AIM： To investigate the role of major non-protein and protein sulfhydryls and disulfides in chemically induced gastric hemorrhagic mucosal lesions （HML） and the mechanism of gastroprotective effect of sucralfate.ME...AIM： To investigate the role of major non-protein and protein sulfhydryls and disulfides in chemically induced gastric hemorrhagic mucosal lesions （HML） and the mechanism of gastroprotective effect of sucralfate.METHODS： Rats were given 1 mL of 75% ethanol, 25%NaCl, 0.6 mol/L HCI, 0.2 mol/L NaOH or 1% ammonia solutions intragastrically （i.g.） and sacrificed 1, 3, 6 or 12 min later. Total （reduced and oxidized） glutathione （GSH ＋ GSSG）, glutathione disulfide （GSSG）, protein free sulfhydryls （PSH）, protein-glutathione mixed disulfides （PSSG） and protein cystine disulfides （PSSP） were measured in gastric mucosa and liver.RESULTS： Reduced glutathione （GSH） was depleted in the gastric mucosa after ethanol, HCI or NaCl exposure,while oxidized glutathione （GSSG） concentrations increased, except by HCI and NaOH exposure. Decreased levels of PSH after exposure to ethanol were observed,NaCl or NaOH while the total protein disulfides were increased. Ratios of reduced to oxidized glutathione or sulfhydrils to disulfides were decreased by all chemicals.No changes in thiol homeostasis were detected in the liver after i.g. abbreviation should be spelled out the first time here administration of ethanol. Sucralfate increased the concentrations of GSH and PSH and prevented the ethanol-induced changes in gastric mucosal thiol concentrations.CONCLUSION： Our modified methods are now suitable for direct measurements of major protein and nonprotein thiols/disulfides in the gastric mucosa or liver.A common element in the pathogenesis of chemically induced HML and in the mechanism of gastroprotective drugs seems to be the decreased ratios of reduced and oxidized glutathione as well as protein sulfhydryls and disulfides.展开更多
[Objective] The experiment was conducted to investigate the growth performance of graze cattle fed on non-protein nitrogen （NPN） supplement. [ Method] The experiment was conducted in late spring and early summer bef...[Objective] The experiment was conducted to investigate the growth performance of graze cattle fed on non-protein nitrogen （NPN） supplement. [ Method] The experiment was conducted in late spring and early summer before grazing. Thirty Chinese SimmentaI-Taihang taxa bulls at the age of 9-10 months weighing 150-170 kg were divided into the control group, experimental group 1 and experimental group 2, 10 in each group, Each cattle in the experimental group 1 fed on the basal diet supplemented 1.0 kg cornmeal every day. Each cattle in the experimental group 2 fed on the basal diet supplemented 1.0 kg cornmeal and 0.25 kg NPN supplement every day. Those in the control group were fed on the basal di- et. The experiment was performed for 100 d. [Result] The average daily gain of the experimental group I was 217 g larger than that of the control group, while the average daily gain of the experimental group 2 was 429 g larger than that of the control group. The differences in the average daily gain were significant between the three groups. [ Conclusion ] The growth performance of cattle grazed in northern slopes was obviously improved by supplementing a small amount of concentrate in feed. The improvement was more remarkable if a small amount of NPN supplement was also added in feed.展开更多
Slow-release non-protein nitrogen feed has a large market demand. It has a long research history, but its production technology needs further improvement in order to realize the industrial production of slow-release n...Slow-release non-protein nitrogen feed has a large market demand. It has a long research history, but its production technology needs further improvement in order to realize the industrial production of slow-release non-protein nitrogen extruded feed. By designing the best formula and using chelating and emulsifying process, the slow-release non-protein nitrogen extruded feed additives were produced. This product increases milk yield and improves milk quality, thus increasing economic efficiency.展开更多
