Many of us have the feeling: you step out in a T-shirt on a summer day, and within twenty minutes, your underarms feel damp. There are sweat marks on the clothes, and they are clearly visible. While many debate the me...Many of us have the feeling: you step out in a T-shirt on a summer day, and within twenty minutes, your underarms feel damp. There are sweat marks on the clothes, and they are clearly visible. While many debate the merits of polyester fabric with cool-touch feelingversus cotton as temperatures soar, the apparel world is quietly experiencing a "merino wool trend." A lot of major brands are launching merino wool short-sleeve tees.展开更多
As a member of the Frizzled family, Frizzled3 (FZD3) is a receptor of the canonical Wnt signaling pathway and plays a vital role in mammalian hair follicle developmental processes. However, its effects on wool traits ...As a member of the Frizzled family, Frizzled3 (FZD3) is a receptor of the canonical Wnt signaling pathway and plays a vital role in mammalian hair follicle developmental processes. However, its effects on wool traits are not clear. The objectives of this study were to identify the single nucleotide polymorphisms (SNPs) and the expression patterns of FZD3 gene, and then to determine whether it affected wool traits of Chinese Merino sheep (Xinjiang Type) or not. PCR-single stranded conformational polymorphism (PCR-SSCP) and sequencing were used to identify mutation loci, and general linear model (GLM) with SAS 9.1 was used for the association analysis between wool traits and SNPs. Quantitative real-time PCR (qRT-PCR) was used to investigate FZD3 gene expression levels. The results showed that six exons of FZD3 gene were amplified and two mutation loci were identified in exon 1 (NC_019459.2: g.101771685 T>C (SNP1)) and exon 3 (NC_019459.2: g.101810848, A>C (SNP2)), respectively. Association analysis showed that SNP1 was significantly associated with mean fiber diameter (MFD)(P=0.04) and live weight (LW)(P=0.0004), SNP2 was significantly associated with greasy fleece weight (GFW)(P=0.04). The expression level of FZD3 gene in skin tissues of the superfine wool (SF) group was significantly lower (P<0.05) than that of the fine wool (F) group. Moreover, it had a higher expression level (P<0.01) in skin tissues than in other tissues of Chinese Merino ewes. While, its expression level had a fluctuant expression in skin tissues at different developmental stages of embryos and born lambs, with the highest expression levels (P<0.01) at the 65th day of embryos. Our study revealed the genetic relationship between FZD3 variants and wool traits and two identified SNPs might serve as potential and valuable genetic markers for sheep breeding and lay a molecular genetic foundation for sheep marker-assisted selection (MAS).展开更多
[Objective] The paper was to study the adaptability of Australian Merino sheep in the alpine pastoral areas. [Method]Through introduction of Australian Merino sheep for hybridizing with Gansu alpine fine wool sheep,th...[Objective] The paper was to study the adaptability of Australian Merino sheep in the alpine pastoral areas. [Method]Through introduction of Australian Merino sheep for hybridizing with Gansu alpine fine wool sheep,the improvement effect of offspring was observed. [Result]The introduced Australian Merino sheep had good adaptability in the alpine pastoral areas with good hair quality,strong cold resistance and disease resistance,and they could grow and reproduce healthily in the alpine pastoral areas. The application effect of the ram was outstanding,showing large breeding amount,high conception rate and survival rate.[Conclusion]The improvement effect of offspring of Australian Merino sheep and Gansu alpine fine wool sheep was significant,and the growth performance was improved greatly.展开更多
文摘Many of us have the feeling: you step out in a T-shirt on a summer day, and within twenty minutes, your underarms feel damp. There are sweat marks on the clothes, and they are clearly visible. While many debate the merits of polyester fabric with cool-touch feelingversus cotton as temperatures soar, the apparel world is quietly experiencing a "merino wool trend." A lot of major brands are launching merino wool short-sleeve tees.
基金supported by the National Natural Science Foundation of China (31360543 and 31760655)the earmarked fund for the China Agriculture Research System (CARS-39)+1 种基金the Open Project of Key Laboratory of Genetics Breeding and Reproduction of Xinjiang Cashmere and Wool Sheep, China (2016D03017)the Postdoctoral Science Foundation, China (2017M623287)
文摘As a member of the Frizzled family, Frizzled3 (FZD3) is a receptor of the canonical Wnt signaling pathway and plays a vital role in mammalian hair follicle developmental processes. However, its effects on wool traits are not clear. The objectives of this study were to identify the single nucleotide polymorphisms (SNPs) and the expression patterns of FZD3 gene, and then to determine whether it affected wool traits of Chinese Merino sheep (Xinjiang Type) or not. PCR-single stranded conformational polymorphism (PCR-SSCP) and sequencing were used to identify mutation loci, and general linear model (GLM) with SAS 9.1 was used for the association analysis between wool traits and SNPs. Quantitative real-time PCR (qRT-PCR) was used to investigate FZD3 gene expression levels. The results showed that six exons of FZD3 gene were amplified and two mutation loci were identified in exon 1 (NC_019459.2: g.101771685 T>C (SNP1)) and exon 3 (NC_019459.2: g.101810848, A>C (SNP2)), respectively. Association analysis showed that SNP1 was significantly associated with mean fiber diameter (MFD)(P=0.04) and live weight (LW)(P=0.0004), SNP2 was significantly associated with greasy fleece weight (GFW)(P=0.04). The expression level of FZD3 gene in skin tissues of the superfine wool (SF) group was significantly lower (P<0.05) than that of the fine wool (F) group. Moreover, it had a higher expression level (P<0.01) in skin tissues than in other tissues of Chinese Merino ewes. While, its expression level had a fluctuant expression in skin tissues at different developmental stages of embryos and born lambs, with the highest expression levels (P<0.01) at the 65th day of embryos. Our study revealed the genetic relationship between FZD3 variants and wool traits and two identified SNPs might serve as potential and valuable genetic markers for sheep breeding and lay a molecular genetic foundation for sheep marker-assisted selection (MAS).
基金Supported by Special Fund for Construction of National Wool-purpose Sheep Industry Research System(CARS-40-33)
文摘[Objective] The paper was to study the adaptability of Australian Merino sheep in the alpine pastoral areas. [Method]Through introduction of Australian Merino sheep for hybridizing with Gansu alpine fine wool sheep,the improvement effect of offspring was observed. [Result]The introduced Australian Merino sheep had good adaptability in the alpine pastoral areas with good hair quality,strong cold resistance and disease resistance,and they could grow and reproduce healthily in the alpine pastoral areas. The application effect of the ram was outstanding,showing large breeding amount,high conception rate and survival rate.[Conclusion]The improvement effect of offspring of Australian Merino sheep and Gansu alpine fine wool sheep was significant,and the growth performance was improved greatly.
