【Objective】This study aimed to establish a quintuple PCR method for rapid and simultaneous detection of Ralstonia solanacearum,Fusarium spp.,Pectobacterium spp.,Enterobacter spp.,and Pythium spp.,which provided tech...【Objective】This study aimed to establish a quintuple PCR method for rapid and simultaneous detection of Ralstonia solanacearum,Fusarium spp.,Pectobacterium spp.,Enterobacter spp.,and Pythium spp.,which provided technical support for early diagnosis of various soil-borne diseases on ginger.【Method】For five types of soil-borne pathogens causing ginger bacterial wilt and rhizome rot,specific primer combinations were designed and screened,the optimal quintuple reaction system was established by exploring optimal primer concentrations,annealing temperature,and sensitivity,and was applied to detect field plant samples to verify its utility.【Result】Specific primers pairs Rs1F/Rs1R,En1F/En1R,and Py1F/Py1R were designed according to flic gene of Ralstonia solanacearum,rpoB gene of Enterobacter spp.,and 18S rDNA of Pythium spp.,and combined with reported Fusarium spp.specific primers Fu3/Fu4 and specific primers 23SPecF/23SPecR of Pectobacterium spp.,a quintuple PCR reaction system for ginger soil-borne pathogens has been established(25.00μL):above primer dosage was 1.20,0.20,0.60,1.60,and 0.15μL respectively;2×PCR Mix 12.50μL;DNA templates of different pathogens were 1.00μL each;added ddH_(2)O to 25.00μL.Annealing temperature was optimized to 55.4℃.The specific fragments with sizes of 516,370,266,207,and 159 bp could be amplified simultaneously in the established quintuple PCR system,and the detection limit of this system for Ralstonia solanacearum,Enterobacter spp.and Pythium spp.reached 10^(-1)pg/μL,for Fusarium spp.and Pectobacterium spp.was 1 pg/μL,and for detecting five pathogens simultaneously was 10^(3)pg/μL.The multiplex PCR system established in this study could successfully detect the diseased plant samples from the field.【Conclusion】The quintuple PCR system established is able to rapid ly and accurately detect Ralstonia solanacearum,Fusarium spp.,Pectobacterium spp.,Enterobacter spp.,and Pythium spp.,which provides a useful tool for timely diagnosis and epidemic monitoring of various soil-borne diseases of ginger.展开更多
Asymmetric stators,featuring nonuniform pitches,have demonstrated effectiveness in mitigating the forced response of the adjacent compressor rotor blades.However,the lack of comprehensive understanding of their vibrat...Asymmetric stators,featuring nonuniform pitches,have demonstrated effectiveness in mitigating the forced response of the adjacent compressor rotor blades.However,the lack of comprehensive understanding of their vibration reduction mechanisms hinders the development of optimal designs.Typically,the evaluation of rotor blades forced response using asymmetric stators requires fluid–structure interaction methods and full-annulus computational domains;however,these methods are time-consuming and resource-intensive,making them unsuitable for rapid engineering applications.To address these issues,the present study first develops a Fourier-based prediction method for the excitation spectrum and blade forced response that considers the impacts of multiple excitation components.To verify the accuracy of the prediction method,two typical asymmetric stator configurations are selected,and the forced response analyses with single-passage computational domains are conducted on their downstream rotor blades based on the rapid time inclination method.The results are then compared with those obtained using the dual time stepping method with whole-annulus computational domains.The results indicate that the proposed Fourier-based method can accurately predict the impacts of asymmetric stators on the forced response of the rotor blades.Moreover,the rapid evaluation approach based on the time inclination method provides comparable accuracy to the dual time stepping method,but with greater computational efficiency and reduced memory consumption.展开更多