Protein in rice grains is an important source of nutrition for rice consumers.This study mainly aimed to identify the critical factors that determine grain protein concentration in rice.Accumulation parameters,includi...Protein in rice grains is an important source of nutrition for rice consumers.This study mainly aimed to identify the critical factors that determine grain protein concentration in rice.Accumulation parameters,including mean accumulation rate(Rmean)and active accumulation duration(Dactive),for protein and non-protein components and their correlations with protein concentration in rice grains were investigated in field experiments conducted over two years with six rice cultivars.Results showed that grain protein concentration ranged from 9.6%to 11.9%across cultivars and years.Accumulation processes of protein and non-protein components were well fitted by the logistic equation for all six rice cultivars in both years,and the ratio of protein to non-protein for R_(mean) and D_(active) ranged from 0.08 to 0.12 and 1.01 to 1.33,respectively.Grain protein concentration was significantly correlated with protein to non-protein ratio for R_(mean).This study suggests that grain protein concentration is not solely determined by the accumulation of protein or non-protein component,but by the coordination of protein and non-protein accumulation.展开更多
目的:制备急性海马脑片,探讨睾酮(T)、双氢睾酮(DHT)、睾酮-牛血清白蛋白(T-BSA)对海马脑片神经元树突棘密度和突触蛋白[突触素(SYN)、突触后致密物质95(PSD95)]的非基因组效应。方法:选用3月龄SD雄性大鼠,麻醉后取脑,在人工脑脊液中剥...目的:制备急性海马脑片,探讨睾酮(T)、双氢睾酮(DHT)、睾酮-牛血清白蛋白(T-BSA)对海马脑片神经元树突棘密度和突触蛋白[突触素(SYN)、突触后致密物质95(PSD95)]的非基因组效应。方法:选用3月龄SD雄性大鼠,麻醉后取脑,在人工脑脊液中剥离海马组织,制备300μm的冠状海马脑片。随机在0、15、30、60、120 min 5个时间点使用细胞核染料DAPI和PI染色,评估神经元活性。将急性海马脑片随机分为对照组、睾酮组、DHT组和T-BSA组。睾酮和DHT的药物作用浓度为10 nmol/L,T-BSA为0.36 nmol/L。给药1 h后,通过Golgi-Cox染色、免疫荧光组织化学染色和免疫印迹观察各组神经元树突棘密度以及SYN和PSD95的变化。结果:DAPI和PI同时染色显示,急性海马脑片神经元在0、15、30、60、120 min 5个时间点上活细胞百分比差异无统计学意义。Golgi-Cox染色实验显示,与对照组相比,睾酮组、DHT组和T-BSA组的树突棘密度明显增加;睾酮组、DHT组和T-BSA组之间树突棘密度差异无统计学意义。免疫荧光组织化学染色和免疫印迹实验显示,与对照组相比,睾酮组、DHT组和T-BSA组的SYN和PSD95蛋白表达明显增加;睾酮组、DHT组和T-BSA组之间SYN和PSD95蛋白表达差异无统计学意义。结论:制备的急性海马脑片神经元在2 h内细胞活性良好。雄激素通过非基因组效应增加了急性海马脑片神经元树突棘密度以及SYN和PSD95的表达。展开更多
The complex responses of eukaryotic cells to external factors are governed by several transcriptional and post-transcriptional processes. Several of them occur in the nucleus and have been linked to the action of non-...The complex responses of eukaryotic cells to external factors are governed by several transcriptional and post-transcriptional processes. Several of them occur in the nucleus and have been linked to the action of non-proteincoding RNAs （or npcRNAs）, both long and small npcRNAs, that recently emerged as major regulators of gene expression. Regulatory npcRNAs acting in the nucleus include silencing-related RNAs, intergenic npcRNAs, natural antisense RNAs, and other aberrant RNAs resulting from the interplay between global transcription and RNA processing activities （such as Dicers and RNA-dependent polymerases）. Generally, the resulting npcRNAs exert their regulatory effects through interactions with RNA-binding proteins （or RBPs） within ribonucleoprotein particles （or RNPs）. A large group of RBPs are implicated in the silencing machinery through small interfering RNAs （siRNAs） and their localization suggests that several act in the nucleus to trigger epigenetic and chromatin changes at a whole-genome scale. Other nuclear RBPs interact with npcRNAs and change their localization. In the fission yeast, the RNA-binding Mei2p protein, playing pivotal roles in meiosis, interact with a meiotic npcRNA involved in its nuclear re-localization. Related processes have been identified in plants and the ENOD40 npcRNA was shown to re-localize a nuclear-speckle RBP from the nucleus to the cytoplasm in Medicago truncatula. Plant RBPs have been also implicated in RNA-mediated chromatin silencing in the FLC locus through interaction with specific antisense transcripts. In this review, we discuss the interactions between RBPs and npcRNAs in the context of nuclear-related processes and their implication in plant development and stress responses. We propose that these interactions may add a regulatory layer that modulates the interactions between the nuclear genome and the environment and, consequently, control plant developmental plasticity.展开更多
