient to rely on ordinary cement to reinforce peat soil.In order to make the reinforcement reliable,this experiment mixed(ultrafine cement)UFC into ordinary cement to form a composite solidify agent.This study aimed to analyze the influence of UFC proportion on the strength of cement-soil in the peat soil environment.Unconfined compressive strength(UCS)and scanning electron microscope(SEM)tests were conducted on samples soaked for 28 and 90 days,respectively.The test results show that without considering the effects of Humic Acid(HA)and Fulvic Acid(FA),incorporating UFC can significantly improve the UCS of cement-soil.The rapid hydration of the fine particles generates a large number of cementitious products,improves the cohesion of the soil skeleton,and fills the pores.However,when the proportion of UFC increases,the aggregate structure formed by a larger quantity of fine particles reduces the hydration rate and degree of cement hydration,making the UCS growth rate of cement-soil insignificant.In the peat soil environment,HA significantly weakened the UCS of cement-soil in both physical and chemical aspects.However,UFC can mitigate the adverse effect of HA on cement-soil by its small particle size,high surface energy,and solid binding ability.In addition,FA has a positive effect on the UCS of cement-soil soaked for 28 days and 90 days.The UFC addition could promote the enhancement effect of FA on cement-soil UCS.SEM test results showed that cement hydration products increased significantly with the increase of UFC proportion,and cementation between hydration products and soil particles was enhanced.The size and connectivity of cement-soil pores were significantly reduced,thereby improving cement-soil structural integrity. 展开更多
关键词 peat soil environment ultrafine cement(UFC) cement-soil strength test MICROSTRUCTURE
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Uranium Species in Peat and Rock Sediments Near Sludge Storage Facility
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作者 Olga Shvartseva Daria Mashkova 《Journal of Environmental & Earth Sciences》 2025年第2期187-197,共11页
The study aims to investigate uranium species in the sediments of the natural-technogenic system within a sludge storage facility in Russia.The relevance of this work is underscored by the need to assess the geochemic... The study aims to investigate uranium species in the sediments of the natural-technogenic system within a sludge storage facility in Russia.The relevance of this work is underscored by the need to assess the geochemical mobility of radionuclides,a critical factor for predicting their migration and environmental impact.The objective of the research was to determine the uranium species in both peat and sedimentary rock samples of the sludge storage facility and the adjacent area.Laboratory analyses included XRD,XRF analysis using synchrotron radiation,and scanning electron microscopy to study the composition and properties of minerals.The uranium species were further identified using a modified Tessier sequential extraction method.The results revealed that uranium predominantly occurs in a stable silicate-bound form(up to 80%)in sedimentary rocks,indicating minimal geochemical mobility.In contrast,in peat deposits,uranium is primarily associated with manganese and iron oxides(30–60%)as well as organic matter(5–40%),with the most mobile forms constituting less than 5%.The decrease in uranium concentration with