Objective: To examine the proapoptotic properties of Oroxylum indicum methanol extract on cervical cancer cells. Methods: Methylene blue assay was used to determine the IC50 value of the extract. Western blotting assa...Objective: To examine the proapoptotic properties of Oroxylum indicum methanol extract on cervical cancer cells. Methods: Methylene blue assay was used to determine the IC50 value of the extract. Western blotting assays were done to analyze the expression of HPV oncoproteins (HPV18 E6 and E7) and apoptotic molecules (caspase-3 and caspase-8). Reverse transcriptase PCR assays were performed to determine genetic alteration of tumor suppressors p53 and pRb and apoptosis markers Fas and FasL. Enzyme-linked immunosorbent assay (ELISA) was done to determine the expression of cytokine levels (IL-6 and IL-12). Results: The determination of IC50 value indicated a higher anti-proliferative activity of the extract compared to cisplatin. After 24 hours of treatment, Western blot analysis showed that treated HeLa cells exhibited a significant down-regulation of HPV18 oncoproteins E6 and E7, and a significant induction of caspase-8 and caspase-3 activation level. Meanwhile, the mRNA expressions of p53, pRb, Fas and FasL were significantly upregulated in treated cells. Moreover, ELISA showed an increased IL-12 and decreased IL-6 production after Oroxylum indicum treatment. Conclusions: The methanol extract of Oroxylum indicum has an anti-proliferative activity and proapoptotic potential. It induces localized-immunity improvements by altering cytokine production in HPV-positive cervical cancer cells.展开更多
Objective:To investigate the effect of Oroxylum indicum fruit extract on high-fat diet-induced hyperlipidemic mice.Methods:The phytochemical composition of Oroxylum indicum fruit extract was determined by liquid chrom...Objective:To investigate the effect of Oroxylum indicum fruit extract on high-fat diet-induced hyperlipidemic mice.Methods:The phytochemical composition of Oroxylum indicum fruit extract was determined by liquid chromatographymass spectrometry/mass spectrometry(LC-MS/MS)and gas chromatography-mass spectrometry.Forty-two male mice were used.The mice were divided into six groups:normal control,high-fat diet control,simvastatin treatment(20 mg/kg BW/day),and Oroxylum indicum fruit extract(100,200,300 mg/kg BW/day)treatment groups.Food intake,body weight,serum parameters,lipid profile,and histopathological lesions of the kidney,liver,and epididymal fat were observed.Results:LC-MS/MS results revealed four major components of Oroxylum indicum fruit extract:luteolin,apigenin,baicalein,and oroxylin A.Twenty-seven volatile oils were identified from Oroxylum indicum fruit extract.Daily oral administration of Oroxylum indicum fruit extract at 100 to 300 mg/kg BW/day significantly reduced the body weight,total cholesterol,triglyceride,and low-density lipoprotein cholesterol level(P<0.05),whereas high-density lipoprotein cholesterol was higher than the high-fat diet control group.Treatment with 300 mg/kg BW/day Oroxylum indicum fruit extract reduced the pathological lesion and prevented fat accumulation in the kidney and liver.Conclusions:Oroxylum indicum fruit extract has hypolipidemic effect in hyperlipidemic mice,and the active ingredients of Oroxylum indicum fruit extract,both flavonoids and volatile oils,should be further explored as an antihyperlipidemic agent.展开更多
In this study,we investigated how Oroxylum indicum leaf and fruit extracts affect the viability and migration of MCF-7 breast cancer cells and the mechanisms of action responsible for these effects.MCF-7 cells treated...In this study,we investigated how Oroxylum indicum leaf and fruit extracts affect the viability and migration of MCF-7 breast cancer cells and the mechanisms of action responsible for these effects.MCF-7 cells treated with the extracts were examined using the sulforhodamine B,colony formation and caspase 3 activity assays,and by Western blotting.O.indicum extracts were found to inhibit MCF-7 cell growth in a concentration-and time-dependent manner,with 48 h IC50 values of 57.02±2.85μg/mL and 131.3±19.2μg/mL for leaf and fruit extracts,respectively.Further,the O.indicum leaf extract caused a reduction in MCF-7 cell viability,induction of MCF-7 cell apoptosis and ROS formation,and an increase in caspase 3 activity.Also,the two extracts inhibited MCF-7 cell migration and reduced both MMP 9 and ICAMP1 gene expression and MMP9 protein expression.Additionally,O.indicum extracts greatly reduced expression of the cell cycle regulatory protein Rac1 in the mevalonate pathway.In summary,O.indicum leaf and fruit extracts reduce breast cancer cell growth,cell viability and cell migration.O.indicum constituents could,therefore,be useful for augmenting the activity of chemotherapeutic drugs employed to treat breast cancer.展开更多
