The intergeneric hybridization between Brassica napus L. cv. oro and Matthiola incana (L.) R. Br. was carried out to study the phytogenetic peculiarities of the hybrid plants. In order to improve the oil quality of ra...The intergeneric hybridization between Brassica napus L. cv. oro and Matthiola incana (L.) R. Br. was carried out to study the phytogenetic peculiarities of the hybrid plants. In order to improve the oil quality of rape seed, ovaries of B. napus pollinated with pollen of M. incana, were cut off and inoculated onto MS media supplemented with various plant hormones at the 7th day after pollination. Two mature embryos were obtained from 750 pollinated ovaries cultured in vitro. The percentage of seed set was 0.26%. The mature embryos were transferred onto the MS media supplemented with 2.0 mg/L 6-BA + 0.1 mg/L NAA, and a compound bud was produced later. The compound bud was then cut into a number of single buds, which were transferred onto fresh media. Twenty-two plantlets in vitro were developed from the above single buds. The hybrid plants (F-1) were basically intermediate between the two parents in many,characters with a few showing hybrid vigor. The fertility of hybrid plants (F-1) was poor. Cytological studies revealed that the hybrid plants (F-1) were mixoploid. in nature. The chromosome number of many somatic cells was 2n = 26, which was the sum of the chromosome number of the two parents. The chromosome number of other somatic cells was 2n = 38, similar to that of B. napus. The hybrid offspring (F-2) from the selfed hybrid plants (F-1) showed polymorphism. Among the hybrid offspring (F-2) some were nearly matroclinous and fertile, similar to B. napus. some others were intermediate between the two parents and less fertile, and a few were poorly developed and nearly infertile. From the hybrid offspring a few plants with improved seed-oil quality were obtained.展开更多
[ Objective] The paper aimed to study the optimum extraction technology of pigments from petals of Matthiola incana（ L. ） R. Br. of differ- ent colors. [Method] Petal pigments of Aida variety （white violet）, Franc...[ Objective] The paper aimed to study the optimum extraction technology of pigments from petals of Matthiola incana（ L. ） R. Br. of differ- ent colors. [Method] Petal pigments of Aida variety （white violet）, Francesco variety （ red violet） and Arabella variety （ purple violet） were ana- lyzed by colorimetric reaction method and ultraviolet-visible spectrum method, and the optimum extraction technology of flavonoid compounds from petals of them was determined by single factor experiment and orthogonal experiment. [ Result] The optimum extraction conditions of white violet were as follows： with 95% ethanol ＋ 5% HCI as solvent, solid-liquid ratio （ 1 ： 40）, extraction time （3 h）, extraction temperature （65 ℃ ）. The opti- mum extraction conditions of red violet and purple violet were as follows： with acetone as solvent, solid-liquid ratio （1：40）, extraction time （3 h）, extraction temperature （65 ℃）. [ Conclusion] The study provided theoretical foundation for further development and exploitation of pigments from M. incana（ L. ） R. Br.展开更多
文摘The intergeneric hybridization between Brassica napus L. cv. oro and Matthiola incana (L.) R. Br. was carried out to study the phytogenetic peculiarities of the hybrid plants. In order to improve the oil quality of rape seed, ovaries of B. napus pollinated with pollen of M. incana, were cut off and inoculated onto MS media supplemented with various plant hormones at the 7th day after pollination. Two mature embryos were obtained from 750 pollinated ovaries cultured in vitro. The percentage of seed set was 0.26%. The mature embryos were transferred onto the MS media supplemented with 2.0 mg/L 6-BA + 0.1 mg/L NAA, and a compound bud was produced later. The compound bud was then cut into a number of single buds, which were transferred onto fresh media. Twenty-two plantlets in vitro were developed from the above single buds. The hybrid plants (F-1) were basically intermediate between the two parents in many,characters with a few showing hybrid vigor. The fertility of hybrid plants (F-1) was poor. Cytological studies revealed that the hybrid plants (F-1) were mixoploid. in nature. The chromosome number of many somatic cells was 2n = 26, which was the sum of the chromosome number of the two parents. The chromosome number of other somatic cells was 2n = 38, similar to that of B. napus. The hybrid offspring (F-2) from the selfed hybrid plants (F-1) showed polymorphism. Among the hybrid offspring (F-2) some were nearly matroclinous and fertile, similar to B. napus. some others were intermediate between the two parents and less fertile, and a few were poorly developed and nearly infertile. From the hybrid offspring a few plants with improved seed-oil quality were obtained.
基金Supported by Natural Science Foundation in Chongqing(CSTC,2006BB1336)Applied Basic Research Program of Chongqing Education Department(KJ060308)
文摘[ Objective] The paper aimed to study the optimum extraction technology of pigments from petals of Matthiola incana（ L. ） R. Br. of differ- ent colors. [Method] Petal pigments of Aida variety （white violet）, Francesco variety （ red violet） and Arabella variety （ purple violet） were ana- lyzed by colorimetric reaction method and ultraviolet-visible spectrum method, and the optimum extraction technology of flavonoid compounds from petals of them was determined by single factor experiment and orthogonal experiment. [ Result] The optimum extraction conditions of white violet were as follows： with 95% ethanol ＋ 5% HCI as solvent, solid-liquid ratio （ 1 ： 40）, extraction time （3 h）, extraction temperature （65 ℃ ）. The opti- mum extraction conditions of red violet and purple violet were as follows： with acetone as solvent, solid-liquid ratio （1：40）, extraction time （3 h）, extraction temperature （65 ℃）. [ Conclusion] The study provided theoretical foundation for further development and exploitation of pigments from M. incana（ L. ） R. Br.
