Seven undescribed compounds,including three flavones(1-3),one phenylpropanoid(19),three monoaromatic hydrocarbons(27-29),were isolated from the twigs of Mosla chinensis Maxim together with twenty-eight known compounds...Seven undescribed compounds,including three flavones(1-3),one phenylpropanoid(19),three monoaromatic hydrocarbons(27-29),were isolated from the twigs of Mosla chinensis Maxim together with twenty-eight known compounds.The structures were characterized by HRESIMS,1D and 2D NMR,and ECD spectroscopic techniques.Compound 20 displayed the most significant activity against A/WSN/33/2009(H1N1)virus(IC_(50)=20.47μM)compared to the positive control oseltamivir(IC_(50)=6.85μM).Further research on the anti-influenza mechanism showed that compound 20 could bind to H1N1 virus surface antigen HA1 and inhibit the early attachment stage of the virus.Furthermore,compounds 9,22,23,and 25 displayed moderate inhibitory effects on the NO expression in LPS inducing Raw 264.7 cells with IC50 values of 22.78,20.47,27.66,and 30.14μM,respectively.展开更多
The increasingly serious problem of acid rain is leading to increased potassium （K） loss from soils, and in our field investigation, we found that even congenerically relative Mosla species show different tolerance ...The increasingly serious problem of acid rain is leading to increased potassium （K） loss from soils, and in our field investigation, we found that even congenerically relative Mosla species show different tolerance to K-deficiency. A hydroponic study was conducted on the growth of two Mosla species and their morphological, physiological and stoichiometric traits in response to limited （0.35 mmol K/L）, normal （3.25 mmol K/L） and excessive （6.50 mmol K/L） K concentrations. Mosla hangchowensis is an endangered plant, whereas Mosla dianthera a widespread weed. In the case of M. hangchowensis, in comparison with normal K concentration, K-limitation induced a significant reduction in net photosynthetic rate （Pn）, soluble protein content, and superoxide dismutase （SOD） actix, ity, but an increase in malondialdehyde （MDA） concentration. However, leaf mass ratio （LMR） and root mass ratio （RMR） were changed little by K-limitation. In contrast, for M. dianthera, K-limitation had little effect on Pn, soluble protein content, SOD activity, and MDA concentration, but increased LMR and RMR. Critical values of N （nitrogen）：K and K：P （phosphorus） ratios in the shoots indicated that limitation in acquiring K occurred under K-limited conditions for M. hangchowensis but not for M. dianthera. We found that low K content in natural habitats was a restrictive factor in the growth and distribution of M.. hangchowensis, and soil K-deficiency caused by acid rain worsened the situation of M. hangchowensis, while M. dianthera could well acclimate to the increasing K-deficiency. We suggest that controlling the acid rain and applying K fertilizers may be an effective way to rescue the endangered M. hangchowensis.展开更多
The volatile compounds emitted from Mosla chinensis Maxim were analyzed by headspace solid-phase micro- extraction （HS-SPME） and headspace liquid-phase microextraction （HS-LPME） combined with gas chromatography-ma...The volatile compounds emitted from Mosla chinensis Maxim were analyzed by headspace solid-phase micro- extraction （HS-SPME） and headspace liquid-phase microextraction （HS-LPME） combined with gas chromatography-mass spectrometry （GC-MS）. The main volatiles from Mosla chinensis Maxim were studied in this paper. It can be seen that 61 compounds were separated and identified. Forty-nine volatile compounds were identified by SPME method, mainly including myrcene, a-terpinene, p-cymene, （E）-ocimene, thymol, thymol acetate and （E）-fl-farnesene. Forty-five major volatile compounds were identified by LPME method, including a-thujene, a-pinene, camphene, butanoic acid, 2-methylpropyl ester, myrcene, butanoic acid, butyl ester, a-terpinene, p-cymene, （E）-ocimene, butane, 1,1-dibutoxy-, thymol, thymol acetate and （E）-fl-farnesene. After analyzing the volatile compounds, multiple linear regression （MLR） method was used for building the regression model. Then the quantitative structure-retention relationship （QSRR） model was validated by predictive-ability test. The prediction results were in good agreement with the experimental values. The results demonstrated that headspace SPME-GC-MS and LPME-GC-MS are the simple, rapid and easy sample enrichment technique suitable for analysis of volatile compounds. This investigation provided an effective method for predicting the retention indices of new compounds even in the absence of the standard candidates.展开更多
