目的探讨MN/CAⅨ在肾细胞癌组织和血液中的表达情况和诊断价值。方法采用RT-PCR技术检测62例肾细胞癌患者肿瘤组织和外周血液中MN/CA IX mRNA的表达,以32例正常肾组织和非肾癌患者外周血作为对照。采用免疫组化技术检测36例肾透明细胞癌...目的探讨MN/CAⅨ在肾细胞癌组织和血液中的表达情况和诊断价值。方法采用RT-PCR技术检测62例肾细胞癌患者肿瘤组织和外周血液中MN/CA IX mRNA的表达,以32例正常肾组织和非肾癌患者外周血作为对照。采用免疫组化技术检测36例肾透明细胞癌,17例其它肾肿瘤,16例正常肾组织标本。结果 RT-PCR结果显示62例肾细胞癌患者MN/CAⅨmRNA阳性率在癌组织中为82.3%(51/62),在外周血中为54.8%(34/62),显著高于对照组(P<0.05),在肾透明细胞癌患者癌组织和外周血中MN/CAⅨmRNA阳性率分别为98%(49/50)和66%(33/50),显著高于其它类型肾癌结果(P<0.05)。免疫组化结果显示MN/CAⅨ在肾透明细胞癌中表达阳性率为97.2%(35/36),明显高于其它类型肾肿瘤和正常肾组织(P<0.05)。结论 MN/CAⅨ在肾细胞癌尤其是透明细胞癌中有特异性的高表达,可以用于肾癌的诊断,尤其在预后和个体化治疗判断上有潜在价值。展开更多
Geochemical ratios in coral reef skeletons could be used as proxies to reconstruct past climatological and environmental records in data-poor regions. Using a 103-year data set(1902 to 2005), the annual variations in ...Geochemical ratios in coral reef skeletons could be used as proxies to reconstruct past climatological and environmental records in data-poor regions. Using a 103-year data set(1902 to 2005), the annual variations in Ba/Ca and Mn/Ca ratios of Porites lutea skeletons at an eastern offshore area of Hainan Island(19?12'28.4''N, 110?37'38.8''E) were analyzed using inductively coupled plasma-optic emission spectrometry(ICP-OES). The analysis results showed that Ba/Ca ratios varied from a minimum of 3.120 μmol mol^(-1) in 1903 to a maximum of 10.064 μmol mol^(-1) in 1944, with an average of 5.256 μmol mol^(-1). Mn/Ca ratios varied from 0.206 to 5.708 μmol mol^(-1) with an annual average of 1.234 μmol mol^(-1), with peak values in 2001, 1964 and 1932, that correlated with strong rainfall events caused by typhoons. Variation in Ba/Ca and Mn/Ca ratios were compared with available river discharge and precipitation records, providing insight into past climatological events. Human activities and their indirect effects could impact the strength of the relationship between Ba/Ca and Mn/Ca ratios and observed precipitation and terrestrial input in the future.展开更多
文摘目的探讨MN/CAⅨ在肾细胞癌组织和血液中的表达情况和诊断价值。方法采用RT-PCR技术检测62例肾细胞癌患者肿瘤组织和外周血液中MN/CA IX mRNA的表达,以32例正常肾组织和非肾癌患者外周血作为对照。采用免疫组化技术检测36例肾透明细胞癌,17例其它肾肿瘤,16例正常肾组织标本。结果 RT-PCR结果显示62例肾细胞癌患者MN/CAⅨmRNA阳性率在癌组织中为82.3%(51/62),在外周血中为54.8%(34/62),显著高于对照组(P<0.05),在肾透明细胞癌患者癌组织和外周血中MN/CAⅨmRNA阳性率分别为98%(49/50)和66%(33/50),显著高于其它类型肾癌结果(P<0.05)。免疫组化结果显示MN/CAⅨ在肾透明细胞癌中表达阳性率为97.2%(35/36),明显高于其它类型肾肿瘤和正常肾组织(P<0.05)。结论 MN/CAⅨ在肾细胞癌尤其是透明细胞癌中有特异性的高表达,可以用于肾癌的诊断,尤其在预后和个体化治疗判断上有潜在价值。
基金supported by the National Ocean Welfare Research Funds of State Ocean Administration (No.201105012)
文摘Geochemical ratios in coral reef skeletons could be used as proxies to reconstruct past climatological and environmental records in data-poor regions. Using a 103-year data set(1902 to 2005), the annual variations in Ba/Ca and Mn/Ca ratios of Porites lutea skeletons at an eastern offshore area of Hainan Island(19?12'28.4''N, 110?37'38.8''E) were analyzed using inductively coupled plasma-optic emission spectrometry(ICP-OES). The analysis results showed that Ba/Ca ratios varied from a minimum of 3.120 μmol mol^(-1) in 1903 to a maximum of 10.064 μmol mol^(-1) in 1944, with an average of 5.256 μmol mol^(-1). Mn/Ca ratios varied from 0.206 to 5.708 μmol mol^(-1) with an annual average of 1.234 μmol mol^(-1), with peak values in 2001, 1964 and 1932, that correlated with strong rainfall events caused by typhoons. Variation in Ba/Ca and Mn/Ca ratios were compared with available river discharge and precipitation records, providing insight into past climatological events. Human activities and their indirect effects could impact the strength of the relationship between Ba/Ca and Mn/Ca ratios and observed precipitation and terrestrial input in the future.
