Dear Editor,Influenza A viruses cause pandemics at an interval of approximately 10-40 years,and pigs are regarded as a＂mixing vessel＂because they are easily infected with avian and human influenza viruses（Ito et al...Dear Editor,Influenza A viruses cause pandemics at an interval of approximately 10-40 years,and pigs are regarded as a＂mixing vessel＂because they are easily infected with avian and human influenza viruses（Ito et al.,1998）.According to previous studies,H3N2,H1N2,and H1N1 subtypes o（swine influenza viruses have been detected in Korean pigs （Pascua et al., 2013; Kim et al., 2014; Song et al., 2007）. Moreover, a novel H3N2 influenza virus containing the matrix （34） gene from a 2009 pandemic influenza virus was detected in Korean pigs in 2013 （Pascua et al., 2013）, an H1N2 influenza virus con- taining the internal genes from a 2009 pandemic influ- enza virus was found in Korean pigs in 2014 （Kim et al., 2014）, and an H1N1 influenza virus containing all genes from the classical swine influenza viruses was isolated from Korean pigs in 2007 （Song et al., 2007）.展开更多
Objective: To investigate the prevalence and the risk factors of H9 N2 avian influenza among backyard birds in Iran between October and November 2015. Methods: In this study, a total of 15 500 blood samples and 2 884 ...Objective: To investigate the prevalence and the risk factors of H9 N2 avian influenza among backyard birds in Iran between October and November 2015. Methods: In this study, a total of 15 500 blood samples and 2 884 cloacal swab samples of backyard birds were collected in villages of Iran between October and November 2015. Then, serum samples were examined with the hemagluttination inhibition test and cloacal swab samples were pooled together and examined by RT-PCR. The samples that had serological titer ≥ 4(log2) and villages that had at least one seropositive sample were considered positive.Results: Out of 559 villages, 526(94.10%) were seropositive for the infection. Among 15 500 serum samples, 7 468(48.18%) samples were seropositive for the infection. The seroprevalence according to species was 54.02% among chickens, 17.59 % among ducks, 18.73% among turkeys, 84.21% among pigeons and 12.15% among ostriches, partridges and pheasants. Based on molecular test, 3.04% villages were positive. The seroprevalence in hot and humid area was less than that in cold and humid area(P<0.05).Conclusions: H9 N2 avian influenza has high seroprevalence among backyard birds of Iran. Therefore, preventive measures such as biosecurity Practices and monitoring should be applied to reduce the prevalence.展开更多
Due to isothermal heat treatment or welding,duplex stainless undergoes a variety of decomposition processes at a temperature ranging from 300-1100℃,which could form inter-metallic phases likeσ-phase and xphase,as we...Due to isothermal heat treatment or welding,duplex stainless undergoes a variety of decomposition processes at a temperature ranging from 300-1100℃,which could form inter-metallic phases likeσ-phase and xphase,as well as Cr_2N and M_(23)C_6,etc.These processes are not only influenced by temperature and cooling speed,but also are affected by alloy element content.The purpose of the research was to work out the qualitative and quantitative analysis of precipitate phases in 2205 duplex stainless steel with different isothermal heat treatment situation.After choosing the electrolyte and electrolytic systems,we use electrochemical method to extract precipitate phases from the matrix.Scanning electron microscope(SEM)and X-ray diffraction(XRD)were used to examine their modality and structure.Particle size distribution was performed by dynamic laser scattering sizer(DLS-Sizer).Furthermore,oxygen-nitrogen analyzer and carbon-sulfur analyzer were applied to measure the content of nitrogen and carbon in precipitate phases.Separation and quantitative analysis of precipitate phases were carried out by wet chemical analysis and inductively coupled plasma spectrometry(ICP).In a word,combining chemical and physical methods,our research reveals the transformation of precipitate phases'species,content,elemental composition and particle size distribution during different isothermal heat treatment situation.展开更多
为确定H9N2猪流感病毒(H9N2-SIV)通过瞬时受体电位通道M2(TRPM2)介导肺微血管上皮细胞(PMVEC)铁死亡的分子机制,使用H9N2-SIV接种PMVEC,构建TRPM2-siRNA质粒并转染细胞。用透射电镜观察细胞超微结构,用荧光探针法检测活性氧(ROS)、Ca^(...为确定H9N2猪流感病毒(H9N2-SIV)通过瞬时受体电位通道M2(TRPM2)介导肺微血管上皮细胞(PMVEC)铁死亡的分子机制,使用H9N2-SIV接种PMVEC,构建TRPM2-siRNA质粒并转染细胞。用透射电镜观察细胞超微结构,用荧光探针法检测活性氧(ROS)、Ca^(2+)和Fe^(2+);用生化试剂盒检测丙二醛(MDA)和谷胱甘肽(GSH)含量,并通过荧光定量PCR和Western-blot检测葡萄糖调节蛋白78(GRP78)、TRPM2、蛋白激酶R样内质网激酶(PERK)、活化转录因子4(ATF4)、阳离子转运调控样蛋白1(CHAC1)、谷胱甘肽过氧化物酶4(GPX4)的m RNA和蛋白表达水平。结果显示,H9N2-SIV感染可诱导细胞铁死亡,敲低TRPM2可以减少细胞内ROS水平,降低Ca^(2+)、Fe^(2+)及MDA含量,GSH水平明显增加;此外,GRP78、PERK、ATF4、CHAC1 m RNA和蛋白表达水平下调,GPX4的m RNA和蛋白表达水平上调。结果表明,H9N2-SIV感染可诱导细胞铁死亡,其可通过激活TRPM2使Ca^(2+)内流增多,进而激活PERK/ATF4/CHAC1信号通路,加速GSH耗竭,抑制GPX4的活性,促进细胞铁死亡。展开更多
基金in part funded by a 2015 research fund from Chungnam National University
文摘Dear Editor,Influenza A viruses cause pandemics at an interval of approximately 10-40 years,and pigs are regarded as a＂mixing vessel＂because they are easily infected with avian and human influenza viruses（Ito et al.,1998）.According to previous studies,H3N2,H1N2,and H1N1 subtypes o（swine influenza viruses have been detected in Korean pigs （Pascua et al., 2013; Kim et al., 2014; Song et al., 2007）. Moreover, a novel H3N2 influenza virus containing the matrix （34） gene from a 2009 pandemic influenza virus was detected in Korean pigs in 2013 （Pascua et al., 2013）, an H1N2 influenza virus con- taining the internal genes from a 2009 pandemic influ- enza virus was found in Korean pigs in 2014 （Kim et al., 2014）, and an H1N1 influenza virus containing all genes from the classical swine influenza viruses was isolated from Korean pigs in 2007 （Song et al., 2007）.