[Objectives]To investigate the effects of three cultivation methods-level bed,high ridge,and ridge cultivation-on the yield and quality of Radix scutellariae,providing a basis for establishing its Good Agricultural Pr...[Objectives]To investigate the effects of three cultivation methods-level bed,high ridge,and ridge cultivation-on the yield and quality of Radix scutellariae,providing a basis for establishing its Good Agricultural Practice(GAP)technical system.[Methods]Utilizing two-year-old R.scutellariae plants from the cultivation base in Kushan Township,Ju County as the experimental material,yield-related indicators including root length,root diameter,branch number,and fresh weight were measured at harvest time.The baicalin content in the roots was determined using High-Performance Liquid Chromatography(HPLC),and the differences in effects among the cultivation methods were analyzed.[Results]R.scutellariae cultivated using the ridge method exhibited significantly superior root length compared to both level bed and high ridge cultivation.Its branch number was significantly higher than that under level bed cultivation.The baicalin content reached 13.17%,surpassing that achieved with high ridge(12.73%)and level bed(11.87%)cultivation.Based on a comprehensive evaluation of agronomic traits and active constituent content,R.scutellariae cultivated using the ridge method demonstrated significant superiority in both yield and quality over those grown under high ridge or level bed conditions.[Conclusions]Ridge cultivation effectively promotes root growth in R.scutellariae,enhances medicinal material yield and baicalin content.This method is recommended for widespread application in artificial cultivation and provides a scientific foundation for establishing R.scutellariae GAP standards.展开更多
As a novel class of purely organic fluores-cent materials,multiple resonance thermal-ly activated delayed fluorescence(MR-TADF)compounds hold significant promise for next-generation display technologies.The efficiency...As a novel class of purely organic fluores-cent materials,multiple resonance thermal-ly activated delayed fluorescence(MR-TADF)compounds hold significant promise for next-generation display technologies.The efficiency of exciton utilization and the overall performance of organic light-emit-ting devices are closely linked to the singlet-triplet energy gap(ΔE_(ST))of MR-TADF emitters.Identifying an economic and accu-rate theoretical approach to predictΔE_(ST)would be beneficial for high-throughput screening and facilitate the inverse design of MR-TADF molecules.In this study,we evaluated the S_(1)state energy(E(S_(1))),T_(1)state ener-gy(E(T_(1))),andΔE_(ST)using three different physical interpretations:adiabatic excitation ener-gy,vertical absorption energy,and vertical emission energy.We employed the time-depen-dent density functional theory(TDDFT)and delta self-consistent field(ΔSCF)methods to calculate E(S_(1)),E(T_(1)),andΔE_(ST)for 20 MR-TADF molecules reported in the literature.We compared these calculated values with experimental data obtained from fluorescence spec-troscopy at room-temperature(or 77 K)and phosphorescence spectroscopy conducted at 77 K.Our findings indicate that the vertical absorption energy at the S0 state minimum,deter-mined by theΔSCF method,accurately predicts the S_(1)state energy.Similarly,the vertical absorption energy at the S0 state minimum,calculated using the TDDFT method,effectively predicts the T_(1)state energy.TheΔE_(ST)derived from the difference between these two excita-tion energies exhibited the smallest mean absolute error of only 0.039 eV compared to the ex-perimental values.This combination represents the most accurate and cost-effective method reported to date for predicting theΔE_(ST)of MR-TADF molecules,and can be integrated into AI-driven inverse design workflows for new emitters.展开更多
Objectives This review aimed to systematically synthesize the available research on the disclosure of diagnosis and related issues in childhood cancer from the perspectives of healthcare professionals,with the goal of...Objectives This review aimed to systematically synthesize the available research on the disclosure of diagnosis and related issues in childhood cancer from the perspectives of healthcare professionals,with the goal of informing the optimization of disclosure processes and meeting the communication needs of affected families.Methods In accordance with the Joanna Briggs Institute(JBI)methodology for mixed methods systematic reviews,the convergent segregated approach was used in this review.Articles were retrieved from 11 databases,including PubMed,Web of Science,CINAHL,CENTRAL,Embase,Ovid/Medline,PsycINFO,PsycArticles,Scopus,ERIC,and China National Knowledge Infrastructure(CNKI).The quality of the selected articles was assessed using the Mixed Method Appraisal Tool(MMAT).The review protocol was registered on PROSPERO(CRD42024542746).Results A total of 21 studies from 10 countries were included.Their methodological quality was generally medium to high,with MMAT scores ranging from 60%to 100%.The synthesis yielded three core themes:1)the spectrum of professional and