Objective To evaluate the clinical efficacy of incising spinal pia mater to relieve pressure and unilateral open-door laminoplasty with internal screw fixation for treatment of the dated spinal cord injury. Methods Fr...Objective To evaluate the clinical efficacy of incising spinal pia mater to relieve pressure and unilateral open-door laminoplasty with internal screw fixation for treatment of the dated spinal cord injury. Methods From March, 2009 to July, 2010, 16 cases with chronic cervical cord injury underwent spinal dura mater incision and unilateral open-door laminoplasty with internal screw fixation. Nerve functions of preand postoperation were evaluated by Frankel classification and the Japanese Orthopaedic Association (JOA) scale. The improvement rate of JOA score at the indicated time was recorded. Results Postoperative Frankel classification rating of 16 patients improved obviously. JOA scores at the 1st month, 3rd month, 6th month, and 12th month after surgery were 7.9±2.3, 8.5±1.6, 8.9±2.1, and 12.4±2.5, respectively, and significantly increased compared with that prior to surgery (5.5±0.6). At the end of follow-up period, JOA score was significantly higher than that of pre-treatment (P<0.05). The recovery was relatively rapid during the first 3 months following the surgery, then entered a platform period. Conclusion It is effective for patients with dated spinal cord injury to undergo spinal decompression and laminoplasty.展开更多
With chromium-hematoxylin staining, we found evidence for the existence of novel age-dependent network structures in the dura mater of rat brains. Under stereomicroscopy, we noticed that chromium-hematoxylin-stained t...With chromium-hematoxylin staining, we found evidence for the existence of novel age-dependent network structures in the dura mater of rat brains. Under stereomicroscopy, we noticed that chromium-hematoxylin-stained threadlike structures, which were barely observable in 1-weekold rats, were networked in specific areas of the brain, for example, the lateral lobes and the cerebella, in 4-week-old rats. In 7-week-old rats, those structures were found to have become larger and better networked. With phase contrast microscopy, we found that in 1-week-old rats, chromium-hematoxylin-stained granules were scattered in the same areas of the brain in which the network structures would later be observed in the 4- and 7-week-old rats. Such age-dependent network structures were examined by using optical and transmission electron microscopy, and the following results were obtained. The scattered granules fused into networks with increasing age. Cross-sections of the age-dependent network structures demonstrated heavily-stained basophilic substructures. Transmission electron microscopy revealed the basophilic substructures to be clusters with high electron densities consisting of nanosized particles. We report these data as evidence for the existence of age-dependent network structures in the dura mater, we discuss their putative functions of age-dependent network structures beyond the general concept of the dura mater as a supporting matrix.展开更多
Effects of electroacupuncture (EA) and routine acupuncture with twirling reinforcing and reducing manipulation of the needle (RA) both at Zusanli point (St 36) on volume of microcirculatory blood flow in the cerebral ...Effects of electroacupuncture (EA) and routine acupuncture with twirling reinforcing and reducing manipulation of the needle (RA) both at Zusanli point (St 36) on volume of microcirculatory blood flow in the cerebral pia mater were observed by fenestration of the cranial bone and laser Doppler microcirculatory blood flow analyser. Results showed that both RA and EA could increase the volume of microcirculatory blood flow in the cerebral pia mater; and that the increase in the EA group was superior to that in RA group. This suggests that a moderate and effective stimulation is a key to the production of a regulative effect on the organism.展开更多
