In wave-equation migration and demigration,the cross-correlation imaging/forwarding step implicitly injects an additional copy of the source wavelet,so that the amplitude spectrum of the wavelet is applied redundantly...In wave-equation migration and demigration,the cross-correlation imaging/forwarding step implicitly injects an additional copy of the source wavelet,so that the amplitude spectrum of the wavelet is applied redundantly(effectively imposing a wavelet-spectrum weighting,often akin to an amplitude-squared bias).This redundancy degrades structural fidelity and amplitude balance yet is frequently overlooked.We(i)formalize the mechanism by which cross-correlation duplicates the source-wavelet amplitude effect in both migration and demigration,and(ii)introduce a source-equalized operator that removes the redundancy by deconvolving(or dividing by)the wavelet amplitude spectrum in the imaging condition and its demigration counterpart,while leaving phase/kinematics intact.Using a band-limited Ricker wavelet on a two-layer model and on Marmousi,we show that,if unmanaged,the redundant wavelet spectrum broadens main lobes,introduces ringing,and suppresses vertical resolution in migrated images,and inflates spectrum mismatches between demigrated and observed data even when peak times agree.With our correction,images recover observed-data-consistent bandwidth and sharpened interfaces,and demigrated data also exhibit improved spectrum conformity and reduced amplitude misfit.The results clarify when source amplitudes matter,why cross-correlation makes them redundantly matter,and how a lightweight spectral correction restores physically meaningful amplitude behavior in wave-equation migration/demigration.展开更多
The published article titled“MicroRNA-133b Inhibits Proliferation,Cellular Migration,and Invasion via Targeting LASP1 in Hepatocarcinoma Cells”has been retracted from Oncology Research,Vol.25,No.8,2017,pp.1269–1282.
Adult neurogenesis continuously produces new neurons critical for cognitive plasticity in adult rodents.While it is known transforming growth factor-βsignaling is important in embryonic neurogenesis,its role in postn...Adult neurogenesis continuously produces new neurons critical for cognitive plasticity in adult rodents.While it is known transforming growth factor-βsignaling is important in embryonic neurogenesis,its role in postnatal neurogenesis remains unclear.In this study,to define the precise role of transforming growth factor-βsignaling in postnatal neurogenesis at distinct stages of the neurogenic cascade both in vitro and in vivo,we developed two novel inducible and cell type-specific mouse models to specifically silence transforming growth factor-βsignaling in neural stem cells in(mGFAPcre-ALK5fl/fl-Ai9)or immature neuroblasts in(DCXcreERT2-ALK5fl/fl-Ai9).Our data showed that exogenous transforming growth factor-βtreatment led to inhibition of the proliferation of primary neural stem cells while stimulating their migration.These effects were abolished in activin-like kinase 5(ALK5)knockout primary neural stem cells.Consistent with this,inhibition of transforming growth factor-βsignaling with SB-431542 in wild-type neural stem cells stimulated proliferation while inhibited the migration of neural stem cells.Interestingly,deletion of transforming growth factor-βreceptor in neural stem cells in vivo inhibited the migration of postnatal born neurons in mGFAPcre-ALK5fl/fl-Ai9 mice,while abolishment of transforming growth factor-βsignaling in immature neuroblasts in DCXcreERT2-ALK5fl/fl-Ai9 mice did not affect the migration of these cells in the hippocampus.In summary,our data supports a dual role of transforming growth factor-βsignaling in the proliferation and migration of neural stem cells in vitro.Moreover,our data provides novel insights on cell type-specific-dependent requirements of transforming growth factor-βsignaling on neural stem cell proliferation and migration in vivo.展开更多
Breast cancer(BRCA)is characterized by high heterogeneity,with aggressive subtypes frequently showing poor prognosis and resistance to conventional therapies,making the discovery of new therapeutic targets and strateg...Breast cancer(BRCA)is characterized by high heterogeneity,with aggressive subtypes frequently showing poor prognosis and resistance to conventional therapies,making the discovery of new therapeutic targets and strategies imperative.Although elevated expression of discs large homolog 3(DLG3)has been reported in BRCA,its functional role in disease progression remains unclear.We performed bioinformatic analyses of clinical datasets to evaluate the prognostic significance of DLG3 expression in BRCA patients.In vitro gain-and loss-of-function experiments were conducted to assess the impact of DLG3 on BRCA cell proliferation,migration,and colony formation.Transcriptomic profiling,coupled with pharmacological inhibition,was employed to identify and validate downstream signaling pathways.Additionally,we extended our validation to an in vivo model to assess the role of DLG3 in tumor progression.We found that elevated DLG3 levels correlated with poor prognosis in breast cancer patients.Functionally,DLG3 overexpression significantly promoted cell proliferation and migration in estrogen receptor-positive MCF7 and triple-negative MDA-MB-231 breast cancer cells,whereas its knockdown suppressed these effects.Transcriptomic analyses revealed that DLG3 activates signal transducer and activator of transcription 3(STAT3)signaling,a finding further corroborated by Western blot.Critically,treatment with the STAT3 inhibitor Stattic attenuated DLG3-driven proliferation and migration,supporting a DLG3-STAT3 oncogenic axis.Furthermore,in vivo studies validated the role of DLG3 in promoting tumor growth and its correlation with elevated STAT3 signaling,consistent with our in vitro findings.Our findings establish DLG3 as a novel driver of breast cancer progression that directly activates STAT3 signaling.DLG3 thus represents both a potential prognostic biomarker and a promising therapeutic target for aggressive breast cancer subtypes,including triple-negative breast cancer.展开更多
Bacterial cells are widely accepted as nucleation sites for calcium carbonate precipitation in biomineralization based on the Microbially Induced Carbonate Precipitation(MICP)process.For MICP-based insitu biotreatment...Bacterial cells are widely accepted as nucleation sites for calcium carbonate precipitation in biomineralization based on the Microbially Induced Carbonate Precipitation(MICP)process.For MICP-based insitu biotreatment,the firstproblem to be solved is how to introduce and retain the bacterial cells in the soil,which involves the migration and retention of bacterial cells during the biogrouting process.Soil particle size,a key factor in determining pore throat size,can have a significanteffect on the migration and retention of bacterial cells in the soil and therefore on biomineralization.To investigate the effect of particle size on the migration and retention of bacterial cells in sand and its biomineralization,two sets of tests were carried out in this study,including percolation tests and sand column treatment tests.Soil urease activity(definedas urease activity per unit mass of soil)and calcium carbonate content of the biomineralized sand were measured to comprehensively assess the migration and retention of bacterial cells in the sand.The results indicate that sands with a particle size smaller than 0.25 mmwould inhibit the migration of bacteria in the sand,resulting in a nonuniform distribution of precipitated calcium carbonate and a low strength enhancement of biomineralization.On the other hand,sands with a particle size larger than 1.18 mm are unfavorable for retaining bacterial cells in the sand,resulting in low calcium conversion efficiency.Meanwhile,particle size would also affect the formation of effective calcium carbonate through interparticle contact number and interparticle pore size,and thus biomineralization.展开更多
