Serum immunoglobulin from the mandarin fish, or the so called Chinese perch, Siniperca chuatsi (Basilewsky), was successfully purified using affinity chromatography. Heavy and light chains were detected on electrophor...Serum immunoglobulin from the mandarin fish, or the so called Chinese perch, Siniperca chuatsi (Basilewsky), was successfully purified using affinity chromatography. Heavy and light chains were detected on electrophoresis gel, with molecular weights being estimated at 72 and 29 kDa, respectively. The tetrameric IgM of S. chuatsi was calculated to be 808 kDa. The rabbit polyclonal antisera against the purifed immunoglobulin were developed and tested by Western blot analysis. The antisera reacted strongly with the heavy chains of S. chuatsi immunoglobulin. Humoral immune responses of the mandarin fish can then be examined using the developed polyclonal antibody.展开更多
Carnivorous fish have poor tolerance to carbohydrate in feed and low utilization rate of carbohydrate.Therefore,the balance of carbohydrate and lipids in the nutrient metabolism of carnivorous fish,the ef fective conv...Carnivorous fish have poor tolerance to carbohydrate in feed and low utilization rate of carbohydrate.Therefore,the balance of carbohydrate and lipids in the nutrient metabolism of carnivorous fish,the ef fective conversion and utilization of carbohydrate and lipids,and the feedback regulation of feeding are the key links for the e fficient utilization of carnivorous fish feed.Carbohydrate response element binding protein(ChREBP)is a new transcription factor found in recent years in the glucose signaling pathway,and can also participate in feeding regulation.We performed in-vivo and in-vitro experiments to reveal the role of ChREBP in the glucose metabolism and feeding in mandarin fish.The mRNA expression of ChREBP and appetite regulatory factors were measured after intraperitoneal injection of glucose in mandarin fish Siniperca chuatsi and cotransfection with glucose and glucose+siRNA in the hypothalamic cells in mandarin fish.The results reveal that at hour 2 and 4 post intraperitoneal injection with 1 mg/g BW glucose,the blood glucose level of the mandarin fish increased significantly,but food intake decreased significantly,and it also displayed a significantly increased ChREBP mRNA expression levels in liver.At hour 4 post injection,hypothalamic ChREBP mRNA level was significantly increased,whereas the mRNA expression levels of appetite genes neuropeptide Y(npy)and agouti-related peptide(AgRP)were decreased significantly.When the glucose concentration was 40 mmol/L,the expression level of ChREBP mRNA in mandarin fish hypothalamic cells was significantly up-regulated,but the expression level of appetite gene npy mRNA was down-regulated.When siRNA and glucose were co-transfected into mandarin fish brain cells,the expression level of chrebp mRNA was significantly decreased,and the appetite gene npy mRNA was significantly increased.The results indicated that glucose regulated food intake through the modulation of appetite gene npy by ChREBP.展开更多
The genomic and cDNA sequences of the CD3γ/δ and CD3ε homologues in the mandarin fish, Siniperca chuatsi, were determined. As in other vertebrate CD3 molecules, the deduced amino acid sequences of mandarin fish CD3...The genomic and cDNA sequences of the CD3γ/δ and CD3ε homologues in the mandarin fish, Siniperca chuatsi, were determined. As in other vertebrate CD3 molecules, the deduced amino acid sequences of mandarin fish CD3γ/δ and CD3ε contained conserved residues and motifs, such as cysteine residues and CXXC and immunoreceptor tyrosine-based activation motifs. However, mandarin fish CD3γ/δ and CD3ε showed some differences to their mammalian counterparts, specifically the absence of a negatively charged residue in the transmembrane region of CD3γ/δ. Additionally, while an N-glycosylation site was present in CD3c, the site was not observed in CD3γ/δ. The CD3γ/δ and CD3ε subunit sequences contain six and five exons, respectively, consistent with homologues from Atlantic salmon, Salmo salar. Phylogenetic analysis also revealed that CD3γ/δ and CD3ε in mandarin fish are closely related to their counterparts in Acanthopterygian fish. Real-time PCR showed CD3γ/δ and CD3ε were expressed mainly in the thymus and spleen in normal healthy fish and, to a lesser extent, in mucosal-associated lymphoid tissues, such as the intestine and gills. When lymphocytes isolated from head kidney were treated with the mitogens phytohemagglutinin, concanavalin, and polyriboinosinic polyribocytidylic acid, mRNA expression levels of CD3γ/δ and CD3ε were significantly elevated within 12 h of treatment. This indicated the presence of T lymphocytes in the head kidney of teleost fish, and also the recognition of mitogens by the lymphocytes. Mandarin fish infected with the bacterial pathogen Flavobacterium columnare also showed an increase in the expression of CD3γ/δ and CD3ε mR_NA, indicating that CD3γ/δ and CD3ε lymphocytes are involved in the immune response of this species.展开更多