The aerial parts of Mosla chinensis Maxim.and Mosla chinensis cv.'Jiangxiangru'(MCJ)are widely utilized in traditional Chinese medicine(TCM),known collectively as Xiang-ru.However,due to clinical effectiveness...The aerial parts of Mosla chinensis Maxim.and Mosla chinensis cv.'Jiangxiangru'(MCJ)are widely utilized in traditional Chinese medicine(TCM),known collectively as Xiang-ru.However,due to clinical effectiveness concerns and frequent misidentification,the original plants have increasingly been substituted by various species within the genera Elsholtzia and Mosla.The challenge in distinguishing between these genera arises from their similar morphological and metabolic profiles.To address this issue,our study introduced a rapid method for metabolic characterization,employing high-resolution mass spectrometry-based metabolomics.Through detailed biosynthetic and chemometric analyses,we pinpointed five phenolic compounds—salviaflaside,cynaroside,scutellarein-7-O-D-glucoside,rutin,and vicenin-2—among 203 identified compounds,as reliable chemical markers for distinguishing Xiang-ru from closely related Elsholtzia species.This methodology holds promise for broad application in the analysis of plant aerial parts,especially in verifying the authenticity of aromatic traditional medicinal plants.Our findings underscore the importance of non-volatile compounds as dependable chemical markers in the authentication process of aromatic traditional medicinal plants.展开更多
基金financed by National Natural Science Foundation of China(No.31660100)Innovative Team of Yunnan Province(No.2019HC018)the key Project of Yunnan Province(No.202103AC10005,No.202302AG050004).
文摘Seven undescribed compounds,including three flavones(1-3),one phenylpropanoid(19),three monoaromatic hydrocarbons(27-29),were isolated from the twigs of Mosla chinensis Maxim together with twenty-eight known compounds.The structures were characterized by HRESIMS,1D and 2D NMR,and ECD spectroscopic techniques.Compound 20 displayed the most significant activity against A/WSN/33/2009(H1N1)virus(IC_(50)=20.47μM)compared to the positive control oseltamivir(IC_(50)=6.85μM).Further research on the anti-influenza mechanism showed that compound 20 could bind to H1N1 virus surface antigen HA1 and inhibit the early attachment stage of the virus.Furthermore,compounds 9,22,23,and 25 displayed moderate inhibitory effects on the NO expression in LPS inducing Raw 264.7 cells with IC50 values of 22.78,20.47,27.66,and 30.14μM,respectively.
基金supported by the National Natural Science Foundation of China (No.30570113)the Research Fund for the Doctoral Program of Higher Education (No.20060335008),China
文摘The increasingly serious problem of acid rain is leading to increased potassium （K） loss from soils, and in our field investigation, we found that even congenerically relative Mosla species show different tolerance to K-deficiency. A hydroponic study was conducted on the growth of two Mosla species and their morphological, physiological and stoichiometric traits in response to limited （0.35 mmol K/L）, normal （3.25 mmol K/L） and excessive （6.50 mmol K/L） K concentrations. Mosla hangchowensis is an endangered plant, whereas Mosla dianthera a widespread weed. In the case of M. hangchowensis, in comparison with normal K concentration, K-limitation induced a significant reduction in net photosynthetic rate （Pn）, soluble protein content, and superoxide dismutase （SOD） actix, ity, but an increase in malondialdehyde （MDA） concentration. However, leaf mass ratio （LMR） and root mass ratio （RMR） were changed little by K-limitation. In contrast, for M. dianthera, K-limitation had little effect on Pn, soluble protein content, SOD activity, and MDA concentration, but increased LMR and RMR. Critical values of N （nitrogen）：K and K：P （phosphorus） ratios in the shoots indicated that limitation in acquiring K occurred under K-limited conditions for M. hangchowensis but not for M. dianthera. We found that low K content in natural habitats was a restrictive factor in the growth and distribution of M.. hangchowensis, and soil K-deficiency caused by acid rain worsened the situation of M. hangchowensis, while M. dianthera could well acclimate to the increasing K-deficiency. We suggest that controlling the acid rain and applying K fertilizers may be an effective way to rescue the endangered M. hangchowensis.