societal attitudes toward disclosure;2)the dynamic practices of navigating disclosure amid uncertainty,including timing and environment,stakeholders,and content of disclosure;and 3)factors influencing disclosure,including children’s,parental,healthcare professionals’,and socio-cultural factors.Conclusions This review synthesized the perspectives and experiences of healthcare professionals regarding disclosure in childhood cancer,highlighting the complexity and multidimensional nature of this process in clinical practice.Future research should further investigate the experiences and needs of children and their parents,explore cultural variations in disclosure practices,develop context-appropriate assessment tools,and construct multidimensional intervention strategies to enhance the humanistic care and professional effectiveness of the disclosure process.展开更多
The Good Wife is an American TV series that focuses on women’s independence,politics,and law.The drama has been remade in China,Japan,and South Korea.This research aims to use Nida’s Functional Equivalence Theory to...The Good Wife is an American TV series that focuses on women’s independence,politics,and law.The drama has been remade in China,Japan,and South Korea.This research aims to use Nida’s Functional Equivalence Theory to analyze the methods of its English-to-Chinese subtitle translation by considering social,cultural,and historic backgrounds between China and America.After data collection and case analysis,the study found that:(1)Five major translation methods are adopted in the subtitle translation of The Good Wife.They are free translation,variation,literal translation,addition,and omission.Among them,free translation is the most frequently used,while omission is used least.(2)The subtitle translation of films and TV series is limited by time and space restrictions,social-cultural differences,and other factors.When translating,translators should try to use humorous words,euphemism,intonation,and other ways,and combine different methods such as literal translation,free translation,variation,addition,omission,and other methods to seek equivalence both in the meaning and function of subtitles under the guidance of Functional Equivalence Theory.展开更多
This paper studies high order compact finite volume methods on non-uniform meshes for one-dimensional elliptic and parabolic differential equations with the Robin boundary conditions.An explicit scheme and an implicit...This paper studies high order compact finite volume methods on non-uniform meshes for one-dimensional elliptic and parabolic differential equations with the Robin boundary conditions.An explicit scheme and an implicit scheme are obtained by discretizing the equivalent integral form of the equation.For the explicit scheme with nodal values,the algebraic system can be solved by the Thomas method.For the implicit scheme with both nodal values and their derivatives,the system can be implemented by a prediction-correction procedure,where in the correction stage,an implicit formula for recovering the nodal derivatives is introduced.Taking two point boundary value problem as an example,we prove that both the explicit and implicit schemes are convergent with fourth order accuracy with respect to some standard discrete norms using the energy method.Two numerical examples demonstrate the correctness and effectiveness of the schemes,as well as the indispensability of using non-uniform meshes.展开更多
Objective: To analyze the advantages of diversified teaching methods in the clinical instruction of operating room nursing interns. Methods: Twenty-one nursing interns who underwent internships in the operating room f...Objective: To analyze the advantages of diversified teaching methods in the clinical instruction of operating room nursing interns. Methods: Twenty-one nursing interns who underwent internships in the operating room from March 2023 to March 2024 were selected as the control group and received conventional teaching methods. Another twenty-one nursing interns who underwent internships in the operating room from April 2024 to April 2025 were selected as the experimental group and received diversified teaching methods. The teaching effects of the two groups were compared. Results: The experimental group scored higher than the control group in assessments, teaching satisfaction, and teaching quality evaluations, as well as in post-instruction professional identity scores (p < 0.05). Conclusion: Diversified teaching methods can enhance the professional competence of operating room nursing interns, cultivate their professional identity, and yield high teaching satisfaction, thereby improving teaching quality.展开更多
