Spider mites are significant pests in agricultural production.The increasing resistance of spider mites,along with environmental pollution and ecological imbalance caused by their control,is primarily attributed to th...Spider mites are significant pests in agricultural production.The increasing resistance of spider mites,along with environmental pollution and ecological imbalance caused by their control,is primarily attributed to the long-term use of chemical acaricides in agriculture.In contrast,bioactive substances of biological origin offer advantages such as wide availability,environmental friendliness,and low tendency to induce resistance,making them a research hotspot for spider mite control.This review summarizes recent advances in the use of plant-derived active compounds(exemplified by extracts from Veratrum rhizomes),RNA interference(RNAi)technology,and microorganism-derived active substances for controlling spider mites.These bioactive agents exert acaricidal effects by disrupting the nervous system,interfering with metabolic processes,or silencing key genes in mites,demonstrating favorable efficacy and considerable potential for development.However,challenges remain,including poor environmental stability,slow action,high production costs,and insufficient understanding of their effects on non-target organisms.Therefore,future research should focus on the screening and development of novel bioactive substances of biological origin,elucidation of their mechanisms of action,optimization of formulation technologies,and assessment of their ecological safety.These efforts will provide valuable insights for promoting the advancement of bioactive substances and supporting sustainable agricultural development.展开更多
The two-spotted spider mite,Tetranychus urticae Koch,is one of the most harmful pests in many agroecosystems worldwide.To effectively manage this pest,there is an urgent need to develop novel bio-active acaricides tha...The two-spotted spider mite,Tetranychus urticae Koch,is one of the most harmful pests in many agroecosystems worldwide.To effectively manage this pest,there is an urgent need to develop novel bio-active acaricides that support integrated pest management strategies targeting T.urticae.In this study,we explored the acaricidal effects of xenocoumacin 1 (Xcn1) on T.urticae and its predator Neoseiulus californicus using the highly puri?ed compound.Xcn1 was extracted and purified from the cell-free supernatant of the Xenorhabdus nematophila CB6 mutant constructed by the easy promoter activated compound identi?cation (easyPACId) method.When the concentration of Xcn1 exceeded 100μg mL~(–1),the survival rate of spider mite adults declined to below 40%and the fecundity was decreased by 80%at six days post-application.At concentrations of 25 and 50μg mL~(–1),Xcn1 signi?cantly impeded spider mite development by inhibiting the molt.However,neither concentration had any adverse effects on the survival or reproduction of the predatory mite N.californicus.The results from laboratory and semi-?eld experiments consistently demonstrated the effectiveness of the antimicrobial metabolite Xcn1 in controlling pest mites at both the molecular and physiological levels.Our study offers a promising possibility that combines the compatible biocontrol agents of Xcn1 and predatory mites for integrated pest mite control.展开更多
One of the fundamental questions in community ecology is whether communities are random or formed by deterministic mechanisms. Although many efforts have been made to verify non-randomness in community structure, litt...One of the fundamental questions in community ecology is whether communities are random or formed by deterministic mechanisms. Although many efforts have been made to verify non-randomness in community structure, little is known with regard to co-occurrence patterns in above-ground and below-ground communities. In this paper, we used a null model to test non-randomness in the structure of the above-ground and below-ground mite communities in farmland of the Sanjiang Plain, Northeast China. Then, we used four tests for non-randomness to recognize species pairs that would be demonstrated as significantly aggregated or segregated co-occurrences of the above-ground and below-ground mite communities. The pattern of the above-ground mite commu- nity was significantly non-random in October, suggesting species segregation and hence interspecific competition. Additionally, species co-occurrence patterns did not differ from randomness in the above-ground mite community in August or in below-ground mite com- munities in August and October. Only one significant species pair was detected in the above-ground mite community in August, while no significant species pairs were recognized in the above-ground mite community in October or in the below-ground mite communities in August and October. The results indicate that non-randomness and significant species pairs may not be the general rule in the above-ground and below-ground mite communities in farmland of the Sanjiang Plain at the fine scale.展开更多
