Experiments were carried out on a vegetable field with Peking cabbage(Brassica pekinensis (Lour.) Rupr.), cabbage (Brassica, chinensis var. oleifcra Makino and nemoto),green cabbage (Brassica chinensis L.), spinach (S...Experiments were carried out on a vegetable field with Peking cabbage(Brassica pekinensis (Lour.) Rupr.), cabbage (Brassica, chinensis var. oleifcra Makino and nemoto),green cabbage (Brassica chinensis L.), spinach (Spinacia oleracea L.) and rape(Brassica campestrisL.) to study the effects of N forms and N rates on their growth and nitrate accumulation. Theresults indicated that application of ammonium chloride, ammonium nitrate, sodium nitrate and ureasignificantly increased the yields and nitrate concentrations of Peking cabbage and spinach.Although no significant difference was found in the yields after application of the 4 N forms,nitrate N increased nitrate accumulation in vegetables much more than ammonium N. The vegetableyields were not increased continuously with N rate increase, and oversupply of N reduced the plantgrowth, leading to a yield decline. This trend was also true for nitrate concentrations in somevegetables and at some sampling times. However, as a whole, the nitrate concentrations in vegetableswere positively correlated with N rates. Thus, addition of N fertilizer to soil was the major causefor increases in nitrate concentrations in vegetables. Nitrate concentrations were much higher inroots, stems and petioles than in blades at any N rate.展开更多
Changes of soil organic nitrogen forms and soil nitrogen supply under continuous rice cropping system were investigated in a long-term fertilization experiment in Jinhua, Zhejiang Province, China. The fertilizer treat...Changes of soil organic nitrogen forms and soil nitrogen supply under continuous rice cropping system were investigated in a long-term fertilization experiment in Jinhua, Zhejiang Province, China. The fertilizer treatments included combination of P-K, N-K, N-P, and N-P-K as well as the control. After six years of continuous double-rice cropping, total soil N and hydrolysable N contents remained stable in plots with N treatments, while the hydrolysable N contents were substantially reduced in those plots without N application. Compared to the unbalanced fertilization treatments, P and K increased the percentage of hydrolysable ammonium N in the total soil N with the balanced application of N, and also maintained higher rice grain yields and nitrogen uptake. Grain yield was positively correlated with total N uptake (r = 0.875**), hydrolysable N (r = 0.608**), hydrolysable ammonium N (r = 0.560**) and the hydrolysable unknown N (r = 0.417**). Total N uptake was positively correlated with hydrolysable N (r = 0.608**), hydrolysable ammonium N (r = 0.440**) and hydrolysable unknown N (r = 0.431**). Soil nutrient depletion and/or unbalanced fertilization to rice crop reduced N content in soil microbial biomass, and therefore increased C/N ratio, suggesting a negative effect on the total microbial biomass in the soil.展开更多
The growth, chlorophyll composition, photosynthesis, respiration, K uptake and root K+ secretion offlue-cured tobacco as thected by different concentrations of N supplied as urea, NaNO3 and NH4NO3 werestudied under th...The growth, chlorophyll composition, photosynthesis, respiration, K uptake and root K+ secretion offlue-cured tobacco as thected by different concentrations of N supplied as urea, NaNO3 and NH4NO3 werestudied under the experimental condition of sand culture. The results showed that the content of K in thefiue-cured tobacco was not merely related with root vitality and uptake but also closely related with root cellmembrane structure and K+ secretion.展开更多