The von Hippel-Lindau tumor suppressor protein(VHL),an E3 ubiquitin ligase,functions as a critical regulator of the oxygen-sensing pathway for targeting hypoxia-inducible factors.Recent evidence suggests that mammalia...The von Hippel-Lindau tumor suppressor protein(VHL),an E3 ubiquitin ligase,functions as a critical regulator of the oxygen-sensing pathway for targeting hypoxia-inducible factors.Recent evidence suggests that mammalian VHL may also be critical to the NF-κB signaling pathway,although the specific molecular mechanisms remain unclear.Herein,the roles of mandarin fish(Siniperca chuatsi)VHL(scVHL)in the NF-κB signaling pathway and mandarin fish ranavirus(MRV)replication were explored.The transcription of scVHL was induced by immune stimulation and MRV infection,indicating a potential role in innate immunity.Dual-luciferase reporter gene assays and reverse transcription quantitative PCR(RT-qPCR)results demonstrated that scVHL evoked and positively regulated the NF-κB signaling pathway.Treatment with NF-κB signaling pathway inhibitors indicated that the role of scVHL may be mediated through scIKKα,scIKKβ,scIκBα,or scp65.Co-immunoprecipitation(Co-IP)analysis identified scIκBαas a novel target protein of scVHL.Moreover,scVHL targeted scIκBαto catalyze the formation of K63-linked polyubiquitin chains to activate the NF-κB signaling pathway.Following MRV infection,NF-κB signaling remained activated,which,in turn,promoted MRV replication.These findings suggest that scVHL not only positively regulates NF-κB but also significantly enhances MRV replication.This study reveals a novel function of scVHL in NF-κB signaling and viral infection in fish.展开更多
The heat shock protein 70(HSP70)in fish plays a crucial role in various physiological and environmental stresses.To reveal the mechanisms of the HSP70 gene response to abiotic stress in mandarin fish(Siniperca chuatsi...The heat shock protein 70(HSP70)in fish plays a crucial role in various physiological and environmental stresses.To reveal the mechanisms of the HSP70 gene response to abiotic stress in mandarin fish(Siniperca chuatsi),we analyzed the HSP70 gene family in this species.There are 16 HSP70 genes in mandarin fish,and their protein three-dimensional structures were constructed.The aliphatic index of the 16 mandarin fish HSP70sranges from 73.50(HSPa4b)to 99.46(HSPa13),and all are hydrophilic proteins,with seven of them being unstable proteins.After exposure to chrysene,six HSP70 genes in the intestine,four in the liver,and four in the brain tissues exhibited significant expression changes.These findings reveal that chrysene exposure affects the physiological status of fish,and HSP70 genes may play a role in the chrysene exposure tolerance of the intestine,brain and liver in mandarin fish.展开更多
To enrich the genomic information of the commercially important fish species, we obtained 5,063 high-quality expressed sequence tags （ESTs） from the muscle cDNA database of the mandarin fish （Siniperca chuatsi）. C...To enrich the genomic information of the commercially important fish species, we obtained 5,063 high-quality expressed sequence tags （ESTs） from the muscle cDNA database of the mandarin fish （Siniperca chuatsi）. Clustering analysis yielded 1,625 unique sequences including 443 contigs （from 3,881 EST sequences） and 1,182 sin- gletons. BLASTX searches showed that 959 unique sequences shared homology to proteins in the NCBI non-redundant database. A total of 740 unique sequences were functionally annotated using Gene Ontology. The 1,625 unique sequences were assigned to Kyoto Encyclopedia of Genes and Genomes reference pathways, and the results indicated that transcripts participating in nucleotide metabolism and amino acid metabolism are relatively abundant in S. chuatsi. Meanwhile, we identified 15 genes to be abundantly expressed in muscle of the mandarin fish. These genes are involved in muscle structural formation and regulation of muscle differentiation and development. The most remarkable gene in S. chuatsi is nuclease diphosphate kinase B, which is represented by 449 EST sequences accounting for 8.86% of the total EST sequences. Our work provides a transcript profile expressed in the white muscle of the mandarin fish, laying down a foundation in better understanding of fish genomics.展开更多