利用生物信息学方法,首次在人气管普孢子虫（Trachipleistophora hominis）基因组内鉴定到9个MITEs家族ThME1ThME9,共123个拷贝,MITEs转座子的长度均小于600bp。根据靶位点重复序列（Target site duplication,TSD）的不同,将ThME1归属于...利用生物信息学方法,首次在人气管普孢子虫（Trachipleistophora hominis）基因组内鉴定到9个MITEs家族ThME1ThME9,共123个拷贝,MITEs转座子的长度均小于600bp。根据靶位点重复序列（Target site duplication,TSD）的不同,将ThME1归属于Tc1/Mariner超家族,ThME2和ThME3归属于PIF/Harbinger超家族,ThME5和ThME6归属于CACTA超家族（超家族的名字用正体较好,下同）,其余家族归为新家族。分析发现,人气管普孢子虫中的所有MITEs家族的吉布斯自由能均小于0,表明MITEs家族具有形成二级结构的潜能,有利于该家族在基因组上转座。人气管普孢子虫MITEs家族在基因组的插入时间估计在0-800万年内,而且这种插入在基因组中是随机的,没有位置偏向性,并发现2个MITEs转座子拷贝插入到基因编码区内部。上述结果为进一步研究微孢子虫MITEs转座子的起源以及功能奠定了基础。展开更多
This paper deals with the validation and theoretical analyses of a simple method for calculating the intrinsic rate of increase, rm, of aphids and mites. This method does not require a detailed fecundity table data. T...This paper deals with the validation and theoretical analyses of a simple method for calculating the intrinsic rate of increase, rm, of aphids and mites. This method does not require a detailed fecundity table data. The value of rm can be estimated by the simple equation: rm=0.74ln (Md)/d, where d is the time from birth to first reproduction; Md is the number of female offspring produced per original female in 2d. This method was developed by Wyatt and White. We reached the following conclusions from our study: When the parameter, Md is less than 1, this equation is not appropriate for estimating rm of populations; When the parameter, Md is larger than 1 and about 70% or more of the reproductive contribution to the rm is achieved in 2d, the equation is appropriate for calculatng the rm of aphids and mites.As Md decreases, the required reproductive contribution to the rm in 2d corresponding to the constant 0.74 will increase. However, whether 70% or more of reproductive contribution to rm is achieved in 2d still is a problem for whole taxa of aphids and mites. Therefore, further siudy on reproductive distribution will be required for practical application of this method.展开更多
文摘Spider mites are significant pests in agricultural production.The increasing resistance of spider mites,along with environmental pollution and ecological imbalance caused by their control,is primarily attributed to the long-term use of chemical acaricides in agriculture.In contrast,bioactive substances of biological origin offer advantages such as wide availability,environmental friendliness,and low tendency to induce resistance,making them a research hotspot for spider mite control.This review summarizes recent advances in the use of plant-derived active compounds(exemplified by extracts from Veratrum rhizomes),RNA interference(RNAi)technology,and microorganism-derived active substances for controlling spider mites.These bioactive agents exert acaricidal effects by disrupting the nervous system,interfering with metabolic processes,or silencing key genes in mites,demonstrating favorable efficacy and considerable potential for development.However,challenges remain,including poor environmental stability,slow action,high production costs,and insufficient understanding of their effects on non-target organisms.Therefore,future research should focus on the screening and development of novel bioactive substances of biological origin,elucidation of their mechanisms of action,optimization of formulation technologies,and assessment of their ecological safety.These efforts will provide valuable insights for promoting the advancement of bioactive substances and supporting sustainable agricultural development.
基金supported by the National Natural Science Foundation of China(32070402)the Beijing Natural Science Foundation,China(6222052)。
文摘The two-spotted spider mite,Tetranychus urticae Koch,is one of the most harmful pests in many agroecosystems worldwide.To effectively manage this pest,there is an urgent need to develop novel bio-active acaricides that support integrated pest management strategies targeting T.urticae.In this study,we explored the acaricidal effects of xenocoumacin 1 (Xcn1) on T.urticae and its predator Neoseiulus californicus using the highly puri?ed compound.Xcn1 was extracted and purified from the cell-free supernatant of the Xenorhabdus nematophila CB6 mutant constructed by the easy promoter activated compound identi?cation (easyPACId) method.When the concentration of Xcn1 exceeded 100μg mL~(–1),the survival rate of spider mite adults declined to below 40%and the fecundity was decreased by 80%at six days post-application.At concentrations of 25 and 50μg mL~(–1),Xcn1 signi?cantly impeded spider mite development by inhibiting the molt.However,neither concentration had any adverse effects on the survival or reproduction of the predatory mite N.californicus.The results from laboratory and semi-?eld experiments consistently demonstrated the effectiveness of the antimicrobial metabolite Xcn1 in controlling pest mites at both the molecular and physiological levels.Our study offers a promising possibility that combines the compatible biocontrol agents of Xcn1 and predatory mites for integrated pest mite control.