Oncology Research Editorial Office Published:23 March 2026 The published article titled“TRAF4 Regulates Migration,Invasion,and Epithelial-Mesenchymal Transition via PI3K/AKT Signaling in Hepatocellular Carcinoma”has...Oncology Research Editorial Office Published:23 March 2026 The published article titled“TRAF4 Regulates Migration,Invasion,and Epithelial-Mesenchymal Transition via PI3K/AKT Signaling in Hepatocellular Carcinoma”has been retracted from Oncology Research,Vol.25,No.8,2017,pp.1329-1340.DOI:10.3727/096504017X14876227286564 URL:https://www.techscience.com/or/v25n8/56917.展开更多
Sodium layered oxides stand out as one of the most promising cathodes for sodium-ion batteries due to their high energy density,elemental abundance,and scalability.However,their practical applications are restricted b...Sodium layered oxides stand out as one of the most promising cathodes for sodium-ion batteries due to their high energy density,elemental abundance,and scalability.However,their practical applications are restricted by interplanar gliding,cation migration,and the formation of intragranular microcracks,which collectively lead to rapid structural degradation and capacity loss.Herein,we rationally design an ultrastable O3-type Na_(0.94)Ca_(0.03)Ni_(1/3)Fe_(1/3)Mn_(1/3)O_(2) cathode,in which Ca^(2+)cations act as pillars within the NaO_(2)slabs,suppressing the irreversible phase transitions and Na/TM cation migration commonly observed in layered oxides.Multiscale in situ and ex situ techniques,combined with post-mortem analysis,reveal that the Ca-pillared pinning effect not only effectively suppresses the interplanar gliding and stress accumulation within the crystal phase but also restrains Na/TM cation migration and surface reconstruction in near-surface regions.Benefiting from the combined effects of structural stabilization,the Ca-pillared cathode exhibits a superior cycling stability,retaining 81.6%of its capacity after 1000 cycles at 2 C within the voltage range of 2.0-4.0 V,along with significantly enhanced wide-temperature(from-40 to 80℃)performance.This work highlights another critical role of Ca pillars in suppressing cation migration and surface structural degradation beyond preventing adverse interplanar gliding,offering valuable insights for designing long-life and wide-temperature layered oxide cathodes.展开更多
High-performance intelligent protective materials are vital for nuclear energy systems exposed to extreme irradiation.Among them,tungsten-based alloys demonstrate exceptional potential owing to their superior irradiat...High-performance intelligent protective materials are vital for nuclear energy systems exposed to extreme irradiation.Among them,tungsten-based alloys demonstrate exceptional potential owing to their superior irradiation resistance.Recent experimental studies have demonstrated that W-Ta-Cr-V alloys exhibit excellent irradiation resistance under helium(He)ion irradiation.However,the underlying mechanisms,especially the migration behavior of He atoms,remain unclear.In this work,the influences of uniaxial tensile and compressive strain on He migration in W-Ta-Cr-V complex alloys have been systematically studied through first-principles calculations.Our results demonstrate that He atoms preferentially occupy the tetrahedral interstitial sites,with interstitial formation energies significantly reduced compared to pure W.The introduction of Ta,Cr,and V alloying elements markedly increases the He migration barriers,effectively suppressing He diffusion.Compressive strain increases the migration barriers,inhibiting He bubbles nucleation and growth,while tensile strain decreases the barriers,facilitating bubble formation.Compared to pure W,the W-Ta-Cr-V alloys exhibit both lower He interstitial formation energies and higher migration barriers,with further enhancement under compressive strain.Specifically,compressive strain of 6%increases the He migration barrier of the W-Ta-Cr-V alloy by 0.166 e V,which further widens the difference relative to pure W.These findings provide a theoretical explanation for the superior irradiation resistance of tungsten-based alloys observed experimentally and promote the understanding of irradiation damage in these alloys under strain.展开更多
Regulating the critical process of proton migration from water dissociation for boosting alkaline hydrogen evolution reaction(HER)remains a challenge.Herein,we propose an electrostatic attraction strategy to achieve t...Regulating the critical process of proton migration from water dissociation for boosting alkaline hydrogen evolution reaction(HER)remains a challenge.Herein,we propose an electrostatic attraction strategy to achieve the migration of a highly efficient hydrogen species to Pt sites over Pt/Co@NC,which is obtained through a facile calcination and electrodeposition method.It exhibits an outstanding geometric activity(η_(10)=31 m V),which surpasses the commercial 20 wt%Pt/C(η_(10)=37 mV).Moreover,the mass activity of Pt/Co@NC is 5.6 A mg_(Pt)^(-1) at-50 mV vs.RHE,which is 2.23 times higher than that of 20 wt%Pt/C.Experimental and theoretical results indicate that the work function of the outer carbon layer,which is changed by the introduction of the inner cobalt core,plays a crucial role in reversing the direction of electron migration between the carbon layer and Pt.The negatively charged Pt^(δ-)can spontaneously attract positively charged protons via the electrostatic interaction effect,thereby achieving the directional migration of hydrogen species.This work presents a strategy for designing advanced alkaline HER electrocatalysts by the electrostatic effect.展开更多
As the primary functional component of a fusion reactor,the fusion blanket pebble bed,composed of numerous particles,is crucial for tritium breeding,neutron multiplication,and radiation shielding.Particles within trit...As the primary functional component of a fusion reactor,the fusion blanket pebble bed,composed of numerous particles,is crucial for tritium breeding,neutron multiplication,and radiation shielding.Particles within tritium-breeding pebble beds are subjected to prolonged neutron irradiation,high thermal loads,and strong magnetic fields in fusion environments.Such conditions render them susceptible to pulverization and fragmentation.The resulting fragments and powders migrate and are deposited into the gas channel,driven by the purge gas.The reduction in the effective flow area of the gas increases the flow resistance,resulting in tritium retention,degraded heat transfer,and other adverse effects.These conditions impair the thermodynamic properties of the pebble beds and hinder the self-maintenance of tritium.Limited information exists on powder migration and clogging mechanisms in fusion blanket pebble beds,particularly under diverse physical conditions.The aim of this study was to use a computational fluid dynamics model coupled with the discrete element method(CFD-DEM)to numerically explore powder migration and clogging in pebble beds.The model considers factors such as breeder orientation,purge velocity,powder size distribution,and friction coefficient.We propose two migration and clogging mechanisms.One involves powder with a large particle size,and the other does not.The results indicate that the powder migration velocity progresses through three stages:rapid decay,linear decay,and stability.Pebble-bed clogging manifests in two forms:extensive superficial clogging and uniform internal clogging.Two fitted curves were used to depict the migration and clogging tendencies.The powder size distribution significantly influenced the powder migration.The breeder orientation,powder size,and friction coefficient affected the distribution of the clogging powders.However,the impact of the purge velocity on powder migration and clogging in pebble beds was limited,and this effect varied significantly with different particle size ratios.Based on the analysis,a formula is proposed to characterize the behavior of the powder in the pebble beds.The results of this study can aid in analyzing and predicting powder dynamics in pebble beds.展开更多