A well-known fact about mandarin fish(Siniperca chuatsi)is that females grow faster than males.Mandarin fish that have all-female populations have economic advantages,but traditional neo-male fish are difficult to pro...A well-known fact about mandarin fish(Siniperca chuatsi)is that females grow faster than males.Mandarin fish that have all-female populations have economic advantages,but traditional neo-male fish are difficult to produce.In this study,indirect feeding of 500 mg/kg 17α-methyltestosterone(MT)to XX mandarin fish(60 dpf,days post fertilization)with completely differentiated ovaries induced secondary sex reversal.At 40 days after MT treatment,14.7%of mandarin fish with complete reversal was produced,which showed for the first time that XX mandarin fishes with differentiated ovaries still have potentially bisexual germ cells and the plasticity of sex reversal.In the MT treatment group,there was no significant difference in survival compared to the control group.The body weight of the MT treatment group was significantly lower than that of the control group 40 days after treatment(dat)(P<0.05).During MT treatment,serum 11-KT and T concentrations increased and serum E2 concentration decreased in the MT treatment group,but there was no significant difference in hormone levels between the MT treatment group and the control group.A masculinization of mandarin fish was also confirmed by the expression patterns of sex-specific genes sox9,dmrt1,cyp19a1a and foxl2.A significant amount of sox9 and dmrt1 was expressed in the fish gonads of the MT treatment group,whereas cyp19a1a and foxl2 were minimally expressed in the fish gonads.展开更多
Three different kinds of viruses, the spherical virus SCSV with a diameter of about 280 nm,the rhabdovirus SCRV with a size about 250×120 nm, and the baculovirus SCBV with a size about 200×100 nm, were obser...Three different kinds of viruses, the spherical virus SCSV with a diameter of about 280 nm,the rhabdovirus SCRV with a size about 250×120 nm, and the baculovirus SCBV with a size about 200×100 nm, were observed from the tissues of diseased mandarin fish Siniperca chuatsi with outbreak of infection and acute lethality. This phenomenon implicated that the reason why the epizootic disease of mandarin fish could not be quenched by only one kind of virus vaccine can be explained by the fact that the fish may be infected by different kinds of viruses. Therefore, more attention should be paid to the complexity of virus pathogens in the prevention strategy for mandarin fish diseases.展开更多
The success of initial feeding during the mouth-opening stage is crucial for the survival and growth of fish larvae.Carnivorous mandarin fish(Siniperca chuatsi)face unique challenges in capturing live prey during this...The success of initial feeding during the mouth-opening stage is crucial for the survival and growth of fish larvae.Carnivorous mandarin fish(Siniperca chuatsi)face unique challenges in capturing live prey during this period.The aim of this study was to explore the factors influencing initial feeding in mandarin fish larvae during the mouth-opening stage and validate their functional roles.Transcriptomic analysis of both first feeding and unfed larvae revealed significant changes in the expression of genes related to vision and taste,while fewer changes were observed in genes associated with olfaction.Further sensory inhibition experiments demonstrated that vision and taste are the primary sensory factors driving initial feeding,while olfaction plays a less significant role.Gene set enrichment analysis revealed that the mammalian target of rapamycin(mTOR)is a core regulatory factor in this process.In situ hybridization indicated significant changes in the expression and distribution of mTOR,agouti-related peptide(AgRP),and neuropeptide Y(NPY)before and after feeding.Rapamycin-mediated mTOR inhibition significantly reduced the expression of appetite stimulants(NPY,AgRP,GHRL,and orexin1)and decreased feeding rates.Moreover,we found that inhibiting mTOR signaling also lowered the expression of key digestive enzymes,including pepsin,lipase,and trypsin.In summary,our study identifies vision and taste as the primary sensory factors influencing initial feeding in mandarin fish and clarifies the critical role of the mTOR pathway in coordinating appetite regulation and digestive preparation.These mechanistic insights provide promising targets for improving feeding habit programs for this economically valuable species.展开更多
This study investigated the co-inoculation of Lactobacillus plantarum B8 and Staphylococcus edaphicus F for fermenting Mandarin fish(Siniperca chuatsi)to enhance product quality and safety.On the seventh day of fermen...This study investigated the co-inoculation of Lactobacillus plantarum B8 and Staphylococcus edaphicus F for fermenting Mandarin fish(Siniperca chuatsi)to enhance product quality and safety.On the seventh day of fermentation,the results demonstrated that co-inoculation significantly improved the textural properties of fermented fish and increased the content of umami-and sweet-flavored amino acids.Compared to spontaneous fermentation,co-inoculation reduced total volatile base nitrogen(TVB-N)by 25.53%,thiobarbituric acid reactive substances(TBARS)by 62.82%,and total biogenic amines(BAs)by 27.91%,indicating enhanced freshness,suppressed lipid oxidation,and mitigated safety risks.Furthermore,the co-inoculated samples exhibited significantly lower levels of sulfur-and nitrogen-containing volatile compounds,contributing to a milder flavor profile and superior sensory attributes.Microbial analysis revealed a notable reduction in Vibrio abundance,which correlated with decreased indole and sulfide production,thereby improving odor characteristics.Principal component analysis(PCA)and partial least squares-discriminant analysis(PLS-DA)highlighted the synergistic interactions between microbial communities and their positive impacts on the quality and flavor development of fermented fish.In conclusion,co-inoculation with L.plantarum B8 and S.edaphicus F not only enhances the safety and nutritional value of the products but also improves their sensory properties,making them more appealing to consumers.展开更多
