目的:探讨Th17细胞、血清白细胞介素-17 (IL-17)、基质金属蛋白酶(MMP2)在评估胃癌(GC)淋巴结转移中的临床应用价值。方法:选取2023年1月~2024年10月入住于天津市武清中医院的36例胃癌患者(GC组)及38例胃良性病变患者(对照组),采用流式...目的:探讨Th17细胞、血清白细胞介素-17 (IL-17)、基质金属蛋白酶(MMP2)在评估胃癌(GC)淋巴结转移中的临床应用价值。方法:选取2023年1月~2024年10月入住于天津市武清中医院的36例胃癌患者(GC组)及38例胃良性病变患者(对照组),采用流式细胞术检测各组外周血Th17细胞水平,采用酶联免疫吸附法(ELISA)测定血清中IL-17和MMP2水平,评估这三个检测指标在GC淋巴结转移中的应用价值及三者联合检测的诊断效能。结果:与对照组对比,GC组患者外周血Th17细胞水平明显增高,血清IL-17和MMP2水平也明显较高,差异均有统计学意义(均p Objective: To explore the clinical application value of Th17 cells, serum Interleukin-17 (IL-17), and Matrix Metalloproteinases (MMP2) in assessing the lymph node metastasis of Gastric Cancer (GC). Methods: 36 GC patients (GC group) and 38 patients with benign gastric lesions (control group) admitted to Tianjin Wuqing Hospital of Traditional Chinese Medicine from January 2023 to October 2024 were selected. Flow cytometry was used to detect peripheral blood Th17 cell amount in each group, and Enzyme-Linked Immunosorbent Assay (ELISA) was used to measure IL-17 and MMP2 levels in the serum. The application value of these three detection indicators in the lymph node metastasis of GC, as well as the diagnostic efficacy of their combined detection, were evaluated. Results: Compared with control group, patients in the GC group showed a significant increase in Th17 cell levels in peripheral blood and prominently higher serum IL-17 and MMP2 levels, with statistical significance (all p < 0.05). In the GC group, the amount of Th17 cells, serum IL-17, and MMP-2 was significantly higher in patients with lymph node metastasis compared to those without metastasis, and the differences were statistically significant (all p < 0.05). The accuracy, specificity, and sensitivity of the combined detection of Th17, IL-17, and MMP2 were 95.82%, 94.21% and 97.69%, respectively. Compared with single detection, they were significantly higher, and the differences were statistically significant (all p < 0.05). Conclusion: Th17 cells, IL-17, and MMP2 are associated with lymph node metastasis in GC, and their combined detection can help in the differential diagnosis of GC.展开更多
Background:The purpose of the study was to investigate the active ingredients and potential biochemical mechanisms of Juanbi capsule in knee osteoarthritis based on network pharmacology,molecular docking and animal ex...Background:The purpose of the study was to investigate the active ingredients and potential biochemical mechanisms of Juanbi capsule in knee osteoarthritis based on network pharmacology,molecular docking and animal experiments.Methods:Chemical components for each drug in the Juanbi capsule were obtained from Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform,while the target proteins for knee osteoarthritis were retrieved from the Drugbank,GeneCards,and OMIM databases.The study compared information on knee osteoarthritis and the targets of drugs to identify common elements.The data was imported into the STRING platform to generate a protein-protein interaction network diagram.Subsequently,a“component-target”network diagram was created using the screened drug components and target information with Cytoscape software.Common targets were imported into Metascape for GO function and KEGG pathway enrichment analysis.AutoDockTools was utilized to predict the molecular docking of the primary chemical components and core targets.Ultimately,the key targets were validated through animal experiments.Results:Juanbi capsule ameliorated Knee osteoarthritis mainly by affecting tumor necrosis factor,interleukin1β,MMP9,PTGS2,VEGFA,TP53,and other cytokines through quercetin,kaempferol,andβ-sitosterol.The drug also influenced the AGE-RAGE,interleukin-17,tumor necrosis factor,Relaxin,and NF-κB signaling pathways.The network pharmacology analysis results were further validated in animal experiments.The results indicated that Juanbi capsule could decrease the levels of tumor necrosis factor-αand interleukin-1βin the serum and synovial fluid of knee osteoarthritis rats and also down-regulate the expression levels of MMP9 and PTGS2 proteins in the articular cartilage.Conclusion:Juanbi capsule may improve the knee bone microstructure and reduce the expression of inflammatory factors of knee osteoarthritis via multiple targets and multiple signaling pathways.展开更多
