摘要
目的 探讨β-榄香酮酸(β-EA)对结肠癌细胞增殖和侵袭的抑制作用及其是否通过PI3K/AKT/mTOR信号通路实现。方法 使用MTT比色法和集落形成实验验证β-榄香酮酸对结肠癌细胞增殖的影响;Transwell小室侵袭实验检测β-榄香酮酸对细胞侵袭能力的影响;Western blot检测处理后细胞中PI3K/AKT/mTOR通路蛋白变化。结果 MTT实验表明β-榄香酮酸有效抑制结肠癌细胞系HCT8和HCT116的增殖,呈剂量依赖性。集落形成实验显示其对细胞增殖有抑制作用。Transwell实验显示β-榄香酮酸抑制细胞侵袭能力。Western blot显示β-榄香酮酸处理后,凋亡相关蛋cleaved-caspase 3、cleaved-caspase 9、Bax表达增加,Bcl-2表达减少;侵袭相关蛋白vimentin、Snail、MMP2、MMP9表达下调。同时,β-榄香酮酸减少p-PI3K、p-Akt、p-mTOR表达,加入PI3K抑制剂LY294002后,下调作用更显著。结论 β-榄香酮酸可能通过PI3K/AKT/mTOR信号通路抑制结肠癌细胞系HCT8和HCT116增殖及侵袭能力,可能成为治疗结肠癌的新型药物。
Objective To investigate the inhibitory effect ofβ-elemonic acid(β-EA)on the proliferation and invasion of colon cancer cells and the underlying mechanisms through PI3K/AKT/mTOR signaling pathway.Methods The effects ofβ-EA on colon cancer cell proliferation were evaluated using the MTT assay and colony formation assay.Transwell invasion assay were used to assess the impact ofβ-EA on invasion.Western blot analysis was conducted to detect changes in PI3K/AKT/mTOR pathway proteins after treatment.Results MTT assay showed thatβ-EA effectively inhibited the proliferation of colon cancer HCT8 and HCT116 cells in a dose-dependent manner.The colony formation assay confirmed its inhibitory effect on cell proliferation.Transwell invasion assays demonstrated thatβ-elemonic acid reduced the invasion abilities of the cells.Western blot analysis revealed increased expression of apoptosis-related proteins cleaved-caspase 3,cleaved-caspase 9,and Bax,while Bcl-2 expression was decreased.Invasion-related proteins vimentin,snail,MMP2,and MMP9 were downregulated after treatment.Additionally,β-EA reduced the levels of p-PI3K,p-Akt,and p-mTOR,and these reductions were more pronounced after the addition of the PI3K inhibitor LY294002.Conclusionsβ-EA may inhibit proliferation and invasion in colon cancer cell lines HCT8 and HCT116 through PI3K/AKT/mTOR signaling pathway,and potentially be transformed as a novel therapeutic agent for colon cancer.
作者
孙婷
邹腾
杨轶嵩
刘双萍
任欣
王丹
SUN Ting;ZOU Teng;YANG Yisong;LIU Shuangping;REN Xin;WANG Dan(Department of Laboratory Medicine,Medical College,Dalian University,Dalian 116622;Clinical Laboratory,Baoding No.1 Central Hospital,Baoding 071000,China)
出处
《基础医学与临床》
2025年第6期748-754,共7页
Basic and Clinical Medicine
基金
辽宁省创新人才支持计划(辽教函2020389)。