Effects of different levels of compost application on the amounts and percentage distribution of organic N forms in whole soils and particle size fractions were investigated. Soil samples were collected from three plo...Effects of different levels of compost application on the amounts and percentage distribution of organic N forms in whole soils and particle size fractions were investigated. Soil samples were collected from three plots: (a) F, only chemical fertilizers;(b) F+LC, chemical fertilizers plus low level of compost;(c) F+HC, chemical fertilizers plus high level of compost. Each soil sample was divided into five fractions: coarse sand-sized aggregate (CSA), medium sand-sized aggregate (MSA), fine sand-sized aggregate (FSA), silt-sized aggregate (SIA) and clay-sized aggregate (CLA) fractions. The sand fractions were subdivided into decayed plants (DP) and mineral particles (MP). The amounts of total N and different organic N forms in the whole soils as well as size fractions generally increased with increasing the amount of compost. In the whole soils, percentage distribution of non-hydrolysable-N and amino sugar-N increased by compost application while the distribution values of the hydrolysable ammonium- N and unidentified-N decreased. The application did not affect the distribution degree of amino acid-N. In the size fractions, the distribution values of most organic N forms increased in the CSA-DP, MSA-DP and FSA-DP fractions by compost application. In the CLA fractions, the amounts and percentage distribution of organic N forms were the highest, although the application caused decreases in their distribution values. These findings indicate that the CLA fraction merit close attention as an important reservoir of various organic N.展开更多
Nitrogen forms of humic substances from a subalpine meadow soil, a latentic red soil and a weathered coal and the effect of acid hydrolysis on N structures of soil humic substances were studied by using 15N cross-pola...Nitrogen forms of humic substances from a subalpine meadow soil, a latentic red soil and a weathered coal and the effect of acid hydrolysis on N structures of soil humic substances were studied by using 15N cross-polarization magic angle spinning nuclear magnetic resonance (CPMAS NMR) spectroscopy. Of the detectable 15N-signal intensity in the spectra of soil humic substances 71%-79% may be attributed to amide groups, 10%-18% to aromatic/aliphatic amines and 6%~11% to indole- and pyrrole-like N. Whereas in the spectrum of the fulvic acid from weathered coal 46%, at least, of the total 15N-signal intensity might be assigned to pyrrole-like N, 14% to aromatic/aliphatic ammes, and the remaining intensities could not be assigned with certainty. Data on nonhydrolyzable residue of protein-sugar mixture and a 15N-labelled soil fulvic acid confirm the formation of nonhydrolyzable heterocyclic N during acid hydrolysis. Project (No. 39790100) supported by the National Natural Science Foundation of China.展开更多
Litter decomposition is the fundamental process in nutrient cycling and soil carbon（C） sequestration in terrestrial ecosystems. The global-wide increase in nitrogen（N） inputs is expected to alter litter decomposit...Litter decomposition is the fundamental process in nutrient cycling and soil carbon（C） sequestration in terrestrial ecosystems. The global-wide increase in nitrogen（N） inputs is expected to alter litter decomposition and,ultimately, affect ecosystem C storage and nutrient status. Temperate grassland ecosystems in China are usually N-deficient and particularly sensitive to the changes in exogenous N additions. In this paper, we conducted a 1,200-day in situ experiment in a typical semi-arid temperate steppe in Inner Mongolia to investigate the litter decomposition as well as the dynamics of litter C and N concentrations under three N addition levels（low N with 50 kg N/（hm2？a）（LN）, medium N with 100 kg N/（hm2？a）（MN）, and high N with 200 kg N/（hm2？a）（HN）） and three N addition forms（ammonium-N-based with 100 kg N/（hm2？a） as ammonium sulfate（AS）, nitrate-N-based with 100 kg N/（hm2？a） as sodium nitrate（SN）, and mixed-N-based with 100 kg N/（hm2？