Within the CNS nuclear factor-kappa B（NF-κB） transcription factors are involved in a wide range of functions both in homeostasis and in pathology.Over the years,our and other groups produced a vast array of informa...Within the CNS nuclear factor-kappa B（NF-κB） transcription factors are involved in a wide range of functions both in homeostasis and in pathology.Over the years,our and other groups produced a vast array of information on the complex involvement of NF-κB proteins in different aspects of postnatal neurogenesis In particular,several extracellular signals and membrane receptors have been identified as being able to affect neural progenitor cells（NPC） and their progeny via NF-κB activation.A crucial role in the regulation of neuronal fate specification in adult hippocampal NPC is played by the NF-κB p50 subunit.NF-κB p50 KO mice display a remarkable reduction in adult hippocampal neurogenesis which correlates with a selective defect in hippocampal-dependent short-term memory.Moreover absence of NF-κB p50 can profoundly affect the in vitro proneurogenic response of adult hippocampal NPC（ahN PC） to several endogenous signals and drugs.Herein we briefly review the current knowledge on the pivotal role of NF-κB p50 in the regulation of adult hippocampal neurogenesis.In addition we discuss more recent data that further extend the relevance of NF-κB p50 to novel astroglia-derived signals which can influence neuronal specification of ahN PC and to astrocyte-NPC cross-talk.展开更多
基金supported by a grant from Fondazi one Cariplo to MG
文摘Within the CNS nuclear factor-kappa B（NF-κB） transcription factors are involved in a wide range of functions both in homeostasis and in pathology.Over the years,our and other groups produced a vast array of information on the complex involvement of NF-κB proteins in different aspects of postnatal neurogenesis In particular,several extracellular signals and membrane receptors have been identified as being able to affect neural progenitor cells（NPC） and their progeny via NF-κB activation.A crucial role in the regulation of neuronal fate specification in adult hippocampal NPC is played by the NF-κB p50 subunit.NF-κB p50 KO mice display a remarkable reduction in adult hippocampal neurogenesis which correlates with a selective defect in hippocampal-dependent short-term memory.Moreover absence of NF-κB p50 can profoundly affect the in vitro proneurogenic response of adult hippocampal NPC（ahN PC） to several endogenous signals and drugs.Herein we briefly review the current knowledge on the pivotal role of NF-κB p50 in the regulation of adult hippocampal neurogenesis.In addition we discuss more recent data that further extend the relevance of NF-κB p50 to novel astroglia-derived signals which can influence neuronal specification of ahN PC and to astrocyte-NPC cross-talk.
文摘目的:探讨健脾化瘀解毒方通过抑制细胞焦亡防治胃癌前病变的作用机制。方法:构建胃癌前病变小鼠模型和细胞焦亡模型,将小鼠随机分为空白组,模型组,健脾化瘀解毒方高剂量组(15 g/kg)、低剂量组(7.5 g/kg)和维酶素组(0.2 g/kg),每组10只。分别进行干预后,观察小鼠胃黏膜组织病理变化及焦亡相关分子表达情况。细胞分为空白组、模型组和中药组,空白组与模型组给予空白血清,中药组给予含药血清干预后,除空白组外先后予以LPS、ATP处理。观察焦亡相关分子表达情况。结果:与空白组比较,模型组小鼠胃黏膜NLRP3、GSDMD、HMGB1表达显著升高(P<0.01),健脾化瘀解毒方高、低剂量组可显著降低其表达(P<0.01)。与空白组比较,模型组细胞焦亡关键分子NLRP3、GSDMD、Caspase-1 mRNA和蛋白表达水平升高(P<0.01,P<0.05),I L-1β、I L-18 m R NA表达水平显著升高(P<0.01),中药组可显著降低其表达水平(P<0.01,P<0.05)。结论:健脾化瘀解毒方可通过抑制细胞焦亡防治胃癌前病变。