文摘AIM： To investigate the role of major non-protein and protein sulfhydryls and disulfides in chemically induced gastric hemorrhagic mucosal lesions （HML） and the mechanism of gastroprotective effect of sucralfate.METHODS： Rats were given 1 mL of 75% ethanol, 25%NaCl, 0.6 mol/L HCI, 0.2 mol/L NaOH or 1% ammonia solutions intragastrically （i.g.） and sacrificed 1, 3, 6 or 12 min later. Total （reduced and oxidized） glutathione （GSH ＋ GSSG）, glutathione disulfide （GSSG）, protein free sulfhydryls （PSH）, protein-glutathione mixed disulfides （PSSG） and protein cystine disulfides （PSSP） were measured in gastric mucosa and liver.RESULTS： Reduced glutathione （GSH） was depleted in the gastric mucosa after ethanol, HCI or NaCl exposure,while oxidized glutathione （GSSG） concentrations increased, except by HCI and NaOH exposure. Decreased levels of PSH after exposure to ethanol were observed,NaCl or NaOH while the total protein disulfides were increased. Ratios of reduced to oxidized glutathione or sulfhydrils to disulfides were decreased by all chemicals.No changes in thiol homeostasis were detected in the liver after i.g. abbreviation should be spelled out the first time here administration of ethanol. Sucralfate increased the concentrations of GSH and PSH and prevented the ethanol-induced changes in gastric mucosal thiol concentrations.CONCLUSION： Our modified methods are now suitable for direct measurements of major protein and nonprotein thiols/disulfides in the gastric mucosa or liver.A common element in the pathogenesis of chemically induced HML and in the mechanism of gastroprotective drugs seems to be the decreased ratios of reduced and oxidized glutathione as well as protein sulfhydryls and disulfides.
基金funded by the National Key Technology R&D Program for the 11th Five-Year Plan (2006BAD29B05 and 2006BAD56B04)Achievements Conversion Fund of Shanxi Science and Technology Department,Animal Breeding Fund of Shanxi Academy of Agricultural Sciences,Shanxi Natural Science Foundation (2010011040-4)Doctor Research Fund of Shanxi Academy of Agricultural Sciences (YBSJJ0901)
文摘[Objective] The experiment was conducted to investigate the growth performance of graze cattle fed on non-protein nitrogen （NPN） supplement. [ Method] The experiment was conducted in late spring and early summer before grazing. Thirty Chinese SimmentaI-Taihang taxa bulls at the age of 9-10 months weighing 150-170 kg were divided into the control group, experimental group 1 and experimental group 2, 10 in each group, Each cattle in the experimental group 1 fed on the basal diet supplemented 1.0 kg cornmeal every day. Each cattle in the experimental group 2 fed on the basal diet supplemented 1.0 kg cornmeal and 0.25 kg NPN supplement every day. Those in the control group were fed on the basal di- et. The experiment was performed for 100 d. [Result] The average daily gain of the experimental group I was 217 g larger than that of the control group, while the average daily gain of the experimental group 2 was 429 g larger than that of the control group. The differences in the average daily gain were significant between the three groups. [ Conclusion ] The growth performance of cattle grazed in northern slopes was obviously improved by supplementing a small amount of concentrate in feed. The improvement was more remarkable if a small amount of NPN supplement was also added in feed.
基金funded by the Higher Vocational Colleges and Higher Junior Colleges Research Project of Heilongjiang Provincial Education Department (11515077)
文摘Slow-release non-protein nitrogen feed has a large market demand. It has a long research history, but its production technology needs further improvement in order to realize the industrial production of slow-release non-protein nitrogen extruded feed. By designing the best formula and using chelating and emulsifying process, the slow-release non-protein nitrogen extruded feed additives were produced. This product increases milk yield and improves milk quality, thus increasing economic efficiency.
基金supported by the National Key R&D Program of China(2016YFD0300509).