The present experiment investigated the effects of soybean protein concentrated hydrolysate(SPCH)replacing fishmeal(FM)on the growth performance,intestinal health,flesh quality,and nutrient metabolism of mandarin fish...The present experiment investigated the effects of soybean protein concentrated hydrolysate(SPCH)replacing fishmeal(FM)on the growth performance,intestinal health,flesh quality,and nutrient metabolism of mandarin fish(Siniperca chuatsi).Five isonitrogenous and isolipidic diets were designed with different levels of dietary fishmeal substituted with SPCH at 0(FM55),18.70%(FM45),37.16%(FM35),55.40%(FM25),and 73.41%(FM15),respectively.Each diet was fed to three replicate groups of mandarin fish(a total of 450 fish,37.18±0.01 g)twice daily for 8 consecutive weeks,with 30 individuals per tank.The results showed that when the replacement level of SPCH did not exceed 37.16%,no significant effects on the growth of mandarin fish were observed(P>0.05).Histological examination showed significantly enhanced hepatic lipid accumulation,while the mid-intestinal villus length and muscularis thickness reached their maximum in the FM35 group(P<0.05).At the molecular level,SPCH modulated amino acid transport,as evidenced by upregulated expression of key transporter genes(lat1 and snat2)(P<0.05).Meanwhile,SPCH substitution influenced the tor pathway in muscle tissue,as evidenced by the upregulation of related genes(akt1,tor,s6k,and s6)(P<0.05).However,SPCH supplementation linearly upregulated hepatic lipogenic genes(srebp1,dgat1,acc1,acc2,and fas)while downregulating lipid catabolism genes(pparα,aco,and atgl)in a linear manner(P<0.05).Additionally,when SPCH replacement exceeded 37.16%,mid-intestinal expression of inflammation-related genes(rela,mapk13,mapk14,and 5lox)was significantly upregulated(P<0.05).Furthermore,as the replacement level of SPCH increased,muscle hardness,adhesiveness,springiness,chewiness,resilience,and shear force linearly decreased(P<0.05),while gumminess linearly increased(P<0.001).In summary,the study demonstrated that the inclusion of SPCH could effectively reduce the dietary fishmeal content from 55% to 35% without significantly compromising the growth performance,intestinal health,or overall quality of mandarin fish.展开更多
Mandarin fish(Siniperca chuatsi)is an economically important fish in China,and females grow faster than males.Thus,it is critical to obtain neo-male individuals for breeding all-female mandarin fish.In this study,func...Mandarin fish(Siniperca chuatsi)is an economically important fish in China,and females grow faster than males.Thus,it is critical to obtain neo-male individuals for breeding all-female mandarin fish.In this study,functionalized SWCNTs(single-walled carbon nanotubes)were used as a plasmid carrier to immerse juvenile all-female mandarin fish at plasmid concentrations of 10,20,and 40 mg/L.In muscle and gonad tissues,the DNA of pcDNA4.0-amh could be detected in the SWCNTs-pcDNA4.0-amh-40 mg group at 7 days after the immersion bath.Additionally,the transcription of pcDNA4.0-amh could be detected at 7 and 14 days after the immersion bath.At 60,90,and 120 days post-immersion,histological analysis revealed that masculinized gonads could be observed in some fish in the SWCNTs-pcDNA4.0-amh-40 mg and SWCNTs-pcDNA4.0-amhy-40 mg groups.In these masculinized gonads,the expression of the foxl2 and cyp19a1a genes decreased significantly,while the expression of the amh and dmrt1 genes increased significantly.Moreover,a dual-luciferase reporter assay revealed that both amh and amhy could activate the promoter of dmrt1 via the amhrII/smads pathway in vitro.Overall,this study demonstrated that functionalized SWCNTs could act as an effective tool to deliver plasmids into the gonads of fish,and that amh and amhy are both important genes in the regulation of sex determination and differentiation in mandarin fish.展开更多
文摘Serum immunoglobulin from the mandarin fish, or the so called Chinese perch, Siniperca chuatsi (Basilewsky), was successfully purified using affinity chromatography. Heavy and light chains were detected on electrophoresis gel, with molecular weights being estimated at 72 and 29 kDa, respectively. The tetrameric IgM of S. chuatsi was calculated to be 808 kDa. The rabbit polyclonal antisera against the purifed immunoglobulin were developed and tested by Western blot analysis. The antisera reacted strongly with the heavy chains of S. chuatsi immunoglobulin. Humoral immune responses of the mandarin fish can then be examined using the developed polyclonal antibody.
