摘要
将来源于极细链格孢菌的蛋白激发子基因peaT1克隆到植物表达载体pCAMBIA2300上,成功构建了含有增强子和多联终止子的植物表达载体pCAMBIA2300-G4AS-peaT1。通过花粉管通道法转化棉花,经卡那霉素抗性筛选、PCR和RT-PCR检测获得一株转基因棉花植株,其植株茎粗和成铃数均高于非转基因对照株。
The protein elicitor-encoding gene peaT1 obtained from Alternaria tenuissima was inserted to pCAMBIA2300 to construct the plant expressive vector pCAMBIA2300-G4AS-peaT1 which contains enhancer and poly-terminator.Then,the resulting vector was transferred into cotton by pollen-tube pathway method.The positive transgenic line was screened with kanamycin resistance,PCR,and RT-PCR.The resultant plant got more cotton bolls and the stem diameter was higher compared with the control.
出处
《生物技术通报》
CAS
CSCD
北大核心
2012年第1期60-64,共5页
Biotechnology Bulletin
基金
转基因重大专项(2008ZX08010-004
2009EX08001-018B)
