The increasingly serious problem of acid rain is leading to increased potassium （K） loss from soils, and in our field investigation, we found that even congenerically relative Mosla species show different tolerance ...The increasingly serious problem of acid rain is leading to increased potassium （K） loss from soils, and in our field investigation, we found that even congenerically relative Mosla species show different tolerance to K-deficiency. A hydroponic study was conducted on the growth of two Mosla species and their morphological, physiological and stoichiometric traits in response to limited （0.35 mmol K/L）, normal （3.25 mmol K/L） and excessive （6.50 mmol K/L） K concentrations. Mosla hangchowensis is an endangered plant, whereas Mosla dianthera a widespread weed. In the case of M. hangchowensis, in comparison with normal K concentration, K-limitation induced a significant reduction in net photosynthetic rate （Pn）, soluble protein content, and superoxide dismutase （SOD） actix, ity, but an increase in malondialdehyde （MDA） concentration. However, leaf mass ratio （LMR） and root mass ratio （RMR） were changed little by K-limitation. In contrast, for M. dianthera, K-limitation had little effect on Pn, soluble protein content, SOD activity, and MDA concentration, but increased LMR and RMR. Critical values of N （nitrogen）：K and K：P （phosphorus） ratios in the shoots indicated that limitation in acquiring K occurred under K-limited conditions for M. hangchowensis but not for M. dianthera. We found that low K content in natural habitats was a restrictive factor in the growth and distribution of M.. hangchowensis, and soil K-deficiency caused by acid rain worsened the situation of M. hangchowensis, while M. dianthera could well acclimate to the increasing K-deficiency. We suggest that controlling the acid rain and applying K fertilizers may be an effective way to rescue the endangered M. hangchowensis.展开更多
Seven undescribed compounds,including three flavones(1-3),one phenylpropanoid(19),three monoaromatic hydrocarbons(27-29),were isolated from the twigs of Mosla chinensis Maxim together with twenty-eight known compounds...Seven undescribed compounds,including three flavones(1-3),one phenylpropanoid(19),three monoaromatic hydrocarbons(27-29),were isolated from the twigs of Mosla chinensis Maxim together with twenty-eight known compounds.The structures were characterized by HRESIMS,1D and 2D NMR,and ECD spectroscopic techniques.Compound 20 displayed the most significant activity against A/WSN/33/2009(H1N1)virus(IC_(50)=20.47μM)compared to the positive control oseltamivir(IC_(50)=6.85μM).Further research on the anti-influenza mechanism showed that compound 20 could bind to H1N1 virus surface antigen HA1 and inhibit the early attachment stage of the virus.Furthermore,compounds 9,22,23,and 25 displayed moderate inhibitory effects on the NO expression in LPS inducing Raw 264.7 cells with IC50 values of 22.78,20.47,27.66,and 30.14μM,respectively.展开更多
The volatile compounds emitted from Mosla chinensis Maxim were analyzed by headspace solid-phase micro- extraction （HS-SPME） and headspace liquid-phase microextraction （HS-LPME） combined with gas chromatography-ma...The volatile compounds emitted from Mosla chinensis Maxim were analyzed by headspace solid-phase micro- extraction （HS-SPME） and headspace liquid-phase microextraction （HS-LPME） combined with gas chromatography-mass spectrometry （GC-MS）. The main volatiles from Mosla chinensis Maxim were studied in this paper. It can be seen that 61 compounds were separated and identified. Forty-nine volatile compounds were identified by SPME method, mainly including myrcene, a-terpinene, p-cymene, （E）-ocimene, thymol, thymol acetate and （E）-fl-farnesene. Forty-five