基金Supported by the China Agriculture Research System (No.CARS-46)the National Key R&D Program of China (No.2018YFD0900400)。
文摘Carnivorous fish have poor tolerance to carbohydrate in feed and low utilization rate of carbohydrate.Therefore,the balance of carbohydrate and lipids in the nutrient metabolism of carnivorous fish,the ef fective conversion and utilization of carbohydrate and lipids,and the feedback regulation of feeding are the key links for the e fficient utilization of carnivorous fish feed.Carbohydrate response element binding protein(ChREBP)is a new transcription factor found in recent years in the glucose signaling pathway,and can also participate in feeding regulation.We performed in-vivo and in-vitro experiments to reveal the role of ChREBP in the glucose metabolism and feeding in mandarin fish.The mRNA expression of ChREBP and appetite regulatory factors were measured after intraperitoneal injection of glucose in mandarin fish Siniperca chuatsi and cotransfection with glucose and glucose+siRNA in the hypothalamic cells in mandarin fish.The results reveal that at hour 2 and 4 post intraperitoneal injection with 1 mg/g BW glucose,the blood glucose level of the mandarin fish increased significantly,but food intake decreased significantly,and it also displayed a significantly increased ChREBP mRNA expression levels in liver.At hour 4 post injection,hypothalamic ChREBP mRNA level was significantly increased,whereas the mRNA expression levels of appetite genes neuropeptide Y(npy)and agouti-related peptide(AgRP)were decreased significantly.When the glucose concentration was 40 mmol/L,the expression level of ChREBP mRNA in mandarin fish hypothalamic cells was significantly up-regulated,but the expression level of appetite gene npy mRNA was down-regulated.When siRNA and glucose were co-transfected into mandarin fish brain cells,the expression level of chrebp mRNA was significantly decreased,and the appetite gene npy mRNA was significantly increased.The results indicated that glucose regulated food intake through the modulation of appetite gene npy by ChREBP.
基金Supported by the National Natural Science Foundation of China (No.U0631010),the Government of Guangdong Provincethe National Basic Research Program of China (973 Program) (No. 2009CB118703)
文摘The genomic and cDNA sequences of the CD3γ/δ and CD3ε homologues in the mandarin fish, Siniperca chuatsi, were determined. As in other vertebrate CD3 molecules, the deduced amino acid sequences of mandarin fish CD3γ/δ and CD3ε contained conserved residues and motifs, such as cysteine residues and CXXC and immunoreceptor tyrosine-based activation motifs. However, mandarin fish CD3γ/δ and CD3ε showed some differences to their mammalian counterparts, specifically the absence of a negatively charged residue in the transmembrane region of CD3γ/δ. Additionally, while an N-glycosylation site was present in CD3c, the site was not observed in CD3γ/δ. The CD3γ/δ and CD3ε subunit sequences contain six and five exons, respectively, consistent with homologues from Atlantic salmon, Salmo salar. Phylogenetic analysis also revealed that CD3γ/δ and CD3ε in mandarin fish are closely related to their counterparts in Acanthopterygian fish. Real-time PCR showed CD3γ/δ and CD3ε were expressed mainly in the thymus and spleen in normal healthy fish and, to a lesser extent, in mucosal-associated lymphoid tissues, such as the intestine and gills. When lymphocytes isolated from head kidney were treated with the mitogens phytohemagglutinin, concanavalin, and polyriboinosinic polyribocytidylic acid, mRNA expression levels of CD3γ/δ and CD3ε were significantly elevated within 12 h of treatment. This indicated the presence of T lymphocytes in the head kidney of teleost fish, and also the recognition of mitogens by the lymphocytes. Mandarin fish infected with the bacterial pathogen Flavobacterium columnare also showed an increase in the expression of CD3γ/δ and CD3ε mR_NA, indicating that CD3γ/δ and CD3ε lymphocytes are involved in the immune response of this species.
