[Objective] To analyze the components and structure of Opuntia milpa alta polysaccharides, and to study its anticoagulant activity. [Method] The crude polysaccharides （CP） were prepared from Opuntia milpa alta using...[Objective] To analyze the components and structure of Opuntia milpa alta polysaccharides, and to study its anticoagulant activity. [Method] The crude polysaccharides （CP） were prepared from Opuntia milpa alta using hot water extraction and ethanol precipitation method, purified by anion-exchange chromatography and gel filtration chromatography to study its monosaccharide composition, weight-average molecular weight distribution and main chain structure. And a preliminary study was conducted on the anticoagulant activity of polysaccharide components. [Result] The polysaccharide components WSP1, WSP2a and WSP3 were obtained from CP by the anion-exchange chromatography and gel filtration chromatography. WSP1 was mainly composed of galactose with a weight-average molecular weight of 2.32×106. WSP2a consisted of rhamnose, arabinose, xylose, mannose, glucose, galactose, glucuronic acid and galacturonic acid, the contents were 6.3%, 32.3%, 12.9%, 1.5%, 4.8%, 37.1%, 0.64% and 4.4%, respectively. WSP3 mainly composed of arabinose, xylose, rhamnose and trace of glucose, mannose, uronic acid. The weight-average molecular weight of WSP2a and WSP3 was 1.24×106 and 7.92×106, respectively. WSP2 prolonged the activated partial thromboplatin time （APTT） and thrombin time （TT）, while had no significant effect on the prothrombin time （PT） in vitro, indicating that anticoagulant activity of WSP2 functions by affecting the intrinsic coagulation system. The test of inhibition to the conversion of fibrinogen into thrombin showed that WSP2a and WSP3 had obvious anticoagulant activity. Methylation analysis indicated that the main chain of WSP2a was constituted of 1, 4-GalA, 1, 2-Rha and 1, 2, 4-Rha. [Conclusion] This study provided experimental basis for the optimization and development of polysaccharide resources from cactus展开更多
Opuntia Milpa Alta is a cactus cultivated,domesticated,hybridized and selected from the plant Opuntia ficusindica by Mexican agricultural experts,which can be used as fruit and vegetable.Opuntia Milpa Alta leaves and ...Opuntia Milpa Alta is a cactus cultivated,domesticated,hybridized and selected from the plant Opuntia ficusindica by Mexican agricultural experts,which can be used as fruit and vegetable.Opuntia Milpa Alta leaves and fruit are superior to wild varieties and suitable for storage and transportation.In 1998,Opuntia Milpa Alta was introduced to China from Mexico by the Quality Product Development Center of the Ministry of Agriculture of China.Up to now,the Opuntia Milpa Alta has been cultivated on a certain scale in China.This study aims to identify the research progress and development trends of Opuntia Milpa Alta in China.Papers published between 1998 to 2019 from two major Chinese academic databases(CNKI and Wangfang)with a topic search related to Opuntia Milpa Alta were collected.The research progress and development trends were analyzed based on CiteSpace software of text mining and visualization.The analysis found that Opuntia Milpa Alta has gone through three obvious research phases after being introduced to China.In the first phase,the researchers paid attention to its cultivation method.Subsequently,researchers began to use extraction methods to extract some of its components,such as polysaccharides and flavonoids.Finally,these extracted ingredients began to be used in some biomedical research.展开更多
对热水提取米邦塔仙人掌(Opuntia milpa alta Haw)粗多糖的提取工艺进行研究。以新鲜米邦塔仙人掌为原料,采用单因素试验考察料液比、提取温度、提取时间和提取次数对米邦塔仙人掌粗多糖得率的影响,并通过响应面法优化工艺参数。结果表...对热水提取米邦塔仙人掌(Opuntia milpa alta Haw)粗多糖的提取工艺进行研究。以新鲜米邦塔仙人掌为原料,采用单因素试验考察料液比、提取温度、提取时间和提取次数对米邦塔仙人掌粗多糖得率的影响,并通过响应面法优化工艺参数。结果表明,影响粗多糖得率的因素主次顺序为提取温度>料液比>提取时间。确定最佳提取工艺参数为提取温度83℃,料液比1∶28(g∶m L),提取时间为3 h,在此条件下,仙人掌粗多糖的得率为2.05%。展开更多
基金Supported by the National Natural Science Foundation of China(31173753)the International Scientific and Technological Cooperation Project of Anhui Province(10080703035)~~
文摘[Objective] To analyze the components and structure of Opuntia milpa alta polysaccharides, and to study its anticoagulant activity. [Method] The crude polysaccharides （CP） were prepared from Opuntia milpa alta using hot water extraction and ethanol precipitation method, purified by anion-exchange chromatography and gel filtration chromatography to study its monosaccharide composition, weight-average molecular weight distribution and main chain structure. And a preliminary study was conducted on the anticoagulant activity of polysaccharide components. [Result] The polysaccharide components WSP1, WSP2a and WSP3 were obtained from CP by the anion-exchange chromatography and gel filtration chromatography. WSP1 was mainly composed of galactose with a weight-average molecular weight of 2.32×106. WSP2a consisted of rhamnose, arabinose, xylose, mannose, glucose, galactose, glucuronic acid and galacturonic acid, the contents were 6.3%, 32.3%, 12.9%, 1.5%, 4.8%, 37.1%, 0.64% and 4.4%, respectively. WSP3 mainly composed of arabinose, xylose, rhamnose and trace of glucose, mannose, uronic acid. The weight-average molecular weight of WSP2a and WSP3 was 1.24×106 and 7.92×106, respectively. WSP2 prolonged the activated partial thromboplatin time （APTT） and thrombin time （TT）, while had no significant effect on the prothrombin time （PT） in vitro, indicating that anticoagulant activity of WSP2 functions by affecting the intrinsic coagulation system. The test of inhibition to the conversion of fibrinogen into thrombin showed that WSP2a and WSP3 had obvious anticoagulant activity. Methylation analysis indicated that the main chain of WSP2a was constituted of 1, 4-GalA, 1, 2-Rha and 1, 2, 4-Rha. [Conclusion] This study provided experimental basis for the optimization and development of polysaccharide resources from cactus
基金supported by the National Natural Science Foundation of China(22004026)Guangdong Provincial Department of Education Young Innovative Talents Project(2019KQNCX179)North Jiangsu Science and Technology Special Project(SZ-SQ2017006).
文摘Opuntia Milpa Alta is a cactus cultivated,domesticated,hybridized and selected from the plant Opuntia ficusindica by Mexican agricultural experts,which can be used as fruit and vegetable.Opuntia Milpa Alta leaves and fruit are superior to wild varieties and suitable for storage and transportation.In 1998,Opuntia Milpa Alta was introduced to China from Mexico by the Quality Product Development Center of the Ministry of Agriculture of China.Up to now,the Opuntia Milpa Alta has been cultivated on a certain scale in China.This study aims to identify the research progress and development trends of Opuntia Milpa Alta in China.Papers published between 1998 to 2019 from two major Chinese academic databases(CNKI and Wangfang)with a topic search related to Opuntia Milpa Alta were collected.The research progress and development trends were analyzed based on CiteSpace software of text mining and visualization.The analysis found that Opuntia Milpa Alta has gone through three obvious research phases after being introduced to China.In the first phase,the researchers paid attention to its cultivation method.Subsequently,researchers began to use extraction methods to extract some of its components,such as polysaccharides and flavonoids.Finally,these extracted ingredients began to be used in some biomedical research.