distance from the facility was attributed to sorption onto organic matter and co-precipitation with mineral compounds,manganese and iron oxides,which serve as effective natural sorbents.The findings highlight the critical role of organic matter and metal oxides in limiting uranium migration,thus identifying them as key components in the formation of natural barriers for radionuclides.These results are crucial for assessing environmental risks associated with radioactive waste management and for developing strategies to minimize the ecological impact of sludge storages. 展开更多
关键词 URANIUM Sludge Storage Mineral Composition Uranium Speciation in Sediments Sorption by peat XRD SEM Safe Management
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Microstructure change rule during the consolidation process of peat soil from Yunnan province
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作者 Ruiling Feng Ou Wang +2 位作者 Zhenhao Zhang Jing Huang Yanping Wang 《High-Speed Railway》 2025年第4期293-304,共12页
Peat soil is a loose,moisture-rich organic matter accumulation formed by the deposition of plants in swamps and lakes after their death.It is characterized by high moisture content,large void ratio,high compressibilit... Peat soil is a loose,moisture-rich organic matter accumulation formed by the deposition of plants in swamps and lakes after their death.It is characterized by high moisture content,large void ratio,high compressibility,and strong rheological properties.These characteristics result in a complex consolidation process.A systematic understanding of the consolidation mechanism of peat soil is essential for elucidating its consolidation behavior.Previous studies have failed to provide consistent information on the microscopic morphology of peat soil.Moreover,quantitative studies on pore structure changes during peat soil consolidation remain lacking.To resolve these research gaps,the microscopic morphology and pore types of peat,highly organic peaty soil,and medium organic peaty soil from certain regions of Yunnan province,China,were observed and analyzed using scanning electron microscopy.Additionally,quantitative research on pore structure changes during peat soil consolidation was conducted.The results show that the humic acid in peat soil of Yunnan province has no pores,and there is no pore between humic acid and clay minerals.There are three typical pore structures,and the three typical pores were quantitatively analyzed.During consolidation,the consolidation deformation of peat soil is primarily caused by the internal pore compression of plant residues and pores between plant residues.At the same time,the revelation of the differentiated influence mechanism of load levels on the compression of inter/intra-plant residue pores.The decrease in the proportion of pores between plant residues first increased and then decreased with an increase in load,reaching a peak between 100-200 kPa.The decrease in pores inside the plant residues increased with an increasing load.Additionally,pore compression between the plant residues under different load levels primarily caused the compression deformation of Dali peat during the primary consolidation stage.By contrast,the pore compression inside the plant residues primarily caused the compression deformation during the secondary consolidation stage. 展开更多
关键词 peat soil CONSOLIDATION Pores between plant residues Pores inside plant residues Quantitative studies