a） as calcium ammonium nitrate（CAN）） compared to control with no N addition（CK）. The results indicated that the litter mass remaining in all N treatments exhibited a similar decomposition pattern： fast decomposition within the initial 120 days, followed by a relatively slow decomposition in the remaining observation period（120–1,200 days）. The decomposition pattern in each treatment was fitted well in two split-phase models, namely, a single exponential decay model in phase I（〈398 days） and a linear decay function in phase II（≥398 days）. The three N addition levels exerted insignificant effects on litter decomposition in the early stages（〈398 days, phase I; P〉0.05）. However, MN and HN treatments inhibited litter mass loss after 398 and 746 days, respectively（P〈0.05）. AS and SN treatments exerted similar effects on litter mass remaining during the entire decomposition period（P〉0.05）. The effects of these two N addition forms differed greatly from those of CAN after 746 and 1,053 days, respectively（P〈0.05）. During the decomposition period, N concentrations in the decomposing litter increased whereas C concentrations decreased, which also led to an exponential decrease in litter C：N ratios in all treatments. No significant effects were induced by N addition levels and forms on litter C and N concentrations（P〉0.05）. Our results indicated that exogenous N additions could exhibit neutral or inhibitory effects on litter decomposition, and the inhibitory effects of N additions on litter decomposition in the final decay stages are not caused by the changes in the chemical qualities of the litter, such as endogenous N and C concentrations. These results will provide an important data basis for the simulation and prediction of C cycle processes in future N-deposition scenarios.展开更多
Five humic fractions were obtained from a uniformly 15N-labelled soil by extraction with 0.1 mol L-1 Na4P207, 0.1 mol L-1 NaOH, and HF/HCI-0.1 mol L-1 NaOH, consecutively, and analyzed by 13C and 15N CPMAS NMR (cross ...Five humic fractions were obtained from a uniformly 15N-labelled soil by extraction with 0.1 mol L-1 Na4P207, 0.1 mol L-1 NaOH, and HF/HCI-0.1 mol L-1 NaOH, consecutively, and analyzed by 13C and 15N CPMAS NMR (cross polarization and magic angle spinning nuclear magnetic resonance). Compared with those of native soils humic fractions studied as a whole contained more alkyls, methoxyls and O-alkyls, being 27%-36%, 17%-21% and 36%-40%, respectively, but fewer aromatics and carboxyls (being 14%-20% and 13%-90%, respectively). Among those humic fractions, the humic acid (HA) and fulvic acid (FA) extracted by 0.1 mol L-1 Na4P207 contained slightly more carboxyls than corresponding humic fractions extracted by 0.1 mol L-1 NaOH, and the HA extracted by 0.1 mol L-1 NaOH after treatment with HF/HCI contained the least aromatics and carboxyls. The distribution of nitrogen functional groups of soil humic fractions studied was quite similar to each other and also quite similar to that of humic fraction from native soils. More than 75% of total N in each fraction was in amide form, with 9%-13% present as aromatic and/or aliphatic amines and the remainder as hoterocyclic N.展开更多
基金Project supported by the National Key Basic Research Support Foundation (No. G1999011707)the National Natural Science Foundation of China (Nos. 39970429, 49890330, 30070429 and 40201028).
文摘Experiments were carried out on a vegetable field with Peking cabbage(Brassica pekinensis (Lour.) Rupr.), cabbage (Brassica, chinensis var. oleifcra Makino and nemoto),green cabbage (Brassica chinensis L.), spinach (Spinacia oleracea L.) and rape(Brassica campestrisL.) to study the effects of N forms and N rates on their growth and nitrate accumulation. Theresults indicated that application of ammonium chloride, ammonium nitrate, sodium nitrate and ureasignificantly increased the yields and nitrate concentrations of Peking cabbage and spinach.Although no significant difference was found in the yields after application of the 4 N forms,nitrate N increased nitrate accumulation in vegetables much more than ammonium N. The vegetableyields were not increased continuously with N rate increase, and oversupply of N reduced the plantgrowth, leading to a yield decline. This trend was also true for nitrate concentrations in somevegetables and at some sampling times. However, as a whole, the nitrate concentrations in vegetableswere positively correlated with N rates. Thus, addition of N fertilizer to soil was the major causefor increases in nitrate concentrations in vegetables. Nitrate concentrations were much higher inroots, stems and petioles than in blades at any N rate.
