A novel element at -153/-143 bp in the inter-leukin 2 receptor α(IL-2Rα) gene has been coined as NRE-inverse repeat sequence (NIRS) due to its inversely repeated to the known negative regulatory element (NRE) furthe...A novel element at -153/-143 bp in the inter-leukin 2 receptor α(IL-2Rα) gene has been coined as NRE-inverse repeat sequence (NIRS) due to its inversely repeated to the known negative regulatory element (NRE) further upstream of the gene. In order to explore the role of NIRS in the expression of IL-2Rα gene, luciferase reporter plasmids driven by 4 individually deleted IL-2Rα genes promoter regions were constructed. Transfection of the reporter plasmids into Jurkat cells and HeLa cells respectively, we found that both NIRS and NRE were critical for repressing the constitutive expression of IL-2Rα gene and were also necessary for promoter activity induced by PHA. EMSA results showed that double-stranded NRE- and NIRS-bind-ing proteins existed in both HeLa cells and Jurkat cells. However, single-stranded NIRS- and NRE-binding protein was only found in HeLa cells. Interestingly, the supershift band showed up in EMSA system with Jurkat cells (no matter whether activated or not) adding to the cell展开更多
Two numerical experiments were performed by using ECMWF limited area model in order to assess the impact of radiative processes on the onset of summer monsoon.The control experiment includes all model physics and the ...Two numerical experiments were performed by using ECMWF limited area model in order to assess the impact of radiative processes on the onset of summer monsoon.The control experiment includes all model physics and the other experiment is exclusive of radiative processes.The simulated results show that the radiative processes enhanced the monsoon circulation and the associated vertical circulation over South Asia.Especially over the Qinghai-Xizang Plateau,the build-up of the plateau monsoon depression and the beginning of the plat- eau rainy season are highly dependent upon the radiative processes. Through the radiation-cloud interaction,the “moist processes” were enhanced over land.More latent heat was released.The feedback of the physical processes caused the strengthening of the monsoon circulation over southern Asia.展开更多
基金This work was supported by theNational Natural Science Foundation of China (Grant Nos. 39480007 and 39670168).
文摘A novel element at -153/-143 bp in the inter-leukin 2 receptor α(IL-2Rα) gene has been coined as NRE-inverse repeat sequence (NIRS) due to its inversely repeated to the known negative regulatory element (NRE) further upstream of the gene. In order to explore the role of NIRS in the expression of IL-2Rα gene, luciferase reporter plasmids driven by 4 individually deleted IL-2Rα genes promoter regions were constructed. Transfection of the reporter plasmids into Jurkat cells and HeLa cells respectively, we found that both NIRS and NRE were critical for repressing the constitutive expression of IL-2Rα gene and were also necessary for promoter activity induced by PHA. EMSA results showed that double-stranded NRE- and NIRS-bind-ing proteins existed in both HeLa cells and Jurkat cells. However, single-stranded NIRS- and NRE-binding protein was only found in HeLa cells. Interestingly, the supershift band showed up in EMSA system with Jurkat cells (no matter whether activated or not) adding to the cell
文摘Two numerical experiments were performed by using ECMWF limited area model in order to assess the impact of radiative processes on the onset of summer monsoon.The control experiment includes all model physics and the other experiment is exclusive of radiative processes.The simulated results show that the radiative processes enhanced the monsoon circulation and the associated vertical circulation over South Asia.Especially over the Qinghai-Xizang Plateau,the build-up of the plateau monsoon depression and the beginning of the plat- eau rainy season are highly dependent upon the radiative processes. Through the radiation-cloud interaction,the “moist processes” were enhanced over land.More latent heat was released.The feedback of the physical processes caused the strengthening of the monsoon circulation over southern Asia.
