Polynyas and their adjacent seasonal ice zones(SIZs)represent the most productive regions in the Southern Ocean,supporting unique food webs that are highly sensitive to climate change.Understanding the dynamics of phy...Polynyas and their adjacent seasonal ice zones(SIZs)represent the most productive regions in the Southern Ocean,supporting unique food webs that are highly sensitive to climate change.Understanding the dynamics of phytoplankton and the carbon pool in these areas is crucial for assessing the role of the Southern Ocean in global carbon cycling.During the late stage of an algal bloom,seawater samples at 14 stations were collected in the Amundsen Sea Polynya(ASP)and adjacent SIZ.Using nutrients,phytoplankton pigments,organic carbon(OC),remote sensing data,and physicochemical measurements,as well as CHEMTAX model simulations,we investigated the response of the phytoplankton crops,taxonomic composition,and OC pool to environmental factors.Our analyses revealed that hydrodynamic regimes of the polynya,adjacent SIZs and open sea were regulated by the regionally varying intrusion of Circumpolar Deep Water,photosynthetically active radiation and sea ice melt water.The ASP exhibited the highest seasonal nutrient utilization rates[ΔN=(1059±386)mmol/m^(2),ΔP=(50±17)mmol/m^(2) andΔSi=(956±904)mmol/m^(2)],while the open sea had lower rates.The integrated chlorophyll a(Chl a)concentration at depths of 0–200 m ranged from 20.4 mg/m^(2) to 1420.0 mg/m^(2) and peaked in the polynya.In the study area,Haptophytes Phaeocystis antarctica was the dominant functional group(34%±27%),and diatoms acted as a secondary contributor(23%±14%).The major functional group and particulate OC(POC)contributor varied from diatoms(36%±12%)in the open sea to haptophytes(48%±31%)in the polynya waters.Strong light conditions and microelement limitations promoted the dominance of P.antarctica(low Fe forms)dominance in the ASP.The strong correlations between the POC and Chl a depth-integrated concentration suggest that the POC was primarily derived from phytoplankton,while dissolved OC(DOC)was influenced by consumer activity and water mass transport.In addition,the transport of OC in the upper 200 m of the water column within the ASP was quantified,revealing the predominantly westward fluxes for both DOC[9.0 mg/(m^(2)·s)]and POC[7.2 mg/(m^(2)·s)].The latitudinal transport exhibited the northward transport of DOC[8.1 mg/(m^(2)·s)]and southward transport of POC[4.3 mg/(m^(2)·s)]movement.These findings have significant implications for enhancing our understanding of how hydrodynamics influence OC cycling in polynya regions.展开更多
Background:Ovarian cancer(OC)is a representative malignancy of the female reproductive system,with a poor prognosis.Long non-coding RNAs(lncRNAs)crucially affect tumor development.This study aimed to identify lncRNAs ...Background:Ovarian cancer(OC)is a representative malignancy of the female reproductive system,with a poor prognosis.Long non-coding RNAs(lncRNAs)crucially affect tumor development.This study aimed to identify lncRNAs that potentially participated in OC.Methods:LncRNA expression in cells and tissues was quantified using reverse transcription-quantitative PCR,while fluorescence in situ hybridization determined their cellular localization.Various in vitro assays,together with a mouse xenograft model,were employed to elucidate the function of CYMP antisense RNA 1(CYMP-AS1)in OC.The molecular mechanisms underlying CYMP-AS1 regulation were investigated through RNA pull-down and immunoprecipitation assays,immunofluorescence staining,western blotting,and mRNA stability assays.Results:This study identified a previously unreported lncRNA,CYMP-AS1,which exhibits increased expression in the cytoplasm of OC tissues and cells.Knockout of CYMP-AS1 reduced the OC cell proliferation,migration,invasion,and epithelial-mesenchymal transition(EMT).CYMP-AS1 directly interacts with heterogeneous nuclear ribonucleoprotein M(hnRNPM),inducing its intracellular translocation and reducing the stability of Axis inhibition protein 2(AXIN2)mRNA.This process ultimately elevated the expression of Wnt/β-catenin signaling pathway-related proteins.Conclusion:This study confirms CYMP-AS1 as a novel biomarker in OC progression and suggests that the CYMP-AS1/hnRNPM/AXIN2 axis may offer an innovative strategy for OC treatment.展开更多
耕升追风系列迎来扩容,推出了耕升GeForce RTX 5090 D v2追风OC这一新旗舰。这款新旗舰显卡延续了追风系列一贯的简约风格,在外观上没有过多“花哨”的装饰,却凭借扎实的做工和出色的性能表现展现出它的魅力--其散热系统经过精心设计,...耕升追风系列迎来扩容,推出了耕升GeForce RTX 5090 D v2追风OC这一新旗舰。这款新旗舰显卡延续了追风系列一贯的简约风格,在外观上没有过多“花哨”的装饰,却凭借扎实的做工和出色的性能表现展现出它的魅力--其散热系统经过精心设计,能够有效应对高负载运行时的热量问题,确保显卡在长时间使用过程中保持稳定的性能输出。同时,耕升GeForce RTX 5090 D v2追风OC在核心参数上有着亮眼的表现,强悍性能能够满足各类高端游戏和专业图形应用的需求,为用户带来不俗的体验。展开更多
基金The National Polar Special Program under contract Nos IRASCC 01-01-02 and IRASCC 02-02the National Natural Science Foundation of China under contract Nos 41976228,42276255,41976227,42176227,and 42076243+1 种基金the International Cooperation Key Project of the Ministry of Science and Technology under contract No.2022YFE0136500the Scientific Research Fund of the Second Institute of Oceanography,Ministry of Natural Resources,under contract Nos JG2011,JG2211,JG2013,and JG1805.