Two new chiral Schiff base acacen-derivative nickel（II） and copper（II） complexes have been prepared and characterized with IR, electronic, and CD spectra and X-ray crystallography. So-called artifact peaks of soli...Two new chiral Schiff base acacen-derivative nickel（II） and copper（II） complexes have been prepared and characterized with IR, electronic, and CD spectra and X-ray crystallography. So-called artifact peaks of solid-state CD spectra due to restricted to rotate freely of molecules appeared at about 334 and 460 nm for nickel（II） and copper（II） complexes. Changes of intensity of the peaks have been investigated in different matrices such as solid-state （microcrystals or KBr pellets）, PMMA cast films and acetone solutions of various concentrations and pure acetone solutions. Although restricted orientation of molecules in rigid matrices leads to increase the peak intensity toward negative and positive optical rotation for nickel（II） and copper（II） complexes, respectively, the degree of increasing intensity depends on not concentration but viscosity of polymer solutions. Therefore, the artifact CD peaks of solid-state can act as an indicator of environmental viscosity of soft mater matrices.展开更多
The dura mater is of similar embryological origin to the fascial organ. It contains several fibroblasts which make the dura mater a flexible structure. Dura mater is the outermost of the three layers of meninges, a th...The dura mater is of similar embryological origin to the fascial organ. It contains several fibroblasts which make the dura mater a flexible structure. Dura mater is the outermost of the three layers of meninges, a thick and rigid inelastic membrane that covers the brain and spinal cord and that is impermeable to the cerebrospinal fluid. The cranial dura mater in certain respects differs anatomically from the dura material in the spinal cord, and it is important to classify them separately. This article reviews the anatomical structure of spinal and cranial dura mater and its anatomy with muscle, fascia, bone structure and ligaments. Dura mater is a structure in the body that is connected with systemic functions.展开更多
Rare earth-containing magneto-strictive material has caught theworld attention in recent yearsbecause of its amazing performan-ces:high magnetostrictive ratio,rapid response to mechanical be-havior,high rate of energy...Rare earth-containing magneto-strictive material has caught theworld attention in recent yearsbecause of its amazing performan-ces:high magnetostrictive ratio,rapid response to mechanical be-havior,high rate of energy tran-sformation,etc.,which are vitalto sonar transducers,performersand brakes which,in turn,play展开更多
We reported the earliest cases involving electrostatic spinning synthetic dura substitutes for repairing defects in the spinal dura mater. From June to September 2017, seven patients were treated with electrostatic sp...We reported the earliest cases involving electrostatic spinning synthetic dura substitutes for repairing defects in the spinal dura mater. From June to September 2017, seven patients were treated with electrostatic spinning synthetic dura substitute. The laminotomy was performed for all the patients, and the cancers were resected, then the electrostatic spinning synthetic dura substitute made of polylactic acid ReDuraTM. All of the patients had no complications and might be the ideal promising alternative for repairing the spinal dura mater.展开更多
Aims: To examine the associations between being overweight and behavioural pro blems at ages 5 and 14. Methods: Birth cohort study of 2875 individuals who were born in Brisbane between 1981 and 1984 and who were follo...Aims: To examine the associations between being overweight and behavioural pro blems at ages 5 and 14. Methods: Birth cohort study of 2875 individuals who were born in Brisbane between 1981 and 1984 and who were followed up at ages 5 and 1 4 years. Behavioural problems were defined as scoring above the 90th centile on Achenbach’s child behavioural checklist. Results: In cross-sectional analyses there was no association between being overweight and behavioural problems in ei ther females at age 5. At age 14 females who were overweight were more likely th an those who were normal weight to experience behavioural problems. However, the re was no association between being overweight and behavioural problems at age 1 4 among males. The prevalence of behavioural problems increased linearly across the di stribution of body mass index in females at age 14. In prospective analyses, amo ng participants who had no behavioural problems at age 5, there was no associati on between being overweight at age 5 and behavioural problems at age 14 in eithe r sex. Females who were overweight at age 5 and normal weight at age 14 had redu ced odds of behavioural problems at age 14. Conclusions: Among adolescent female s there is a positive linear association between body size and behavioural probl ems. However, no such association was found in adolescent males, or in either se x at age 5 years, and in prospective analyses being overweight at age 5 was not associated with behavioural problems in either sex at age 14.展开更多