基金Project supported by the International Fertilizer Industry Association (IFI), Francethe Potast & Phosphate Institute (PPI), USA and Canadathe International Potassium Institute (IPI), Switzerland
文摘Changes of soil organic nitrogen forms and soil nitrogen supply under continuous rice cropping system were investigated in a long-term fertilization experiment in Jinhua, Zhejiang Province, China. The fertilizer treatments included combination of P-K, N-K, N-P, and N-P-K as well as the control. After six years of continuous double-rice cropping, total soil N and hydrolysable N contents remained stable in plots with N treatments, while the hydrolysable N contents were substantially reduced in those plots without N application. Compared to the unbalanced fertilization treatments, P and K increased the percentage of hydrolysable ammonium N in the total soil N with the balanced application of N, and also maintained higher rice grain yields and nitrogen uptake. Grain yield was positively correlated with total N uptake (r = 0.875**), hydrolysable N (r = 0.608**), hydrolysable ammonium N (r = 0.560**) and the hydrolysable unknown N (r = 0.417**). Total N uptake was positively correlated with hydrolysable N (r = 0.608**), hydrolysable ammonium N (r = 0.440**) and hydrolysable unknown N (r = 0.431**). Soil nutrient depletion and/or unbalanced fertilization to rice crop reduced N content in soil microbial biomass, and therefore increased C/N ratio, suggesting a negative effect on the total microbial biomass in the soil.
文摘The growth, chlorophyll composition, photosynthesis, respiration, K uptake and root K+ secretion offlue-cured tobacco as thected by different concentrations of N supplied as urea, NaNO3 and NH4NO3 werestudied under the experimental condition of sand culture. The results showed that the content of K in thefiue-cured tobacco was not merely related with root vitality and uptake but also closely related with root cellmembrane structure and K+ secretion.
文摘Effects of different levels of compost application on the amounts and percentage distribution of organic N forms in whole soils and particle size fractions were investigated. Soil samples were collected from three plots: (a) F, only chemical fertilizers;(b) F+LC, chemical fertilizers plus low level of compost;(c) F+HC, chemical fertilizers plus high level of compost. Each soil sample was divided into five fractions: coarse sand-sized aggregate (CSA), medium sand-sized aggregate (MSA), fine sand-sized aggregate (FSA), silt-sized aggregate (SIA) and clay-sized aggregate (CLA) fractions. The sand fractions were subdivided into decayed plants (DP) and mineral particles (MP). The amounts of total N and different organic N forms in the whole soils as well as size fractions generally increased with increasing the amount of compost. In the whole soils, percentage distribution of non-hydrolysable-N and amino sugar-N increased by compost application while the distribution values of the hydrolysable ammonium- N and unidentified-N decreased. The application did not affect the distribution degree of amino acid-N. In the size fractions, the distribution values of most organic N forms increased in the CSA-DP, MSA-DP and FSA-DP fractions by compost application. In the CLA fractions, the amounts and percentage distribution of organic N forms were the highest, although the application caused decreases in their distribution values. These findings indicate that the CLA fraction merit close attention as an important reservoir of various organic N.
基金supported by the National Natural science Foundation of China.(No.39790100)
文摘Nitrogen forms of humic substances from a subalpine meadow soil, a latentic red soil and a weathered coal and the effect of acid hydrolysis on N structures of soil humic substances were studied by using 15N cross-polarization magic angle spinning nuclear magnetic resonance (CPMAS NMR) spectroscopy. Of the detectable 15N-signal intensity in the spectra of soil humic substances 71%-79% may be attributed to amide groups, 10%-18% to aromatic/aliphatic amines and 6%~11% to indole- and pyrrole-like N. Whereas in the spectrum of the fulvic acid from weathered coal 46%, at least, of the total 15N-signal intensity might be assigned to pyrrole-like N, 14% to aromatic/aliphatic ammes, and the remaining intensities could not be assigned with certainty. Data on nonhydrolyzable residue of protein-sugar mixture and a 15N-labelled soil fulvic acid confirm the formation of nonhydrolyzable heterocyclic N during acid hydrolysis. Project (No. 39790100) supported by the National Natural Science Foundation of China.