文摘Polynyas and their adjacent seasonal ice zones(SIZs)represent the most productive regions in the Southern Ocean,supporting unique food webs that are highly sensitive to climate change.Understanding the dynamics of phytoplankton and the carbon pool in these areas is crucial for assessing the role of the Southern Ocean in global carbon cycling.During the late stage of an algal bloom,seawater samples at 14 stations were collected in the Amundsen Sea Polynya(ASP)and adjacent SIZ.Using nutrients,phytoplankton pigments,organic carbon(OC),remote sensing data,and physicochemical measurements,as well as CHEMTAX model simulations,we investigated the response of the phytoplankton crops,taxonomic composition,and OC pool to environmental factors.Our analyses revealed that hydrodynamic regimes of the polynya,adjacent SIZs and open sea were regulated by the regionally varying intrusion of Circumpolar Deep Water,photosynthetically active radiation and sea ice melt water.The ASP exhibited the highest seasonal nutrient utilization rates[ΔN=(1059±386)mmol/m^(2),ΔP=(50±17)mmol/m^(2) andΔSi=(956±904)mmol/m^(2)],while the open sea had lower rates.The integrated chlorophyll a(Chl a)concentration at depths of 0–200 m ranged from 20.4 mg/m^(2) to 1420.0 mg/m^(2) and peaked in the polynya.In the study area,Haptophytes Phaeocystis antarctica was the dominant functional group(34%±27%),and diatoms acted as a secondary contributor(23%±14%).The major functional group and particulate OC(POC)contributor varied from diatoms(36%±12%)in the open sea to haptophytes(48%±31%)in the polynya waters.Strong light conditions and microelement limitations promoted the dominance of P.antarctica(low Fe forms)dominance in the ASP.The strong correlations between the POC and Chl a depth-integrated concentration suggest that the POC was primarily derived from phytoplankton,while dissolved OC(DOC)was influenced by consumer activity and water mass transport.In addition,the transport of OC in the upper 200 m of the water column within the ASP was quantified,revealing the predominantly westward fluxes for both DOC[9.0 mg/(m^(2)·s)]and POC[7.2 mg/(m^(2)·s)].The latitudinal transport exhibited the northward transport of DOC[8.1 mg/(m^(2)·s)]and southward transport of POC[4.3 mg/(m^(2)·s)]movement.These findings have significant implications for enhancing our understanding of how hydrodynamics influence OC cycling in polynya regions.
基金funded by the National Natural Science Foundation of China(No.8207286).
文摘Background:Ovarian cancer(OC)is a representative malignancy of the female reproductive system,with a poor prognosis.Long non-coding RNAs(lncRNAs)crucially affect tumor development.This study aimed to identify lncRNAs that potentially participated in OC.Methods:LncRNA expression in cells and tissues was quantified using reverse transcription-quantitative PCR,while fluorescence in situ hybridization determined their cellular localization.Various in vitro assays,together with a mouse xenograft model,were employed to elucidate the function of CYMP antisense RNA 1(CYMP-AS1)in OC.The molecular mechanisms underlying CYMP-AS1 regulation were investigated through RNA pull-down and immunoprecipitation assays,immunofluorescence staining,western blotting,and mRNA stability assays.Results:This study identified a previously unreported lncRNA,CYMP-AS1,which exhibits increased expression in the cytoplasm of OC tissues and cells.Knockout of CYMP-AS1 reduced the OC cell proliferation,migration,invasion,and epithelial-mesenchymal transition(EMT).CYMP-AS1 directly interacts with heterogeneous nuclear ribonucleoprotein M(hnRNPM),inducing its intracellular translocation and reducing the stability of Axis inhibition protein 2(AXIN2)mRNA.This process ultimately elevated the expression of Wnt/β-catenin signaling pathway-related proteins.Conclusion:This study confirms CYMP-AS1 as a novel biomarker in OC progression and suggests that the CYMP-AS1/hnRNPM/AXIN2 axis may offer an innovative strategy for OC treatment.
文摘耕升追风系列迎来扩容,推出了耕升GeForce RTX 5090 D v2追风OC这一新旗舰。这款新旗舰显卡延续了追风系列一贯的简约风格,在外观上没有过多“花哨”的装饰,却凭借扎实的做工和出色的性能表现展现出它的魅力--其散热系统经过精心设计,能够有效应对高负载运行时的热量问题,确保显卡在长时间使用过程中保持稳定的性能输出。同时,耕升GeForce RTX 5090 D v2追风OC在核心参数上有着亮眼的表现,强悍性能能够满足各类高端游戏和专业图形应用的需求,为用户带来不俗的体验。