基金the Directorate of Health and Management of Poultry Disease of the Iranian Veterinary Organization for their support
文摘Objective: To investigate the prevalence and the risk factors of H9 N2 avian influenza among backyard birds in Iran between October and November 2015. Methods: In this study, a total of 15 500 blood samples and 2 884 cloacal swab samples of backyard birds were collected in villages of Iran between October and November 2015. Then, serum samples were examined with the hemagluttination inhibition test and cloacal swab samples were pooled together and examined by RT-PCR. The samples that had serological titer ≥ 4(log2) and villages that had at least one seropositive sample were considered positive.Results: Out of 559 villages, 526(94.10%) were seropositive for the infection. Among 15 500 serum samples, 7 468(48.18%) samples were seropositive for the infection. The seroprevalence according to species was 54.02% among chickens, 17.59 % among ducks, 18.73% among turkeys, 84.21% among pigeons and 12.15% among ostriches, partridges and pheasants. Based on molecular test, 3.04% villages were positive. The seroprevalence in hot and humid area was less than that in cold and humid area(P<0.05).Conclusions: H9 N2 avian influenza has high seroprevalence among backyard birds of Iran. Therefore, preventive measures such as biosecurity Practices and monitoring should be applied to reduce the prevalence.
文摘Due to isothermal heat treatment or welding,duplex stainless undergoes a variety of decomposition processes at a temperature ranging from 300-1100℃,which could form inter-metallic phases likeσ-phase and xphase,as well as Cr_2N and M_(23)C_6,etc.These processes are not only influenced by temperature and cooling speed,but also are affected by alloy element content.The purpose of the research was to work out the qualitative and quantitative analysis of precipitate phases in 2205 duplex stainless steel with different isothermal heat treatment situation.After choosing the electrolyte and electrolytic systems,we use electrochemical method to extract precipitate phases from the matrix.Scanning electron microscope(SEM)and X-ray diffraction(XRD)were used to examine their modality and structure.Particle size distribution was performed by dynamic laser scattering sizer(DLS-Sizer).Furthermore,oxygen-nitrogen analyzer and carbon-sulfur analyzer were applied to measure the content of nitrogen and carbon in precipitate phases.Separation and quantitative analysis of precipitate phases were carried out by wet chemical analysis and inductively coupled plasma spectrometry(ICP).In a word,combining chemical and physical methods,our research reveals the transformation of precipitate phases'species,content,elemental composition and particle size distribution during different isothermal heat treatment situation.
文摘为确定H9N2猪流感病毒(H9N2-SIV)通过瞬时受体电位通道M2(TRPM2)介导肺微血管上皮细胞(PMVEC)铁死亡的分子机制,使用H9N2-SIV接种PMVEC,构建TRPM2-siRNA质粒并转染细胞。用透射电镜观察细胞超微结构,用荧光探针法检测活性氧(ROS)、Ca^(2+)和Fe^(2+);用生化试剂盒检测丙二醛(MDA)和谷胱甘肽(GSH)含量,并通过荧光定量PCR和Western-blot检测葡萄糖调节蛋白78(GRP78)、TRPM2、蛋白激酶R样内质网激酶(PERK)、活化转录因子4(ATF4)、阳离子转运调控样蛋白1(CHAC1)、谷胱甘肽过氧化物酶4(GPX4)的m RNA和蛋白表达水平。结果显示,H9N2-SIV感染可诱导细胞铁死亡,敲低TRPM2可以减少细胞内ROS水平,降低Ca^(2+)、Fe^(2+)及MDA含量,GSH水平明显增加;此外,GRP78、PERK、ATF4、CHAC1 m RNA和蛋白表达水平下调,GPX4的m RNA和蛋白表达水平上调。结果表明,H9N2-SIV感染可诱导细胞铁死亡,其可通过激活TRPM2使Ca^(2+)内流增多,进而激活PERK/ATF4/CHAC1信号通路,加速GSH耗竭,抑制GPX4的活性,促进细胞铁死亡。