文摘目的:探讨Th17细胞、血清白细胞介素-17 (IL-17)、基质金属蛋白酶(MMP2)在评估胃癌(GC)淋巴结转移中的临床应用价值。方法:选取2023年1月~2024年10月入住于天津市武清中医院的36例胃癌患者(GC组)及38例胃良性病变患者(对照组),采用流式细胞术检测各组外周血Th17细胞水平,采用酶联免疫吸附法(ELISA)测定血清中IL-17和MMP2水平,评估这三个检测指标在GC淋巴结转移中的应用价值及三者联合检测的诊断效能。结果:与对照组对比,GC组患者外周血Th17细胞水平明显增高,血清IL-17和MMP2水平也明显较高,差异均有统计学意义(均p Objective: To explore the clinical application value of Th17 cells, serum Interleukin-17 (IL-17), and Matrix Metalloproteinases (MMP2) in assessing the lymph node metastasis of Gastric Cancer (GC). Methods: 36 GC patients (GC group) and 38 patients with benign gastric lesions (control group) admitted to Tianjin Wuqing Hospital of Traditional Chinese Medicine from January 2023 to October 2024 were selected. Flow cytometry was used to detect peripheral blood Th17 cell amount in each group, and Enzyme-Linked Immunosorbent Assay (ELISA) was used to measure IL-17 and MMP2 levels in the serum. The application value of these three detection indicators in the lymph node metastasis of GC, as well as the diagnostic efficacy of their combined detection, were evaluated. Results: Compared with control group, patients in the GC group showed a significant increase in Th17 cell levels in peripheral blood and prominently higher serum IL-17 and MMP2 levels, with statistical significance (all p < 0.05). In the GC group, the amount of Th17 cells, serum IL-17, and MMP-2 was significantly higher in patients with lymph node metastasis compared to those without metastasis, and the differences were statistically significant (all p < 0.05). The accuracy, specificity, and sensitivity of the combined detection of Th17, IL-17, and MMP2 were 95.82%, 94.21% and 97.69%, respectively. Compared with single detection, they were significantly higher, and the differences were statistically significant (all p < 0.05). Conclusion: Th17 cells, IL-17, and MMP2 are associated with lymph node metastasis in GC, and their combined detection can help in the differential diagnosis of GC.
基金funding from the Basic Research Project of the Education Department of Shaanxi Province(21JC010,21JP035)the Young and Middle-Aged Scientific Research and Innovation Team of the Shaanxi Provincial Administration of Traditional Chinese Medicine(2022SLRHLJ001)the 2023 Central Financial Transfer Payment Local Project“Innovation and Improvement of Five Types of Hospital Preparations,Such as Roumudan Granules”.
文摘Background:The purpose of the study was to investigate the active ingredients and potential biochemical mechanisms of Juanbi capsule in knee osteoarthritis based on network pharmacology,molecular docking and animal experiments.Methods:Chemical components for each drug in the Juanbi capsule were obtained from Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform,while the target proteins for knee osteoarthritis were retrieved from the Drugbank,GeneCards,and OMIM databases.The study compared information on knee osteoarthritis and the targets of drugs to identify common elements.The data was imported into the STRING platform to generate a protein-protein interaction network diagram.Subsequently,a“component-target”network diagram was created using the screened drug components and target information with Cytoscape software.Common targets were imported into Metascape for GO function and KEGG pathway enrichment analysis.AutoDockTools was utilized to predict the molecular docking of the primary chemical components and core targets.Ultimately,the key targets were validated through animal experiments.Results:Juanbi capsule ameliorated Knee osteoarthritis mainly by affecting tumor necrosis factor,interleukin1β,MMP9,PTGS2,VEGFA,TP53,and other cytokines through quercetin,kaempferol,andβ-sitosterol.The drug also influenced the AGE-RAGE,interleukin-17,tumor necrosis factor,Relaxin,and NF-κB signaling pathways.The network pharmacology analysis results were further validated in animal experiments.The results indicated that Juanbi capsule could decrease the levels of tumor necrosis factor-αand interleukin-1βin the serum and synovial fluid of knee osteoarthritis rats and also down-regulate the expression levels of MMP9 and PTGS2 proteins in the articular cartilage.Conclusion:Juanbi capsule may improve the knee bone microstructure and reduce the expression of inflammatory factors of knee osteoarthritis via multiple targets and multiple signaling pathways.