基金supported by the National Key Research and Development Program of China(2022YFE0203900)Guangdong Key Research and Development Program(2021B0202040002 and 2022B1111030001)+4 种基金China Agriculture Research System(CARS-46)Guangdong Basic and Applied Basic Research Foundation(2021A1515010647)Basic and Applied Basic Research Project of Guangzhou Science and Technology Plan Project(202102020299)Science and Technology Planning Project of Guangdong(2023B1212060023)Guangdong Laboratory for Lingnan Modern Agriculture(NZ2021018)。
文摘The von Hippel-Lindau tumor suppressor protein(VHL),an E3 ubiquitin ligase,functions as a critical regulator of the oxygen-sensing pathway for targeting hypoxia-inducible factors.Recent evidence suggests that mammalian VHL may also be critical to the NF-κB signaling pathway,although the specific molecular mechanisms remain unclear.Herein,the roles of mandarin fish(Siniperca chuatsi)VHL(scVHL)in the NF-κB signaling pathway and mandarin fish ranavirus(MRV)replication were explored.The transcription of scVHL was induced by immune stimulation and MRV infection,indicating a potential role in innate immunity.Dual-luciferase reporter gene assays and reverse transcription quantitative PCR(RT-qPCR)results demonstrated that scVHL evoked and positively regulated the NF-κB signaling pathway.Treatment with NF-κB signaling pathway inhibitors indicated that the role of scVHL may be mediated through scIKKα,scIKKβ,scIκBα,or scp65.Co-immunoprecipitation(Co-IP)analysis identified scIκBαas a novel target protein of scVHL.Moreover,scVHL targeted scIκBαto catalyze the formation of K63-linked polyubiquitin chains to activate the NF-κB signaling pathway.Following MRV infection,NF-κB signaling remained activated,which,in turn,promoted MRV replication.These findings suggest that scVHL not only positively regulates NF-κB but also significantly enhances MRV replication.This study reveals a novel function of scVHL in NF-κB signaling and viral infection in fish.
文摘The heat shock protein 70(HSP70)in fish plays a crucial role in various physiological and environmental stresses.To reveal the mechanisms of the HSP70 gene response to abiotic stress in mandarin fish(Siniperca chuatsi),we analyzed the HSP70 gene family in this species.There are 16 HSP70 genes in mandarin fish,and their protein three-dimensional structures were constructed.The aliphatic index of the 16 mandarin fish HSP70sranges from 73.50(HSPa4b)to 99.46(HSPa13),and all are hydrophilic proteins,with seven of them being unstable proteins.After exposure to chrysene,six HSP70 genes in the intestine,four in the liver,and four in the brain tissues exhibited significant expression changes.These findings reveal that chrysene exposure affects the physiological status of fish,and HSP70 genes may play a role in the chrysene exposure tolerance of the intestine,brain and liver in mandarin fish.
基金supported by the National Natural Science Foundation of China (Grant No. 30972263 and 30771644)the Natural Science Foundation of Hunan Province, China (Grant No. 09JJ6037 and 08jj3064)
文摘To enrich the genomic information of the commercially important fish species, we obtained 5,063 high-quality expressed sequence tags （ESTs） from the muscle cDNA database of the mandarin fish （Siniperca chuatsi）. Clustering analysis yielded 1,625 unique sequences including 443 contigs （from 3,881 EST sequences） and 1,182 sin- gletons. BLASTX searches showed that 959 unique sequences shared homology to proteins in the NCBI non-redundant database. A total of 740 unique sequences were functionally annotated using Gene Ontology. The 1,625 unique sequences were assigned to Kyoto Encyclopedia of Genes and Genomes reference pathways, and the results indicated that transcripts participating in nucleotide metabolism and amino acid metabolism are relatively abundant in S. chuatsi. Meanwhile, we identified 15 genes to be abundantly expressed in muscle of the mandarin fish. These genes are involved in muscle structural formation and regulation of muscle differentiation and development. The most remarkable gene in S. chuatsi is nuclease diphosphate kinase B, which is represented by 449 EST sequences accounting for 8.86% of the total EST sequences. Our work provides a transcript profile expressed in the white muscle of the mandarin fish, laying down a foundation in better understanding of fish genomics.
基金supported by Guangdong Basic and Applied Basic Research Foundation(2019B1515120072)Science and Technology Planning Project of Guangzhou(201904020043)+1 种基金Seed Industry Development Project of Agricultural and Rural Department of Guangdong Province(2022)Innovation Group Project of Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai)(311021006).