Phosphorus tends to migrate into metallic iron during the direct reduction of high-phosphorus oolitic iron ore,leading to undesirable phosphorus enrichment in metallic iron.However,the underlying reduction and migrati...Phosphorus tends to migrate into metallic iron during the direct reduction of high-phosphorus oolitic iron ore,leading to undesirable phosphorus enrichment in metallic iron.However,the underlying reduction and migration mechanisms remain poorly understood.Phosphorus behavior during coal-based reduction was systematically investigated through theoretical modeling and experimental approaches.Thermodynamic analysis revealed that the carbon reduction of solid Ca_(3)(PO_(4))_(2)to gaseous P_(2)requires temperatures exceeding 1400℃.Notably,this threshold significantly decreases to 1130.5℃in the presence of SiO_(2)and Al_(2)O_(3).Further investigations demonstrated that Ca_(3)(PO_(4))_(2)co-reduces with Fe_(x)O_(γ)in the presence of SiO_(2)-Al_(2)O_(3)-Fe_(x)O_(γ),forming Fe_(3)P(instead of gaseous P_(2))at a markedly lower temperature of 778.7℃.Mechanistic studies indicate that the inherent thermal stability of Ca_(3)(PO_(4))_(2)inhibits the generation of reactive[P_(2)O_(5)].However,SiO_(2)-Al_(2)O_(3)coexistence destabilizes Ca_(3)(PO_(4))_(2)while exponentially enhancing[P_(2)O_(5)]activity.This synergistic effect dramatically promotes the phosphorus mineral reduction.Characterization confirmed that Ca_(3)(PO_(4))_(2)migrated into the slag phase(4FeO·Al_(2)O_(3)·3SiO_(2)·CaO·P_(2)O_(5)).Subsequently,the reactive P_(2)O_(5)in slag is reduced with metallic iron to form Fe_(3)P,which further dissolves into theα-Fe matrix through solid-state diffusion,ultimately generating Fe-P solid solutions.展开更多
With the increasing use of passive seismic data,developing seismic reflection imaging methods based on passive data is of considerable practical significance.This study presents a waveform-matching reverse time migrat...With the increasing use of passive seismic data,developing seismic reflection imaging methods based on passive data is of considerable practical significance.This study presents a waveform-matching reverse time migration for the primary reflected data from local earthquakes.In order to mitigate inconsistencies in frequency band and energy across earthquakes of different magnitudes,we first establish reference seismic waveform with standardized dominant frequency and magnitude.A matching operator is derived for each event by matching its waveforms with the reference waveform.This operator is then applied via convolution to all waveforms,producing standardized seismic waveforms with consistent wavelet features.The reshaped waveforms are then subjected to reverse time migration using an impedance imaging condition for primary reflections.To suppress strong energy interference near the hypocenters,both illumination compensation and three-dimensional Smoothed Spherical Mask centered on each source are used.Numerical tests using both simple two-layer model and fault-containing model demonstrate that the new method is robust and effective.The reverse time migration of primary reflected data of local earthquakes accurately images underground impedance boundaries such as stratum interfaces and fault planes,showing its promise for future application in seismically active fault zones.展开更多
Peripheral nerve defect repair is a complex process that involves multiple cell types;perineurial cells play a pivotal role.Hair follicle neural crest stem cells promote perineurial cell proliferation and migration vi...Peripheral nerve defect repair is a complex process that involves multiple cell types;perineurial cells play a pivotal role.Hair follicle neural crest stem cells promote perineurial cell proliferation and migration via paracrine signaling;however,their clinical applications are limited by potential risks such as tumorigenesis and xenogeneic immune rejection,which are similar to the risks associated with other stem cell transplantations.The present study therefore focuses on small extracellular vesicles derived from hair follicle neural crest stem cells,which preserve the bioactive properties of the parent cells while avoiding the transplantation-associated risks.In vitro,small extracellular vesicles derived from hair follicle neural crest stem cells significantly enhanced the proliferation,migration,tube formation,and barrier function of perineurial cells,and subsequently upregulated the expression of tight junction proteins.Furthermore,in a rat model of sciatic nerve defects bridged with silicon tubes,treatment with small extracellular vesicles derived from hair follicle neural crest stem cells resulted in higher tight junction protein expression in perineurial cells,thus facilitating neural tissue regeneration.At 10 weeks post-surgery,rats treated with small extracellular vesicles derived from hair follicle neural crest stem cells exhibited improved nerve function recovery and reduced muscle atrophy.Transcriptomic and micro RNA analyses revealed that small extracellular vesicles derived from hair follicle neural crest stem cells deliver mi R-21-5p,which inhibits mothers against decapentaplegic homolog 7 expression,thereby activating the transforming growth factor-β/mothers against decapentaplegic homolog signaling pathway and upregulating hyaluronan synthase 2 expression,and further enhancing tight junction protein expression.Together,our findings indicate that small extracellular vesicles derived from hair follicle neural crest stem cells promote the proliferation,migration,and tight junction protein formation of perineurial cells.These results provide new insights into peripheral nerve regeneration from the perspective of perineurial cells,and present a novel approach for the clinical treatment of peripheral nerve defects.展开更多
The authors regret to report the following error made in“Spatiotemporal dynamics of neuron differentiation and migration in the developing human spinal cord;52(2025)-101283-1295;Doi:https://doi.org/10.1016/j.jgg.2025...The authors regret to report the following error made in“Spatiotemporal dynamics of neuron differentiation and migration in the developing human spinal cord;52(2025)-101283-1295;Doi:https://doi.org/10.1016/j.jgg.2025.08.004”.In Tables S1 and S2 in the supplementary materials of this paper,some items were written in Chinese.The corresponding pictures and tables were not uploaded in time.展开更多
Ecological barriers present significant challenges to bird migration by limiting the availability of stopover sites and shelters. The Qinghai-Tibet Plateau, a major migratory barrier located in higher latitude Central...Ecological barriers present significant challenges to bird migration by limiting the availability of stopover sites and shelters. The Qinghai-Tibet Plateau, a major migratory barrier located in higher latitude Central Asia, exerts a substantial influence on avian migration patterns. Species traversing such ecological barriers may adopt multiple optimal routes, which can contribute to the formation of migratory divides. From 2018 to 2021, the migration routes of 13 adult Common Cuckoos (Cuculus canorus) breeding in the north of the Qinghai-Tibet Plateau were tracked using satellite transmitters. We found Common Cuckoos have two primary migration routes: western and eastern, respectively following western and eastern edges of the Qinghai-Tibet plateau. The eastern and western routes are likely the optimal routes for the Central Asian Common Cuckoos population to navigate the Qinghai-Tibet Plateau. Furthermore, two individuals exhibited intermediate migration routes, suggesting attempted traverses of the Qinghai-Tibet Plateau, although neither completed the migration. These intermediate routes may indicate migratory behavior influenced by hybridization between eastern and western populations or migratory flexibility. Common Cuckoos exhibit significantly faster migration speed, flight speed, and shorter stopover durations during spring compared to autumn. The observed seasonal differences in migration behavior support birds following time-minimization strategies during spring migration. These results revealed the diverse migration routes of Common Cuckoos shaped by the Qinghai-Tibet Plateau and seasonal variation in migration patterns.展开更多
