study was conducted on the biologically active secondary metabolites produced by the marine fungus Cladosporium sp.MDW-211.Preparative high performance liquid chromatography(HPLC)and other techniques were used to isol...study was conducted on the biologically active secondary metabolites produced by the marine fungus Cladosporium sp.MDW-211.Preparative high performance liquid chromatography(HPLC)and other techniques were used to isolate and purify the compounds,the structures of the compounds 1 were determined by high-resolution electrospray ionization mass spectrometry(HR-ESI-MS),nuclear magnetic resonance(NMR)spectroscopy,X-ray and other methods,and the pro-angiogenic activity of the compounds was evaluated by zebrafish model.One new dihydroisocoumarin 1(4'S-hydroxyasperentin)and three known dihydroisocoumarins 2~4 were obtained and identified.Bioactivity assays revealed that compound 3 exhibited pro-angiogenic activity at concentrations of 20 and 40μmol/L,which is reported here for the first time.展开更多
文摘目的探讨胶质瘤组织中microRNA-211(miR-211)的表达及其在预测胶质瘤患者预后中的价值。方法 qRT-PCR法检测52例神经胶质瘤组织以及12例正常脑组织中miR-211的表达水平,分析其与胶质瘤患者临床病理特征及其预后之间的关系。结果胶质瘤组织中miR-211的表达水平(0.41±0.24)明显低于正常脑组织(1.00±0.13,P<0.001),并且与WHO分级呈负相关趋势(WHOⅢ-Ⅳ,0.21±0.14 vs WHOⅠ-Ⅱ,0.60±0.18,P=0.016)。胶质瘤组织中miR-211的表达水平与WHO分级和KPS评分密切相关,而与患者年龄、性别、肿瘤位置、肿瘤直径等均无关。miR-211高表达组患者的生存率明显高于miR-211低表达组,差异有统计学意义(P=0.037)。胶质瘤的WHO分级增高、KPS评分<80分及miR-211低表达均是影响胶质瘤患者预后的危险因素。结论 miR-211在胶质瘤组织中呈低表达,与胶质瘤患者预后显著相关,可作为预测胶质瘤患者预后的独立因子及治疗新靶点。
基金Project supported by the National Natural Science Foundation of China(No.82204276)the Guangxi Natural Science Foundation(No.2025GXNSF-AA069557)+1 种基金the Guangxi Scholarship Fund of Guangxi Education Departmentthe Innovation Project of Guangxi Minzu University Graduate Education(No.gxmzu-chxs2024225)。
文摘study was conducted on the biologically active secondary metabolites produced by the marine fungus Cladosporium sp.MDW-211.Preparative high performance liquid chromatography(HPLC)and other techniques were used to isolate and purify the compounds,the structures of the compounds 1 were determined by high-resolution electrospray ionization mass spectrometry(HR-ESI-MS),nuclear magnetic resonance(NMR)spectroscopy,X-ray and other methods,and the pro-angiogenic activity of the compounds was evaluated by zebrafish model.One new dihydroisocoumarin 1(4'S-hydroxyasperentin)and three known dihydroisocoumarins 2~4 were obtained and identified.Bioactivity assays revealed that compound 3 exhibited pro-angiogenic activity at concentrations of 20 and 40μmol/L,which is reported here for the first time.