背景:许多研究将静电纺丝应用于人工硬膜替代物的研究中,设计了具有多功能的人工硬膜,不仅可以更好地模拟天然硬膜结构,还能发挥促愈合、防粘连、抗菌等生物学功能。目的:系统总结静电纺丝技术应用于人工硬膜替代物中的研究进展。方法:...背景:许多研究将静电纺丝应用于人工硬膜替代物的研究中,设计了具有多功能的人工硬膜,不仅可以更好地模拟天然硬膜结构,还能发挥促愈合、防粘连、抗菌等生物学功能。目的:系统总结静电纺丝技术应用于人工硬膜替代物中的研究进展。方法:以“静电纺丝硬膜,硬膜替代物,人工硬膜补片”为中文检索词,以“electrospinning dura mater,dura mater substitute,artificial dura mater patch”为英文检索词,分别检索中国知网、万方数据库和PubMed数据库,文献检索时限为各数据库建库至2024年9月,通过阅读题目和摘要进行初步筛选,排除与文章相关性较低的文献,最终纳入64篇文献进行归纳总结。结果与结论:人工硬膜主要分为不可降解和可降解两大类,在修复硬膜损伤中发挥重要作用,也是目前生物组织工程研究的热点。静电纺丝技术可通过结合化学、材料学、药学等学科从结构和功能需求上进行人工硬膜替代物的设计,制备出仿生取向纤维型、不对称双层型、多层复合型等一系列新型人工硬膜,同时还赋予人工硬膜密封、防粘连、抗菌和促进神经组织修复等多种重要的生物功能,使得人工硬膜由过去传统的单纯补片转变为多功能的组织生物支架。静电纺丝为以后开发和研究更加理想的人工硬膜替代物提供了帮助,也为解决硬膜损伤修复问题带来新的思路。展开更多
Background Intracranial infection is one of the most common complications of open craniocerebral injury and of conventional craniotomy in neurosurgery. The presence of blood-brain barrier leads to lower drug concentra...Background Intracranial infection is one of the most common complications of open craniocerebral injury and of conventional craniotomy in neurosurgery. The presence of blood-brain barrier leads to lower drug concentrations in the cerebrospinal fluid than in the venous blood. Increasing the intravenous dosage or frequency carries the risk of systemic adverse reactions or infections in other parts of the body. Developing an artificial dura mater （ADM） for sustained antibiotic release for use during neurosurgery can solve the problems perfectly. Methods Three types of drug-loaded ADMs made of collagen and containing cefuroxime sodium, ceftriaxone sodium, or norvancomycin were prepared. The antibacterial activity and sustained release characteristics of the ADMs were examined using bacteriostatic and release tests. Results Single-layered collagen based ADMs （40 mm×50 mm×5 mm） containing 18 mg cefuroxime sodium or ceftriaxone sodium were not suitable for continued development because of drug preservation and stability issues. Using smaller ADMs （20 mm30 mm×7 mm）, containing 4.86 mg of norvancomycin, with increased collagen density and a three-layered film with two outer drug-free films above and below the antibiotic layer resulted in sustained cumulative release of 2.91 mg （59.9%） of norvancomycin over 72 hours. The similar factor （f2） comparison method proved that products from a same batch were statistically significant similar （f2 〉50）. Conclusions Artificial ADMs made of collagen can be processed to provide a mature dural repair material for the sustained release of norvancomycin. This system may provide a basis for developing sustained release materials for other drugs.展开更多
In the field of dura mater repair,it is essential to employ artificial substitutes mimicking the multilayered microar-chitecture and multiple functions of native dura mater for effective neurosurgery.However,existing ...In the field of dura mater repair,it is essential to employ artificial substitutes mimicking the multilayered microar-chitecture and multiple functions of native dura mater for effective neurosurgery.However,existing artificial dura mater substitutes commonly cause complications because of mismatched structural and mechanical properties as well as the lack of antibacterial activity or osteogenic capability.In this study,a triple-layered dura mater substi-tute was fabricated by electrohydrodynamic(EHD)jetting techniques,including electrospinning and melt-based EHD printing processes.Highly aligned polycaprolactone(PCL)nanofibers loaded with gentamicin sulfate(GS)were prepared by electrospinning to form the inner layer,which can mimic the aligned collagen fibers of the native dura mater.Random PCL-GS nanofibers were then deposited by electrospinning to form the middle layer.They were intended to enhance the mechanical properties of the fabricated scaffolds.The outer layer involv-ing PCL microfibers doped with nano-hydroxyapatite(nHA)at various angles was printed by the melting-based EHD method,which can enhance osteogenic capability and promote the fusion between the dura mater substi-tute and the skull.The tensile strength of the triple-layered drug-loaded biomimetic dura mater substitute was 22.42±0.89 MPa,and the elongation at break was 36.43%±2.00%.The addition of GS endowed the substitutes with an anti-infection property without influencing their cytocompatibility.Furthermore,the incorporation of nHA promoted the osteogenic differentiation of MC3T3-E1 cells seeded on the triple-layered scaffolds.This work offers a promising strategy to manufacture multilayered dura mater substitutes with the desired antibacterial and enhanced osteogenic capability performance,possibly providing a novel candidate for dural tissue repair.展开更多
