Morphological and molecular data confirm a new species in genus Malcus from Xizang,China.Malcus zhengi sp.nov.is described and illustrated.Photos and illustrations of the adult,critical morphological characteristics a...Morphological and molecular data confirm a new species in genus Malcus from Xizang,China.Malcus zhengi sp.nov.is described and illustrated.Photos and illustrations of the adult,critical morphological characteristics and pygophore are provided.展开更多
The Hippoboscidae(Diptera:Calyptratae:Hippoboscoidea),or louse flies,are ectoparasites that suck blood of their avian or mammalian hosts with strong host specificity.Therefore,they are of great interest in evolutionar...The Hippoboscidae(Diptera:Calyptratae:Hippoboscoidea),or louse flies,are ectoparasites that suck blood of their avian or mammalian hosts with strong host specificity.Therefore,they are of great interest in evolutionary research.Here,we sequenced the mitochondrial genome of Ornithophila metallica(Schiner,1864)for the first time.It is 15,739 bp in length and possesses 37 mitochondrial genes,comprising 13 protein-coding genes(PCGs),22 tRNA and two rRNA genes,and a non-coding control region.The gene arrangement is consistent with that of most Dipteran mitochondrial genomes.The mitogenome is subsequently used for phylogeny reconstruction using all available mitogenomes of Hippoboscoidea.The Hippoboscidae is recovered as a monophyletic group,sister to the clade of(streblid+Nycteribiidae),which is the sister group to the monophyletic Glossinidae.Within the Hippoboscidae,the Ornithophila clusters together with Ornithomya,which is a sister group to(Melophagus+Lipoptena).This study broadens our knowledge of the Hippoboscidae and contributes to research in entomology,veterinary science,and ecology.展开更多
Aleuropteryx sinica Liu&Yang,2003 is the only species of Aleuropteryx in the family Coniopterygidae found in China.In this study,we used high-throughput sequencing methods to assemble the complete mitochondrial ge...Aleuropteryx sinica Liu&Yang,2003 is the only species of Aleuropteryx in the family Coniopterygidae found in China.In this study,we used high-throughput sequencing methods to assemble the complete mitochondrial genome of A.sinica.The results showed that the complete mitogenome is 15,600 bp in length and had a high AT content(76.30%),with a typical set of 37 genes,including 13 protein-coding genes(PCGs),22 transfer RNA genes(tRNAs),2 ribosomal RNA genes(rRNAs),and 1 control region(CR)Based on the sequences of 13PCGs+2rRNAs,the phylogenetic analysis revealed that A.sinica,Coniopteryx sp.,Conwentzia sinica Yang,1974,Semidalis macleodi Meinander,1972 and Semidalis aleyrodiformis Stephens,1836 form a clade,which is a sister group to other families of Neuroptera.展开更多
The detection and parameterization of molecular clumps are the first step in studying them.We propose a method based on the Local Density Clustering algorithm while physical parameters of those clumps are measured usi...The detection and parameterization of molecular clumps are the first step in studying them.We propose a method based on the Local Density Clustering algorithm while physical parameters of those clumps are measured using the Multiple Gaussian Model algorithm.One advantage of applying the Local Density Clustering to the clump detection and segmentation,is the high accuracy under different signal-to-noise levels.The Multiple Gaussian Model is able to deal with overlapping clumps whose parameters can reliably be derived.Using simulation and synthetic data,we have verified that the proposed algorithm could accurately characterize the morphology and flux of molecular clumps.The total flux recovery rate in 13CO(J=1-0)line of M16 is measured as 90.2%.The detection rate and the completeness limit are 81.7%and 20 K km s-1 in 13CO(J=1-0)line of M16,respectively.展开更多
本研究旨在通过Meta分析探究饲粮添加白藜芦醇对猪肉品质的影响,并整合转录组数据挖掘与计算生物学方法揭示其潜在调控机制。系统检索2000年1月至2025年1月期间在中国知网、万方、Web of Science和Science Direct数据库中发表的白藜芦...本研究旨在通过Meta分析探究饲粮添加白藜芦醇对猪肉品质的影响,并整合转录组数据挖掘与计算生物学方法揭示其潜在调控机制。系统检索2000年1月至2025年1月期间在中国知网、万方、Web of Science和Science Direct数据库中发表的白藜芦醇对猪肉品质影响的相关文献,依据纳入与排除标准进行筛选后,最终纳入5项研究用于Meta分析。Meta分析结果表明,与对照组相比,饲粮添加白藜芦醇可显著提高屠宰后45 min猪肉红度值(P<0.05),显著降低屠宰后24 h猪肉亮度值(P<0.05),并显著降低猪肉滴水损失(P<0.05)。进一步通过转录组数据挖掘,筛选出与猪肉红度值和滴水损失相关的潜在基因,并与白藜芦醇的预测靶点进行交集分析,结果表明,白藜芦醇可能通过调控碳酸酐酶2(CA2)、膜联蛋白A5(ANXA5)、半胱氨酸-天冬氨酸蛋白酶7(CASP7)、11β-羟类固醇脱氢酶1(HSD11B1)和热休克蛋白90α家族A类成员1(HSP90AA1)的表达影响猪肉滴水损失;同时,可能通过调控雌激素受体1(ESR1)和CASP7的表达影响猪肉红度值。分子对接与分子动力学模拟进一步证实,白藜芦醇与上述基因编码的蛋白存在潜在结合作用。综上所述,饲粮添加白藜芦醇对猪肉品质的改善作用主要为提高红度值、降低亮度值和滴水损失,其调控猪肉滴水损失的潜在靶点为CA2、ANXA5、CASP7、HSD11B1和HSP90AA1,调控猪肉红度值的潜在靶点为ESR1和CASP7。这些发现可为后续基于上述潜在靶点解析白藜芦醇调控猪肉品质的机制研究提供依据。展开更多
The completion of genome sequences and subsequent high-throughput mapping of molecular networks have allowed us to study biology from the network perspective. Experimental, statistical and mathematical modeling approa...The completion of genome sequences and subsequent high-throughput mapping of molecular networks have allowed us to study biology from the network perspective. Experimental, statistical and mathematical modeling approaches have been employed to study the structure, function and dynamics of molecular networks, and begin to reveal important links of various network properties to the functions of the biological systems. In agreement with these functional links, evolutionary selection of a network is apparently based on the function, rather than directly on the structure of the network. Dynamic modularity is one of the prominent features of molecular networks. Taking advantage of such a feature may simplify network-based biological studies through construction of process-specific modular networks and provide functional and mechanistic insights linking genotypic variations to complex traits or diseases, which is likely to be a key approach in the next wave of understanding complex human diseases. With the development of ready-to-use network analysis and modeling tools the networks approaches will be infused into everyday biological research in the near future.展开更多
基金This study received financial support from the National Natural Science Foundation of China(31820103013,31430079)the Project of Ministry of Science and Technology of the People’s Republic of China(2015FY210300).