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微生物蛋白激发子PeaT1的获得及诱导水稻抗旱性的初步研究 被引量:14
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作者 刘权 李广悦 +2 位作者 曾洪梅 杨秀芬 邱德文 《中国农业科技导报》 CAS CSCD 2009年第3期51-55,共5页
PeaT1是来源于极细链格胞菌(Alternaria tenuissima)的一种蛋白激发子,具有促进植物生长和提高抗逆性的功能。为了进一步研究该激发子的功能,构建了表达该蛋白质的原核表达载体pET28a-peaT1,诱导表达获得大量目的蛋白质。利用AKTA蛋白... PeaT1是来源于极细链格胞菌(Alternaria tenuissima)的一种蛋白激发子,具有促进植物生长和提高抗逆性的功能。为了进一步研究该激发子的功能,构建了表达该蛋白质的原核表达载体pET28a-peaT1,诱导表达获得大量目的蛋白质。利用AKTA蛋白质纯化系统,通过亲和层析、离子交换层析和分子筛等纯化技术,获得高纯度的PeaT1蛋白。用不同浓度的纯化蛋白处理水稻,进行抗旱性检测,结果表明PeaT1可明显提高水稻的抗旱性。 展开更多
关键词 peat1 蛋白激发子 原核表达 纯化 抗旱性
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极细链格孢菌peaT1基因在毕赤酵母中的表达与功能分析 被引量:7
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作者 刘延锋 曾洪梅 +3 位作者 玉山江 杨秀芬 毛建军 邱德文 《生物工程学报》 CAS CSCD 北大核心 2009年第3期413-417,共5页
建立了在毕赤酵母(Pichiapastoris)中分泌表达PeaT1蛋白的技术。将来源于极细链格孢菌(Alternaria tenuissima)的基因peaT1亚克隆至酵母分泌型表达载体pPIC9K,构建了重组表达载体pPIC9K-peaT1,分别用SalI或BglII酶切线性化后电击转入毕... 建立了在毕赤酵母(Pichiapastoris)中分泌表达PeaT1蛋白的技术。将来源于极细链格孢菌(Alternaria tenuissima)的基因peaT1亚克隆至酵母分泌型表达载体pPIC9K,构建了重组表达载体pPIC9K-peaT1,分别用SalI或BglII酶切线性化后电击转入毕赤酵母GS115菌株中,经MD平板筛选、PCR鉴定获得整合有外源基因的重组菌株。在α-Factor及AOX1基因启动子和终止信号的调控下,PeaT1在酵母中大量表达并分泌到胞外,SDS-PAGE检测表明表达蛋白的表观分子量约为35kD。表达蛋白上清稀释液能诱导烟草产生对TMV的抗性,其枯斑数抑制率可达到30.37%。每升表达上清液经超滤浓缩和离子交换层析可纯化目的蛋白16.13mg,该纯化蛋白能显著地促进小麦幼苗的生长。 展开更多
关键词 peat1 极细链格孢菌 毕赤酵母
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Effects of peat and weathered coal on the growth of Pinus sylvestrisvar. mongolica seedlings on aeolian sandy soil 被引量:3
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作者 陈伏生 陈广生 +1 位作者 曾德慧 梁超 《Journal of Forestry Research》 SCIE CAS CSCD 2002年第4期251-254,共4页
The experiment was conducted at the Ganqika Sandy Land Ecological Station in Ke抏rqinzuoyihouqi County, Inner Mongolia, in a growing season from April 28 to October 28, 2001. Peat and weathered coal were added to the ... The experiment was conducted at the Ganqika Sandy Land Ecological Station in Ke抏rqinzuoyihouqi County, Inner Mongolia, in a growing season from April 28 to October 28, 2001. Peat and weathered coal were added to the aeolian sandy soil in different ratios. Two-year-old Pinus sylvestris var. mongolica seedlings and plastic pots were used in the experiment. The experimental results indicated that: 1) the peat and weathered coal could significantly improve the physical and chemical prop-erties of aeolian sandy soil, and thus promoted the growth of seedlings; 2) the effect of peat on seedling growth, including height, base diameter, root length and biomass, presented an order of 8%>10%>5%>2%>0 in terms of peat contents, and the effect of weathered coal on seedling growth presented an order of 5%>8%>10%>2%>0 in terms of weathered coal contents for height and basal diameter, 5%>8%>2% >10%>0 for root length, and 5%>2%>8% >10%>0 for biomass; 3) the effects of peat were generally greater than that of weathered coal. Meanwhile, 8% peat was the best treatment to promote the growth of P. sylvestris var. mongolica seedlings. 展开更多
关键词 peat Weathered coal Aeolian sandy soil Pinus sylvestris var. mongolica SEEDLINGS GROWTH
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蛋白激发子PeaT1的高密度发酵及生理功能检测 被引量:3
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作者 刘权 李广悦 +2 位作者 曾洪梅 杨秀芬 邱德文 《生物技术通报》 CAS CSCD 北大核心 2009年第8期162-165,共4页