文摘Objective To evaluate the clinical efficacy of incising spinal pia mater to relieve pressure and unilateral open-door laminoplasty with internal screw fixation for treatment of the dated spinal cord injury. Methods From March, 2009 to July, 2010, 16 cases with chronic cervical cord injury underwent spinal dura mater incision and unilateral open-door laminoplasty with internal screw fixation. Nerve functions of preand postoperation were evaluated by Frankel classification and the Japanese Orthopaedic Association (JOA) scale. The improvement rate of JOA score at the indicated time was recorded. Results Postoperative Frankel classification rating of 16 patients improved obviously. JOA scores at the 1st month, 3rd month, 6th month, and 12th month after surgery were 7.9±2.3, 8.5±1.6, 8.9±2.1, and 12.4±2.5, respectively, and significantly increased compared with that prior to surgery (5.5±0.6). At the end of follow-up period, JOA score was significantly higher than that of pre-treatment (P<0.05). The recovery was relatively rapid during the first 3 months following the surgery, then entered a platform period. Conclusion It is effective for patients with dated spinal cord injury to undergo spinal decompression and laminoplasty.
基金supported by Global Ph.D.Fellowship Program through the National Research Foundation of Korea(NRF)funded by the Ministry of Education(2014H1A2A1020588)by Korea Atomic Energy Research Institute,by a Korean Pharmacopuncture Foundation Grant funded by the Korean Pharmacopuncture Institute(KPI-2014-010)by the grant K13290 of KIOM and by Basic Science Research Program through the National Research Foundation of Korea(NRF)funded by the Ministry of Science,ICT and Future Planning(2013R1A1A2021577)
文摘With chromium-hematoxylin staining, we found evidence for the existence of novel age-dependent network structures in the dura mater of rat brains. Under stereomicroscopy, we noticed that chromium-hematoxylin-stained threadlike structures, which were barely observable in 1-weekold rats, were networked in specific areas of the brain, for example, the lateral lobes and the cerebella, in 4-week-old rats. In 7-week-old rats, those structures were found to have become larger and better networked. With phase contrast microscopy, we found that in 1-week-old rats, chromium-hematoxylin-stained granules were scattered in the same areas of the brain in which the network structures would later be observed in the 4- and 7-week-old rats. Such age-dependent network structures were examined by using optical and transmission electron microscopy, and the following results were obtained. The scattered granules fused into networks with increasing age. Cross-sections of the age-dependent network structures demonstrated heavily-stained basophilic substructures. Transmission electron microscopy revealed the basophilic substructures to be clusters with high electron densities consisting of nanosized particles. We report these data as evidence for the existence of age-dependent network structures in the dura mater, we discuss their putative functions of age-dependent network structures beyond the general concept of the dura mater as a supporting matrix.
基金The study has been supported by Fund of National Administrative Bureau of TCM for the Young, Grant No. 93C025
文摘Effects of electroacupuncture (EA) and routine acupuncture with twirling reinforcing and reducing manipulation of the needle (RA) both at Zusanli point (St 36) on volume of microcirculatory blood flow in the cerebral pia mater were observed by fenestration of the cranial bone and laser Doppler microcirculatory blood flow analyser. Results showed that both RA and EA could increase the volume of microcirculatory blood flow in the cerebral pia mater; and that the increase in the EA group was superior to that in RA group. This suggests that a moderate and effective stimulation is a key to the production of a regulative effect on the organism.
