Sea cucumber（Apostichopus japonicus） is one of the economically important farmed echinoderm species in North China. The NF-κB/Rel transcription factors can function as key regulators to modulate the expression of i...Sea cucumber（Apostichopus japonicus） is one of the economically important farmed echinoderm species in North China. The NF-κB/Rel transcription factors can function as key regulators to modulate the expression of immune-related genes in response to immune challenge or environmental stress. And as a crucial enzyme in innate immunity, lysozyme plays a key role in the overall defense against pathogens in A. japonicus. In the present study, the expression of NF-κB/Rel and lysozyme mRNA against bacterial challenge in sea cucumber was investigated. After bacterial challenge, the Aj-rel mRNA transcripts increased significantly and reached the peak at 10 min（3.26-fold, P0.05）; the Aj-p105 gene expression showed a rising trend and reached the peak at 240 min（6.12-fold, P0.05）; and the Aj-p50 mRNA transcripts showed a downward trend and reached the peak at 10 min（17.80-fold, P 0.05）. The lysozyme mRNA expression level showed two peaks at 10 min（1.54-fold, P 0.05） and 240 min（2.48-fold, P 0.05）, respectively. This study will provide certain reference for further understanding on the immune defense mechanism and offer enlightenment to disease control of echinoderm.展开更多
基金Supported by Key Technologies R&D Program of Shandong Province(2014GHY115024)Presidential Foundation of Marine Biology Institute of Shandong Province(SZJJ201303)~~
文摘Sea cucumber（Apostichopus japonicus） is one of the economically important farmed echinoderm species in North China. The NF-κB/Rel transcription factors can function as key regulators to modulate the expression of immune-related genes in response to immune challenge or environmental stress. And as a crucial enzyme in innate immunity, lysozyme plays a key role in the overall defense against pathogens in A. japonicus. In the present study, the expression of NF-κB/Rel and lysozyme mRNA against bacterial challenge in sea cucumber was investigated. After bacterial challenge, the Aj-rel mRNA transcripts increased significantly and reached the peak at 10 min（3.26-fold, P0.05）; the Aj-p105 gene expression showed a rising trend and reached the peak at 240 min（6.12-fold, P0.05）; and the Aj-p50 mRNA transcripts showed a downward trend and reached the peak at 10 min（17.80-fold, P 0.05）. The lysozyme mRNA expression level showed two peaks at 10 min（1.54-fold, P 0.05） and 240 min（2.48-fold, P 0.05）, respectively. This study will provide certain reference for further understanding on the immune defense mechanism and offer enlightenment to disease control of echinoderm.