Urban dust contributes to air pollution,leading to allergies and airway inflammation.Herbal plants,such as Oroxylum indicum(Phe Kaa),are increasingly being explored for their immunomodulatory potential.This study inve...Urban dust contributes to air pollution,leading to allergies and airway inflammation.Herbal plants,such as Oroxylum indicum(Phe Kaa),are increasingly being explored for their immunomodulatory potential.This study investigated the chemical composition and anti-inflammatory properties of O.indicum,with a focus on the impact of traditional Thai grilling methods on flavonoid content.High-performance liquid chromatography(HPLC)was used to analyze flavonoid glycosides and aglycones.The HPLC analysis revealed increased levels of aglycone flavonoids(oroxylin A,chrysin,baicalein)in grilled fruits,with the highest total flavonoid content found in the seeds.Anti-inflammatory effects were assessed through nitric oxide(NO)inhibition,cell viability assays,and the expression of inflammatory genes in RAW 264.7 macrophages stimulated with lipopolysaccharide(LPS)and urban dust.O.indicum extracts,particularly from grilled fruits,demonstrated dose-dependent NO inhibition and significantly reduced inflammatory gene expression,including inducible nitric oxide synthase(iNOS),cyclooxygenase-2(COX-2),interleukin-1β(IL-1β),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),and 5-lipoxygenase(5-LOX).These findings suggest that O.indicum,especially its grilled fruits,may serve as a promising anti-inflammatory supplement for managing chronic inflammation and pollution-induced respiratory issues.However,further in vivo studies are recommended to confirm these findings.展开更多
Oroxylum indicum(L.)Kurz is traditionally used across Northeast India in both culinary and medicinal applications.Its flowers and fruits are consumed as seasonal vegetables,while other parts,such as the stem bark,are ...Oroxylum indicum(L.)Kurz is traditionally used across Northeast India in both culinary and medicinal applications.Its flowers and fruits are consumed as seasonal vegetables,while other parts,such as the stem bark,are incorporated into herbal remedies to treat various ailments.In this study,the stem bark-commonly used in Assam for antimalarial preparations-was selected for in vitro evaluation of anti-plasmodial activity,alongside phytochemical characterization.Gas chromatography-mass spectrometry(GC-MS)and high-resolution mass spectrometry(HRMS)analyses of the hydroalcoholic extract revealed the presence of multiple phytoconstituents.The extract(OIHA)and two major compounds,Chrysin and Baicalein,were tested against the chloroquinesensitive strain Plasmodium falciparum 3D7(Pf3D7).While the extract exhibited limited activity,Chrysin and Baicalein demonstrated significant anti-plasmodial effects,with IC50 values of 5.92±2.69μg/mL and 12.32±1.24μg/mL,respectively.Hemolysis assays indicated greater biocompatibility of the extract compared to the isolated compounds.In silico studies further supported their potential by revealing multi-target interactions with P.falciparum proteins.These findings provide scientific support for the traditional use of O.indicum(L.)Kurz bark as an antimalarial remedy and highlight the plant’s dual role as both a food and medicinal resource.展开更多
The demand for sustainable extraction methods has led to the development of Natural Deep Eutectic Solvents(NADES)as efficient alternatives to enhance the yield of bioactive compounds from plants.This study evaluated O...The demand for sustainable extraction methods has led to the development of Natural Deep Eutectic Solvents(NADES)as efficient alternatives to enhance the yield of bioactive compounds from plants.This study evaluated Oroxylum indicum fruit as a source of baicalin,employing NADES and ultrasound-assisted extraction(UAE)to improve the efficiency.Among the 14 NADES formulations,NPBC(L-proline,1,4-butanediol,and choline chloride)achieved the highest baicalein yield.Optimization through response surface methodology identified the ideal conditions-37 min,65℃,and 40%water-resulting in a baicalein yield over 2.5 times greater than with 70%ethanol.Bioactivity assays,including DPPH and LPS-stimulated RAW macrophage tests,demonstrated that the NPBC extract had potent antioxidant and anti-inflammatory effects,significantly inhibiting nitric oxide(NO)production and downregulating inflammatory genes(iNOS,IL-6,COX-2,5-LOX)to baseline levels.This NADES-UAE approach markedly enhances the extraction efficiency and converts baicalin to baicalein,boosting the pharmacological potential of plant extracts while providing a sustainable alternative to traditional methods.展开更多