This paper presents an overview of the pathogens, symptoms of damage, patterns of occurrence, and chemical control methods associated with four major pear tree diseases: pear scab, pear ring rot, pear anthracnose, and...This paper presents an overview of the pathogens, symptoms of damage, patterns of occurrence, and chemical control methods associated with four major pear tree diseases: pear scab, pear ring rot, pear anthracnose, and pear speckle. The objective is to provide valuable references for the scientific and precise prevention and management of diseases in pear orchards, thereby contributing to the production of high-quality and high-yield pear fruits.展开更多
Geological prospecting and the identification of adverse geological features are essential in tunnel construction,providing critical information to ensure safety and guide engineering decisions.As tunnel projects exte...Geological prospecting and the identification of adverse geological features are essential in tunnel construction,providing critical information to ensure safety and guide engineering decisions.As tunnel projects extend into deeper and more mountainous terrains,engineers face increasingly complex geological conditions,including high water pressure,intense geo-stress,elevated geothermal gradients,and active fault zones.These conditions pose substantial risks such as high-pressure water inrush,largescale collapses,and tunnel boring machine(TBM)blockages.Addressing these challenges requires advanced detection technologies capable of long-distance,high-precision,and intelligent assessments of adverse geology.This paper presents a comprehensive review of recent advancements in tunnel geological ahead prospecting methods.It summarizes the fundamental principles,technical maturity,key challenges,development trends,and real-world applications of various detection techniques.Airborne and semi-airborne geophysical methods enable large-scale reconnaissance for initial surveys in complex terrain.Tunnel-and borehole-based approaches offer high-resolution detection during excavation,including seismic ahead prospecting(SAP),TBM rock-breaking source seismic methods,fulltime-domain tunnel induced polarization(TIP),borehole electrical resistivity,and ground penetrating radar(GPR).To address scenarios involving multiple,coexisting adverse geologies,intelligent inversion and geological identification methods have been developed based on multi-source data fusion and artificial intelligence(AI)techniques.Overall,these advances significantly improve detection range,resolution,and geological characterization capabilities.The methods demonstrate strong adaptability to complex environments and provide reliable subsurface information,supporting safer and more efficient tunnel construction.展开更多
[Objective] In this study, the quantitive detection of PCV2 （porcine circovirus type 2） in vitro was achieved. We aimed to establish two kinds of TaqMan real-time PCR methods based on PCV20RF1 and ORF2 respectively ...[Objective] In this study, the quantitive detection of PCV2 （porcine circovirus type 2） in vitro was achieved. We aimed to establish two kinds of TaqMan real-time PCR methods based on PCV20RF1 and ORF2 respectively and compare them. [Method] According to the relatively＇conserved sequences of PCV20RF1 and ORF2 registered in GenBank, two pairs of specific primers and TaqMan probes were designed and synthesized. Then the recombinant plasmids containing the whole sequences of PCV20RF1 and ORF2 were constructed to draw the standard curves through optimizing the reaction system and conditions. And thus two kinds of TaqMan real-time PCR detection methods based on the whole sequences of ORF1 and ORF2 respectively were constructed for PCV2. [Result] For the two established standard curves, the Ct values showed a good linear relationship with the loga- rithms of copy numbers of templates （F2〉0.99）. The amplification efficiency ranged from 90% to 110%. The amplifications all had a good repeatability with variation coefficients within groups all less than 5%. Moreover, the amplifications all had a good specificity. When the sequences of porcine parvovirus （PPV）, porcine circovirus type 1 （PCV1）, swine pseudorabies virus （PRV）, porcine reproductive and respiratory syndrome virus （PRRSV） were used as templates, the target sequence was not amplified. The amplifications also had a high sensitivity. The ORF1 detection method could reach 1.0x10T copies/;ul, and the ORF2 detection method could reach 1.0×10^2 copies/μl. The two established real-time PCR detection methods were used to detect the 80 clinical samples respectively. The results showed the magnitudes of 72 amplified samples were basically consistent between the 2 detection methods, while the magnitudes of the other 8 amplified samples were inconsistent. Then the 8 samples were detected with SYBR Green I real-time PCR method established based on the sequence of PCV2-1ike factor P1 by Wen et aL The PCV2-1ike factor P1 was amplified in all the 8 samples, indicating the 8 samples were all infected with PCV2-1ike factor P1. [Conclusion] The ORFl-based detection method has a higher accuracy, and it can be used for the rapid detection of PCV2.