This paper aims to elucidate the seismic characteristics of the Three Gorges Reservoir area after impoundment and investigate the seismic source migration.Based on the seismic data analysis from the Badong segment in ...This paper aims to elucidate the seismic characteristics of the Three Gorges Reservoir area after impoundment and investigate the seismic source migration.Based on the seismic data analysis from the Badong segment in the Three Gorges Reservoir area,we assessed the local temporal and spatial variations in the frequent earthquakes.Correlation analysis was conducted to investigate the relationship between changes in reservoir water levels and the occurrence of reservoir-induced earthquakes.Additionally,we examined the regularity of earthquake occurrences at the exact location during different periods.Based on the fault mechanics principles,a formula was derived to estimate the length of open and wing-shaped rupture at the hypocenter under the influence of pore or excess pore water pressure.The results reveal that reservoir-induced seismicity demonstrates short-term cycles characterized by alternating"active periods"and"quiet periods,"as well as long-term cycles with the combined periods.The probability of earthquakes occurring within one year at the epicentre is relatively high and decreases after four years.The derived formula can be utilized to estimate the seismic migration distance at the epicentre in the short term.These research findings provide valuable insights for analyzing the regularity of reservoir-induced earthquake activities and understanding the mechanism of seismic source migration.展开更多
The gas-liquid countercurrent flow pattern is complex and the bubble migration velocity is difficult to predict in the process of bullheading well killing.The experiment on bubble migration in gas-liquid countercurren...The gas-liquid countercurrent flow pattern is complex and the bubble migration velocity is difficult to predict in the process of bullheading well killing.The experiment on bubble migration in gas-liquid countercurrent flow in annulus is carried out under different working conditions to reveal how the wellbore inclination angle,liquid phase property and countercurrent liquid velocity affect the bubble deformation and bubble migration trajectory/velocity,and to establish a bubble migration velocity prediction model.The bubbles in the countercurrent flow mainly migrate in two modes:free rising of isolated bubbles,and interactive rising of multiple bubbles.The bubbles migrate by an S-shaped trajectory in the countercurrent flow.With the increase of countercurrent liquid velocity,the lateral oscillation of bubbles is intensified.The increases of wellbore inclination angle,liquid density and liquid viscosity make the bubble migration trajectory gradually to be linear.The bubble is generally ellipsoidal during its rising.The wellbore inclination angle has little effect on the degree of bubble deformation.The bubbles are ellipsoidal during rising,with little influence of wellbore inclination angle on bubble deformation.With the increase of liquid viscosity and density,the aspect ratio of the bubble decreases.As the wellbore inclination angle increases,the bubble migration velocity gradually decreases.As the liquid viscosity increases,the bubble migration velocity decreases.As the liquid density increases,the bubble migration velocity increases slightly.The established bubble migration velocity prediction model yields errors within±15%,and demonstrates broad applicability across a wide range of operating conditions.展开更多
We propose a combined migration velocity analysis and imaging method based on Kirchhoff integral migration and reverse time migration,using the residual curvature analysis and layer stripping strategy to build the vel...We propose a combined migration velocity analysis and imaging method based on Kirchhoff integral migration and reverse time migration,using the residual curvature analysis and layer stripping strategy to build the velocity model.This method improves the image resolution of Kirchhoff integral migration and reduces the computations of the reverse time migration.It combines the advantages of efficiency and accuracy of the two migration methods.Its application in tunnel seismic prediction shows good results.Numerical experiments show that the imaging results of reverse time migration are better than the imaging results of Kirchhoff integral migration in many aspects of tunnel prediction.Field data show that this method has efficient computations and can establish a reasonable velocity model and a high quality imaging section.Combination with geological information can make an accurate prediction of the front of the tunnel geological structure.展开更多
Objective:Ovarian cancer(OC)ranks among the leading causes of mortality among the female cancers worldwide.Numerous studies have explored the development and progression of OC at multiple genetic regulatory levels.How...Objective:Ovarian cancer(OC)ranks among the leading causes of mortality among the female cancers worldwide.Numerous studies have explored the development and progression of OC at multiple genetic regulatory levels.However,relatively few studies have explored the impact of post-translational modifications(PTM)on OC progression,which is essential for uncovering new therapeutic targets.This study aimed to systematically identify the key PTM types involved in OCprogression,and to explore and evaluate their translational potential as therapeutic targets.Methods:First,we utilized multiple general PTM antibodies to compare gross PTM levels between normal ovarian and OC tissues from clinical females.After identifying lactylation as the PTM with the most significant differences,we selected representative samples for label-free mass spectrometry to identify specific lactylation sites.Next,we transfected A2780(OC)cells with either wild-type(WT)or mutant(K192A[Q])poly(ADP-ribose)polymerase 1(PARP1)conjugated to enhanced green fluorescent protein(EGFP)with a StrepⅡpeptide tag and assessed various cellular indexes related to cell proliferation(clonogenicity assay),migration(scratch wound healing assay),and reactive oxygen species levels.Results:Pan-lactylation was significantly upregulated in clinical OC samples,with PARP1 lactylation at K192 being one of the most common modifications.The growth and migration of A2780 cells were markedly suppressed by overexpressing PARP1-WT but not mutant PARP1.Overexpressing PARP1 significantly downregulated the phosphorylation of extracellular signal-regulated kinases 1/2(ERK1/2).Conclusion:This study uncovered a novel PTM of PARP1 in OC,lactylation,and demonstrated that lactylation at K192 is crucial in regulating OC cell growth and migration via the ERK1/2 pathway.Further investigations are required to elucidate the broader functional implications of PARP1 lactylation and its therapeutic potential.展开更多
基金supported by the National Natural Science Foundation of China(42430303)Strategy Priority Research Program(Category B)of the Chinese Academy of Sciences(XDB0710000)+2 种基金National Natural Science Foundation of China(42288201)the National Key R&D Program of China(2023YFF0803203)the IGGCAS start-up funding(Grant No.E251510101).
