The writer O.Henry was famous for surprising endings,which is a common feature of his short stories.They have original plots and usually end in a way that you can never imagine.Because of his humorous language,his uni...The writer O.Henry was famous for surprising endings,which is a common feature of his short stories.They have original plots and usually end in a way that you can never imagine.Because of his humorous language,his unique narrative skills,and his unexpected and reasonable ending,O.Henry got a high reputation.His execllent technique of writng is more obvious for the irony of reality and the return of human nature.The O.Henry-style ending opened up the pluralistic development of later realistic literature.The highly critical ending of its turning point also promotes the practical significance of the reaction of world literature to society.展开更多
目前大多数软件开发都采用面向对象技术,而数据仍然保存在关系数据库中。由于对象模型和关系模型存在阻抗不匹配,因此实现对象与关系的映射已成为软件开发领域的关键问题。采用O/R M app ing(对象/关系映射)技术、XM L技术和软件分层的...目前大多数软件开发都采用面向对象技术,而数据仍然保存在关系数据库中。由于对象模型和关系模型存在阻抗不匹配,因此实现对象与关系的映射已成为软件开发领域的关键问题。采用O/R M app ing(对象/关系映射)技术、XM L技术和软件分层的设计思想,实现了一个有效的数据库访问中间件,解决了利用ADO.NET开发应用程序遇到的数据表示和存取问题。展开更多
Fluorescence in situ hybridization （FISH） and comparative genomic hybridization （CGH） were applied to somatic chromosomes preparations of Oryza sativa, O. officinalis, and O. meyeriana with labeled probes of Cot-1...Fluorescence in situ hybridization （FISH） and comparative genomic hybridization （CGH） were applied to somatic chromosomes preparations of Oryza sativa, O. officinalis, and O. meyeriana with labeled probes of Cot-1 DNA and genomic DNA＇from the cultivated rice. The coverage percentage （%） and size （Mb） of Cot-1 DNA in O. sativa, O. officinalis, and O. meyeriana were 47.1 ±0.16, 38.61 ±0.13, 44.38＋_0.13, and 212.33 ± 1.21,269.42 ± 0.89, 532.56± 1.68 Mb, respectively. The coverage percentage and size of genomic DNA from O. sativa in O. officinalis and O. meyeriana were 91.0, 93.6% and 634, 1 123 Mb, respectively, in which 365 and 591 Mb in O. officinalis and O. meyeriana were from O. sativa genomic DNA, but not from repetitive sequences of O. sativa, and the uncoverage genome size in O. officinalis and O. meyeriana were 64 and 78 Mb, respectively. In addition, karyotype analysis was conducted based on the signal bands of Cot-1 DNA in O. sativa, O. officinalis, and O. meyeriana. The results showed that highly and moderately repetitive sequences in Oryza genus were conserved as the functional genes during evolution. The repetitive sequences reduplication may be one of the important causes of the genome enlargement of O. officinalis and O. meyeriana, and O. officinalis genome enlarged more slowly when compared with O. meyeriana. Based on the above results, it is concluded that O. officinalis and O. meyeriana were formed by reduplication, rearrangement, and gene selective loss during the evolution process.展开更多
文摘The writer O.Henry was famous for surprising endings,which is a common feature of his short stories.They have original plots and usually end in a way that you can never imagine.Because of his humorous language,his unique narrative skills,and his unexpected and reasonable ending,O.Henry got a high reputation.His execllent technique of writng is more obvious for the irony of reality and the return of human nature.The O.Henry-style ending opened up the pluralistic development of later realistic literature.The highly critical ending of its turning point also promotes the practical significance of the reaction of world literature to society.
文摘目前大多数软件开发都采用面向对象技术,而数据仍然保存在关系数据库中。由于对象模型和关系模型存在阻抗不匹配,因此实现对象与关系的映射已成为软件开发领域的关键问题。采用O/R M app ing(对象/关系映射)技术、XM L技术和软件分层的设计思想,实现了一个有效的数据库访问中间件,解决了利用ADO.NET开发应用程序遇到的数据表示和存取问题。
基金This work was supported by the National High Tech R ＆ D Program of China （863 Program, 2004AA227120） the Scientific Research Foundation for the Returned 0verseas Chinese Scholars, Ministry of Education of China （BZY04003）+1 种基金 China Postdoctoral Science Foundation （20040350574）the Project of Science and Technology for Youth, Wuhan, China （2004500607135）.
文摘Fluorescence in situ hybridization （FISH） and comparative genomic hybridization （CGH） were applied to somatic chromosomes preparations of Oryza sativa, O. officinalis, and O. meyeriana with labeled probes of Cot-1 DNA and genomic DNA＇from the cultivated rice. The coverage percentage （%） and size （Mb） of Cot-1 DNA in O. sativa, O. officinalis, and O. meyeriana were 47.1 ±0.16, 38.61 ±0.13, 44.38＋_0.13, and 212.33 ± 1.21,269.42 ± 0.89, 532.56± 1.68 Mb, respectively. The coverage percentage and size of genomic DNA from O. sativa in O. officinalis and O. meyeriana were 91.0, 93.6% and 634, 1 123 Mb, respectively, in which 365 and 591 Mb in O. officinalis and O. meyeriana were from O. sativa genomic DNA, but not from repetitive sequences of O. sativa, and the uncoverage genome size in O. officinalis and O. meyeriana were 64 and 78 Mb, respectively. In addition, karyotype analysis was conducted based on the signal bands of Cot-1 DNA in O. sativa, O. officinalis, and O. meyeriana. The results showed that highly and moderately repetitive sequences in Oryza genus were conserved as the functional genes during evolution. The repetitive sequences reduplication may be one of the important causes of the genome enlargement of O. officinalis and O. meyeriana, and O. officinalis genome enlarged more slowly when compared with O. meyeriana. Based on the above results, it is concluded that O. officinalis and O. meyeriana were formed by reduplication, rearrangement, and gene selective loss during the evolution process.