文摘Protein in rice grains is an important source of nutrition for rice consumers.This study mainly aimed to identify the critical factors that determine grain protein concentration in rice.Accumulation parameters,including mean accumulation rate(Rmean)and active accumulation duration(Dactive),for protein and non-protein components and their correlations with protein concentration in rice grains were investigated in field experiments conducted over two years with six rice cultivars.Results showed that grain protein concentration ranged from 9.6%to 11.9%across cultivars and years.Accumulation processes of protein and non-protein components were well fitted by the logistic equation for all six rice cultivars in both years,and the ratio of protein to non-protein for R_(mean) and D_(active) ranged from 0.08 to 0.12 and 1.01 to 1.33,respectively.Grain protein concentration was significantly correlated with protein to non-protein ratio for R_(mean).This study suggests that grain protein concentration is not solely determined by the accumulation of protein or non-protein component,but by the coordination of protein and non-protein accumulation.
文摘目的:制备急性海马脑片,探讨睾酮(T)、双氢睾酮(DHT)、睾酮-牛血清白蛋白(T-BSA)对海马脑片神经元树突棘密度和突触蛋白[突触素(SYN)、突触后致密物质95(PSD95)]的非基因组效应。方法:选用3月龄SD雄性大鼠,麻醉后取脑,在人工脑脊液中剥离海马组织,制备300μm的冠状海马脑片。随机在0、15、30、60、120 min 5个时间点使用细胞核染料DAPI和PI染色,评估神经元活性。将急性海马脑片随机分为对照组、睾酮组、DHT组和T-BSA组。睾酮和DHT的药物作用浓度为10 nmol/L,T-BSA为0.36 nmol/L。给药1 h后,通过Golgi-Cox染色、免疫荧光组织化学染色和免疫印迹观察各组神经元树突棘密度以及SYN和PSD95的变化。结果:DAPI和PI同时染色显示,急性海马脑片神经元在0、15、30、60、120 min 5个时间点上活细胞百分比差异无统计学意义。Golgi-Cox染色实验显示,与对照组相比,睾酮组、DHT组和T-BSA组的树突棘密度明显增加;睾酮组、DHT组和T-BSA组之间树突棘密度差异无统计学意义。免疫荧光组织化学染色和免疫印迹实验显示,与对照组相比,睾酮组、DHT组和T-BSA组的SYN和PSD95蛋白表达明显增加;睾酮组、DHT组和T-BSA组之间SYN和PSD95蛋白表达差异无统计学意义。结论:制备的急性海马脑片神经元在2 h内细胞活性良好。雄激素通过非基因组效应增加了急性海马脑片神经元树突棘密度以及SYN和PSD95的表达。
文摘The complex responses of eukaryotic cells to external factors are governed by several transcriptional and post-transcriptional processes. Several of them occur in the nucleus and have been linked to the action of non-proteincoding RNAs （or npcRNAs）, both long and small npcRNAs, that recently emerged as major regulators of gene expression. Regulatory npcRNAs acting in the nucleus include silencing-related RNAs, intergenic npcRNAs, natural antisense RNAs, and other aberrant RNAs resulting from the interplay between global transcription and RNA processing activities （such as Dicers and RNA-dependent polymerases）. Generally, the resulting npcRNAs exert their regulatory effects through interactions with RNA-binding proteins （or RBPs） within ribonucleoprotein particles （or RNPs）. A large group of RBPs are implicated in the silencing machinery through small interfering RNAs （siRNAs） and their localization suggests that several act in the nucleus to trigger epigenetic and chromatin changes at a whole-genome scale. Other nuclear RBPs interact with npcRNAs and change their localization. In the fission yeast, the RNA-binding Mei2p protein, playing pivotal roles in meiosis, interact with a meiotic npcRNA involved in its nuclear re-localization. Related processes have been identified in plants and the ENOD40 npcRNA was shown to re-localize a nuclear-speckle RBP from the nucleus to the cytoplasm in Medicago truncatula. Plant RBPs have been also implicated in RNA-mediated chromatin silencing in the FLC locus through interaction with specific antisense transcripts. In this review, we discuss the interactions between RBPs and npcRNAs in the context of nuclear-related processes and their implication in plant development and stress responses. We propose that these interactions may add a regulatory layer that modulates the interactions between the nuclear genome and the environment and, consequently, control plant developmental plasticity.