PeaT1是从极细链格孢菌(Alternaria tenuissima)中提取出的一种植物蛋白激发子,具有促进植物生长,增强作物抗逆性的功能。由于该蛋白具有无残留、无毒副作用的优点,因此具有发展为生物农药的应用前景。在100L发酵罐中利用分批补料技术对... PeaT1是从极细链格孢菌(Alternaria tenuissima)中提取出的一种植物蛋白激发子,具有促进植物生长,增强作物抗逆性的功能。由于该蛋白具有无残留、无毒副作用的优点,因此具有发展为生物农药的应用前景。在100L发酵罐中利用分批补料技术对PeaT1工程菌进行高密度发酵,通过AKTA蛋白纯化仪对发酵产物进行了亲和纯化,并检测了纯化所得蛋白对水稻在15℃低温下生长的影响。发酵最终菌体密度达80g/L,每升菌液纯化得到蛋白90mg,纯化所得蛋白能够促进水稻低温下的生长,具有激发子活性。 展开更多
关键词 蛋白激发子 peat1 高密度发酵 低温
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极细链格孢菌蛋白激发子Peat1在酵母双杂交系统中转录激活活性检测 被引量:4
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作者 刘延锋 邱德文 +1 位作者 曾洪梅 杨秀芬 《安徽农业科学》 CAS 北大核心 2008年第20期8500-8501,共2页
以PCR法从pGEX-6p-1-peaT1中扩增出peaT1基因片段,电泳回收后将peaT1基因定向克隆到plexA载体中。将诱饵载体plexA-peaT1经酶切和测序鉴定后,用PEG/LiAC法转化酵母EGY48[p8op-lacZ],并进行诱饵载体转录激活活性检测。结果表明,重组质粒... 以PCR法从pGEX-6p-1-peaT1中扩增出peaT1基因片段,电泳回收后将peaT1基因定向克隆到plexA载体中。将诱饵载体plexA-peaT1经酶切和测序鉴定后,用PEG/LiAC法转化酵母EGY48[p8op-lacZ],并进行诱饵载体转录激活活性检测。结果表明,重组质粒经EcoR I和XhoⅠ双酶切后,琼脂糖凝胶电泳检测可见2条与预期相符的条带,表明诱饵载体构建成功。β-半乳糖苷酶活性分析表明,诱饵载体plexA-peat1无转录激活活性,对酵母菌株也无毒害作用。该诱饵载体可用于酵母双杂交系统中,为下一步筛选cDNA文库奠定了基础。 展开更多
关键词 peat1 酵母双杂交 转录激活活性
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Monosaccharide Recovery from Peat and Peatified Wood by Ultrasonication Pretreatment and Hydrothermal Treatment 被引量:1
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作者 Moriyasu Nonaka Tsuyoshi Hirajima Satoshi Kumagai 《International Journal of Clean Coal and Energy》 2017年第2期31-39,共9页
Peat and peatified wood are significant carbohydrate resources in tropical rainforests. The carbohydrates of cellulose and hemicellulose are important sources of monosaccharides for both vital activities and industria... Peat and peatified wood are significant carbohydrate resources in tropical rainforests. The carbohydrates of cellulose and hemicellulose are important sources of monosaccharides for both vital activities and industrial applications, such as furan production of furfural and hydroxymethyl furfural. Hydrothermal treatment at 200°C and ultrasonic irradiation pretreatment were used to recover monosaccharides from the abovementioned resources. The monosaccharide recovery from peat was shown to be higher than that from peatified wood. The conversion to organic acids is considered to proceed rapidly because acids are always detected with monosaccharides. This conversion is outstripped by the organic acid-to-gas reaction for treatment times longer than 20 min. The monosaccharide recovery from peatified wood was improved by the ultrasonication pretreatment. It is considered that ultrasonic irradiation broke down lignin blockages, enabling water molecules to access the carbohydrates more easily in the subsequent hydrothermal treatment. 展开更多
关键词 peat peatified WOOD HYDROTHERMAL Treatment Ultrasonication MONOSACCHARIDE
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Study on Peat-Forming Mechanism of Tropic and Sub-TropicTidal Flats
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作者 桑树勋 刘焕杰 施健 《International Journal of Mining Science and Technology》 SCIE EI 1997年第2期51-55,共5页
Tropic and sub-tropic tidal flats (generalised) are a kind of speific peat-forming environments. Taking the Mangrove tidal flats and mangrove pets in Hainan island as a example, the authors deeply study and discuss th... Tropic and sub-tropic tidal flats (generalised) are a kind of speific peat-forming environments. Taking the Mangrove tidal flats and mangrove pets in Hainan island as a example, the authors deeply study and discuss the peat-forming mechanism of tropic and sub-tropic tidal flats. The conclusions are as follows: intrusion accretiou of plant organic matter is more important ; the organic matter has accumulated witk high productivity, high return rate and high decompeition rate ;anaerobic bacterin play a more important role during early peatification ; sea level change is the fundameutal factor controlling the formation and evolution of tidal flat peat-forming environments; lagoon peat-flat and delta peat-flat are main types of tidal flat peat-forming euvironments ;tidal flat peat-forming environments are remarkably characterized by low-level and rabe properties. 展开更多