文摘Morphological and molecular data confirm a new species in genus Malcus from Xizang,China.Malcus zhengi sp.nov.is described and illustrated.Photos and illustrations of the adult,critical morphological characteristics and pygophore are provided.
基金funded by the National Natural Science Foundation of China(3220036532170450)the Fundamental Research Funds for the Central Universities(JCYJ202511).
文摘The Hippoboscidae(Diptera:Calyptratae:Hippoboscoidea),or louse flies,are ectoparasites that suck blood of their avian or mammalian hosts with strong host specificity.Therefore,they are of great interest in evolutionary research.Here,we sequenced the mitochondrial genome of Ornithophila metallica(Schiner,1864)for the first time.It is 15,739 bp in length and possesses 37 mitochondrial genes,comprising 13 protein-coding genes(PCGs),22 tRNA and two rRNA genes,and a non-coding control region.The gene arrangement is consistent with that of most Dipteran mitochondrial genomes.The mitogenome is subsequently used for phylogeny reconstruction using all available mitogenomes of Hippoboscoidea.The Hippoboscidae is recovered as a monophyletic group,sister to the clade of(streblid+Nycteribiidae),which is the sister group to the monophyletic Glossinidae.Within the Hippoboscidae,the Ornithophila clusters together with Ornithomya,which is a sister group to(Melophagus+Lipoptena).This study broadens our knowledge of the Hippoboscidae and contributes to research in entomology,veterinary science,and ecology.
基金supported by Beijing Natural Science Foundation(5224042).
文摘Aleuropteryx sinica Liu&Yang,2003 is the only species of Aleuropteryx in the family Coniopterygidae found in China.In this study,we used high-throughput sequencing methods to assemble the complete mitochondrial genome of A.sinica.The results showed that the complete mitogenome is 15,600 bp in length and had a high AT content(76.30%),with a typical set of 37 genes,including 13 protein-coding genes(PCGs),22 transfer RNA genes(tRNAs),2 ribosomal RNA genes(rRNAs),and 1 control region(CR)Based on the sequences of 13PCGs+2rRNAs,the phylogenetic analysis revealed that A.sinica,Coniopteryx sp.,Conwentzia sinica Yang,1974,Semidalis macleodi Meinander,1972 and Semidalis aleyrodiformis Stephens,1836 form a clade,which is a sister group to other families of Neuroptera.
基金Supported by the National Natural Science Foundation of China。
文摘The detection and parameterization of molecular clumps are the first step in studying them.We propose a method based on the Local Density Clustering algorithm while physical parameters of those clumps are measured using the Multiple Gaussian Model algorithm.One advantage of applying the Local Density Clustering to the clump detection and segmentation,is the high accuracy under different signal-to-noise levels.The Multiple Gaussian Model is able to deal with overlapping clumps whose parameters can reliably be derived.Using simulation and synthetic data,we have verified that the proposed algorithm could accurately characterize the morphology and flux of molecular clumps.The total flux recovery rate in 13CO(J=1-0)line of M16 is measured as 90.2%.The detection rate and the completeness limit are 81.7%and 20 K km s-1 in 13CO(J=1-0)line of M16,respectively.
文摘The completion of genome sequences and subsequent high-throughput mapping of molecular networks have allowed us to study biology from the network perspective. Experimental, statistical and mathematical modeling approaches have been employed to study the structure, function and dynamics of molecular networks, and begin to reveal important links of various network properties to the functions of the biological systems. In agreement with these functional links, evolutionary selection of a network is apparently based on the function, rather than directly on the structure of the network. Dynamic modularity is one of the prominent features of molecular networks. Taking advantage of such a feature may simplify network-based biological studies through construction of process-specific modular networks and provide functional and mechanistic insights linking genotypic variations to complex traits or diseases, which is likely to be a key approach in the next wave of understanding complex human diseases. With the development of ready-to-use network analysis and modeling tools the networks approaches will be infused into everyday biological research in the near future.