展开更多
基金National Natural Science Foundation of China(32270237)Guangxi Key Research and Development Plan Project(Guike AB21238002)Basic Scientific Research Project of Guangxi Academy of Agricultural Sciences(Guinongke 2024YP082)。
文摘【Objective】This study aimed to establish a quintuple PCR method for rapid and simultaneous detection of Ralstonia solanacearum,Fusarium spp.,Pectobacterium spp.,Enterobacter spp.,and Pythium spp.,which provided technical support for early diagnosis of various soil-borne diseases on ginger.【Method】For five types of soil-borne pathogens causing ginger bacterial wilt and rhizome rot,specific primer combinations were designed and screened,the optimal quintuple reaction system was established by exploring optimal primer concentrations,annealing temperature,and sensitivity,and was applied to detect field plant samples to verify its utility.【Result】Specific primers pairs Rs1F/Rs1R,En1F/En1R,and Py1F/Py1R were designed according to flic gene of Ralstonia solanacearum,rpoB gene of Enterobacter spp.,and 18S rDNA of Pythium spp.,and combined with reported Fusarium spp.specific primers Fu3/Fu4 and specific primers 23SPecF/23SPecR of Pectobacterium spp.,a quintuple PCR reaction system for ginger soil-borne pathogens has been established(25.00μL):above primer dosage was 1.20,0.20,0.60,1.60,and 0.15μL respectively;2×PCR Mix 12.50μL;DNA templates of different pathogens were 1.00μL each;added ddH_(2)O to 25.00μL.Annealing temperature was optimized to 55.4℃.The specific fragments with sizes of 516,370,266,207,and 159 bp could be amplified simultaneously in the established quintuple PCR system,and the detection limit of this system for Ralstonia solanacearum,Enterobacter spp.and Pythium spp.reached 10^(-1)pg/μL,for Fusarium spp.and Pectobacterium spp.was 1 pg/μL,and for detecting five pathogens simultaneously was 10^(3)pg/μL.The multiplex PCR system established in this study could successfully detect the diseased plant samples from the field.【Conclusion】The quintuple PCR system established is able to rapid ly and accurately detect Ralstonia solanacearum,Fusarium spp.,Pectobacterium spp.,Enterobacter spp.,and Pythium spp.,which provides a useful tool for timely diagnosis and epidemic monitoring of various soil-borne diseases of ginger.
基金supported by the Aeronautical Science Foundation of China(Nos.2023L039053002 and 2024M039053001)。
文摘Asymmetric stators,featuring nonuniform pitches,have demonstrated effectiveness in mitigating the forced response of the adjacent compressor rotor blades.However,the lack of comprehensive understanding of their vibration reduction mechanisms hinders the development of optimal designs.Typically,the evaluation of rotor blades forced response using asymmetric stators requires fluid–structure interaction methods and full-annulus computational domains;however,these methods are time-consuming and resource-intensive,making them unsuitable for rapid engineering applications.To address these issues,the present study first develops a Fourier-based prediction method for the excitation spectrum and blade forced response that considers the impacts of multiple excitation components.To verify the accuracy of the prediction method,two typical asymmetric stator configurations are selected,and the forced response analyses with single-passage computational domains are conducted on their downstream rotor blades based on the rapid time inclination method.The results are then compared with those obtained using the dual time stepping method with whole-annulus computational domains.The results indicate that the proposed Fourier-based method can accurately predict the impacts of asymmetric stators on the forced response of the rotor blades.Moreover,the rapid evaluation approach based on the time inclination method provides comparable accuracy to the dual time stepping method,but with greater computational efficiency and reduced memory consumption.