文摘In wave-equation migration and demigration,the cross-correlation imaging/forwarding step implicitly injects an additional copy of the source wavelet,so that the amplitude spectrum of the wavelet is applied redundantly(effectively imposing a wavelet-spectrum weighting,often akin to an amplitude-squared bias).This redundancy degrades structural fidelity and amplitude balance yet is frequently overlooked.We(i)formalize the mechanism by which cross-correlation duplicates the source-wavelet amplitude effect in both migration and demigration,and(ii)introduce a source-equalized operator that removes the redundancy by deconvolving(or dividing by)the wavelet amplitude spectrum in the imaging condition and its demigration counterpart,while leaving phase/kinematics intact.Using a band-limited Ricker wavelet on a two-layer model and on Marmousi,we show that,if unmanaged,the redundant wavelet spectrum broadens main lobes,introduces ringing,and suppresses vertical resolution in migrated images,and inflates spectrum mismatches between demigrated and observed data even when peak times agree.With our correction,images recover observed-data-consistent bandwidth and sharpened interfaces,and demigrated data also exhibit improved spectrum conformity and reduced amplitude misfit.The results clarify when source amplitudes matter,why cross-correlation makes them redundantly matter,and how a lightweight spectral correction restores physically meaningful amplitude behavior in wave-equation migration/demigration.
文摘The published article titled“MicroRNA-133b Inhibits Proliferation,Cellular Migration,and Invasion via Targeting LASP1 in Hepatocarcinoma Cells”has been retracted from Oncology Research,Vol.25,No.8,2017,pp.1269–1282.
基金supported by NIH grants,Nos.R01NS125074,R01AG083164,R01NS107365,and R21NS127177(to YL),1F31NS129204-01A1(to KW)and Albert Ryan Fellowship(to KW).
文摘Adult neurogenesis continuously produces new neurons critical for cognitive plasticity in adult rodents.While it is known transforming growth factor-βsignaling is important in embryonic neurogenesis,its role in postnatal neurogenesis remains unclear.In this study,to define the precise role of transforming growth factor-βsignaling in postnatal neurogenesis at distinct stages of the neurogenic cascade both in vitro and in vivo,we developed two novel inducible and cell type-specific mouse models to specifically silence transforming growth factor-βsignaling in neural stem cells in(mGFAPcre-ALK5fl/fl-Ai9)or immature neuroblasts in(DCXcreERT2-ALK5fl/fl-Ai9).Our data showed that exogenous transforming growth factor-βtreatment led to inhibition of the proliferation of primary neural stem cells while stimulating their migration.These effects were abolished in activin-like kinase 5(ALK5)knockout primary neural stem cells.Consistent with this,inhibition of transforming growth factor-βsignaling with SB-431542 in wild-type neural stem cells stimulated proliferation while inhibited the migration of neural stem cells.Interestingly,deletion of transforming growth factor-βreceptor in neural stem cells in vivo inhibited the migration of postnatal born neurons in mGFAPcre-ALK5fl/fl-Ai9 mice,while abolishment of transforming growth factor-βsignaling in immature neuroblasts in DCXcreERT2-ALK5fl/fl-Ai9 mice did not affect the migration of these cells in the hippocampus.In summary,our data supports a dual role of transforming growth factor-βsignaling in the proliferation and migration of neural stem cells in vitro.Moreover,our data provides novel insights on cell type-specific-dependent requirements of transforming growth factor-βsignaling on neural stem cell proliferation and migration in vivo.
文摘Breast cancer(BRCA)is characterized by high heterogeneity,with aggressive subtypes frequently showing poor prognosis and resistance to conventional therapies,making the discovery of new therapeutic targets and strategies imperative.Although elevated expression of discs large homolog 3(DLG3)has been reported in BRCA,its functional role in disease progression remains unclear.We performed bioinformatic analyses of clinical datasets to evaluate the prognostic significance of DLG3 expression in BRCA patients.In vitro gain-and loss-of-function experiments were conducted to assess the impact of DLG3 on BRCA cell proliferation,migration,and colony formation.Transcriptomic profiling,coupled with pharmacological inhibition,was employed to identify and validate downstream signaling pathways.Additionally,we extended our validation to an in vivo model to assess the role of DLG3 in tumor progression.We found that elevated DLG3 levels correlated with poor prognosis in breast cancer patients.Functionally,DLG3 overexpression significantly promoted cell proliferation and migration in estrogen receptor-positive MCF7 and triple-negative MDA-MB-231 breast cancer cells,whereas its knockdown suppressed these effects.Transcriptomic analyses revealed that DLG3 activates signal transducer and activator of transcription 3(STAT3)signaling,a finding further corroborated by Western blot.Critically,treatment with the STAT3 inhibitor Stattic attenuated DLG3-driven proliferation and migration,supporting a DLG3-STAT3 oncogenic axis.Furthermore,in vivo studies validated the role of DLG3 in promoting tumor growth and its correlation with elevated STAT3 signaling,consistent with our in vitro findings.Our findings establish DLG3 as a novel driver of breast cancer progression that directly activates STAT3 signaling.DLG3 thus represents both a potential prognostic biomarker and a promising therapeutic target for aggressive breast cancer subtypes,including triple-negative breast cancer.
基金support by the National Natural Science Foundation of China(NSFC)(Grant Nos.52178319,42477160,52338007).
文摘Bacterial cells are widely accepted as nucleation sites for calcium carbonate precipitation in biomineralization based on the Microbially Induced Carbonate Precipitation(MICP)process.For MICP-based insitu biotreatment,the firstproblem to be solved is how to introduce and retain the bacterial cells in the soil,which involves the migration and retention of bacterial cells during the biogrouting process.Soil particle size,a key factor in determining pore throat size,can have a significanteffect on the migration and retention of bacterial cells in the soil and therefore on biomineralization.To investigate the effect of particle size on the migration and retention of bacterial cells in sand and its biomineralization,two sets of tests were carried out in this study,including percolation tests and sand column treatment tests.Soil urease activity(definedas urease activity per unit mass of soil)and calcium carbonate content of the biomineralized sand were measured to comprehensively assess the migration and retention of bacterial cells in the sand.The results indicate that sands with a particle size smaller than 0.25 mmwould inhibit the migration of bacteria in the sand,resulting in a nonuniform distribution of precipitated calcium carbonate and a low strength enhancement of biomineralization.On the other hand,sands with a particle size larger than 1.18 mm are unfavorable for retaining bacterial cells in the sand,resulting in low calcium conversion efficiency.Meanwhile,particle size would also affect the formation of effective calcium carbonate through interparticle contact number and interparticle pore size,and thus biomineralization.