基金funded by the National Natural Science Foundation of China (41073061, 41203054, 40730105, 40973057)the Knowledge Innovation Program of the Chinese Academy of Sciences (KZCX2-EW-302)
文摘Litter decomposition is the fundamental process in nutrient cycling and soil carbon（C） sequestration in terrestrial ecosystems. The global-wide increase in nitrogen（N） inputs is expected to alter litter decomposition and,ultimately, affect ecosystem C storage and nutrient status. Temperate grassland ecosystems in China are usually N-deficient and particularly sensitive to the changes in exogenous N additions. In this paper, we conducted a 1,200-day in situ experiment in a typical semi-arid temperate steppe in Inner Mongolia to investigate the litter decomposition as well as the dynamics of litter C and N concentrations under three N addition levels（low N with 50 kg N/（hm2？a）（LN）, medium N with 100 kg N/（hm2？a）（MN）, and high N with 200 kg N/（hm2？a）（HN）） and three N addition forms（ammonium-N-based with 100 kg N/（hm2？a） as ammonium sulfate（AS）, nitrate-N-based with 100 kg N/（hm2？a） as sodium nitrate（SN）, and mixed-N-based with 100 kg N/（hm2？a） as calcium ammonium nitrate（CAN）） compared to control with no N addition（CK）. The results indicated that the litter mass remaining in all N treatments exhibited a similar decomposition pattern： fast decomposition within the initial 120 days, followed by a relatively slow decomposition in the remaining observation period（120–1,200 days）. The decomposition pattern in each treatment was fitted well in two split-phase models, namely, a single exponential decay model in phase I（〈398 days） and a linear decay function in phase II（≥398 days）. The three N addition levels exerted insignificant effects on litter decomposition in the early stages（〈398 days, phase I; P〉0.05）. However, MN and HN treatments inhibited litter mass loss after 398 and 746 days, respectively（P〈0.05）. AS and SN treatments exerted similar effects on litter mass remaining during the entire decomposition period（P〉0.05）. The effects of these two N addition forms differed greatly from those of CAN after 746 and 1,053 days, respectively（P〈0.05）. During the decomposition period, N concentrations in the decomposing litter increased whereas C concentrations decreased, which also led to an exponential decrease in litter C：N ratios in all treatments. No significant effects were induced by N addition levels and forms on litter C and N concentrations（P〉0.05）. Our results indicated that exogenous N additions could exhibit neutral or inhibitory effects on litter decomposition, and the inhibitory effects of N additions on litter decomposition in the final decay stages are not caused by the changes in the chemical qualities of the litter, such as endogenous N and C concentrations. These results will provide an important data basis for the simulation and prediction of C cycle processes in future N-deposition scenarios.
基金Project (No. 39790100) supported by the National Natural Science Foundation of China.
文摘Five humic fractions were obtained from a uniformly 15N-labelled soil by extraction with 0.1 mol L-1 Na4P207, 0.1 mol L-1 NaOH, and HF/HCI-0.1 mol L-1 NaOH, consecutively, and analyzed by 13C and 15N CPMAS NMR (cross polarization and magic angle spinning nuclear magnetic resonance). Compared with those of native soils humic fractions studied as a whole contained more alkyls, methoxyls and O-alkyls, being 27%-36%, 17%-21% and 36%-40%, respectively, but fewer aromatics and carboxyls (being 14%-20% and 13%-90%, respectively). Among those humic fractions, the humic acid (HA) and fulvic acid (FA) extracted by 0.1 mol L-1 Na4P207 contained slightly more carboxyls than corresponding humic fractions extracted by 0.1 mol L-1 NaOH, and the HA extracted by 0.1 mol L-1 NaOH after treatment with HF/HCI contained the least aromatics and carboxyls. The distribution of nitrogen functional groups of soil humic fractions studied was quite similar to each other and also quite similar to that of humic fraction from native soils. More than 75% of total N in each fraction was in amide form, with 9%-13% present as aromatic and/or aliphatic amines and the remainder as hoterocyclic N.