基金Supported by Shandong Provincial Traditional Chinese Medicine Science and Technology Development Plan Project 2019-2020(2019-1036)Special Subsidy for Public Health Services in Traditional Chinese Medicine"National Survey of Chinese Medicinal Material Resources"(CaiShe[2019]No.39),2019.
文摘[Objectives]To investigate the effects of three cultivation methods-level bed,high ridge,and ridge cultivation-on the yield and quality of Radix scutellariae,providing a basis for establishing its Good Agricultural Practice(GAP)technical system.[Methods]Utilizing two-year-old R.scutellariae plants from the cultivation base in Kushan Township,Ju County as the experimental material,yield-related indicators including root length,root diameter,branch number,and fresh weight were measured at harvest time.The baicalin content in the roots was determined using High-Performance Liquid Chromatography(HPLC),and the differences in effects among the cultivation methods were analyzed.[Results]R.scutellariae cultivated using the ridge method exhibited significantly superior root length compared to both level bed and high ridge cultivation.Its branch number was significantly higher than that under level bed cultivation.The baicalin content reached 13.17%,surpassing that achieved with high ridge(12.73%)and level bed(11.87%)cultivation.Based on a comprehensive evaluation of agronomic traits and active constituent content,R.scutellariae cultivated using the ridge method demonstrated significant superiority in both yield and quality over those grown under high ridge or level bed conditions.[Conclusions]Ridge cultivation effectively promotes root growth in R.scutellariae,enhances medicinal material yield and baicalin content.This method is recommended for widespread application in artificial cultivation and provides a scientific foundation for establishing R.scutellariae GAP standards.
基金support provided by the National Natural Science Foundation of China(No.22273043).
文摘As a novel class of purely organic fluores-cent materials,multiple resonance thermal-ly activated delayed fluorescence(MR-TADF)compounds hold significant promise for next-generation display technologies.The efficiency of exciton utilization and the overall performance of organic light-emit-ting devices are closely linked to the singlet-triplet energy gap(ΔE_(ST))of MR-TADF emitters.Identifying an economic and accu-rate theoretical approach to predictΔE_(ST)would be beneficial for high-throughput screening and facilitate the inverse design of MR-TADF molecules.In this study,we evaluated the S_(1)state energy(E(S_(1))),T_(1)state ener-gy(E(T_(1))),andΔE_(ST)using three different physical interpretations:adiabatic excitation ener-gy,vertical absorption energy,and vertical emission energy.We employed the time-depen-dent density functional theory(TDDFT)and delta self-consistent field(ΔSCF)methods to calculate E(S_(1)),E(T_(1)),andΔE_(ST)for 20 MR-TADF molecules reported in the literature.We compared these calculated values with experimental data obtained from fluorescence spec-troscopy at room-temperature(or 77 K)and phosphorescence spectroscopy conducted at 77 K.Our findings indicate that the vertical absorption energy at the S0 state minimum,deter-mined by theΔSCF method,accurately predicts the S_(1)state energy.Similarly,the vertical absorption energy at the S0 state minimum,calculated using the TDDFT method,effectively predicts the T_(1)state energy.TheΔE_(ST)derived from the difference between these two excita-tion energies exhibited the smallest mean absolute error of only 0.039 eV compared to the ex-perimental values.This combination represents the most accurate and cost-effective method reported to date for predicting theΔE_(ST)of MR-TADF molecules,and can be integrated into AI-driven inverse design workflows for new emitters.
基金supported by the Fuxing Nursing Research Foundation of Fudan University[FNF202352].