关键词 MANGROVE TIDAL flat MANGROVE peat peat-forming mechanism TROPIC and subtropic ZONES
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激活蛋白PeaT1在烟草细胞膜上的结合位点及其特性 被引量:6
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作者 郑建华 杨秀芬 +2 位作者 石庆华 曾洪梅 邱德文 《植物病理学报》 CAS CSCD 北大核心 2010年第4期364-372,共9页
激活蛋白PeaT1是从极细链格孢菌(Alternaria tenuissima)中分离的,能诱导植物产生系统获得抗性的蛋白激发子。为了阐明PeaT1诱导植物提高抗性的分子机制,本文采用RT-PCR技术研究了PeaT1诱导烟草悬浮细胞后不同时间PR-1a基因的转录活性,... 激活蛋白PeaT1是从极细链格孢菌(Alternaria tenuissima)中分离的,能诱导植物产生系统获得抗性的蛋白激发子。为了阐明PeaT1诱导植物提高抗性的分子机制,本文采用RT-PCR技术研究了PeaT1诱导烟草悬浮细胞后不同时间PR-1a基因的转录活性,在烟草悬浮细胞中加入20μg/mL的PeaT18h后,PR-1a基因转录活性达到最高;相同浓度的PeaT1处理烟草植株,烟草叶片中酸性PR蛋白表达量升高。激光共聚焦显微镜观察到FITC荧光素标记的PeaT1可与烟草悬浮细胞表面结合;免疫荧光法进一步证明PeaT1可与烟草细胞质膜结合;使用共价交联剂BS3证明125I-PeaT1可与烟草细胞质膜上的2个分子结合,而与加热或蛋白酶处理的质膜不能发生交联,说明与PeaT1结合的分子具有蛋白特性,分子量分别为20kDa和30kDa。上述实验结果证明在烟草细胞质膜上存在着激发子PeaT1受体样的结合位点。 展开更多
关键词 peat1 PR蛋白 结合位点 质膜
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Effects of Lateral Variation in Vegetation and Basin ‘Dome' Shape on Tropical Lowland Peat Stabilisation in the Kota Samarahan-Asajaya Area, West Sarawak, Malaysia
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作者 Mohamad Tarmizi Mohamad ZULKIFLEY Tham Fatt NG +2 位作者 John Kuna RAJ Roslan HASHIM Muhammad Aqeel ASHRAF 《Acta Geologica Sinica(English Edition)》 SCIE CAS CSCD 2014年第3期894-914,共21页
Field surveys indicate lateral variation in peat humification levels (von Post) in dominantly occurring fibric,fibric to hemic,sapric and hemie to sapric peats across a gradient from the margin towards the centre of... Field surveys indicate lateral variation in peat humification levels (von Post) in dominantly occurring fibric,fibric to hemic,sapric and hemie to sapric peats across a gradient from the margin towards the centre of tropical lowland peat domes.Cement-peat stabilisation can be enhanced by adding mineral soil fillers (silt,clays and fine sands) obtained from Quaternary floodplain deposits and residual soil (weathered schist).The unconfined compressive strength (UCS) of the stabilised cement-mineral soil fifler-peat mix increases with the increased addition of selected mineral soil filler.Lateral variation in the stabilised peat strength (UCS) in the top 0 to 0.5 m layer was found from the margin towards the centre of the tropical lowland peat dome.The variations in the UCS of stabilised tropical lowland peats along a gradient from the periphery towards the centre of the peat dome are most likely caused by a combination of factors due to variations in the mineral soil or ash content of the peat and horizontal zonation or lateral variation in the dominant species of the plant assemblages (due to successive vegetation zonation of the peat swamp forest from the periphery towards the centre of the tropical lowland peat dome). 展开更多
关键词 tropical lowland peat dome/basin mineral soil filler cement-filler-peat stabilisation topogenic unconfined compressive strength lateral vegetation succession.