基金Under the auspices of National Natural Science Foundation of China(No.41101049,40601047,41371072,31101617,41171047)China Postdoctoral Science Foundation(No.2012M511361)+2 种基金Excellent Youth Scholars of Northeast Institute of Geography and Agroecology,Chinese Academy of Sciences(No.DLSYQ2012004)Fund for Distinguished Young Scholar of Harbin Normal University(No.KGB201204)Scientific Innovation Project for Doctoral Candidate of Harbin Normal University(No.HSDBSCX2012-07)
文摘One of the fundamental questions in community ecology is whether communities are random or formed by deterministic mechanisms. Although many efforts have been made to verify non-randomness in community structure, little is known with regard to co-occurrence patterns in above-ground and below-ground communities. In this paper, we used a null model to test non-randomness in the structure of the above-ground and below-ground mite communities in farmland of the Sanjiang Plain, Northeast China. Then, we used four tests for non-randomness to recognize species pairs that would be demonstrated as significantly aggregated or segregated co-occurrences of the above-ground and below-ground mite communities. The pattern of the above-ground mite commu- nity was significantly non-random in October, suggesting species segregation and hence interspecific competition. Additionally, species co-occurrence patterns did not differ from randomness in the above-ground mite community in August or in below-ground mite com- munities in August and October. Only one significant species pair was detected in the above-ground mite community in August, while no significant species pairs were recognized in the above-ground mite community in October or in the below-ground mite communities in August and October. The results indicate that non-randomness and significant species pairs may not be the general rule in the above-ground and below-ground mite communities in farmland of the Sanjiang Plain at the fine scale.
文摘利用生物信息学方法,首次在人气管普孢子虫（Trachipleistophora hominis）基因组内鉴定到9个MITEs家族ThME1ThME9,共123个拷贝,MITEs转座子的长度均小于600bp。根据靶位点重复序列（Target site duplication,TSD）的不同,将ThME1归属于Tc1/Mariner超家族,ThME2和ThME3归属于PIF/Harbinger超家族,ThME5和ThME6归属于CACTA超家族（超家族的名字用正体较好,下同）,其余家族归为新家族。分析发现,人气管普孢子虫中的所有MITEs家族的吉布斯自由能均小于0,表明MITEs家族具有形成二级结构的潜能,有利于该家族在基因组上转座。人气管普孢子虫MITEs家族在基因组的插入时间估计在0-800万年内,而且这种插入在基因组中是随机的,没有位置偏向性,并发现2个MITEs转座子拷贝插入到基因编码区内部。上述结果为进一步研究微孢子虫MITEs转座子的起源以及功能奠定了基础。
文摘This paper deals with the validation and theoretical analyses of a simple method for calculating the intrinsic rate of increase, rm, of aphids and mites. This method does not require a detailed fecundity table data. The value of rm can be estimated by the simple equation: rm=0.74ln (Md)/d, where d is the time from birth to first reproduction; Md is the number of female offspring produced per original female in 2d. This method was developed by Wyatt and White. We reached the following conclusions from our study: When the parameter, Md is less than 1, this equation is not appropriate for estimating rm of populations; When the parameter, Md is larger than 1 and about 70% or more of the reproductive contribution to the rm is achieved in 2d, the equation is appropriate for calculatng the rm of aphids and mites.As Md decreases, the required reproductive contribution to the rm in 2d corresponding to the constant 0.74 will increase. However, whether 70% or more of reproductive contribution to rm is achieved in 2d still is a problem for whole taxa of aphids and mites. Therefore, further siudy on reproductive distribution will be required for practical application of this method.