major volatile compounds were identified by LPME method, including a-thujene, a-pinene, camphene, butanoic acid, 2-methylpropyl ester, myrcene, butanoic acid, butyl ester, a-terpinene, p-cymene, （E）-ocimene, butane, 1,1-dibutoxy-, thymol, thymol acetate and （E）-fl-farnesene. After analyzing the volatile compounds, multiple linear regression （MLR） method was used for building the regression model. Then the quantitative structure-retention relationship （QSRR） model was validated by predictive-ability test. The prediction results were in good agreement with the experimental values. The results demonstrated that headspace SPME-GC-MS and LPME-GC-MS are the simple, rapid and easy sample enrichment technique suitable for analysis of volatile compounds. This investigation provided an effective method for predicting the retention indices of new compounds even in the absence of the standard candidates.展开更多
目的探讨轻悦饮料对小鼠的抗抑郁作用,并基于网络药理学和分子对接技术初步揭示其改善肥胖相关抑郁症的分子机制。方法通过悬尾实验、旷场实验和糖水偏好实验评价轻悦饮料的抗抑郁活性;利用网络药理学筛选复方活性成分及靶点,构建“成分...目的探讨轻悦饮料对小鼠的抗抑郁作用,并基于网络药理学和分子对接技术初步揭示其改善肥胖相关抑郁症的分子机制。方法通过悬尾实验、旷场实验和糖水偏好实验评价轻悦饮料的抗抑郁活性;利用网络药理学筛选复方活性成分及靶点,构建“成分-靶点-通路”网络,结合STRING数据库构建蛋白互作(protein-protein interaction,PPI)网络,通过Cytoscape 3.9.1软件筛选核心靶点;采用Metascape平台进行京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)通路富集分析,并用AutoDock和PyMOL验证核心成分与关键靶点的分子对接活性。结果行为学实验显示,轻悦饮料可显著缩短悬尾实验中小鼠的不动时间(P<0.05),并增加旷场实验中中心格运动时间、减少中心格停留时间(P<0.01)。网络药理学筛选得到55种活性成分(如槲皮素、金合欢素、木犀草素)及316个潜在靶点,核心靶点包括AKT1、PPARG、过氧化氢酶(catalase,CAT)、白细胞介素(interleukin,IL)-1β、FOS,主要富集于癌症通路、PI3K-Akt信号通路、HIF-1信号通路等。分子对接验证显示,核心成分与关键靶点具有良好的结合活性(结合能≤-5.0 kcal/mol)。结论轻悦饮料可能通过多成分(槲皮素、木犀草素等)、多靶点(AKT1、PPARG等)协同作用于PI3K-Akt等相关信号通路,发挥抗抑郁及改善肥胖相关抑郁症的作用,体现了中药复方“整体调节”的特点。展开更多
基金supported by the National Natural Science Foundation of China (No.30570113)the Research Fund for the Doctoral Program of Higher Education (No.20060335008),China
文摘The increasingly serious problem of acid rain is leading to increased potassium （K） loss from soils, and in our field investigation, we found that even congenerically relative Mosla species show different tolerance to K-deficiency. A hydroponic study was conducted on the growth of two Mosla species and their morphological, physiological and stoichiometric traits in response to limited （0.35 mmol K/L）, normal （3.25 mmol K/L） and excessive （6.50 mmol K/L） K concentrations. Mosla hangchowensis is an endangered plant, whereas Mosla dianthera a widespread weed. In the case of M. hangchowensis, in comparison with normal K concentration, K-limitation induced a significant reduction in net photosynthetic rate （Pn）, soluble protein content, and superoxide dismutase （SOD） actix, ity, but an increase in malondialdehyde （MDA） concentration. However, leaf mass ratio （LMR） and root mass ratio （RMR） were changed little by K-limitation. In contrast, for M. dianthera, K-limitation had little effect on Pn, soluble protein content, SOD activity, and MDA concentration, but increased LMR and RMR. Critical values of N （nitrogen）：K and K：P （phosphorus） ratios in the shoots indicated that limitation in acquiring K occurred under K-limited conditions for M. hangchowensis but not for M. dianthera. We found that low K content in natural habitats was a restrictive factor in the growth and distribution of M.. hangchowensis, and soil K-deficiency caused by acid rain worsened the situation of M. hangchowensis, while M. dianthera could well acclimate to the increasing K-deficiency. We suggest that controlling the acid rain and applying K fertilizers may be an effective way to rescue the endangered M. hangchowensis.
基金financed by National Natural Science Foundation of China(No.31660100)Innovative Team of Yunnan Province(No.2019HC018)the key Project of Yunnan Province(No.202103AC10005,No.202302AG050004).