文摘Oncology Research Editorial Office Published:23 March 2026 The published article titled“TRAF4 Regulates Migration,Invasion,and Epithelial-Mesenchymal Transition via PI3K/AKT Signaling in Hepatocellular Carcinoma”has been retracted from Oncology Research,Vol.25,No.8,2017,pp.1329-1340.DOI:10.3727/096504017X14876227286564 URL:https://www.techscience.com/or/v25n8/56917.
基金supported by the National Key R&D Program of China(2023YFB2406000)the National Natural Science Foundation of China(22479057,52172201,51732005)。
文摘Sodium layered oxides stand out as one of the most promising cathodes for sodium-ion batteries due to their high energy density,elemental abundance,and scalability.However,their practical applications are restricted by interplanar gliding,cation migration,and the formation of intragranular microcracks,which collectively lead to rapid structural degradation and capacity loss.Herein,we rationally design an ultrastable O3-type Na_(0.94)Ca_(0.03)Ni_(1/3)Fe_(1/3)Mn_(1/3)O_(2) cathode,in which Ca^(2+)cations act as pillars within the NaO_(2)slabs,suppressing the irreversible phase transitions and Na/TM cation migration commonly observed in layered oxides.Multiscale in situ and ex situ techniques,combined with post-mortem analysis,reveal that the Ca-pillared pinning effect not only effectively suppresses the interplanar gliding and stress accumulation within the crystal phase but also restrains Na/TM cation migration and surface reconstruction in near-surface regions.Benefiting from the combined effects of structural stabilization,the Ca-pillared cathode exhibits a superior cycling stability,retaining 81.6%of its capacity after 1000 cycles at 2 C within the voltage range of 2.0-4.0 V,along with significantly enhanced wide-temperature(from-40 to 80℃)performance.This work highlights another critical role of Ca pillars in suppressing cation migration and surface structural degradation beyond preventing adverse interplanar gliding,offering valuable insights for designing long-life and wide-temperature layered oxide cathodes.
基金Project supported by the National Natural Science Foundation of China(Grant Nos.11505003 and 52325103)the Anhui Provincial Natural Science Foundation(Grant No.2108085MA25)。
文摘High-performance intelligent protective materials are vital for nuclear energy systems exposed to extreme irradiation.Among them,tungsten-based alloys demonstrate exceptional potential owing to their superior irradiation resistance.Recent experimental studies have demonstrated that W-Ta-Cr-V alloys exhibit excellent irradiation resistance under helium(He)ion irradiation.However,the underlying mechanisms,especially the migration behavior of He atoms,remain unclear.In this work,the influences of uniaxial tensile and compressive strain on He migration in W-Ta-Cr-V complex alloys have been systematically studied through first-principles calculations.Our results demonstrate that He atoms preferentially occupy the tetrahedral interstitial sites,with interstitial formation energies significantly reduced compared to pure W.The introduction of Ta,Cr,and V alloying elements markedly increases the He migration barriers,effectively suppressing He diffusion.Compressive strain increases the migration barriers,inhibiting He bubbles nucleation and growth,while tensile strain decreases the barriers,facilitating bubble formation.Compared to pure W,the W-Ta-Cr-V alloys exhibit both lower He interstitial formation energies and higher migration barriers,with further enhancement under compressive strain.Specifically,compressive strain of 6%increases the He migration barrier of the W-Ta-Cr-V alloy by 0.166 e V,which further widens the difference relative to pure W.These findings provide a theoretical explanation for the superior irradiation resistance of tungsten-based alloys observed experimentally and promote the understanding of irradiation damage in these alloys under strain.
基金financially supported by the Natural Science Foundation of the Jiangsu Higher Education Institutions of China(23KJB610003)the Natural Science Foundation of Jiangsu Province(BK20240339)+2 种基金the Science and Technology Support Plan for Youth Innovation of Colleges and Universities of Shandong Province of China(No.2023KJ104)the National Natural Science Foundation of China(No.52202092)the Natural Science Foundation of Shandong Province(No.ZR2022QE076)。
文摘Regulating the critical process of proton migration from water dissociation for boosting alkaline hydrogen evolution reaction(HER)remains a challenge.Herein,we propose an electrostatic attraction strategy to achieve the migration of a highly efficient hydrogen species to Pt sites over Pt/Co@NC,which is obtained through a facile calcination and electrodeposition method.It exhibits an outstanding geometric activity(η_(10)=31 m V),which surpasses the commercial 20 wt%Pt/C(η_(10)=37 mV).Moreover,the mass activity of Pt/Co@NC is 5.6 A mg_(Pt)^(-1) at-50 mV vs.RHE,which is 2.23 times higher than that of 20 wt%Pt/C.Experimental and theoretical results indicate that the work function of the outer carbon layer,which is changed by the introduction of the inner cobalt core,plays a crucial role in reversing the direction of electron migration between the carbon layer and Pt.The negatively charged Pt^(δ-)can spontaneously attract positively charged protons via the electrostatic interaction effect,thereby achieving the directional migration of hydrogen species.This work presents a strategy for designing advanced alkaline HER electrocatalysts by the electrostatic effect.
文摘As the primary functional component of a fusion reactor,the fusion blanket pebble bed,composed of numerous particles,is crucial for tritium breeding,neutron multiplication,and radiation shielding.Particles within tritium-breeding pebble beds are subjected to prolonged neutron irradiation,high thermal loads,and strong magnetic fields in fusion environments.Such conditions render them susceptible to pulverization and fragmentation.The resulting fragments and powders migrate and are deposited into the gas channel,driven by the purge gas.The reduction in the effective flow area of the gas increases the flow resistance,resulting in tritium retention,degraded heat transfer,and other adverse effects.These conditions impair the thermodynamic properties of the pebble beds and hinder the self-maintenance of tritium.Limited information exists on powder migration and clogging mechanisms in fusion blanket pebble beds,particularly under diverse physical conditions.The aim of this study was to use a computational fluid dynamics model coupled with the discrete element method(CFD-DEM)to numerically explore powder migration and clogging in pebble beds.The model considers factors such as breeder orientation,purge velocity,powder size distribution,and friction coefficient.We propose two migration and clogging mechanisms.One involves powder with a large particle size,and the other does not.The results indicate that the powder migration velocity progresses through three stages:rapid decay,linear decay,and stability.Pebble-bed clogging manifests in two forms:extensive superficial clogging and uniform internal clogging.Two fitted curves were used to depict the migration and clogging tendencies.The powder size distribution significantly influenced the powder migration.The breeder orientation,powder size,and friction coefficient affected the distribution of the clogging powders.However,the impact of the purge velocity on powder migration and clogging in pebble beds was limited,and this effect varied significantly with different particle size ratios.Based on the analysis,a formula is proposed to characterize the behavior of the powder in the pebble beds.The results of this study can aid in analyzing and predicting powder dynamics in pebble beds.