基金supported by Fundamental Research Grant Scheme Grant 203/PPSK/6171191
文摘Objective: To examine the proapoptotic properties of Oroxylum indicum methanol extract on cervical cancer cells. Methods: Methylene blue assay was used to determine the IC50 value of the extract. Western blotting assays were done to analyze the expression of HPV oncoproteins (HPV18 E6 and E7) and apoptotic molecules (caspase-3 and caspase-8). Reverse transcriptase PCR assays were performed to determine genetic alteration of tumor suppressors p53 and pRb and apoptosis markers Fas and FasL. Enzyme-linked immunosorbent assay (ELISA) was done to determine the expression of cytokine levels (IL-6 and IL-12). Results: The determination of IC50 value indicated a higher anti-proliferative activity of the extract compared to cisplatin. After 24 hours of treatment, Western blot analysis showed that treated HeLa cells exhibited a significant down-regulation of HPV18 oncoproteins E6 and E7, and a significant induction of caspase-8 and caspase-3 activation level. Meanwhile, the mRNA expressions of p53, pRb, Fas and FasL were significantly upregulated in treated cells. Moreover, ELISA showed an increased IL-12 and decreased IL-6 production after Oroxylum indicum treatment. Conclusions: The methanol extract of Oroxylum indicum has an anti-proliferative activity and proapoptotic potential. It induces localized-immunity improvements by altering cytokine production in HPV-positive cervical cancer cells.
文摘Objective:To investigate the effect of Oroxylum indicum fruit extract on high-fat diet-induced hyperlipidemic mice.Methods:The phytochemical composition of Oroxylum indicum fruit extract was determined by liquid chromatographymass spectrometry/mass spectrometry(LC-MS/MS)and gas chromatography-mass spectrometry.Forty-two male mice were used.The mice were divided into six groups:normal control,high-fat diet control,simvastatin treatment(20 mg/kg BW/day),and Oroxylum indicum fruit extract(100,200,300 mg/kg BW/day)treatment groups.Food intake,body weight,serum parameters,lipid profile,and histopathological lesions of the kidney,liver,and epididymal fat were observed.Results:LC-MS/MS results revealed four major components of Oroxylum indicum fruit extract:luteolin,apigenin,baicalein,and oroxylin A.Twenty-seven volatile oils were identified from Oroxylum indicum fruit extract.Daily oral administration of Oroxylum indicum fruit extract at 100 to 300 mg/kg BW/day significantly reduced the body weight,total cholesterol,triglyceride,and low-density lipoprotein cholesterol level(P<0.05),whereas high-density lipoprotein cholesterol was higher than the high-fat diet control group.Treatment with 300 mg/kg BW/day Oroxylum indicum fruit extract reduced the pathological lesion and prevented fat accumulation in the kidney and liver.Conclusions:Oroxylum indicum fruit extract has hypolipidemic effect in hyperlipidemic mice,and the active ingredients of Oroxylum indicum fruit extract,both flavonoids and volatile oils,should be further explored as an antihyperlipidemic agent.
基金financially supported by Mahasarakham University(Fast Track 2019)。
文摘In this study,we investigated how Oroxylum indicum leaf and fruit extracts affect the viability and migration of MCF-7 breast cancer cells and the mechanisms of action responsible for these effects.MCF-7 cells treated with the extracts were examined using the sulforhodamine B,colony formation and caspase 3 activity assays,and by Western blotting.O.indicum extracts were found to inhibit MCF-7 cell growth in a concentration-and time-dependent manner,with 48 h IC50 values of 57.02±2.85μg/mL and 131.3±19.2μg/mL for leaf and fruit extracts,respectively.Further,the O.indicum leaf extract caused a reduction in MCF-7 cell viability,induction of MCF-7 cell apoptosis and ROS formation,and an increase in caspase 3 activity.Also,the two extracts inhibited MCF-7 cell migration and reduced both MMP 9 and ICAMP1 gene expression and MMP9 protein expression.Additionally,O.indicum extracts greatly reduced expression of the cell cycle regulatory protein Rac1 in the mevalonate pathway.In summary,O.indicum leaf and fruit extracts reduce breast cancer cell growth,cell viability and cell migration.O.indicum constituents could,therefore,be useful for augmenting the activity of chemotherapeutic drugs employed to treat breast cancer.