文摘Seven undescribed compounds,including three flavones(1-3),one phenylpropanoid(19),three monoaromatic hydrocarbons(27-29),were isolated from the twigs of Mosla chinensis Maxim together with twenty-eight known compounds.The structures were characterized by HRESIMS,1D and 2D NMR,and ECD spectroscopic techniques.Compound 20 displayed the most significant activity against A/WSN/33/2009(H1N1)virus(IC_(50)=20.47μM)compared to the positive control oseltamivir(IC_(50)=6.85μM).Further research on the anti-influenza mechanism showed that compound 20 could bind to H1N1 virus surface antigen HA1 and inhibit the early attachment stage of the virus.Furthermore,compounds 9,22,23,and 25 displayed moderate inhibitory effects on the NO expression in LPS inducing Raw 264.7 cells with IC50 values of 22.78,20.47,27.66,and 30.14μM,respectively.
基金Project supported by the Natural Science Foundation Programof Zhejiang Province （No. Y407308）, the Ministry of Science and Technology of Zhejiang Province （No. 201 OR 10044） and the Sprout Talented Project Program of Zhejiang Province （No. 2008R40G2020019）.
文摘The volatile compounds emitted from Mosla chinensis Maxim were analyzed by headspace solid-phase micro- extraction （HS-SPME） and headspace liquid-phase microextraction （HS-LPME） combined with gas chromatography-mass spectrometry （GC-MS）. The main volatiles from Mosla chinensis Maxim were studied in this paper. It can be seen that 61 compounds were separated and identified. Forty-nine volatile compounds were identified by SPME method, mainly including myrcene, a-terpinene, p-cymene, （E）-ocimene, thymol, thymol acetate and （E）-fl-farnesene. Forty-five major volatile compounds were identified by LPME method, including a-thujene, a-pinene, camphene, butanoic acid, 2-methylpropyl ester, myrcene, butanoic acid, butyl ester, a-terpinene, p-cymene, （E）-ocimene, butane, 1,1-dibutoxy-, thymol, thymol acetate and （E）-fl-farnesene. After analyzing the volatile compounds, multiple linear regression （MLR） method was used for building the regression model. Then the quantitative structure-retention relationship （QSRR） model was validated by predictive-ability test. The prediction results were in good agreement with the experimental values. The results demonstrated that headspace SPME-GC-MS and LPME-GC-MS are the simple, rapid and easy sample enrichment technique suitable for analysis of volatile compounds. This investigation provided an effective method for predicting the retention indices of new compounds even in the absence of the standard candidates.
文摘目的探讨轻悦饮料对小鼠的抗抑郁作用,并基于网络药理学和分子对接技术初步揭示其改善肥胖相关抑郁症的分子机制。方法通过悬尾实验、旷场实验和糖水偏好实验评价轻悦饮料的抗抑郁活性;利用网络药理学筛选复方活性成分及靶点,构建“成分-靶点-通路”网络,结合STRING数据库构建蛋白互作(protein-protein interaction,PPI)网络,通过Cytoscape 3.9.1软件筛选核心靶点;采用Metascape平台进行京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)通路富集分析,并用AutoDock和PyMOL验证核心成分与关键靶点的分子对接活性。结果行为学实验显示,轻悦饮料可显著缩短悬尾实验中小鼠的不动时间(P<0.05),并增加旷场实验中中心格运动时间、减少中心格停留时间(P<0.01)。网络药理学筛选得到55种活性成分(如槲皮素、金合欢素、木犀草素)及316个潜在靶点,核心靶点包括AKT1、PPARG、过氧化氢酶(catalase,CAT)、白细胞介素(interleukin,IL)-1β、FOS,主要富集于癌症通路、PI3K-Akt信号通路、HIF-1信号通路等。分子对接验证显示,核心成分与关键靶点具有良好的结合活性(结合能≤-5.0 kcal/mol)。结论轻悦饮料可能通过多成分(槲皮素、木犀草素等)、多靶点(AKT1、PPARG等)协同作用于PI3K-Akt等相关信号通路,发挥抗抑郁及改善肥胖相关抑郁症的作用,体现了中药复方“整体调节”的特点。