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Peat properties of a tropical forest reserve adjacent to a fire-break canal
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作者 Dayang Nur Sakinah Musa Mohd Zahirasri Mohd Tohir +3 位作者 Xinyan Huang Luqman Chuah Abdullah Mohamad Syazaruddin Md Said Muhammad Firdaus Sulaiman 《Journal of Forestry Research》 SCIE EI CAS CSCD 2024年第1期155-167,共13页
Tropical peat comprises decomposed dead plant material and acts like a sponge to absorb water,making it fully saturated.However,drought periods dry it readily and increases its vulnerability to fire.Peat fires emit gr... Tropical peat comprises decomposed dead plant material and acts like a sponge to absorb water,making it fully saturated.However,drought periods dry it readily and increases its vulnerability to fire.Peat fires emit greenhouse gases and particles contributing to haze,and prevention by constructing fire-break canals to reduce fire spread into forest reserves is crucial.This paper aims to determine peat physical and chemical properties near a fire-break canal at different fire frequency areas.Peat sampling was conducted at two forest reserves in Malaysia which represent low fire frequency and high fire frequency areas.The results show that peat properties were not affected by the construction of a fire-break canal,however lignin and cellulose content increased significantly from the distance of the canal in both areas.The study concluded that fire frequency did not significantly influence peat properties except for porosity.The higher fibre content in the high frequency area did not influence moisture content nor the ability to regain moisture.Thus,fire frequency might contribute differently to changes in physical and chemical properties,hence management efforts to construct fire-break canals and restoration efforts should protect peatlands from further degradation.These findings will benefit future management and planning for forest reserves. 展开更多
关键词 peat fire peat properties Fire-break canals Forest reserves
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DEPOSITS OF THE MANGROVE TIDAL FLATSAND MANGROVE PEATS IN HAINAN ISLAND
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作者 刘焕杰 桑树勋 施建 《Journal of China University of Mining and Technology》 1995年第2期11-22,共12页
In this paper, the environment characteristics, micro-environment division and evolution of the mangrove tidal flats, peats and their genetic markers are discussed. It proves that the mangrove tidal flat is a kind of ... In this paper, the environment characteristics, micro-environment division and evolution of the mangrove tidal flats, peats and their genetic markers are discussed. It proves that the mangrove tidal flat is a kind of tidal flats and the peat flats are developed in a specific evolution stage of mangrove tidal flats; the mangrove peats are the products of a specific evolution stage of the mangrove tidal flats. 展开更多
关键词 tidal flat mangrove tidal flat peat flat mangrove peat Hainan Island
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蛋白激发子基因peaT1植物表达载体的构建及其转化棉花的研究 被引量:1