文摘Two new chiral Schiff base acacen-derivative nickel（II） and copper（II） complexes have been prepared and characterized with IR, electronic, and CD spectra and X-ray crystallography. So-called artifact peaks of solid-state CD spectra due to restricted to rotate freely of molecules appeared at about 334 and 460 nm for nickel（II） and copper（II） complexes. Changes of intensity of the peaks have been investigated in different matrices such as solid-state （microcrystals or KBr pellets）, PMMA cast films and acetone solutions of various concentrations and pure acetone solutions. Although restricted orientation of molecules in rigid matrices leads to increase the peak intensity toward negative and positive optical rotation for nickel（II） and copper（II） complexes, respectively, the degree of increasing intensity depends on not concentration but viscosity of polymer solutions. Therefore, the artifact CD peaks of solid-state can act as an indicator of environmental viscosity of soft mater matrices.
文摘The dura mater is of similar embryological origin to the fascial organ. It contains several fibroblasts which make the dura mater a flexible structure. Dura mater is the outermost of the three layers of meninges, a thick and rigid inelastic membrane that covers the brain and spinal cord and that is impermeable to the cerebrospinal fluid. The cranial dura mater in certain respects differs anatomically from the dura material in the spinal cord, and it is important to classify them separately. This article reviews the anatomical structure of spinal and cranial dura mater and its anatomy with muscle, fascia, bone structure and ligaments. Dura mater is a structure in the body that is connected with systemic functions.
文摘Rare earth-containing magneto-strictive material has caught theworld attention in recent yearsbecause of its amazing performan-ces:high magnetostrictive ratio,rapid response to mechanical be-havior,high rate of energy tran-sformation,etc.,which are vitalto sonar transducers,performersand brakes which,in turn,play
文摘We reported the earliest cases involving electrostatic spinning synthetic dura substitutes for repairing defects in the spinal dura mater. From June to September 2017, seven patients were treated with electrostatic spinning synthetic dura substitute. The laminotomy was performed for all the patients, and the cancers were resected, then the electrostatic spinning synthetic dura substitute made of polylactic acid ReDuraTM. All of the patients had no complications and might be the ideal promising alternative for repairing the spinal dura mater.
文摘Aims: To examine the associations between being overweight and behavioural pro blems at ages 5 and 14. Methods: Birth cohort study of 2875 individuals who were born in Brisbane between 1981 and 1984 and who were followed up at ages 5 and 1 4 years. Behavioural problems were defined as scoring above the 90th centile on Achenbach’s child behavioural checklist. Results: In cross-sectional analyses there was no association between being overweight and behavioural problems in ei ther females at age 5. At age 14 females who were overweight were more likely th an those who were normal weight to experience behavioural problems. However, the re was no association between being overweight and behavioural problems at age 1 4 among males. The prevalence of behavioural problems increased linearly across the di stribution of body mass index in females at age 14. In prospective analyses, amo ng participants who had no behavioural problems at age 5, there was no associati on between being overweight at age 5 and behavioural problems at age 14 in eithe r sex. Females who were overweight at age 5 and normal weight at age 14 had redu ced odds of behavioural problems at age 14. Conclusions: Among adolescent female s there is a positive linear association between body size and behavioural probl ems. However, no such association was found in adolescent males, or in either se x at age 5 years, and in prospective analyses being overweight at age 5 was not associated with behavioural problems in either sex at age 14.
文摘背景:许多研究将静电纺丝应用于人工硬膜替代物的研究中,设计了具有多功能的人工硬膜,不仅可以更好地模拟天然硬膜结构,还能发挥促愈合、防粘连、抗菌等生物学功能。目的:系统总结静电纺丝技术应用于人工硬膜替代物中的研究进展。方法:以“静电纺丝硬膜,硬膜替代物,人工硬膜补片”为中文检索词,以“electrospinning dura mater,dura mater substitute,artificial dura mater patch”为英文检索词,分别检索中国知网、万方数据库和PubMed数据库,文献检索时限为各数据库建库至2024年9月,通过阅读题目和摘要进行初步筛选,排除与文章相关性较低的文献,最终纳入64篇文献进行归纳总结。结果与结论:人工硬膜主要分为不可降解和可降解两大类,在修复硬膜损伤中发挥重要作用,也是目前生物组织工程研究的热点。静电纺丝技术可通过结合化学、材料学、药学等学科从结构和功能需求上进行人工硬膜替代物的设计,制备出仿生取向纤维型、不对称双层型、多层复合型等一系列新型人工硬膜,同时还赋予人工硬膜密封、防粘连、抗菌和促进神经组织修复等多种重要的生物功能,使得人工硬膜由过去传统的单纯补片转变为多功能的组织生物支架。静电纺丝为以后开发和研究更加理想的人工硬膜替代物提供了帮助,也为解决硬膜损伤修复问题带来新的思路。
基金This work was supported by a grant of the National Natural Science Foundation of China （No. 81070978）. Conflicts of interest： None.