基金supported by the National Key Research and Development Program of China(Nos.2023YFC3903900 and 2023YFC3903904)the National Natural Science Foundation Youth Foundation of China(No.52404356)the China Baowu Low Carbon Metallurgy Innovation Foundation(No.BWLCF202216)。
文摘Phosphorus tends to migrate into metallic iron during the direct reduction of high-phosphorus oolitic iron ore,leading to undesirable phosphorus enrichment in metallic iron.However,the underlying reduction and migration mechanisms remain poorly understood.Phosphorus behavior during coal-based reduction was systematically investigated through theoretical modeling and experimental approaches.Thermodynamic analysis revealed that the carbon reduction of solid Ca_(3)(PO_(4))_(2)to gaseous P_(2)requires temperatures exceeding 1400℃.Notably,this threshold significantly decreases to 1130.5℃in the presence of SiO_(2)and Al_(2)O_(3).Further investigations demonstrated that Ca_(3)(PO_(4))_(2)co-reduces with Fe_(x)O_(γ)in the presence of SiO_(2)-Al_(2)O_(3)-Fe_(x)O_(γ),forming Fe_(3)P(instead of gaseous P_(2))at a markedly lower temperature of 778.7℃.Mechanistic studies indicate that the inherent thermal stability of Ca_(3)(PO_(4))_(2)inhibits the generation of reactive[P_(2)O_(5)].However,SiO_(2)-Al_(2)O_(3)coexistence destabilizes Ca_(3)(PO_(4))_(2)while exponentially enhancing[P_(2)O_(5)]activity.This synergistic effect dramatically promotes the phosphorus mineral reduction.Characterization confirmed that Ca_(3)(PO_(4))_(2)migrated into the slag phase(4FeO·Al_(2)O_(3)·3SiO_(2)·CaO·P_(2)O_(5)).Subsequently,the reactive P_(2)O_(5)in slag is reduced with metallic iron to form Fe_(3)P,which further dissolves into theα-Fe matrix through solid-state diffusion,ultimately generating Fe-P solid solutions.
基金supported by NIGMS(SC1GM140907 to G.-Y.L.)the Prostate Cancer Research Racial Disparity Grant from the Prostate Cancer Research(PCR)in the United Kingdom(grant reference[5001],to G.-Y.L.)the AU Medical Center,Inc.with a Grant/Contract Number CAU-AU Partnership-Kavuri-Liou and by NIMHDsupported Research Capacity Core at CAU(U54MD007590)。
基金supported by the National Key Research and Development Program of China(No.2020YFA 0710601)the Deep Earth Probe and Mineral Resources Exploration—National Science and Technology Major Project(No.2025ZD1004901).
文摘With the increasing use of passive seismic data,developing seismic reflection imaging methods based on passive data is of considerable practical significance.This study presents a waveform-matching reverse time migration for the primary reflected data from local earthquakes.In order to mitigate inconsistencies in frequency band and energy across earthquakes of different magnitudes,we first establish reference seismic waveform with standardized dominant frequency and magnitude.A matching operator is derived for each event by matching its waveforms with the reference waveform.This operator is then applied via convolution to all waveforms,producing standardized seismic waveforms with consistent wavelet features.The reshaped waveforms are then subjected to reverse time migration using an impedance imaging condition for primary reflections.To suppress strong energy interference near the hypocenters,both illumination compensation and three-dimensional Smoothed Spherical Mask centered on each source are used.Numerical tests using both simple two-layer model and fault-containing model demonstrate that the new method is robust and effective.The reverse time migration of primary reflected data of local earthquakes accurately images underground impedance boundaries such as stratum interfaces and fault planes,showing its promise for future application in seismically active fault zones.
基金supported by the National Natural Science Foundation of China,No.81571211(to FL)the Natural Science Foundation of Shanghai,No.22ZR1476800(to CH)。
文摘Peripheral nerve defect repair is a complex process that involves multiple cell types;perineurial cells play a pivotal role.Hair follicle neural crest stem cells promote perineurial cell proliferation and migration via paracrine signaling;however,their clinical applications are limited by potential risks such as tumorigenesis and xenogeneic immune rejection,which are similar to the risks associated with other stem cell transplantations.The present study therefore focuses on small extracellular vesicles derived from hair follicle neural crest stem cells,which preserve the bioactive properties of the parent cells while avoiding the transplantation-associated risks.In vitro,small extracellular vesicles derived from hair follicle neural crest stem cells significantly enhanced the proliferation,migration,tube formation,and barrier function of perineurial cells,and subsequently upregulated the expression of tight junction proteins.Furthermore,in a rat model of sciatic nerve defects bridged with silicon tubes,treatment with small extracellular vesicles derived from hair follicle neural crest stem cells resulted in higher tight junction protein expression in perineurial cells,thus facilitating neural tissue regeneration.At 10 weeks post-surgery,rats treated with small extracellular vesicles derived from hair follicle neural crest stem cells exhibited improved nerve function recovery and reduced muscle atrophy.Transcriptomic and micro RNA analyses revealed that small extracellular vesicles derived from hair follicle neural crest stem cells deliver mi R-21-5p,which inhibits mothers against decapentaplegic homolog 7 expression,thereby activating the transforming growth factor-β/mothers against decapentaplegic homolog signaling pathway and upregulating hyaluronan synthase 2 expression,and further enhancing tight junction protein expression.Together,our findings indicate that small extracellular vesicles derived from hair follicle neural crest stem cells promote the proliferation,migration,and tight junction protein formation of perineurial cells.These results provide new insights into peripheral nerve regeneration from the perspective of perineurial cells,and present a novel approach for the clinical treatment of peripheral nerve defects.
文摘The authors regret to report the following error made in“Spatiotemporal dynamics of neuron differentiation and migration in the developing human spinal cord;52(2025)-101283-1295;Doi:https://doi.org/10.1016/j.jgg.2025.08.004”.In Tables S1 and S2 in the supplementary materials of this paper,some items were written in Chinese.The corresponding pictures and tables were not uploaded in time.
基金supported by the National Natural Science Foundation of China(Grant No.31672296).
