A new Megaselia species： Megaselia angustirostris Fang ＆ Liu, sp. nov., is described and illustrated and two species of the genus, M. nigra （Meigen） and M. albicaudata （Wood）, are reported for the first time fro...A new Megaselia species： Megaselia angustirostris Fang ＆ Liu, sp. nov., is described and illustrated and two species of the genus, M. nigra （Meigen） and M. albicaudata （Wood）, are reported for the first time from China. The type specimens are deposited in College of Biological and Environmental Engineering, Shenyang University.展开更多
Megaselia wuzhiensis, sp. nov., from Mt. Wuzhi, Hainan Province is described. It is similar to M. atricornis Beyer, but differs in Costal Ratios and hypopygium.
Two new species of the genus Megaselia, M. pennisetalis sp. nov. and M. tarsocrassa sp. nov., are described and illustrated. The type specimens are deposited in Liaoning Key Laboratory of Urban Integrated Pest Managem...Two new species of the genus Megaselia, M. pennisetalis sp. nov. and M. tarsocrassa sp. nov., are described and illustrated. The type specimens are deposited in Liaoning Key Laboratory of Urban Integrated Pest Management and Ecological Security, Shenyang University.展开更多
目的:建立应用DNA条形码技术鉴定蛆症异蚤蝇的方法。方法:分别提取在口岸检疫中截获的双翅目幼虫和成虫的基因组DNA,利用节肢动物线粒体细胞色素氧化酶I(COI)的通用引物进行DNA扩增。PCR产物直接测序,测序引物同扩增引物,得到清晰可读的...目的:建立应用DNA条形码技术鉴定蛆症异蚤蝇的方法。方法:分别提取在口岸检疫中截获的双翅目幼虫和成虫的基因组DNA,利用节肢动物线粒体细胞色素氧化酶I(COI)的通用引物进行DNA扩增。PCR产物直接测序,测序引物同扩增引物,得到清晰可读的665 bp COI的DNA片段碱基序列。与GenBank中相近的序列进行比较,并用ClustalW2在线建立NJ系统发育树。结果:成虫和幼虫COI的DNA序列100%相同,与GenBank中公布的长度为559 bp的蛆症异蚤蝇的COI相应片段相似性高达98.9%,与同属其他种类的可比片段的DNA序列相似性则低于90%。结论:根据结果可以判断所截获的双翅目昆虫为蛆症异蚤蝇。本文的研究再一次证明了DNA条形码技术可以直接应用于昆虫种类的鉴定。展开更多
在双叉犀金龟人工饲养过程中,连续2年出现了的拟寄生现象,对拟寄生的寄主种类进行鉴定。形态鉴定:寄主的幼虫、蛹和成虫均符合蛆症异蚤蝇(Megaselia scalaris)的形态特征;DNA条形码鉴定:扩增的842 bp碱基含量分别为A:29.6%,T:37.9%,G:16...在双叉犀金龟人工饲养过程中,连续2年出现了的拟寄生现象,对拟寄生的寄主种类进行鉴定。形态鉴定:寄主的幼虫、蛹和成虫均符合蛆症异蚤蝇(Megaselia scalaris)的形态特征;DNA条形码鉴定:扩增的842 bp碱基含量分别为A:29.6%,T:37.9%,G:16.2%,C:16.3%,A+T:67.5%,DNA碱基序列与BOLD(The Barcode of Life Data system)中收录蛆症异蚤蝇的序列相似度为100%,BOLD库中结果有20个序列推荐支持。综合形态和DNA条形码的鉴定结果,确定拟寄生种类为蛆症异蚤蝇(Megaselia scalaris)。双叉犀金龟拟寄生蚤蝇的鉴定为鞘翅目的生物防治提供了新证据。展开更多
基金supported by the National Natural Science Foundation of China(30270187)
文摘A new Megaselia species： Megaselia angustirostris Fang ＆ Liu, sp. nov., is described and illustrated and two species of the genus, M. nigra （Meigen） and M. albicaudata （Wood）, are reported for the first time from China. The type specimens are deposited in College of Biological and Environmental Engineering, Shenyang University.
基金This project was supported by the National Natural Science Foundation of China (30270187)
文摘Megaselia wuzhiensis, sp. nov., from Mt. Wuzhi, Hainan Province is described. It is similar to M. atricornis Beyer, but differs in Costal Ratios and hypopygium.
基金supported by the National Natural Science Foundation of China (31071965)
文摘Two new species of the genus Megaselia, M. pennisetalis sp. nov. and M. tarsocrassa sp. nov., are described and illustrated. The type specimens are deposited in Liaoning Key Laboratory of Urban Integrated Pest Management and Ecological Security, Shenyang University.
文摘目的:建立应用DNA条形码技术鉴定蛆症异蚤蝇的方法。方法:分别提取在口岸检疫中截获的双翅目幼虫和成虫的基因组DNA,利用节肢动物线粒体细胞色素氧化酶I(COI)的通用引物进行DNA扩增。PCR产物直接测序,测序引物同扩增引物,得到清晰可读的665 bp COI的DNA片段碱基序列。与GenBank中相近的序列进行比较,并用ClustalW2在线建立NJ系统发育树。结果:成虫和幼虫COI的DNA序列100%相同,与GenBank中公布的长度为559 bp的蛆症异蚤蝇的COI相应片段相似性高达98.9%,与同属其他种类的可比片段的DNA序列相似性则低于90%。结论:根据结果可以判断所截获的双翅目昆虫为蛆症异蚤蝇。本文的研究再一次证明了DNA条形码技术可以直接应用于昆虫种类的鉴定。
文摘在双叉犀金龟人工饲养过程中,连续2年出现了的拟寄生现象,对拟寄生的寄主种类进行鉴定。形态鉴定:寄主的幼虫、蛹和成虫均符合蛆症异蚤蝇(Megaselia scalaris)的形态特征;DNA条形码鉴定:扩增的842 bp碱基含量分别为A:29.6%,T:37.9%,G:16.2%,C:16.3%,A+T:67.5%,DNA碱基序列与BOLD(The Barcode of Life Data system)中收录蛆症异蚤蝇的序列相似度为100%,BOLD库中结果有20个序列推荐支持。综合形态和DNA条形码的鉴定结果,确定拟寄生种类为蛆症异蚤蝇(Megaselia scalaris)。双叉犀金龟拟寄生蚤蝇的鉴定为鞘翅目的生物防治提供了新证据。