文摘Objectives This review aimed to systematically synthesize the available research on the disclosure of diagnosis and related issues in childhood cancer from the perspectives of healthcare professionals,with the goal of informing the optimization of disclosure processes and meeting the communication needs of affected families.Methods In accordance with the Joanna Briggs Institute(JBI)methodology for mixed methods systematic reviews,the convergent segregated approach was used in this review.Articles were retrieved from 11 databases,including PubMed,Web of Science,CINAHL,CENTRAL,Embase,Ovid/Medline,PsycINFO,PsycArticles,Scopus,ERIC,and China National Knowledge Infrastructure(CNKI).The quality of the selected articles was assessed using the Mixed Method Appraisal Tool(MMAT).The review protocol was registered on PROSPERO(CRD42024542746).Results A total of 21 studies from 10 countries were included.Their methodological quality was generally medium to high,with MMAT scores ranging from 60%to 100%.The synthesis yielded three core themes:1)the spectrum of professional and societal attitudes toward disclosure;2)the dynamic practices of navigating disclosure amid uncertainty,including timing and environment,stakeholders,and content of disclosure;and 3)factors influencing disclosure,including children’s,parental,healthcare professionals’,and socio-cultural factors.Conclusions This review synthesized the perspectives and experiences of healthcare professionals regarding disclosure in childhood cancer,highlighting the complexity and multidimensional nature of this process in clinical practice.Future research should further investigate the experiences and needs of children and their parents,explore cultural variations in disclosure practices,develop context-appropriate assessment tools,and construct multidimensional intervention strategies to enhance the humanistic care and professional effectiveness of the disclosure process.
文摘The Good Wife is an American TV series that focuses on women’s independence,politics,and law.The drama has been remade in China,Japan,and South Korea.This research aims to use Nida’s Functional Equivalence Theory to analyze the methods of its English-to-Chinese subtitle translation by considering social,cultural,and historic backgrounds between China and America.After data collection and case analysis,the study found that:(1)Five major translation methods are adopted in the subtitle translation of The Good Wife.They are free translation,variation,literal translation,addition,and omission.Among them,free translation is the most frequently used,while omission is used least.(2)The subtitle translation of films and TV series is limited by time and space restrictions,social-cultural differences,and other factors.When translating,translators should try to use humorous words,euphemism,intonation,and other ways,and combine different methods such as literal translation,free translation,variation,addition,omission,and other methods to seek equivalence both in the meaning and function of subtitles under the guidance of Functional Equivalence Theory.
文摘This paper studies high order compact finite volume methods on non-uniform meshes for one-dimensional elliptic and parabolic differential equations with the Robin boundary conditions.An explicit scheme and an implicit scheme are obtained by discretizing the equivalent integral form of the equation.For the explicit scheme with nodal values,the algebraic system can be solved by the Thomas method.For the implicit scheme with both nodal values and their derivatives,the system can be implemented by a prediction-correction procedure,where in the correction stage,an implicit formula for recovering the nodal derivatives is introduced.Taking two point boundary value problem as an example,we prove that both the explicit and implicit schemes are convergent with fourth order accuracy with respect to some standard discrete norms using the energy method.Two numerical examples demonstrate the correctness and effectiveness of the schemes,as well as the indispensability of using non-uniform meshes.
文摘Objective: To analyze the advantages of diversified teaching methods in the clinical instruction of operating room nursing interns. Methods: Twenty-one nursing interns who underwent internships in the operating room from March 2023 to March 2024 were selected as the control group and received conventional teaching methods. Another twenty-one nursing interns who underwent internships in the operating room from April 2024 to April 2025 were selected as the experimental group and received diversified teaching methods. The teaching effects of the two groups were compared. Results: The experimental group scored higher than the control group in assessments, teaching satisfaction, and teaching quality evaluations, as well as in post-instruction professional identity scores (p < 0.05). Conclusion: Diversified teaching methods can enhance the professional competence of operating room nursing interns, cultivate their professional identity, and yield high teaching satisfaction, thereby improving teaching quality.
基金Supported by Innovation Project of Hebei Academy of Agricultural and Forestry Sciences(2022KJCXZX-CGS-7)Hebei Agriculture Research System(HBCT2024170406).
文摘This paper presents an overview of the pathogens, symptoms of damage, patterns of occurrence, and chemical control methods associated with four major pear tree diseases: pear scab, pear ring rot, pear anthracnose, and pear speckle. The objective is to provide valuable references for the scientific and precise prevention and management of diseases in pear orchards, thereby contributing to the production of high-quality and high-yield pear fruits.
