Objective:Small molecules in snow chrysanthemum such as flavonoids,phenolic compounds and amino acids have been extensively investigated.No study to date has focused on water-soluble oligosaccharides.The objective of ...Objective:Small molecules in snow chrysanthemum such as flavonoids,phenolic compounds and amino acids have been extensively investigated.No study to date has focused on water-soluble oligosaccharides.The objective of this study is identification and determination of water-soluble oligosaccharides in snow chrysanthemum.Methods:The oligosaccharides in snow chrysanthemum were identified by high performance thin layer chromatography(HPTLC),liquid chromatography-mass spectrometry(LC-MS)combined MS library and methylation analysis for the first time.Subsequently the oligosaccharides were determined by high performance liquid chromatography with a charged aerosol detector(HPLC-CAD).Results:The oligosaccharides in snow chrysanthemum were identified as inulin-type fructooligosaccharides(FOS).The yield of FOS(DP3~DP13)in the first extraction was over 97.6%.The RSDs of repeatability in three sample amount levels(0.08 g,0.1 g,0.12 g)are lower than 4.8%and the RSDs of stability are less than 3.5%.The recoveries of FOS(DP3~13)were ranging from 96.9%to 105.6%.The contents of FOS(DP3~DP13)in flowers of snow chrysanthemum from different regions of China were greatly variant.Conclusion:This is the first time to identify and quantify FOS in snow chrysanthemum which is helpful for its performance in the in the fields of biomedical,agriculture and functional food industry as well as development of the quality control methods.In addition,the identification approach developed in this work can also be used for screening potential natural sources containing FOS.展开更多
目的了解新疆不同花色两色金鸡菊中花期多种元素的积累情况,全面评价其质量安全。方法本研究采用电感耦合等离子体质谱法(inductively coupled plasma mass spectrometry,ICP-MS)测定了3种花色两色金鸡菊(标记为‘LS’‘JS’和‘MS’)...目的了解新疆不同花色两色金鸡菊中花期多种元素的积累情况,全面评价其质量安全。方法本研究采用电感耦合等离子体质谱法(inductively coupled plasma mass spectrometry,ICP-MS)测定了3种花色两色金鸡菊(标记为‘LS’‘JS’和‘MS’)中18种元素含量,通过相关性统计、聚类热图以及偏最小二乘判别分析(partial least squares discriminant analysis,PLS-DA)分析,明确了不同种两色金鸡菊花期差异积累的关键元素,并对其安全性进行评价。结果3种不同花色两色金鸡菊花期4个不同阶段的多种元素存在显著差异,其中K、Ca、P、Mg的含量最高,K、Ca、P的投影变量重要性(variable importance in projection,VIP)值均大于2,是主要差异积累的元素,且这3种元素在‘LS’花期S2~S3时期含量范围高于‘JS’和‘MS’。通过对不同花色两色金鸡菊进行了初步健康风险评估,3种两色金鸡菊的花蕾及花瓣中Pb、Ni、Hg、Cd、As含量均对人体无健康风险。结论本研究明确了两色金鸡菊花多种元素的积累分布,为新疆两色金鸡菊质量标准全面评估提供了理论依据。展开更多
基金supported by the National Key Research and Development Project(SQ2018YPC170480,2018YFC1708202)the National Natural Science Foundation of China(81774009)+1 种基金the Key science and technology projects of Inner Mongolia Autonomous Region(2019ZD004)Open Project Program of Inner Mongolia Key Laboratory of Toxicant Monitoring and Toxicology,China(MDK 2019040)。
文摘Objective:Small molecules in snow chrysanthemum such as flavonoids,phenolic compounds and amino acids have been extensively investigated.No study to date has focused on water-soluble oligosaccharides.The objective of this study is identification and determination of water-soluble oligosaccharides in snow chrysanthemum.Methods:The oligosaccharides in snow chrysanthemum were identified by high performance thin layer chromatography(HPTLC),liquid chromatography-mass spectrometry(LC-MS)combined MS library and methylation analysis for the first time.Subsequently the oligosaccharides were determined by high performance liquid chromatography with a charged aerosol detector(HPLC-CAD).Results:The oligosaccharides in snow chrysanthemum were identified as inulin-type fructooligosaccharides(FOS).The yield of FOS(DP3~DP13)in the first extraction was over 97.6%.The RSDs of repeatability in three sample amount levels(0.08 g,0.1 g,0.12 g)are lower than 4.8%and the RSDs of stability are less than 3.5%.The recoveries of FOS(DP3~13)were ranging from 96.9%to 105.6%.The contents of FOS(DP3~DP13)in flowers of snow chrysanthemum from different regions of China were greatly variant.Conclusion:This is the first time to identify and quantify FOS in snow chrysanthemum which is helpful for its performance in the in the fields of biomedical,agriculture and functional food industry as well as development of the quality control methods.In addition,the identification approach developed in this work can also be used for screening potential natural sources containing FOS.
文摘目的了解新疆不同花色两色金鸡菊中花期多种元素的积累情况,全面评价其质量安全。方法本研究采用电感耦合等离子体质谱法(inductively coupled plasma mass spectrometry,ICP-MS)测定了3种花色两色金鸡菊(标记为‘LS’‘JS’和‘MS’)中18种元素含量,通过相关性统计、聚类热图以及偏最小二乘判别分析(partial least squares discriminant analysis,PLS-DA)分析,明确了不同种两色金鸡菊花期差异积累的关键元素,并对其安全性进行评价。结果3种不同花色两色金鸡菊花期4个不同阶段的多种元素存在显著差异,其中K、Ca、P、Mg的含量最高,K、Ca、P的投影变量重要性(variable importance in projection,VIP)值均大于2,是主要差异积累的元素,且这3种元素在‘LS’花期S2~S3时期含量范围高于‘JS’和‘MS’。通过对不同花色两色金鸡菊进行了初步健康风险评估,3种两色金鸡菊的花蕾及花瓣中Pb、Ni、Hg、Cd、As含量均对人体无健康风险。结论本研究明确了两色金鸡菊花多种元素的积累分布,为新疆两色金鸡菊质量标准全面评估提供了理论依据。