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作者 唐宏琨 曾洪梅 +2 位作者 杨秀芬 袁京京 邱德文 《植物保护》 CAS CSCD 北大核心 2011年第3期43-47,共5页
[目的]构建蛋白激发子基因peaT1的植物表达载体并转化棉花品种‘CCRI24’。[方法]设计含有PstⅠ和XhoⅠ酶切位点的引物,以质粒pET28a-peaT1为模板扩增得到peaT1序列,将其通过中间载体pG4AS-cup克隆到植物表达载体pCAMBIA2300上,通过农... [目的]构建蛋白激发子基因peaT1的植物表达载体并转化棉花品种‘CCRI24’。[方法]设计含有PstⅠ和XhoⅠ酶切位点的引物,以质粒pET28a-peaT1为模板扩增得到peaT1序列,将其通过中间载体pG4AS-cup克隆到植物表达载体pCAMBIA2300上,通过农杆菌介导法转化棉花品种‘CCRI24’,诱导并筛选抗性愈伤和胚性愈伤,通过PCRs、outhern杂交和RT-PCR检测筛选的棉株。[结果]构建了含有增强子和多联终止子的植物表达载体pCAMBIA2300-peaT1,获得了大量的胚状体和4棵再生苗并嫁接成活,验证了蛋白激发子基因peaT1已经整合到再生苗2和4基因组当中。[结论]本研究为进一步开展蛋白激发子基因peaT1转化棉花的研究提供了基础材料。 展开更多
关键词 蛋白激发子 peat1 农杆菌 转化 棉花
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细极链格孢菌peaT2基因在毕赤酵母中的表达及蛋白功能确定 被引量:1
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作者 刘文平 曾洪梅 +2 位作者 刘延锋 袁京京 邱德文 《微生物学报》 CAS CSCD 北大核心 2007年第4期593-597,共5页
从细极链格孢菌表达文库获得阳性克隆子,序列分析表明,克隆的DNA片段中含有完整的开放阅读框架,将该基因命名为peaT2(GenBank登录号为EF212880)。用PCR法扩增peaT2基因的编码序列并亚克隆到毕赤酵母表达系统的表达载体pPIC9K上,得到重... 从细极链格孢菌表达文库获得阳性克隆子,序列分析表明,克隆的DNA片段中含有完整的开放阅读框架,将该基因命名为peaT2(GenBank登录号为EF212880)。用PCR法扩增peaT2基因的编码序列并亚克隆到毕赤酵母表达系统的表达载体pPIC9K上,得到重组质粒pPIC9K/peaT2。重组质粒经SacⅠ线性化后用电穿孔法导入到毕赤酵母(Pichia pastoris)GS115中,采用MD、G418-YPD平板和PCR法筛选Mut+表型,获得了分泌表达的重组毕赤酵母。随机挑取一菌株作为表达菌,用甲醇诱导PeaT2蛋白表达。SDS-PAGE及Western blot检测结果均表明PeaT2在毕赤酵母中成功地分泌表达。用peaT2基因的表达蛋白处理小麦种子,生物测定表明,表达蛋白能明显促进小麦的生长,具有蛋白激发子作用。 展开更多
关键词 peat2 蛋白激发子 细极链格孢菌 毕赤酵母表达系统
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蛋白激发子基因peaT1转基因棉花的获得及初步鉴定 被引量:1
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作者 唐宏琨 曾洪梅 +4 位作者 杨秀芬 李国媛 盛德鹏 袁京京 邱德文 《生物技术通报》 CAS CSCD 北大核心 2012年第1期60-64,共5页
将来源于极细链格孢菌的蛋白激发子基因peaT1克隆到植物表达载体pCAMBIA2300上,成功构建了含有增强子和多联终止子的植物表达载体pCAMBIA2300-G4AS-peaT1。通过花粉管通道法转化棉花,经卡那霉素抗性筛选、PCR和RT-PCR检测获得一株转基... 将来源于极细链格孢菌的蛋白激发子基因peaT1克隆到植物表达载体pCAMBIA2300上,成功构建了含有增强子和多联终止子的植物表达载体pCAMBIA2300-G4AS-peaT1。通过花粉管通道法转化棉花,经卡那霉素抗性筛选、PCR和RT-PCR检测获得一株转基因棉花植株,其植株茎粗和成铃数均高于非转基因对照株。 展开更多
关键词 蛋白激发子 peat1 花粉管通道 棉花
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Dicyandiamide Sorption-Desorption Behavior on Soils and Peat Humus 被引量:15
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作者 ZHANGHai-Jun WUZhi-Jie ZHOUQi-Xing 《Pedosphere》 SCIE CAS CSCD 2004年第3期395-399,共5页
The sorption-desorption behavior of dicyandiamide (DCD) is an importantchemical process that affects DCD fate and mobility in soils. Therefore, this study quantified DCDsorption-desorption on a phaeozem (Mollisol), a ... The sorption-desorption behavior of dicyandiamide (DCD) is an importantchemical process that affects DCD fate and mobility in soils. Therefore, this study quantified DCDsorption-desorption on a phaeozem (Mollisol), a burozem (Alfisol), a soil with organicmatter-removed and peat humus using the batch-equilibration procedure, and identified soilproperties that influenced DCD sorption. The sorption on peat humus was higher than that on thephaeozem and the burozem, with much lower sorption observed on the soil with organic matter-removed,indicating that soil organic matter was the main carrier of DCD sorption. Due to its amphipathicproperty the DCD molecule sorption on the phaeozem and the burozem decreased as pH increased fromabout 2 to 5, but a further increase in pH led to a rise in DCD sorption. The DCD desorptionhysteretic effect for peat humus was greater than that for the phaeozem and the burozem using 0.01mol L^(-1) CaCl_2 as the background electrolyte, suggesting that the hydrophobic domains of organicmatter may play an important role in DCD sorption. 展开更多