文摘A well-known fact about mandarin fish(Siniperca chuatsi)is that females grow faster than males.Mandarin fish that have all-female populations have economic advantages,but traditional neo-male fish are difficult to produce.In this study,indirect feeding of 500 mg/kg 17α-methyltestosterone(MT)to XX mandarin fish(60 dpf,days post fertilization)with completely differentiated ovaries induced secondary sex reversal.At 40 days after MT treatment,14.7%of mandarin fish with complete reversal was produced,which showed for the first time that XX mandarin fishes with differentiated ovaries still have potentially bisexual germ cells and the plasticity of sex reversal.In the MT treatment group,there was no significant difference in survival compared to the control group.The body weight of the MT treatment group was significantly lower than that of the control group 40 days after treatment(dat)(P<0.05).During MT treatment,serum 11-KT and T concentrations increased and serum E2 concentration decreased in the MT treatment group,but there was no significant difference in hormone levels between the MT treatment group and the control group.A masculinization of mandarin fish was also confirmed by the expression patterns of sex-specific genes sox9,dmrt1,cyp19a1a and foxl2.A significant amount of sox9 and dmrt1 was expressed in the fish gonads of the MT treatment group,whereas cyp19a1a and foxl2 were minimally expressed in the fish gonads.
文摘Three different kinds of viruses, the spherical virus SCSV with a diameter of about 280 nm,the rhabdovirus SCRV with a size about 250×120 nm, and the baculovirus SCBV with a size about 200×100 nm, were observed from the tissues of diseased mandarin fish Siniperca chuatsi with outbreak of infection and acute lethality. This phenomenon implicated that the reason why the epizootic disease of mandarin fish could not be quenched by only one kind of virus vaccine can be explained by the fact that the fish may be infected by different kinds of viruses. Therefore, more attention should be paid to the complexity of virus pathogens in the prevention strategy for mandarin fish diseases.
基金funded by the National Key Research and Development Program of China(2024YFD2401502 and 2023YFD2400600).
文摘The success of initial feeding during the mouth-opening stage is crucial for the survival and growth of fish larvae.Carnivorous mandarin fish(Siniperca chuatsi)face unique challenges in capturing live prey during this period.The aim of this study was to explore the factors influencing initial feeding in mandarin fish larvae during the mouth-opening stage and validate their functional roles.Transcriptomic analysis of both first feeding and unfed larvae revealed significant changes in the expression of genes related to vision and taste,while fewer changes were observed in genes associated with olfaction.Further sensory inhibition experiments demonstrated that vision and taste are the primary sensory factors driving initial feeding,while olfaction plays a less significant role.Gene set enrichment analysis revealed that the mammalian target of rapamycin(mTOR)is a core regulatory factor in this process.In situ hybridization indicated significant changes in the expression and distribution of mTOR,agouti-related peptide(AgRP),and neuropeptide Y(NPY)before and after feeding.Rapamycin-mediated mTOR inhibition significantly reduced the expression of appetite stimulants(NPY,AgRP,GHRL,and orexin1)and decreased feeding rates.Moreover,we found that inhibiting mTOR signaling also lowered the expression of key digestive enzymes,including pepsin,lipase,and trypsin.In summary,our study identifies vision and taste as the primary sensory factors influencing initial feeding in mandarin fish and clarifies the critical role of the mTOR pathway in coordinating appetite regulation and digestive preparation.These mechanistic insights provide promising targets for improving feeding habit programs for this economically valuable species.
基金supported by Anhui Provincial Key Research and Development Plan(202104a06020013)Major Science and Technology Special Projects in Anhui Province(2021d06050001)Open Research Project of the Rural Revitalization Collaborative Technical Service Center of Anhui Province(Huangshan University)(XCZXZD2301).
文摘This study investigated the co-inoculation of Lactobacillus plantarum B8 and Staphylococcus edaphicus F for fermenting Mandarin fish(Siniperca chuatsi)to enhance product quality and safety.On the seventh day of fermentation,the results demonstrated that co-inoculation significantly improved the textural properties of fermented fish and increased the content of umami-and sweet-flavored amino acids.Compared to spontaneous fermentation,co-inoculation reduced total volatile base nitrogen(TVB-N)by 25.53%,thiobarbituric acid reactive substances(TBARS)by 62.82%,and total biogenic amines(BAs)by 27.91%,indicating enhanced freshness,suppressed lipid oxidation,and mitigated safety risks.Furthermore,the co-inoculated samples exhibited significantly lower levels of sulfur-and nitrogen-containing volatile compounds,contributing to a milder flavor profile and superior sensory attributes.Microbial analysis revealed a notable reduction in Vibrio abundance,which correlated with decreased indole and sulfide production,thereby improving odor characteristics.Principal component analysis(PCA)and partial least squares-discriminant analysis(PLS-DA)highlighted the synergistic interactions between microbial communities and their positive impacts on the quality and flavor development of fermented fish.In conclusion,co-inoculation with L.plantarum B8 and S.edaphicus F not only enhances the safety and nutritional value of the products but also improves their sensory properties,making them more appealing to consumers.
