Background:Urinary tract infections(UTIs),primarily caused by uropathogenic Escherichia coli(UPEC),are a significant global health concern.The complications arise from antibiotic resistance and biofilm formation,which...Background:Urinary tract infections(UTIs),primarily caused by uropathogenic Escherichia coli(UPEC),are a significant global health concern.The complications arise from antibiotic resistance and biofilm formation,which reduce the effectiveness of conventional treatments.This study aims to evaluate the antibiofilm activity of the homeopathic medicine Terebinthinae oleum in potencies 30C,200C,and 1M against UTI-causing E.coli,and to compare its effectiveness with the standard antibiotic Gentamycin.Methods:An in-vitro biofilm model was employed.E.coli biofilms were cultivated in microtiter plates and treated with Terebinthinae oleum(30C,200C,1M)and Gentamycin.Biofilm biomass was assessed through crystal violet staining,and optical density(OD)was measured using an ELISA microplate reader.Results:Among the tested potencies,Terebinthinae oleum 30C showed the most prominent inhibitory activity on E.coli biofilms.The inhibition percentage was compared with Gentamycin as a control.Terebinthinae oleum 30C demonstrated 41.88%inhibition of biofilm biomass,while Gentamycin exhibited up to 78.98%inhibition.Higher potencies of Terebinthinae oleum(200C and 1M)showed reduced activity(30.15%and 24.81%,respectively).Conclusion:Terebinthinae oleum,especially at 30C potency,exhibits measurable antibiofilm activity against E.coli,although less effective than Gentamycin.These findings support its use as a complementary therapy in managing biofilm-associated UTIs,justifying further clinical and immunological research.展开更多
Oleum azadirachti consists of the oil obtained from dried seeds of Azadirachta indica A.Juss.(family:Meliaceae).Local names of Azadirachta indica A.Juss.are Abodua,aforo-oyinbo,anwe egyane,arista,azad dirakht,azadarak...Oleum azadirachti consists of the oil obtained from dried seeds of Azadirachta indica A.Juss.(family:Meliaceae).Local names of Azadirachta indica A.Juss.are Abodua,aforo-oyinbo,anwe egyane,arista,azad dirakht,azadarakht,azedarach and bead tree.Indigenous to India,and widely distributed in South and South-East Asia and cultivated in Africa,the South Pacific Islands,South and Central America and Australia,and in southern Florida and California,United States of America,it is a straight-boled deciduous tree,which is 6-25 m high.Bark is dark-brown,externally fissured with a buff inner surface and fibrous fracture,Leaves alternately arranged,pinnately compound and up to 40 cm long,and composed of 8-18 short-petiolate narrow-ovate,pointed and curved toothed leaflets,3-10 cm long and 1-4 cm wide arranged in alternate pairs.The major constituents are oxidized tetranortriterpenes including azadirachtin(azadirachtin A),azadiriadione,epoxyazadiradione,azadirone,nimbidin,nimbin,deacelylnimbin,salannin,gedunin,mahmoodin,17-hydroxydiradione and related derivatives.It is of various medicinal uses,such as a contraceptive for intravaginal use,a mosquito repellent,and treatment of vaginal infections,treatment of gastric ulcers,cardiovascular disease,malaria,rheumatism and skin disorders,external applications for treatment of septic wounds,ulcers and boils,treatment of allergic skin reactions,asthma,bruises,colic,conjunctivitis,dysmenorrhoea,fever,gout,headache,itching due to varicella,kidney stones,leukorrhoea,psoriasis,scabies,sprains and muscular pain,and wounds.It is also used as an emmenagogue.tonic,stomatic and vermicide.In conclusion,the plant oil had antifertility,antihyperglycaemic,anti-inflammatory,antimicrobial,antiviral,antiulcer,estrogenic,immune,contraceptive,autibacterial,insect repellent,and skin treatment effects.展开更多
文摘Background:Urinary tract infections(UTIs),primarily caused by uropathogenic Escherichia coli(UPEC),are a significant global health concern.The complications arise from antibiotic resistance and biofilm formation,which reduce the effectiveness of conventional treatments.This study aims to evaluate the antibiofilm activity of the homeopathic medicine Terebinthinae oleum in potencies 30C,200C,and 1M against UTI-causing E.coli,and to compare its effectiveness with the standard antibiotic Gentamycin.Methods:An in-vitro biofilm model was employed.E.coli biofilms were cultivated in microtiter plates and treated with Terebinthinae oleum(30C,200C,1M)and Gentamycin.Biofilm biomass was assessed through crystal violet staining,and optical density(OD)was measured using an ELISA microplate reader.Results:Among the tested potencies,Terebinthinae oleum 30C showed the most prominent inhibitory activity on E.coli biofilms.The inhibition percentage was compared with Gentamycin as a control.Terebinthinae oleum 30C demonstrated 41.88%inhibition of biofilm biomass,while Gentamycin exhibited up to 78.98%inhibition.Higher potencies of Terebinthinae oleum(200C and 1M)showed reduced activity(30.15%and 24.81%,respectively).Conclusion:Terebinthinae oleum,especially at 30C potency,exhibits measurable antibiofilm activity against E.coli,although less effective than Gentamycin.These findings support its use as a complementary therapy in managing biofilm-associated UTIs,justifying further clinical and immunological research.
基金Supported by a grant from advanced medical and dental(AMDI)/Universiti Sains Malaysia(USM)(FWA/IRB Registration No:00005528/00002294)
文摘Oleum azadirachti consists of the oil obtained from dried seeds of Azadirachta indica A.Juss.(family:Meliaceae).Local names of Azadirachta indica A.Juss.are Abodua,aforo-oyinbo,anwe egyane,arista,azad dirakht,azadarakht,azedarach and bead tree.Indigenous to India,and widely distributed in South and South-East Asia and cultivated in Africa,the South Pacific Islands,South and Central America and Australia,and in southern Florida and California,United States of America,it is a straight-boled deciduous tree,which is 6-25 m high.Bark is dark-brown,externally fissured with a buff inner surface and fibrous fracture,Leaves alternately arranged,pinnately compound and up to 40 cm long,and composed of 8-18 short-petiolate narrow-ovate,pointed and curved toothed leaflets,3-10 cm long and 1-4 cm wide arranged in alternate pairs.The major constituents are oxidized tetranortriterpenes including azadirachtin(azadirachtin A),azadiriadione,epoxyazadiradione,azadirone,nimbidin,nimbin,deacelylnimbin,salannin,gedunin,mahmoodin,17-hydroxydiradione and related derivatives.It is of various medicinal uses,such as a contraceptive for intravaginal use,a mosquito repellent,and treatment of vaginal infections,treatment of gastric ulcers,cardiovascular disease,malaria,rheumatism and skin disorders,external applications for treatment of septic wounds,ulcers and boils,treatment of allergic skin reactions,asthma,bruises,colic,conjunctivitis,dysmenorrhoea,fever,gout,headache,itching due to varicella,kidney stones,leukorrhoea,psoriasis,scabies,sprains and muscular pain,and wounds.It is also used as an emmenagogue.tonic,stomatic and vermicide.In conclusion,the plant oil had antifertility,antihyperglycaemic,anti-inflammatory,antimicrobial,antiviral,antiulcer,estrogenic,immune,contraceptive,autibacterial,insect repellent,and skin treatment effects.