关键词 DICYANDIAMIDE peat humus soil sorption-desorption behavior
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Nitrogen Removal Improvement by Adding Peat in Deep Soil of Subsurface Wastewater Infi ltration System 被引量:6
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作者 CHEN Pei-zhen CUI Jian-yu +4 位作者 HU Lin ZHENG Miao-zhuang CHENG Shan-ping HUANG Jie-wen MU Kang-guo 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2014年第5期1113-1120,共8页
In order to enhance the nitrogen removal, a subsurface wastewater infiltration system (SWIS) was improved by adding peat in deep soil as carbon source for denitrification process. The effects of addition of carbon s... In order to enhance the nitrogen removal, a subsurface wastewater infiltration system (SWIS) was improved by adding peat in deep soil as carbon source for denitrification process. The effects of addition of carbon source in the underpart of the SWIS on nitrogen removal at different influents (with the total nitrogen (TN) concentration 40 and 80 mg L^-1, respectively) were investigated by soil column simulating experiments. When the relatively light pollution influent with 40 mg L^-1 TN was used, the average concentrations of NO3-N and TN in effluents were (4.69±0.235), (6.18±0.079) mg L^-1, respectively, decreased by 32 and 30.8% than the control; the NO3--N concentration of all effluents was below the maximum contaminant level of 10 mg L^-1; as high as 92.67% of the TN removal efficiency was achieved. When relatively heavy pollution influent with 80 mg LITN was used, the average concentrations of NO3--N and TN in effluents were (10.2±0.265), (12.5±0.148) mg L^-1 respectively, decreased by 20 and 21.2% than the control; the NO3--N concentration of all effluents met the grade Ⅲ of the national quality standard for ground water of China (GB/T 14848-1993) with the values less than 20 mg L^-1; the TN removal efficiency of 94.1% was achieved. In summary, adding peat in the underpart of the SWIS significantly decreased TN and NO3- -N concentration in effluents and the nitrogen removal efficiency improved significantly. 展开更多
关键词 peat addition nitrogen removal efficiency DENITRIFICATION subsurface wastewater infiltration system
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激发子基因peaT1转化三生烟及其对TMV抗性的提高
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作者 唐宏琨 曾洪梅 +1 位作者 杨秀芬 邱德文 《生物技术通报》 CAS CSCD 北大核心 2010年第10期116-119,134,共5页
通过根癌农杆菌(Agrobactrium tumefaciens)介导转化法,将含有激发子基因peaT1的植物表达载体pCAM-BIA2300-G4AS-peaT1转化三生烟,获得了转基因烟草植株。用PCR检测确认了阳性转化株,用Southern杂交、RT-PCR和Western杂交进一步证实了pe... 通过根癌农杆菌(Agrobactrium tumefaciens)介导转化法,将含有激发子基因peaT1的植物表达载体pCAM-BIA2300-G4AS-peaT1转化三生烟,获得了转基因烟草植株。用PCR检测确认了阳性转化株,用Southern杂交、RT-PCR和Western杂交进一步证实了peaT1基因的整合、转录和表达。对T1代转基因阳性株进行TMV接种试验,结果显示,与非转基因对照相比,表达peaT1的烟草叶片枯斑数量减少,表明蛋白激发子基因peaT1的表达提高了转基因烟草对TMV的抗性。 展开更多
关键词 激发子 peat1基因 烟草 转化TMV
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