Objective To investigate the impact of sub-chronic Aluminium-maltolate [Al（mal）s] exposure on the catabolism of amyloid precursor protein （APP） in rats. Methods Forty adult male Sprague-Dawley （SD） rats were ran...Objective To investigate the impact of sub-chronic Aluminium-maltolate [Al（mal）s] exposure on the catabolism of amyloid precursor protein （APP） in rats. Methods Forty adult male Sprague-Dawley （SD） rats were randomly divided into five groups： the control group, the maltolate group （7.56 mg/kg BW）, and the Al（mal）s groups （0.27, 0.54, and 1.08 mg/kg BW, respectively）. Control rats were administered with 0.9% normal saline through intraperitoneal （i.p.） injection. Maltolate and Al（mal）s were administered to the rats also through i.p. injections. Administration was conducted daily for two months. Rat neural behavior was examined using open field tests （OFT）. And the protein expressions and their mRNAs transcription related with APP catabolism were studied using enzyme-linked immunosorbent assay （ELISA） and real-time polymerase chain reaction （RT-PCR）. Results The expressions of APP, 13-site APP cleaving enzyme 1 （BACEI） and presenilin-1 （PSi） proteins and their mRNAs transcription increased gradually with the increase of Al（mal）3 doses （P〈0.05）. The enzyme activity of BACEI in the 0.54 and 1.08 mg/kg Al（mal）s groups increased significantly （P〈0.05）. The expression of 8-amyloid protein （AS） 1-40 gradually decreased while the protein expression of A81-42 increased gradually with the increase of Al（mal）s doses （P〈0.05）. Conclusion Result from our study suggested that one of the possible mechanisms that Al（mal）s can cause neurotoxicity is that Al（mal）s can increase the generation of A81-42 by facilitating the expressions of APP, β-, and γ-secretase.展开更多
The deconstructive reorganization strategy for the synthesis of benzene-containing products from the kojic acid-and maltol-derived alkynes has been recently reported.In this strategy,kojic acid and maltol are analogou...The deconstructive reorganization strategy for the synthesis of benzene-containing products from the kojic acid-and maltol-derived alkynes has been recently reported.In this strategy,kojic acid and maltol are analogous to the"Transformers",which can transform into benzofurans and benzaldehydes via annulation reactions.Under the synthetic standpoint,this deconstructive reorganization strategy features high atom economy,innate scalability and functional group tolerance.In the near future,we believe that this unique method will be widely investigated a nd other novel transformations of kojic acid and maltol will be discovered.展开更多
[Objectives]This study was conducted to establish an uncertainty evaluation method for the determination of ethyl maltol by ultra-high performance liquid chromatograph-mass spectrometer(UPLC-MS).[Methods]A mathematica...[Objectives]This study was conducted to establish an uncertainty evaluation method for the determination of ethyl maltol by ultra-high performance liquid chromatograph-mass spectrometer(UPLC-MS).[Methods]A mathematical model of uncertainty was established by analyzing the method for determining ethyl maltol using UPLC-MS.The sources of uncertainty were analyzed,and the components of uncertainty were calculated to evaluate the expanded uncertainty of the method.[Results]When the content of ethyl maltol in edible vegetable oil was 1657μg/kg,the expanded uncertainty was 22.4μg/kg(K=2,P=95%).[Conclusions]The uncertainty in this evaluation model mainly came from standard solution preparation,sample weighing,dilution of sample to constant volume,standard curve fitting,and repeated measurement.展开更多
基金supported by the National Natural Science Foundation of China (30972512)the Graduate Innovation Fund of Academic Degree Committee Office of the Shanxi Provincial Government (20093014)+1 种基金Doctor Start-up Fund from Shanxi Medical University (B03201209)the College Students Innovation Fund of Shanxi Medical University (2010-25)
文摘Objective To investigate the impact of sub-chronic Aluminium-maltolate [Al（mal）s] exposure on the catabolism of amyloid precursor protein （APP） in rats. Methods Forty adult male Sprague-Dawley （SD） rats were randomly divided into five groups： the control group, the maltolate group （7.56 mg/kg BW）, and the Al（mal）s groups （0.27, 0.54, and 1.08 mg/kg BW, respectively）. Control rats were administered with 0.9% normal saline through intraperitoneal （i.p.） injection. Maltolate and Al（mal）s were administered to the rats also through i.p. injections. Administration was conducted daily for two months. Rat neural behavior was examined using open field tests （OFT）. And the protein expressions and their mRNAs transcription related with APP catabolism were studied using enzyme-linked immunosorbent assay （ELISA） and real-time polymerase chain reaction （RT-PCR）. Results The expressions of APP, 13-site APP cleaving enzyme 1 （BACEI） and presenilin-1 （PSi） proteins and their mRNAs transcription increased gradually with the increase of Al（mal）3 doses （P〈0.05）. The enzyme activity of BACEI in the 0.54 and 1.08 mg/kg Al（mal）s groups increased significantly （P〈0.05）. The expression of 8-amyloid protein （AS） 1-40 gradually decreased while the protein expression of A81-42 increased gradually with the increase of Al（mal）s doses （P〈0.05）. Conclusion Result from our study suggested that one of the possible mechanisms that Al（mal）s can cause neurotoxicity is that Al（mal）s can increase the generation of A81-42 by facilitating the expressions of APP, β-, and γ-secretase.
基金the National Natural Science Foundation of China(Nos.21871053 and 21532001)the Leading Innovative and Entrepreneur Team Introduction Program of Zhejiang(No.2019R01005)。
文摘The deconstructive reorganization strategy for the synthesis of benzene-containing products from the kojic acid-and maltol-derived alkynes has been recently reported.In this strategy,kojic acid and maltol are analogous to the"Transformers",which can transform into benzofurans and benzaldehydes via annulation reactions.Under the synthetic standpoint,this deconstructive reorganization strategy features high atom economy,innate scalability and functional group tolerance.In the near future,we believe that this unique method will be widely investigated a nd other novel transformations of kojic acid and maltol will be discovered.
文摘[Objectives]This study was conducted to establish an uncertainty evaluation method for the determination of ethyl maltol by ultra-high performance liquid chromatograph-mass spectrometer(UPLC-MS).[Methods]A mathematical model of uncertainty was established by analyzing the method for determining ethyl maltol using UPLC-MS.The sources of uncertainty were analyzed,and the components of uncertainty were calculated to evaluate the expanded uncertainty of the method.[Results]When the content of ethyl maltol in edible vegetable oil was 1657μg/kg,the expanded uncertainty was 22.4μg/kg(K=2,P=95%).[Conclusions]The uncertainty in this evaluation model mainly came from standard solution preparation,sample weighing,dilution of sample to constant volume,standard curve fitting,and repeated measurement.