基金Project supported by the Natural Science Foundation Programof Zhejiang Province （No. Y407308）, the Ministry of Science and Technology of Zhejiang Province （No. 201 OR 10044） and the Sprout Talented Project Program of Zhejiang Province （No. 2008R40G2020019）.
文摘The volatile compounds emitted from Mosla chinensis Maxim were analyzed by headspace solid-phase micro- extraction （HS-SPME） and headspace liquid-phase microextraction （HS-LPME） combined with gas chromatography-mass spectrometry （GC-MS）. The main volatiles from Mosla chinensis Maxim were studied in this paper. It can be seen that 61 compounds were separated and identified. Forty-nine volatile compounds were identified by SPME method, mainly including myrcene, a-terpinene, p-cymene, （E）-ocimene, thymol, thymol acetate and （E）-fl-farnesene. Forty-five major volatile compounds were identified by LPME method, including a-thujene, a-pinene, camphene, butanoic acid, 2-methylpropyl ester, myrcene, butanoic acid, butyl ester, a-terpinene, p-cymene, （E）-ocimene, butane, 1,1-dibutoxy-, thymol, thymol acetate and （E）-fl-farnesene. After analyzing the volatile compounds, multiple linear regression （MLR） method was used for building the regression model. Then the quantitative structure-retention relationship （QSRR） model was validated by predictive-ability test. The prediction results were in good agreement with the experimental values. The results demonstrated that headspace SPME-GC-MS and LPME-GC-MS are the simple, rapid and easy sample enrichment technique suitable for analysis of volatile compounds. This investigation provided an effective method for predicting the retention indices of new compounds even in the absence of the standard candidates.
基金supported by the National Key R&D Program of China(No.2022YFC3501700)the Young Elite Scientists Sponsorship Program by Cast(No.2021-QNRC1-02)+1 种基金the Key Project at Central Government Level:The Ability Establishment of Sustainable Use for Valuable Chinese Medicine Resources(No.2060302)the Research Project of Science and Technology Commission of Shanghai Municipalit(No.21DZ2202300)。
文摘The aerial parts of Mosla chinensis Maxim.and Mosla chinensis cv.'Jiangxiangru'(MCJ)are widely utilized in traditional Chinese medicine(TCM),known collectively as Xiang-ru.However,due to clinical effectiveness concerns and frequent misidentification,the original plants have increasingly been substituted by various species within the genera Elsholtzia and Mosla.The challenge in distinguishing between these genera arises from their similar morphological and metabolic profiles.To address this issue,our study introduced a rapid method for metabolic characterization,employing high-resolution mass spectrometry-based metabolomics.Through detailed biosynthetic and chemometric analyses,we pinpointed five phenolic compounds—salviaflaside,cynaroside,scutellarein-7-O-D-glucoside,rutin,and vicenin-2—among 203 identified compounds,as reliable chemical markers for distinguishing Xiang-ru from closely related Elsholtzia species.This methodology holds promise for broad application in the analysis of plant aerial parts,especially in verifying the authenticity of aromatic traditional medicinal plants.Our findings underscore the importance of non-volatile compounds as dependable chemical markers in the authentication process of aromatic traditional medicinal plants.