基金supported by the National Natural Science Foundation of China(Grant Nos.52021005,52325904,and 51991391)。
文摘Geological prospecting and the identification of adverse geological features are essential in tunnel construction,providing critical information to ensure safety and guide engineering decisions.As tunnel projects extend into deeper and more mountainous terrains,engineers face increasingly complex geological conditions,including high water pressure,intense geo-stress,elevated geothermal gradients,and active fault zones.These conditions pose substantial risks such as high-pressure water inrush,largescale collapses,and tunnel boring machine(TBM)blockages.Addressing these challenges requires advanced detection technologies capable of long-distance,high-precision,and intelligent assessments of adverse geology.This paper presents a comprehensive review of recent advancements in tunnel geological ahead prospecting methods.It summarizes the fundamental principles,technical maturity,key challenges,development trends,and real-world applications of various detection techniques.Airborne and semi-airborne geophysical methods enable large-scale reconnaissance for initial surveys in complex terrain.Tunnel-and borehole-based approaches offer high-resolution detection during excavation,including seismic ahead prospecting(SAP),TBM rock-breaking source seismic methods,fulltime-domain tunnel induced polarization(TIP),borehole electrical resistivity,and ground penetrating radar(GPR).To address scenarios involving multiple,coexisting adverse geologies,intelligent inversion and geological identification methods have been developed based on multi-source data fusion and artificial intelligence(AI)techniques.Overall,these advances significantly improve detection range,resolution,and geological characterization capabilities.The methods demonstrate strong adaptability to complex environments and provide reliable subsurface information,supporting safer and more efficient tunnel construction.
基金Supported by National Natural Science Foundation of China(31302071)Special Fund for Agro-scientific Research in the Public Interest(201303046)+1 种基金Jiangsu Agricultural Science and Technology Innovation Fund(CX(14)2045)"333 High-level Personnel Training Project"of Jiangsu Province(BRA2012194)~~
文摘[Objective] In this study, the quantitive detection of PCV2 （porcine circovirus type 2） in vitro was achieved. We aimed to establish two kinds of TaqMan real-time PCR methods based on PCV20RF1 and ORF2 respectively and compare them. [Method] According to the relatively＇conserved sequences of PCV20RF1 and ORF2 registered in GenBank, two pairs of specific primers and TaqMan probes were designed and synthesized. Then the recombinant plasmids containing the whole sequences of PCV20RF1 and ORF2 were constructed to draw the standard curves through optimizing the reaction system and conditions. And thus two kinds of TaqMan real-time PCR detection methods based on the whole sequences of ORF1 and ORF2 respectively were constructed for PCV2. [Result] For the two established standard curves, the Ct values showed a good linear relationship with the loga- rithms of copy numbers of templates （F2〉0.99）. The amplification efficiency ranged from 90% to 110%. The amplifications all had a good repeatability with variation coefficients within groups all less than 5%. Moreover, the amplifications all had a good specificity. When the sequences of porcine parvovirus （PPV）, porcine circovirus type 1 （PCV1）, swine pseudorabies virus （PRV）, porcine reproductive and respiratory syndrome virus （PRRSV） were used as templates, the target sequence was not amplified. The amplifications also had a high sensitivity. The ORF1 detection method could reach 1.0x10T copies/;ul, and the ORF2 detection method could reach 1.0×10^2 copies/μl. The two established real-time PCR detection methods were used to detect the 80 clinical samples respectively. The results showed the magnitudes of 72 amplified samples were basically consistent between the 2 detection methods, while the magnitudes of the other 8 amplified samples were inconsistent. Then the 8 samples were detected with SYBR Green I real-time PCR method established based on the sequence of PCV2-1ike factor P1 by Wen et aL The PCV2-1ike factor P1 was amplified in all the 8 samples, indicating the 8 samples were all infected with PCV2-1ike factor P1. [Conclusion] The ORFl-based detection method has a higher accuracy, and it can be used for the rapid detection of PCV2.