基金financially supported by the Shanghai Agricultural Science and Technology Innovation Program(K2024-02-0800-12-F00029)Shanghai Sailing Program(24YF2717200).
文摘The present experiment investigated the effects of soybean protein concentrated hydrolysate(SPCH)replacing fishmeal(FM)on the growth performance,intestinal health,flesh quality,and nutrient metabolism of mandarin fish(Siniperca chuatsi).Five isonitrogenous and isolipidic diets were designed with different levels of dietary fishmeal substituted with SPCH at 0(FM55),18.70%(FM45),37.16%(FM35),55.40%(FM25),and 73.41%(FM15),respectively.Each diet was fed to three replicate groups of mandarin fish(a total of 450 fish,37.18±0.01 g)twice daily for 8 consecutive weeks,with 30 individuals per tank.The results showed that when the replacement level of SPCH did not exceed 37.16%,no significant effects on the growth of mandarin fish were observed(P>0.05).Histological examination showed significantly enhanced hepatic lipid accumulation,while the mid-intestinal villus length and muscularis thickness reached their maximum in the FM35 group(P<0.05).At the molecular level,SPCH modulated amino acid transport,as evidenced by upregulated expression of key transporter genes(lat1 and snat2)(P<0.05).Meanwhile,SPCH substitution influenced the tor pathway in muscle tissue,as evidenced by the upregulation of related genes(akt1,tor,s6k,and s6)(P<0.05).However,SPCH supplementation linearly upregulated hepatic lipogenic genes(srebp1,dgat1,acc1,acc2,and fas)while downregulating lipid catabolism genes(pparα,aco,and atgl)in a linear manner(P<0.05).Additionally,when SPCH replacement exceeded 37.16%,mid-intestinal expression of inflammation-related genes(rela,mapk13,mapk14,and 5lox)was significantly upregulated(P<0.05).Furthermore,as the replacement level of SPCH increased,muscle hardness,adhesiveness,springiness,chewiness,resilience,and shear force linearly decreased(P<0.05),while gumminess linearly increased(P<0.001).In summary,the study demonstrated that the inclusion of SPCH could effectively reduce the dietary fishmeal content from 55% to 35% without significantly compromising the growth performance,intestinal health,or overall quality of mandarin fish.
基金supported by the National Key R&D Program of China(grant no.2024YFD2401500,2024YFD2401505)National Natural Science Foundation of China(grant no.32302995+3 种基金the Guangdong Basic and Applied Basic Research Foundation(grant nos.2022A1515110140,2024A1515013176)the Science and Technology Planning Project of Guizhou(grant no.2023-085)the Science and Technology Planning Project of Guangzhou(grant no.2025D04J0094)the Guangzhou Municipal Joint Funding Project of Universities(Institutes)and Enterprises(grant no.2025A03J0022).
文摘Mandarin fish(Siniperca chuatsi)is an economically important fish in China,and females grow faster than males.Thus,it is critical to obtain neo-male individuals for breeding all-female mandarin fish.In this study,functionalized SWCNTs(single-walled carbon nanotubes)were used as a plasmid carrier to immerse juvenile all-female mandarin fish at plasmid concentrations of 10,20,and 40 mg/L.In muscle and gonad tissues,the DNA of pcDNA4.0-amh could be detected in the SWCNTs-pcDNA4.0-amh-40 mg group at 7 days after the immersion bath.Additionally,the transcription of pcDNA4.0-amh could be detected at 7 and 14 days after the immersion bath.At 60,90,and 120 days post-immersion,histological analysis revealed that masculinized gonads could be observed in some fish in the SWCNTs-pcDNA4.0-amh-40 mg and SWCNTs-pcDNA4.0-amhy-40 mg groups.In these masculinized gonads,the expression of the foxl2 and cyp19a1a genes decreased significantly,while the expression of the amh and dmrt1 genes increased significantly.Moreover,a dual-luciferase reporter assay revealed that both amh and amhy could activate the promoter of dmrt1 via the amhrII/smads pathway in vitro.Overall,this study demonstrated that functionalized SWCNTs could act as an effective tool to deliver plasmids into the gonads of fish,and that amh and amhy are both important genes in the regulation of sex determination and differentiation in mandarin fish.