文摘Background Intracranial infection is one of the most common complications of open craniocerebral injury and of conventional craniotomy in neurosurgery. The presence of blood-brain barrier leads to lower drug concentrations in the cerebrospinal fluid than in the venous blood. Increasing the intravenous dosage or frequency carries the risk of systemic adverse reactions or infections in other parts of the body. Developing an artificial dura mater （ADM） for sustained antibiotic release for use during neurosurgery can solve the problems perfectly. Methods Three types of drug-loaded ADMs made of collagen and containing cefuroxime sodium, ceftriaxone sodium, or norvancomycin were prepared. The antibacterial activity and sustained release characteristics of the ADMs were examined using bacteriostatic and release tests. Results Single-layered collagen based ADMs （40 mm×50 mm×5 mm） containing 18 mg cefuroxime sodium or ceftriaxone sodium were not suitable for continued development because of drug preservation and stability issues. Using smaller ADMs （20 mm30 mm×7 mm）, containing 4.86 mg of norvancomycin, with increased collagen density and a three-layered film with two outer drug-free films above and below the antibiotic layer resulted in sustained cumulative release of 2.91 mg （59.9%） of norvancomycin over 72 hours. The similar factor （f2） comparison method proved that products from a same batch were statistically significant similar （f2 〉50）. Conclusions Artificial ADMs made of collagen can be processed to provide a mature dural repair material for the sustained release of norvancomycin. This system may provide a basis for developing sustained release materials for other drugs.
基金supported by National Key Research and Develop-ment Program of China(Grant No.2018YFA0703000)OPEN Project(Grant No.BHJ17C019),National Natural Science Foundation of China(Grant No.52125501)+4 种基金Key Research Project of Shaanxi Province(Grant Nos.2020GXLH-Y-021,2021GXLH-Z-028)Guangdong Basic and Ap-plied Basic Research Foundation(Grant No.2020B1515130002)Inno-vation Cultivation Fund of the Sixth Medical Center of PLA General Hos-pital(Grant No.CXPY201913)China Postdoctoral Science Foundation Funded Project(Grant No.2021M702597)Youth Innovation Team of Shaanxi Universities and the Fundamental Research Funds for the Central Universities.
文摘In the field of dura mater repair,it is essential to employ artificial substitutes mimicking the multilayered microar-chitecture and multiple functions of native dura mater for effective neurosurgery.However,existing artificial dura mater substitutes commonly cause complications because of mismatched structural and mechanical properties as well as the lack of antibacterial activity or osteogenic capability.In this study,a triple-layered dura mater substi-tute was fabricated by electrohydrodynamic(EHD)jetting techniques,including electrospinning and melt-based EHD printing processes.Highly aligned polycaprolactone(PCL)nanofibers loaded with gentamicin sulfate(GS)were prepared by electrospinning to form the inner layer,which can mimic the aligned collagen fibers of the native dura mater.Random PCL-GS nanofibers were then deposited by electrospinning to form the middle layer.They were intended to enhance the mechanical properties of the fabricated scaffolds.The outer layer involv-ing PCL microfibers doped with nano-hydroxyapatite(nHA)at various angles was printed by the melting-based EHD method,which can enhance osteogenic capability and promote the fusion between the dura mater substi-tute and the skull.The tensile strength of the triple-layered drug-loaded biomimetic dura mater substitute was 22.42±0.89 MPa,and the elongation at break was 36.43%±2.00%.The addition of GS endowed the substitutes with an anti-infection property without influencing their cytocompatibility.Furthermore,the incorporation of nHA promoted the osteogenic differentiation of MC3T3-E1 cells seeded on the triple-layered scaffolds.This work offers a promising strategy to manufacture multilayered dura mater substitutes with the desired antibacterial and enhanced osteogenic capability performance,possibly providing a novel candidate for dural tissue repair.