基金supported by the Fundamental Fund(FF)of Khon Kaen University under the National Science,Research,and Innovation Fund(NSRF),Thailand.
文摘Urban dust contributes to air pollution,leading to allergies and airway inflammation.Herbal plants,such as Oroxylum indicum(Phe Kaa),are increasingly being explored for their immunomodulatory potential.This study investigated the chemical composition and anti-inflammatory properties of O.indicum,with a focus on the impact of traditional Thai grilling methods on flavonoid content.High-performance liquid chromatography(HPLC)was used to analyze flavonoid glycosides and aglycones.The HPLC analysis revealed increased levels of aglycone flavonoids(oroxylin A,chrysin,baicalein)in grilled fruits,with the highest total flavonoid content found in the seeds.Anti-inflammatory effects were assessed through nitric oxide(NO)inhibition,cell viability assays,and the expression of inflammatory genes in RAW 264.7 macrophages stimulated with lipopolysaccharide(LPS)and urban dust.O.indicum extracts,particularly from grilled fruits,demonstrated dose-dependent NO inhibition and significantly reduced inflammatory gene expression,including inducible nitric oxide synthase(iNOS),cyclooxygenase-2(COX-2),interleukin-1β(IL-1β),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),and 5-lipoxygenase(5-LOX).These findings suggest that O.indicum,especially its grilled fruits,may serve as a promising anti-inflammatory supplement for managing chronic inflammation and pollution-induced respiratory issues.However,further in vivo studies are recommended to confirm these findings.
文摘Oroxylum indicum(L.)Kurz is traditionally used across Northeast India in both culinary and medicinal applications.Its flowers and fruits are consumed as seasonal vegetables,while other parts,such as the stem bark,are incorporated into herbal remedies to treat various ailments.In this study,the stem bark-commonly used in Assam for antimalarial preparations-was selected for in vitro evaluation of anti-plasmodial activity,alongside phytochemical characterization.Gas chromatography-mass spectrometry(GC-MS)and high-resolution mass spectrometry(HRMS)analyses of the hydroalcoholic extract revealed the presence of multiple phytoconstituents.The extract(OIHA)and two major compounds,Chrysin and Baicalein,were tested against the chloroquinesensitive strain Plasmodium falciparum 3D7(Pf3D7).While the extract exhibited limited activity,Chrysin and Baicalein demonstrated significant anti-plasmodial effects,with IC50 values of 5.92±2.69μg/mL and 12.32±1.24μg/mL,respectively.Hemolysis assays indicated greater biocompatibility of the extract compared to the isolated compounds.In silico studies further supported their potential by revealing multi-target interactions with P.falciparum proteins.These findings provide scientific support for the traditional use of O.indicum(L.)Kurz bark as an antimalarial remedy and highlight the plant’s dual role as both a food and medicinal resource.
基金funding support from the NSRF via the Program Management Unit for Human Resources&Institutional Development,Research and Innovation(PMU-B)[grant number B13F670054].
文摘The demand for sustainable extraction methods has led to the development of Natural Deep Eutectic Solvents(NADES)as efficient alternatives to enhance the yield of bioactive compounds from plants.This study evaluated Oroxylum indicum fruit as a source of baicalin,employing NADES and ultrasound-assisted extraction(UAE)to improve the efficiency.Among the 14 NADES formulations,NPBC(L-proline,1,4-butanediol,and choline chloride)achieved the highest baicalein yield.Optimization through response surface methodology identified the ideal conditions-37 min,65℃,and 40%water-resulting in a baicalein yield over 2.5 times greater than with 70%ethanol.Bioactivity assays,including DPPH and LPS-stimulated RAW macrophage tests,demonstrated that the NPBC extract had potent antioxidant and anti-inflammatory effects,significantly inhibiting nitric oxide(NO)production and downregulating inflammatory genes(iNOS,IL-6,COX-2,5-LOX)to baseline levels.This NADES-UAE approach markedly enhances the extraction efficiency and converts baicalin to baicalein,boosting the pharmacological potential of plant extracts while providing a sustainable alternative to traditional methods.
