Heteroclitin D （H.D） was successfully isolated from Kadsurae Caulis by using flash chromatography and recrystalllzed by methanol, 10.2 mg of H.D was obtained from 4.86 g of crude extract, and the purity determined b...Heteroclitin D （H.D） was successfully isolated from Kadsurae Caulis by using flash chromatography and recrystalllzed by methanol, 10.2 mg of H.D was obtained from 4.86 g of crude extract, and the purity determined by HPLC was 99.4%. The structure was identified by UV, IR, MS, and NMR analysis. The fast, simple and efficient method can be applied to the preparation of reference substance of H. D.展开更多
Simultaneous quantification of Lamivudine and Zidovudine in tablets by HPTLC method was developed and validated.The chromatograms were developed using a mobile phase of toluene:ethyl acetate:methanol (4:4:2,v/v/v) on ...Simultaneous quantification of Lamivudine and Zidovudine in tablets by HPTLC method was developed and validated.The chromatograms were developed using a mobile phase of toluene:ethyl acetate:methanol (4:4:2,v/v/v) on pre-coated plate of silica gel GF aluminum TLC plate and quantified by densitometric absorbance mode at 276 nm.The R f values were 0.4170.03 and 0.6070.04 for Lamivudine and Zidovudine,respectively.The linearity of the method was found to be within the concentration range of 50 250 ng/spot for Lamivudine and for Zidovudine,it was 100 500 ng/spot.The lower limits of detection and quantification were 2.23 ng/spot and 7.90 ng/spot for Lamivudine and 2.90 ng/spot and 8.85 ng/spot for Zidovudine.The method was also validated for precision,specificity and recovery.This developed method was used to analyze fixed-dose tablets (Duovir,Cipla Ltd) samples of Lamivudine and Zidovudine.展开更多
The separation of enantiomers of a series of eighteen novel nitrogen mustard linked phosphoryl diamide derivatives was investigated on the prepared phenyl carbamate derivative β-cy-clodextrin bonded phase in normal-p...The separation of enantiomers of a series of eighteen novel nitrogen mustard linked phosphoryl diamide derivatives was investigated on the prepared phenyl carbamate derivative β-cy-clodextrin bonded phase in normal-phase HPLC. Some of the enantiomers could be separated in baseline. The chiral recognition mechanism was also suggested for the separation of chiral phosphorus organic compounds.展开更多
基金supported by the International Scientific and Technological Cooperation Program of China(No:2009DFA31230)the Industry-University-Research Cooperation Program from Science and Technology Department of Qingyuang City(No:2012D021211001)
文摘Heteroclitin D （H.D） was successfully isolated from Kadsurae Caulis by using flash chromatography and recrystalllzed by methanol, 10.2 mg of H.D was obtained from 4.86 g of crude extract, and the purity determined by HPLC was 99.4%. The structure was identified by UV, IR, MS, and NMR analysis. The fast, simple and efficient method can be applied to the preparation of reference substance of H. D.
文摘Simultaneous quantification of Lamivudine and Zidovudine in tablets by HPTLC method was developed and validated.The chromatograms were developed using a mobile phase of toluene:ethyl acetate:methanol (4:4:2,v/v/v) on pre-coated plate of silica gel GF aluminum TLC plate and quantified by densitometric absorbance mode at 276 nm.The R f values were 0.4170.03 and 0.6070.04 for Lamivudine and Zidovudine,respectively.The linearity of the method was found to be within the concentration range of 50 250 ng/spot for Lamivudine and for Zidovudine,it was 100 500 ng/spot.The lower limits of detection and quantification were 2.23 ng/spot and 7.90 ng/spot for Lamivudine and 2.90 ng/spot and 8.85 ng/spot for Zidovudine.The method was also validated for precision,specificity and recovery.This developed method was used to analyze fixed-dose tablets (Duovir,Cipla Ltd) samples of Lamivudine and Zidovudine.
基金Project supported by the Fundation of National Laboratory of Elemento-Organic Chemistry, Nankai University.
文摘The separation of enantiomers of a series of eighteen novel nitrogen mustard linked phosphoryl diamide derivatives was investigated on the prepared phenyl carbamate derivative β-cy-clodextrin bonded phase in normal-phase HPLC. Some of the enantiomers could be separated in baseline. The chiral recognition mechanism was also suggested for the separation of chiral phosphorus organic compounds.