文摘Ecological barriers present significant challenges to bird migration by limiting the availability of stopover sites and shelters. The Qinghai-Tibet Plateau, a major migratory barrier located in higher latitude Central Asia, exerts a substantial influence on avian migration patterns. Species traversing such ecological barriers may adopt multiple optimal routes, which can contribute to the formation of migratory divides. From 2018 to 2021, the migration routes of 13 adult Common Cuckoos (Cuculus canorus) breeding in the north of the Qinghai-Tibet Plateau were tracked using satellite transmitters. We found Common Cuckoos have two primary migration routes: western and eastern, respectively following western and eastern edges of the Qinghai-Tibet plateau. The eastern and western routes are likely the optimal routes for the Central Asian Common Cuckoos population to navigate the Qinghai-Tibet Plateau. Furthermore, two individuals exhibited intermediate migration routes, suggesting attempted traverses of the Qinghai-Tibet Plateau, although neither completed the migration. These intermediate routes may indicate migratory behavior influenced by hybridization between eastern and western populations or migratory flexibility. Common Cuckoos exhibit significantly faster migration speed, flight speed, and shorter stopover durations during spring compared to autumn. The observed seasonal differences in migration behavior support birds following time-minimization strategies during spring migration. These results revealed the diverse migration routes of Common Cuckoos shaped by the Qinghai-Tibet Plateau and seasonal variation in migration patterns.
基金supported by the Open Research Fund of Key Laboratory of Geological Hazards on Three Gorges Reservoir Area of China Three Gorges University,the Ministry of Education(2020KDZ12)the National Natural Science Foundation Joint Funded Project of China(U2034203),the National Natural Science Foundation Joint Funded Project of China(U22A20600)+2 种基金the Hubei Key Laboratory of Disaster Prevention and Mitigation of China Three Gorges University(2022KJZ08)Natural Science Research Program of Yichang City in 2023(A23-2-047)Scientific Research Program of Hubei Provincial Department of Education in 2022(B2022567)。
文摘This paper aims to elucidate the seismic characteristics of the Three Gorges Reservoir area after impoundment and investigate the seismic source migration.Based on the seismic data analysis from the Badong segment in the Three Gorges Reservoir area,we assessed the local temporal and spatial variations in the frequent earthquakes.Correlation analysis was conducted to investigate the relationship between changes in reservoir water levels and the occurrence of reservoir-induced earthquakes.Additionally,we examined the regularity of earthquake occurrences at the exact location during different periods.Based on the fault mechanics principles,a formula was derived to estimate the length of open and wing-shaped rupture at the hypocenter under the influence of pore or excess pore water pressure.The results reveal that reservoir-induced seismicity demonstrates short-term cycles characterized by alternating"active periods"and"quiet periods,"as well as long-term cycles with the combined periods.The probability of earthquakes occurring within one year at the epicentre is relatively high and decreases after four years.The derived formula can be utilized to estimate the seismic migration distance at the epicentre in the short term.These research findings provide valuable insights for analyzing the regularity of reservoir-induced earthquake activities and understanding the mechanism of seismic source migration.
基金Supported by the National Natural Science Foundation of China(U21B2069,52274020,52288101,52274022)National Key Research and Development Program of China(2022YFC2806504)。
文摘The gas-liquid countercurrent flow pattern is complex and the bubble migration velocity is difficult to predict in the process of bullheading well killing.The experiment on bubble migration in gas-liquid countercurrent flow in annulus is carried out under different working conditions to reveal how the wellbore inclination angle,liquid phase property and countercurrent liquid velocity affect the bubble deformation and bubble migration trajectory/velocity,and to establish a bubble migration velocity prediction model.The bubbles in the countercurrent flow mainly migrate in two modes:free rising of isolated bubbles,and interactive rising of multiple bubbles.The bubbles migrate by an S-shaped trajectory in the countercurrent flow.With the increase of countercurrent liquid velocity,the lateral oscillation of bubbles is intensified.The increases of wellbore inclination angle,liquid density and liquid viscosity make the bubble migration trajectory gradually to be linear.The bubble is generally ellipsoidal during its rising.The wellbore inclination angle has little effect on the degree of bubble deformation.The bubbles are ellipsoidal during rising,with little influence of wellbore inclination angle on bubble deformation.With the increase of liquid viscosity and density,the aspect ratio of the bubble decreases.As the wellbore inclination angle increases,the bubble migration velocity gradually decreases.As the liquid viscosity increases,the bubble migration velocity decreases.As the liquid density increases,the bubble migration velocity increases slightly.The established bubble migration velocity prediction model yields errors within±15%,and demonstrates broad applicability across a wide range of operating conditions.
基金sponsored by Nation 973 Program(Grant No.2007CB209603)Nation 863 projection(Grant No. 2006AA06Z108)+2 种基金Nation 863 Program(Grant No.2007AA06Z215)Young Teachers Innovation Fund of Jilin University(No. 421032124424)Graduate Innovation Fund of Jilin University(Grant No.20101059)
文摘We propose a combined migration velocity analysis and imaging method based on Kirchhoff integral migration and reverse time migration,using the residual curvature analysis and layer stripping strategy to build the velocity model.This method improves the image resolution of Kirchhoff integral migration and reduces the computations of the reverse time migration.It combines the advantages of efficiency and accuracy of the two migration methods.Its application in tunnel seismic prediction shows good results.Numerical experiments show that the imaging results of reverse time migration are better than the imaging results of Kirchhoff integral migration in many aspects of tunnel prediction.Field data show that this method has efficient computations and can establish a reasonable velocity model and a high quality imaging section.Combination with geological information can make an accurate prediction of the front of the tunnel geological structure.
文摘Objective:Ovarian cancer(OC)ranks among the leading causes of mortality among the female cancers worldwide.Numerous studies have explored the development and progression of OC at multiple genetic regulatory levels.However,relatively few studies have explored the impact of post-translational modifications(PTM)on OC progression,which is essential for uncovering new therapeutic targets.This study aimed to systematically identify the key PTM types involved in OCprogression,and to explore and evaluate their translational potential as therapeutic targets.Methods:First,we utilized multiple general PTM antibodies to compare gross PTM levels between normal ovarian and OC tissues from clinical females.After identifying lactylation as the PTM with the most significant differences,we selected representative samples for label-free mass spectrometry to identify specific lactylation sites.Next,we transfected A2780(OC)cells with either wild-type(WT)or mutant(K192A[Q])poly(ADP-ribose)polymerase 1(PARP1)conjugated to enhanced green fluorescent protein(EGFP)with a StrepⅡpeptide tag and assessed various cellular indexes related to cell proliferation(clonogenicity assay),migration(scratch wound healing assay),and reactive oxygen species levels.Results:Pan-lactylation was significantly upregulated in clinical OC samples,with PARP1 lactylation at K192 being one of the most common modifications.The growth and migration of A2780 cells were markedly suppressed by overexpressing PARP1-WT but not mutant PARP1.Overexpressing PARP1 significantly downregulated the phosphorylation of extracellular signal-regulated kinases 1/2(ERK1/2).Conclusion:This study uncovered a novel PTM of PARP1 in OC,lactylation,and demonstrated that lactylation at K192 is crucial in regulating OC cell growth and migration via the ERK1/2 pathway.Further investigations are required to elucidate the broader functional implications of PARP1 lactylation and its therapeutic potential.
